Standard name | |
---|---|
Human Ortholog | |
Description | Sterol transport protein and functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes; yeast NPC2 can complement mutations in human NPC2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 18 | 3 | 35 | 42 | 31 | 65 | 71 | 54 | 69 | 59 | 74 | 53 | 216 | 355 | 52 | 36 | 33 | 1 | 5 | 1 | 2 | 9 | 7 |
Endoplasmic Reticulum | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 21 | 14 | 18 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 9 | 6 | 0 | 3 | 13 | 2 | 10 | 5 | 1 | 4 | 0 | 1 | 37 | 18 | 42 | 20 | 27 | 14 | 8 | 7 | 0 | 12 | 23 | 28 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 |
Mitochondria | 26 | 32 | 3 | 2 | 3 | 42 | 3 | 36 | 17 | 16 | 13 | 14 | 9 | 5 | 2 | 30 | 18 | 9 | 4 | 2 | 3 | 2 | 6 | 3 |
Nucleus | 41 | 44 | 31 | 72 | 80 | 98 | 139 | 219 | 119 | 109 | 73 | 62 | 12 | 20 | 12 | 146 | 133 | 171 | 1 | 6 | 0 | 2 | 2 | 5 |
Nuclear Periphery | 2 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 8 | 5 |
Nucleolus | 4 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 3 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 7 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 104 | 187 | 42 | 129 | 225 | 173 | 308 | 423 | 254 | 291 | 210 | 201 | 151 | 230 | 188 | 174 | 206 | 294 | 151 | 231 | 96 | 273 | 466 | 445 |
Unique Cell Count | 157 | 240 | 67 | 176 | 305 | 285 | 440 | 629 | 365 | 404 | 289 | 284 | 206 | 405 | 518 | 365 | 347 | 446 | 174 | 260 | 104 | 300 | 532 | 513 |
Labelled Cell Count | 195 | 292 | 82 | 245 | 369 | 350 | 528 | 758 | 448 | 497 | 358 | 356 | 270 | 492 | 608 | 444 | 440 | 544 | 174 | 260 | 104 | 300 | 532 | 513 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.7 | 5.1 | 6.4 | 8.8 | 6.6 | 9.1 | 8.3 | 8.5 | 8.6 | 7.8 | 8.1 | 5.5 | 5.2 | 5.1 | 6.6 | 7.4 | 7.6 | 5.2 | 5.2 | 5.4 |
Std Deviation (1e-4) | 1.0 | 1.3 | 0.9 | 1.6 | 2.1 | 2.3 | 2.7 | 2.5 | 2.5 | 2.5 | 2.3 | 2.2 | 1.3 | 1.2 | 1.1 | 1.8 | 2.2 | 2.0 | 1.0 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.34 | 0.79 | 0.39 | 0.84 | 0.71 | 0.74 | 0.77 | 0.63 | 0.68 | 0.11 | 0.03 | 0.02 | 0.38 | 0.56 | 0.59 | 0.03 | 0.04 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.0 | 2.1 | 1.6 | 2.3 | 1.7 | 2.3 | 2.7 | 3.1 | 3.8 | 3.7 | 7.4 | 10.1 | 2.2 | 1.5 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 |
Nucleus | -0.8 | -3.2 | -1.8 | -2.4 | -1.9 | -2.2 | -3.2 | -3.4 | -4.1 | -7.9 | -10.1 | 0 | -1.0 | -1.2 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.6 | 1.8 | -0.3 | 1.2 | 0.8 | 1.1 | 1.6 | 1.6 | 1.3 | 1.7 | -0.9 | -4.2 | -2.3 | -0.5 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0086 | 1.4767 | 0.9308 | 0.5884 | 0.7412 | 1.0855 | 1.3598 | 3.2148 | 2.1593 | 2.0988 | 2.22 | 2.4676 | 2.8041 | 4.1557 | 3.7402 | 3.3466 | 3.434 | 3.7337 |
