Standard name | |
---|---|
Human Ortholog | |
Description | Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; binds to nascent Rpl5p during translation; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 12 | 4 | 7 | 0 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 2 | 4 | 8 | – | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 62 | 25 | 33 | 19 | 22 | 30 | 41 | 37 | 28 | 21 | 22 | 15 | – | 40 | 36 | 17 | 23 | 18 | 34 | 11 | 14 | 8 | 4 | 10 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 5 | 6 | 2 | 0 | 1 | 1 | 0 | 1 | 4 |
Endosome | 2 | 4 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 0 | 3 | – | 2 | 1 | 2 | 0 | 3 | 5 | 5 | 1 | 1 | 3 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 6 | 0 | 1 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 5 | 0 | 0 | 1 | 1 | 15 | 25 | 11 | 44 | 16 | 88 | 26 | – | 0 | 1 | 3 | 2 | 0 | 2 | 5 | 2 | 0 | 0 | 2 |
Nucleus | 152 | 161 | 99 | 191 | 226 | 221 | 308 | 240 | 218 | 229 | 186 | 167 | – | 39 | 68 | 157 | 152 | 135 | 288 | 293 | 130 | 24 | 39 | 61 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 0 | 2 | 3 | 1 | 2 | 0 | 1 | 1 | – | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 2 | 2 | 4 | 6 | 9 | 17 | 5 | 18 | 8 | – | 2 | 1 | 5 | 2 | 7 | 4 | 5 | 0 | 0 | 1 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 1 | 4 | 3 | 5 | – | 0 | 2 | 0 | 0 | 0 | 5 | 5 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 230 | 196 | 113 | 16 | 8 | 13 | 30 | 20 | 11 | 17 | 10 | 26 | – | 30 | 20 | 60 | 46 | 39 | 48 | 44 | 43 | 5 | 11 | 34 |
Unique Cell Count | 387 | 340 | 202 | 221 | 257 | 279 | 391 | 314 | 275 | 278 | 258 | 225 | 103 | 123 | 231 | 216 | 194 | 410 | 386 | 206 | 45 | 74 | 137 | |
Labelled Cell Count | 453 | 390 | 246 | 234 | 259 | 291 | 423 | 326 | 326 | 299 | 332 | 260 | 114 | 131 | 252 | 238 | 204 | 410 | 386 | 206 | 45 | 74 | 137 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 11.9 | 10.0 | 9.9 | 9.7 | 7.4 | 7.3 | 7.4 | 6.3 | 6.7 | 5.9 | 6.2 | – | 9.3 | 9.2 | 16.4 | 18.0 | 16.6 | – | 10.2 | 9.8 |
Std Deviation (1e-4) | 1.9 | 2.3 | 1.5 | 2.1 | 2.2 | 1.7 | 1.9 | 1.6 | 1.5 | 1.5 | 1.6 | 1.3 | – | 2.1 | 2.6 | 4.5 | 4.7 | 4.6 | – | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.44 | -0.45 | -0.42 | -0.65 | -0.58 | -0.77 | -0.68 | – | -0.1 | -0.12 | 0.72 | 0.85 | 0.74 | – | 0.03 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.4 | -2.5 | -1.8 | -2.0 | -1.5 | -2.0 | -3.0 | -2.6 | -3.2 | – | 4.4 | 2.8 | -2.9 | -1.7 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.3 | 3.7 | 0 | 6.0 | 3.5 | 9.2 | 5.0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 8.3 | 9.1 | 6.9 | 7.4 | 6.4 | 6.9 | 7.8 | 5.1 | 5.4 | – | -1.8 | 1.1 | 4.0 | 4.5 | 4.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 3.8 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | -10.9 | -12.8 | -12.6 | -13.0 | -12.6 | -12.8 | -12.1 | -12.5 | -9.8 | – | -4.4 | -7.1 | -6.4 | -7.3 | -7.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6011 | 8.3277 | 7.0024 | 7.5008 | 7.3912 | 7.1576 | 5.5471 | 7.8489 | 7.1574 | 6.6817 | 6.2097 | 6.7907 | 6.1508 | 8.429 | 7.3312 | 6.6019 | 6.2706 | 7.3894 |
Actin | 0.0195 | 0.0008 | 0.0054 | 0.0005 | 0 | 0.0001 | 0.0271 | 0 | 0.006 | 0.0304 | 0.0031 | 0.0172 | 0.014 | 0 | 0.0019 | 0.0165 | 0.0202 | 0.002 |
Bud | 0.0003 | 0.0009 | 0.0042 | 0.0001 | 0 | 0.0003 | 0.0006 | 0 | 0.0009 | 0.0006 | 0.0017 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0015 | 0.0013 | 0.0001 |
Bud Neck | 0.0011 | 0.0004 | 0.0038 | 0.0003 | 0.0012 | 0.0021 | 0.0031 | 0.0001 | 0.0025 | 0.0048 | 0.001 | 0.0026 | 0.0012 | 0.0001 | 0.0002 | 0.0008 | 0.0025 | 0.0012 |
Bud Periphery | 0.0009 | 0.0003 | 0.0044 | 0.0001 | 0.0001 | 0.0013 | 0.001 | 0 | 0.0018 | 0.0006 | 0.0039 | 0.0011 | 0.0002 | 0 | 0.0003 | 0.0011 | 0.003 | 0.0002 |
Bud Site | 0.0009 | 0.002 | 0.0135 | 0.0001 | 0.0001 | 0.0003 | 0.0037 | 0 | 0.0061 | 0.0026 | 0.0019 | 0.0008 | 0.0086 | 0.0001 | 0.0017 | 0.0011 | 0.001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0006 | 0 | 0 | 0.0005 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0005 | 0 |
Cytoplasm | 0.0048 | 0.0031 | 0.0009 | 0.0011 | 0.0006 | 0.0008 | 0.007 | 0.0065 | 0.0013 | 0.0053 | 0.0141 | 0.0012 | 0.0041 | 0.0017 | 0.0024 | 0.0632 | 0.0054 | 0.0006 |
Cytoplasmic Foci | 0.0123 | 0.0002 | 0.0067 | 0.0003 | 0 | 0 | 0.011 | 0 | 0.0019 | 0.0094 | 0.0108 | 0.0058 | 0.0241 | 0 | 0.006 | 0.0073 | 0.0074 | 0 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.004 | 0.0002 | 0.0009 | 0.0006 | 0.0001 | 0.0004 | 0.0036 | 0.0001 | 0.0008 | 0.0036 | 0.0055 | 0.0015 | 0.0009 | 0 | 0.0004 | 0.0008 | 0.0028 | 0.0004 |
Endosome | 0.0126 | 0.0029 | 0.0072 | 0.0004 | 0.0001 | 0.0001 | 0.0171 | 0.0001 | 0.0051 | 0.0168 | 0.0332 | 0.0128 | 0.0094 | 0 | 0.0091 | 0.0179 | 0.0067 | 0.0005 |
Golgi | 0.0014 | 0.0028 | 0.0021 | 0 | 0 | 0 | 0.007 | 0 | 0.0036 | 0.0069 | 0.0098 | 0.0052 | 0.0044 | 0 | 0.0077 | 0.0443 | 0.0067 | 0.0001 |
Lipid Particles | 0.0077 | 0.0003 | 0.004 | 0 | 0 | 0 | 0.022 | 0 | 0.0027 | 0.0042 | 0.0267 | 0.006 | 0.0249 | 0 | 0.0044 | 0.0054 | 0.0081 | 0.0001 |
Mitochondria | 0.0259 | 0.0007 | 0.0077 | 0.0007 | 0.0002 | 0.0005 | 0.0131 | 0.0001 | 0.006 | 0.0023 | 0.0116 | 0.0048 | 0.0019 | 0.0002 | 0.015 | 0.0153 | 0.0101 | 0.0043 |
None | 0.0054 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0035 | 0 | 0.0003 | 0.0009 | 0.0021 | 0.0005 | 0.0014 | 0 | 0.001 | 0.001 | 0.0007 | 0.0001 |