Actin | 0.0015 | 0 | 0 | 0 | 0 | 0 | 0.0046 | 0 | 0.0004 | 0.002 | 0.0045 | 0.0003 | 0.021 | 0.0001 | 0.0046 | 0.0013 | 0.0247 | 0.0045 |
Bud | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0006 | 0.0003 | 0.0009 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0007 | 0.0004 | 0.0009 | 0.0004 |
Bud Neck | 0.0008 | 0.0017 | 0.001 | 0.001 | 0.0014 | 0.0012 | 0.0017 | 0.0011 | 0.0009 | 0.0012 | 0.0014 | 0.0019 | 0.0015 | 0.0012 | 0.0013 | 0.0074 | 0.0013 | 0.002 |
Bud Periphery | 0.0011 | 0.0012 | 0.0009 | 0.0006 | 0.0009 | 0.0012 | 0.0024 | 0.001 | 0.0009 | 0.0019 | 0.0012 | 0.0012 | 0.002 | 0.0008 | 0.0017 | 0.0014 | 0.0025 | 0.0014 |
Bud Site | 0.0007 | 0.0017 | 0.0013 | 0.0016 | 0.0012 | 0.0005 | 0.0017 | 0.0032 | 0.001 | 0.0012 | 0.0014 | 0.0007 | 0.0013 | 0.0009 | 0.0045 | 0.0013 | 0.0013 | 0.001 |
Cell Periphery | 0.0023 | 0.0027 | 0.0027 | 0.0018 | 0.0023 | 0.0041 | 0.0026 | 0.0026 | 0.0023 | 0.0027 | 0.0018 | 0.0035 | 0.0022 | 0.0042 | 0.0036 | 0.0019 | 0.0015 | 0.0045 |
Cytoplasm | 0.0044 | 0.007 | 0.0044 | 0.0017 | 0.0016 | 0.0027 | 0.0032 | 0.0018 | 0.0024 | 0.001 | 0.0009 | 0.0012 | 0.0066 | 0.0055 | 0.0082 | 0.0009 | 0.0017 | 0.0018 |
Cytoplasmic Foci | 0.0096 | 0.009 | 0.0092 | 0.0108 | 0.004 | 0.0059 | 0.0082 | 0.0035 | 0.0053 | 0.0038 | 0.0059 | 0.0047 | 0.0158 | 0.0035 | 0.0133 | 0.0038 | 0.0035 | 0.0054 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0019 | 0.0022 | 0.0008 | 0.0009 | 0.0022 | 0.0043 | 0.0032 | 0.003 | 0.0006 | 0.001 | 0.0015 | 0.0049 | 0.0055 | 0.0026 | 0.0019 | 0.0023 | 0.0021 |
Endosome | 0.2104 | 0.1173 | 0.1827 | 0.2143 | 0.1488 | 0.1157 | 0.2176 | 0.1254 | 0.1994 | 0.1771 | 0.1635 | 0.1214 | 0.2337 | 0.1139 | 0.2113 | 0.2141 | 0.1771 | 0.1349 |
Golgi | 0.0054 | 0.0024 | 0.0053 | 0.0076 | 0.0044 | 0.0023 | 0.0095 | 0.0027 | 0.0098 | 0.0074 | 0.0068 | 0.0045 | 0.0111 | 0.0032 | 0.0148 | 0.0113 | 0.0076 | 0.0061 |
Lipid Particles | 0.0323 | 0.0068 | 0.0369 | 0.006 | 0.0127 | 0.0155 | 0.0403 | 0.008 | 0.0221 | 0.0055 | 0.0191 | 0.0199 | 0.0326 | 0.0103 | 0.03 | 0.0127 | 0.0055 | 0.0161 |
Mitochondria | 0.0218 | 0.0094 | 0.0287 | 0.0056 | 0.0157 | 0.0123 | 0.0316 | 0.008 | 0.0382 | 0.0548 | 0.0172 | 0.0209 | 0.0139 | 0.0098 | 0.0476 | 0.0444 | 0.0036 | 0.0184 |
None | 0.0027 | 0.0006 | 0.0045 | 0.0001 | 0.0004 | 0.0037 | 0.0037 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0054 | 0.005 | 0.0077 | 0.0002 | 0.0012 | 0.0003 |
Nuclear Periphery | 0.0056 | 0.0033 | 0.0148 | 0.0013 | 0.0016 | 0.0055 | 0.0188 | 0.0085 | 0.0125 | 0.0012 | 0.0022 | 0.0077 | 0.0075 | 0.0079 | 0.004 | 0.0037 | 0.0049 | 0.0056 |