Nuclear Periphery | 0.0201 | 0.0042 | 0.0054 | 0.0267 | 0.0037 | 0.0012 | 0.0243 | 0.0028 | 0.0049 | 0.0054 | 0.0403 | 0.0041 | 0.0115 | 0.0024 | 0.0061 | 0.0046 | 0.021 | 0.0102 |
Nucleolus | 0.0222 | 0.0058 | 0.0087 | 0.0084 | 0.0087 | 0.0236 | 0.0168 | 0.0044 | 0.0069 | 0.0119 | 0.0168 | 0.0193 | 0.0066 | 0.0068 | 0.0168 | 0.0053 | 0.0275 | 0.0287 |
Nucleus | 0.8285 | 0.9707 | 0.9107 | 0.9294 | 0.9701 | 0.9642 | 0.8095 | 0.9845 | 0.942 | 0.8814 | 0.7292 | 0.9014 | 0.8482 | 0.9872 | 0.9085 | 0.7824 | 0.8383 | 0.9476 |
Peroxisomes | 0.0096 | 0.0001 | 0.0069 | 0 | 0 | 0 | 0.0102 | 0 | 0.0014 | 0.0064 | 0.0066 | 0.0047 | 0.0265 | 0 | 0.006 | 0.0013 | 0.0239 | 0 |
Punctate Nuclear | 0.0137 | 0.0034 | 0.0006 | 0.0301 | 0.0141 | 0.0016 | 0.0129 | 0.0012 | 0.0022 | 0.0053 | 0.0109 | 0.0057 | 0.011 | 0.0012 | 0.0052 | 0.0276 | 0.0062 | 0.003 |
Vacuole | 0.0055 | 0.0008 | 0.0039 | 0.0004 | 0.0007 | 0.0024 | 0.0035 | 0.0002 | 0.0023 | 0.0006 | 0.0447 | 0.0024 | 0.0006 | 0.0002 | 0.0048 | 0.0012 | 0.0045 | 0.0004 |
Vacuole Periphery | 0.0029 | 0.0004 | 0.0019 | 0.0005 | 0.0002 | 0.0004 | 0.002 | 0 | 0.0006 | 0.0004 | 0.0259 | 0.0019 | 0.0003 | 0.0002 | 0.002 | 0.0009 | 0.0022 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.7852 | 44.4743 | 37.7164 | 30.9419 | 36.4756 | 27.8214 | 34.7966 | 28.7244 | 17.6343 | 38.51 |
Translational Efficiency | 0.9565 | 1.0852 | 1.0135 | 0.6931 | 0.7867 | 1.0771 | 0.9966 | 0.6538 | 0.8104 | 0.8182 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
147 | 1373 | 661 | 1538 | 1749 | 1266 | 1249 | 881 | 1896 | 2639 | 1910 | 2419 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 718.63 | 983.52 | 945.61 | 1278.86 | 969.19 | 1037.57 | 1161.88 | 1343.06 | 949.76 | 1009.45 | 1087.03 | 1302.24 |
Standard Deviation | 103.03 | 238.34 | 195.94 | 253.21 | 145.25 | 185.45 | 198.47 | 252.74 | 157.40 | 216.30 | 222.78 | 254.92 |
Intensity Change Log 2 | — | 0.452705 | 0.395996 | 0.831537 | — | 0.098357 | 0.261610 | 0.470672 | — | 0.259973 | 0.320365 | 0.635463 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000550 | 0.000542 | 0.000839 | 0.000978 | 0.000380 | 0.000446 | 0.000648 | 0.000624 | 0.000393 | 0.000496 | 0.000714 | 0.000849 |
Bud Neck | 0.024266 | 0.010916 | 0.008734 | 0.029741 | 0.007604 | 0.009687 | 0.015449 | 0.024272 | 0.008895 | 0.010326 | 0.013125 | 0.027749 |
Bud Site | 0.011442 | 0.003318 | 0.002574 | 0.004869 | 0.001478 | 0.002939 | 0.002680 | 0.006825 | 0.002251 | 0.003136 | 0.002643 | 0.005581 |
Cell Periphery | 0.000290 | 0.000548 | 0.000191 | 0.000356 | 0.000290 | 0.000303 | 0.000207 | 0.000144 | 0.000290 | 0.000431 | 0.000201 | 0.000279 |