Nucleolus | 0.008 | 0.0069 | 0.0179 | 0.002 | 0.0137 | 0.004 | 0.0079 | 0.006 | 0.0041 | 0.0029 | 0.0038 | 0.0098 | 0.0038 | 0.0038 | 0.0033 | 0.0041 | 0.003 | 0.0103 |
Nucleus | 0.0135 | 0.0047 | 0.0195 | 0.0019 | 0.0062 | 0.006 | 0.0145 | 0.0071 | 0.0037 | 0.0022 | 0.0036 | 0.0076 | 0.0104 | 0.0057 | 0.0027 | 0.0029 | 0.0034 | 0.0128 |
Peroxisomes | 0.0021 | 0.0011 | 0.0022 | 0.0012 | 0.0012 | 0.0008 | 0.0069 | 0.0005 | 0.0016 | 0.0015 | 0.01 | 0.0026 | 0.0078 | 0.0007 | 0.0045 | 0.0015 | 0.0012 | 0.0019 |
Punctate Nuclear | 0.0007 | 0.0001 | 0.0014 | 0.0001 | 0.0009 | 0.0003 | 0.0016 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0015 | 0.0062 | 0.0009 | 0.0004 | 0.0006 | 0.0006 | 0.0014 |
Vacuole | 0.48 | 0.6529 | 0.4947 | 0.5732 | 0.6691 | 0.6317 | 0.4447 | 0.6402 | 0.4919 | 0.6051 | 0.6208 | 0.61 | 0.4447 | 0.6466 | 0.459 | 0.5109 | 0.6352 | 0.6108 |
Vacuole Periphery | 0.1944 | 0.1691 | 0.1693 | 0.1681 | 0.113 | 0.1843 | 0.1733 | 0.1762 | 0.1991 | 0.1267 | 0.1337 | 0.1785 | 0.1668 | 0.1701 | 0.1739 | 0.1733 | 0.1168 | 0.1583 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.1453 | 149.2792 | 103.7607 | 136.7326 | 104.1442 | 118.3074 | 150.4584 | 146.3599 | 160.5382 | 132.2715 |
Translational Efficiency | 1.9224 | 1.2303 | 2.063 | 1.5869 | 1.532 | 2.099 | 1.4556 | 1.7203 | 1.8086 | 1.6372 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
739 | 256 | 444 | 125 | 2083 | 2366 | 148 | 1512 | 2822 | 2622 | 592 | 1637 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 754.72 | 841.04 | 907.06 | 838.14 | 748.45 | 811.28 | 910.86 | 862.17 | 750.09 | 814.19 | 908.01 | 860.34 |
Standard Deviation | 105.56 | 122.35 | 106.22 | 95.37 | 89.80 | 99.42 | 113.41 | 107.80 | 94.22 | 102.27 | 108.07 | 107.10 |
Intensity Change Log 2 | — | 0.156233 | 0.265256 | 0.151250 | — | 0.116294 | 0.283323 | 0.204066 | — | 0.136485 | 0.274281 | 0.177790 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000028 | 0.000031 | 0.000286 | 0.000081 | 0.000025 | 0.000021 | 0.000387 | 0.000232 | 0.000025 | 0.000022 | 0.000311 | 0.000221 |
Bud Neck | 0.014068 | 0.020868 | 0.015645 | 0.022212 | 0.011117 | 0.015251 | 0.006569 | 0.015538 | 0.011889 | 0.015799 | 0.013376 | 0.016048 |
Bud Site | 0.005596 | 0.014927 | 0.014293 | 0.004544 | 0.005149 | 0.005757 | 0.009455 | 0.011416 | 0.005266 | 0.006652 | 0.013083 | 0.010892 |
Cell Periphery | 0.001627 | 0.000370 | 0.000094 | 0.000260 | 0.000352 | 0.000998 | 0.000541 | 0.000072 | 0.000686 | 0.000937 | 0.000206 | 0.000086 |
Cytoplasm | 0.011312 | 0.043984 | 0.233622* | 0.166851* | 0.042558 | 0.012438 | 0.182326* | 0.458542* | 0.034376 | 0.015518 | 0.220798* | 0.436268* |
Cytoplasmic Foci | 0.006719 | 0.021894 | 0.061634 | 0.080812 | 0.012245 | 0.004052 | 0.052059 | 0.062355 | 0.010798 | 0.005794 | 0.059241 | 0.063764 |
Eisosomes | 0.000017 | 0.000017 | 0.000059 | 0.000243 | 0.000019 | 0.000020 | 0.000575 | 0.000034 | 0.000019 | 0.000020 | 0.000188 | 0.000050 |
Endoplasmic Reticulum | 0.002723 | 0.001250 | 0.003836 | 0.001024 | 0.003070 | 0.005552 | 0.005727 | 0.001523 | 0.002979 | 0.005132 | 0.004308 | 0.001485 |
Endosome | 0.050422 | 0.070474 | 0.082856 | 0.028533 | 0.033031 | 0.034930 | 0.046464 | 0.056151 | 0.037585 | 0.038401 | 0.073758 | 0.054042 |
Golgi | 0.000645 | 0.000570 | 0.000547 | 0.000316 | 0.000517 | 0.000883 | 0.000683 | 0.001731 | 0.000550 | 0.000853 | 0.000581 | 0.001623 |
Lipid Particles | 0.001591 | 0.000510 | 0.005030 | 0.005265 | 0.001109 | 0.001241 | 0.006303 | 0.002258 | 0.001235 | 0.001169 | 0.005349 | 0.002488 |
Mitochondria | 0.001216 | 0.000518 | 0.000629 | 0.000450 | 0.001330 | 0.002489 | 0.000682 | 0.000778 | 0.001300 | 0.002297 | 0.000643 | 0.000753 |
Mitotic Spindle | 0.000132 | 0.000111 | 0.018412 | 0.002037 | 0.001058 | 0.000589 | 0.002034 | 0.008534 | 0.000815 | 0.000542 | 0.014317 | 0.008038 |
None | 0.000475 | 0.000071 | 0.002028 | 0.003216 | 0.000292 | 0.000280 | 0.004199 | 0.001824 | 0.000340 | 0.000260 | 0.002571 | 0.001930 |
Nuclear Periphery | 0.000141 | 0.000111 | 0.003283 | 0.000872 | 0.000271 | 0.000619 | 0.004920 | 0.001538 | 0.000237 | 0.000570 | 0.003693 | 0.001487 |
Nuclear Periphery Foci | 0.000211 | 0.000180 | 0.007919 | 0.000978 | 0.000080 | 0.000076 | 0.007007 | 0.001937 | 0.000114 | 0.000087 | 0.007691 | 0.001864 |
Nucleolus | 0.000445 | 0.000941 | 0.007079 | 0.011262 | 0.000505 | 0.001113 | 0.002059 | 0.001986 | 0.000490 | 0.001096 | 0.005824 | 0.002694 |
Nucleus | 0.005146 | 0.006697 | 0.158225* | 0.445559* | 0.015637 | 0.021755 | 0.413658* | 0.116647 | 0.012889 | 0.020285 | 0.222083* | 0.141762* |
Peroxisomes | 0.001104 | 0.001081 | 0.007435 | 0.004726 | 0.000492 | 0.000531 | 0.003684 | 0.002806 | 0.000652 | 0.000585 | 0.006497 | 0.002953 |
Vacuole | 0.879343* | 0.791931* | 0.373228* | 0.219802* | 0.862683* | 0.870494* | 0.247392* | 0.252413* | 0.867045* | 0.862824* | 0.341769* | 0.249923* |
Vacuole Periphery | 0.017040 | 0.023462 | 0.003861 | 0.000960 | 0.008460 | 0.020908 | 0.003275 | 0.001685 | 0.010707 | 0.021158 | 0.003714 | 0.001630 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.40 | -6.38 | -1.96 | -1.89 | 3.20 | 0.23 | -6.58 | -2.08 | -2.13 | 3.04 | 0.26 | -8.83 | -2.16 | -2.21 | 1.96 |
Bud Neck | -1.44 | -0.99 | -0.79 | 0.18 | -0.39 | -2.88 | 3.25 | -2.85 | -0.35 | -5.13 | -2.80 | -1.38 | -2.61 | -0.21 | -1.00 |