Cytoplasm | 0.195989* | 0.054145 | 0.043001 | 0.006197 | 0.036388 | 0.017605 | 0.007584 | 0.003101 | 0.048762 | 0.036616 | 0.019841 | 0.005070 |
Cytoplasmic Foci | 0.045741 | 0.000937 | 0.000770 | 0.001598 | 0.000865 | 0.001101 | 0.000045 | 0.001170 | 0.004344 | 0.001016 | 0.000296 | 0.001442 |
Eisosomes | 0.000082 | 0.000019 | 0.000018 | 0.000043 | 0.000018 | 0.000025 | 0.000030 | 0.000032 | 0.000023 | 0.000021 | 0.000026 | 0.000039 |
Endoplasmic Reticulum | 0.004535 | 0.004887 | 0.005389 | 0.002176 | 0.006116 | 0.006481 | 0.003786 | 0.001358 | 0.005993 | 0.005651 | 0.004341 | 0.001878 |
Endosome | 0.011680 | 0.000703 | 0.000474 | 0.002594 | 0.000613 | 0.001081 | 0.000266 | 0.003052 | 0.001471 | 0.000884 | 0.000338 | 0.002761 |
Golgi | 0.000960 | 0.000792 | 0.000231 | 0.003398 | 0.000139 | 0.000388 | 0.000076 | 0.000676 | 0.000203 | 0.000598 | 0.000129 | 0.002407 |
Lipid Particles | 0.005503 | 0.000207 | 0.000588 | 0.000263 | 0.000239 | 0.000219 | 0.000076 | 0.000120 | 0.000647 | 0.000213 | 0.000253 | 0.000211 |
Mitochondria | 0.006889 | 0.001140 | 0.001520 | 0.003850 | 0.001737 | 0.001555 | 0.001543 | 0.003093 | 0.002137 | 0.001339 | 0.001535 | 0.003575 |
Mitotic Spindle | 0.002915 | 0.001219 | 0.000713 | 0.012964 | 0.001707 | 0.004987 | 0.002221 | 0.014477 | 0.001801 | 0.003026 | 0.001699 | 0.013515 |
None | 0.008178 | 0.008314 | 0.004323 | 0.005752 | 0.004076 | 0.003665 | 0.002975 | 0.002516 | 0.004394 | 0.006084 | 0.003442 | 0.004573 |
Nuclear Periphery | 0.002548 | 0.000680 | 0.000711 | 0.002075 | 0.001052 | 0.000850 | 0.000926 | 0.000549 | 0.001168 | 0.000762 | 0.000851 | 0.001519 |
Nuclear Periphery Foci | 0.001101 | 0.000545 | 0.000739 | 0.001513 | 0.000766 | 0.000792 | 0.000149 | 0.000290 | 0.000792 | 0.000663 | 0.000353 | 0.001067 |
Nucleolus | 0.005987 | 0.002426 | 0.001619 | 0.006868 | 0.002243 | 0.004149 | 0.001735 | 0.006860 | 0.002533 | 0.003253 | 0.001695 | 0.006865 |
Nucleus | 0.626541* | 0.903959* | 0.921820* | 0.908759* | 0.930679* | 0.938816* | 0.956479* | 0.925327* | 0.907098* | 0.920680* | 0.944485* | 0.914793* |
Peroxisomes | 0.005359 | 0.000241 | 0.000262 | 0.000411 | 0.000137 | 0.000266 | 0.000087 | 0.000500 | 0.000542 | 0.000253 | 0.000148 | 0.000443 |
Vacuole | 0.037793 | 0.004086 | 0.005182 | 0.003510 | 0.003114 | 0.004031 | 0.002598 | 0.002844 | 0.005802 | 0.004060 | 0.003492 | 0.003267 |
Vacuole Periphery | 0.001651 | 0.000378 | 0.000301 | 0.002086 | 0.000361 | 0.000614 | 0.000440 | 0.002171 | 0.000461 | 0.000491 | 0.000392 | 0.002117 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.10 | -1.35 | -1.23 | -3.35 | 0.12 | -1.40 | -7.88 | -6.41 | -5.89 | -0.42 | -2.13 | -8.58 | -5.77 | -5.00 | -0.68 |