Bud Site | -3.85 | -3.31 | 0.17 | 3.04 | 2.60 | -0.59 | -2.47 | -3.92 | -3.58 | 0.03 | -1.54 | -4.08 | -3.95 | -2.67 | 1.48 |
Cell Periphery | 6.72 | 8.76 | 7.81 | 1.62 | -5.19 | -3.95 | -0.39 | 11.70 | 5.76 | 3.74 | -1.63 | 9.40 | 12.04 | 5.85 | 3.83 |
Cytoplasm | -4.31 | -19.14 | -9.15 | -6.50 | 3.34 | 10.04 | -8.82 | -48.41 | -54.49* | -13.57 | 7.36 | -19.34 | -49.52 | -53.02 | -15.62 |
Cytoplasmic Foci | -3.75 | -12.11 | -6.29 | -4.52 | -1.18 | 7.94 | -5.79 | -18.39 | -22.68 | -2.78 | 5.30 | -12.84 | -20.07 | -22.57 | -1.41 |
Eisosomes | -0.19 | -10.39 | -8.70 | -8.65 | -6.75 | -0.35 | -2.49 | -7.64 | -7.85 | 2.38 | -0.63 | -3.34 | -10.83 | -10.50 | 2.51 |
Endoplasmic Reticulum | 4.07 | -3.36 | 3.51 | -0.48 | 7.19 | -5.97 | -3.53 | 6.30 | 10.98 | 4.87 | -5.87 | -4.70 | 7.16 | 10.84 | 8.83 |
Endosome | -2.14 | -2.78 | 3.03 | 4.22 | 5.11 | -0.84 | -0.47 | -4.45 | -3.81 | -1.40 | -0.32 | -4.89 | -2.75 | -2.49 | 2.74 |
Golgi | 0.26 | 0.50 | 1.05 | 1.60 | 1.48 | -1.46 | -0.17 | -1.19 | -0.63 | -1.08 | -1.31 | 0.25 | -1.11 | -0.61 | -1.19 |
Lipid Particles | 1.30 | -3.45 | -2.59 | -4.86 | 0.41 | -0.44 | -6.50 | -7.45 | -3.68 | 4.68 | 0.26 | -7.64 | -4.61 | -4.87 | 5.68 |
Mitochondria | 1.14 | 0.94 | 1.31 | 0.60 | 1.69 | -1.84 | 1.50 | 1.22 | 4.22 | -0.76 | -1.85 | 1.72 | 1.50 | 4.21 | -0.61 |
Mitotic Spindle | 0.27 | -4.49 | -1.41 | -1.41 | 2.37 | 0.85 | -1.66 | -4.84 | -5.17 | -3.54 | 0.62 | -4.45 | -5.19 | -5.37 | 2.22 |
None | 0.98 | -2.00 | -2.28 | -2.91 | -0.80 | 0.08 | -2.34 | -4.67 | -5.08 | 1.02 | 0.51 | -3.35 | -4.86 | -5.69 | 0.59 |
Nuclear Periphery | 0.65 | -9.20 | -5.65 | -5.84 | 6.50 | -1.84 | -1.79 | -13.49 | -6.00 | 0.96 | -1.95 | -6.57 | -14.40 | -6.54 | 3.80 |
Nuclear Periphery Foci | -0.03 | -7.18 | -3.61 | -3.65 | 5.66 | 0.26 | -5.61 | -9.61 | -9.62 | 3.96 | 0.86 | -8.70 | -9.90 | -10.05 | 6.52 |
Nucleolus | -1.54 | -4.58 | -2.41 | -2.28 | -0.39 | -4.85 | -4.36 | -8.26 | -5.41 | -2.24 | -5.15 | -4.80 | -6.99 | -5.22 | 2.60 |
Nucleus | -0.92 | -15.85 | -14.34 | -14.27 | -8.12 | -2.57 | -12.17 | -24.22 | -21.82 | 8.00 | -3.59 | -18.61 | -26.77 | -24.27 | 5.53 |
Peroxisomes | 0.19 | -3.41 | -1.53 | -1.59 | 0.92 | -0.29 | -3.77 | -5.78 | -5.72 | 0.87 | 0.59 | -4.42 | -5.53 | -5.88 | 2.31 |
Vacuole | 5.21 | 34.40* | 22.30 | 17.06 | 3.30 | -1.30 | 21.08 | 74.34* | 78.39* | 2.59 | 0.69 | 42.21* | 80.27* | 79.27* | 6.52 |
Vacuole Periphery | -2.31 | 9.97 | 11.31 | 9.48 | 3.33 | -10.87 | 5.77 | 14.59 | 17.99 | 0.62 | -9.53 | 12.76 | 17.84 | 19.65 | 3.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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