Bud Neck | 1.78 | 2.00 | -1.23 | -8.62 | -8.32 | -2.37 | -7.32 | -9.91 | -8.49 | -4.81 | -2.17 | -4.99 | -12.99 | -11.92 | -8.92 |
Bud Site | 2.29 | 2.37 | 2.09 | -0.84 | -1.01 | -1.84 | -1.58 | -2.70 | -1.52 | -1.83 | -1.74 | -0.71 | -3.29 | -1.75 | -2.28 |
Cell Periphery | -2.23 | 1.45 | 0.38 | 2.29 | -0.69 | -0.17 | 3.17 | 4.31 | 3.17 | 1.55 | -2.26 | 3.39 | 1.40 | 2.85 | -0.39 |
Cytoplasm | 6.90 | 7.65 | 9.40 | 12.33 | 8.10 | 6.17 | 11.34 | 13.65 | 7.66 | 4.19 | 3.43 | 9.47 | 15.07 | 13.99 | 8.24 |
Cytoplasmic Foci | 6.49 | 6.53 | 6.49 | 0.14 | -0.70 | -0.55 | 5.24 | 0.74 | 1.02 | -2.51 | 5.12 | 6.60 | 5.45 | 0.73 | -2.68 |
Eisosomes | 6.36 | 6.13 | 4.38 | -7.76 | -6.35 | -3.08 | -8.85 | -8.66 | -4.36 | -1.22 | 1.32 | -2.85 | -6.63 | -8.38 | -4.86 |
Endoplasmic Reticulum | -0.22 | -1.06 | 2.19 | 11.36 | 10.36 | -0.87 | 6.34 | 12.30 | 11.14 | 8.21 | 1.13 | 4.21 | 14.48 | 15.72 | 13.85 |
Endosome | 3.02 | 3.08 | 2.75 | -2.26 | -2.90 | -2.06 | 4.22 | -1.34 | -0.81 | -1.74 | 1.82 | 3.80 | -0.45 | -1.89 | -3.31 |
Golgi | 0.17 | 1.11 | -1.11 | -1.31 | -2.22 | -1.33 | 0.84 | -2.17 | -0.43 | -2.81 | -1.31 | 0.79 | -2.42 | -1.50 | -2.58 |
Lipid Particles | 3.19 | 2.94 | 3.21 | 0.43 | 1.23 | 0.73 | 3.13 | 2.58 | 1.85 | -0.16 | 3.19 | 2.38 | 3.60 | 1.22 | 0.95 |
Mitochondria | 1.50 | 1.38 | 0.86 | -4.22 | -3.32 | 0.21 | -0.10 | -1.59 | -2.51 | -2.50 | 1.09 | 0.58 | -1.56 | -4.68 | -4.04 |
Mitotic Spindle | 0.91 | 0.84 | -3.32 | -4.75 | -4.61 | -1.89 | -1.02 | -3.68 | -2.38 | -3.27 | -1.20 | -0.59 | -5.53 | -4.78 | -5.33 |
None | 0.03 | 1.94 | 1.68 | 3.04 | -0.46 | 0.62 | 1.76 | 2.47 | 1.81 | 0.96 | -2.28 | 1.78 | 0.85 | 2.93 | -0.64 |
Nuclear Periphery | 1.56 | 1.47 | 0.69 | -2.87 | -2.59 | 1.53 | -0.27 | 2.15 | 0.77 | 1.44 | 3.07 | 0.88 | -0.72 | -2.50 | -1.29 |
Nuclear Periphery Foci | 1.16 | 0.06 | 0.29 | -1.00 | 0.18 | 0.38 | 3.02 | 2.73 | 1.91 | -0.55 | 1.00 | 1.55 | 0.87 | 0.02 | -0.66 |
Nucleolus | 1.66 | 1.92 | -0.75 | -5.03 | -5.16 | -2.01 | 0.62 | -4.84 | -3.45 | -5.15 | -1.06 | 1.29 | -6.29 | -5.62 | -7.52 |
Nucleus | -9.85 | -10.49 | -10.34 | -1.98 | 0.74 | -1.58 | -5.37 | 0.90 | 2.02 | 4.48 | -2.58 | -7.28 | -2.33 | 0.11 | 4.95 |
Peroxisomes | 2.73 | 2.71 | 2.69 | -0.86 | -0.37 | -1.24 | 0.93 | -2.06 | -1.19 | -2.41 | 1.82 | 2.44 | 1.01 | -1.42 | -2.68 |
Vacuole | 4.30 | 4.08 | 4.50 | 1.67 | 3.73 | -0.73 | -0.27 | 0.90 | 1.46 | 1.02 | 1.96 | 1.63 | 3.58 | 2.14 | 2.48 |
Vacuole Periphery | 2.62 | 2.83 | 0.41 | -2.14 | -2.37 | -1.04 | 0.12 | -1.64 | -1.21 | -1.63 | -0.22 | 0.72 | -2.34 | -2.31 | -2.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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