Standard name
Human Ortholog
Description Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 1.0 1.0 0.96 0.9 0.93 – 0.76 0.78 0.74 0.78 0.99 1.0 1.0 0.99 0.98 0.99 0.96 0.92 0.92 0.93 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.07 – 0.37 0.3 0.34 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 2 – 0 0 0 1 0 0 0 0 0 0 0 2 3 2 3 7
Bud 1 0 0 1 0 2 3 – 6 10 9 12 0 0 1 0 0 0 1 4 1 6 17 18
Bud Neck 0 2 2 2 3 2 3 – 4 1 1 5 0 0 0 1 1 0 2 0 1 0 1 4
Bud Site 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 4 3 4 2 4 – 2 3 3 9 2 0 2 3 5 2 0 1 1 0 2 5
Cytoplasm 456 419 374 303 238 252 377 – 281 313 271 524 712 864 917 577 339 358 438 387 342 481 562 593
Endoplasmic Reticulum 1 4 0 0 3 0 2 – 0 1 2 2 1 0 0 32 11 14 1 1 1 1 2 3
Endosome 1 1 1 0 0 1 0 – 0 4 1 2 0 1 0 1 0 1 1 3 1 1 3 3
Golgi 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0
Mitochondria 4 7 0 2 8 31 27 – 138 118 125 193 0 0 0 16 4 1 4 4 5 1 2 6
Nucleus 0 0 0 0 2 2 6 – 4 1 3 12 0 0 0 1 0 2 0 0 0 1 0 2
Nuclear Periphery 0 1 0 0 0 0 0 – 2 1 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 1 0 0 – 0 3 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 1 0 0 0 1 1 – 1 1 1 3 1 0 0 0 0 0 0 2 2 5 7 13
Vac/Vac Membrane 3 1 0 0 2 9 9 – 4 17 13 18 1 0 2 4 9 2 1 4 0 3 3 3
Unique Cell Count 463 431 375 304 249 280 406 370 399 367 671 716 865 919 585 345 361 459 422 370 518 619 683
Labelled Cell Count 469 438 381 312 261 303 434 443 473 429 783 718 865 922 635 369 380 459 422 370 518 619 683


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.2 5.1 4.7 4.7 4.7 4.9 – 3.7 4.4 4.2 4.3 5.3 5.4 4.9 5.1 6.4 6.2 5.7 5.9 5.8
Std Deviation (1e-4) 0.5 0.8 0.9 1.1 1.2 1.3 1.3 – 0.9 1.2 1.0 1.0 1.1 1.1 1.1 0.9 1.2 1.0 1.7 1.6 1.5
Intensity Change (Log2) – – – -0.11 -0.13 -0.12 -0.07 – -0.44 -0.2 -0.3 -0.24 0.06 0.07 -0.06 0.0 0.32 0.28 0.17 0.2 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9471 4.0881 4.155 4.2246 3.887 4.5267 4.9312 5.4578 4.8171 4.4153 5.7923 5.1126 2.5967 2.2032 2.5505 2.682 2.4371 2.2951
Actin 0.0194 0.0008 0.0163 0.0333 0.0044 0.0047 0.0145 0.0003 0.0017 0.0014 0.0004 0.0017 0.0362 0.0014 0.0086 0.0569 0.0533 0.008
Bud 0.0006 0.001 0.0027 0.0013 0.003 0.0007 0.0008 0.0009 0.0027 0.0001 0.0001 0.0009 0.0033 0.0033 0.0036 0.0002 0.0078 0.002
Bud Neck 0.003 0.0004 0.0041 0.0008 0.0004 0.0018 0.0004 0.0001 0.0002 0.0001 0 0.0003 0.0034 0.0004 0.001 0.0051 0.0014 0.0005
Bud Periphery 0.0014 0.0034 0.0048 0.0012 0.0037 0.0013 0.0012 0.0014 0.0032 0.0001 0.0001 0.0009 0.0131 0.0035 0.0014 0.0006 0.0146 0.0019
Bud Site 0.0051 0.0119 0.0044 0.0008 0.0092 0.0008 0.0027 0.0044 0.0015 0.0002 0.0001 0.0001 0.0028 0.0078 0.0075 0.0006 0.0014 0.0002
Cell Periphery 0.0004 0.0006 0.0005 0.0001 0.0006 0.0001 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0002 0.0002 0.0003 0.0001
Cytoplasm 0.1196 0.1913 0.1562 0.1615 0.1366 0.2411 0.1787 0.2319 0.2237 0.2349 0.1422 0.304 0.0737 0.2056 0.0756 0.1403 0.1513 0.1621
Cytoplasmic Foci 0.0227 0.0038 0.0117 0.0054 0.0155 0.0088 0.0079 0.0024 0.0057 0.0064 0.0021 0.0037 0.0374 0.0036 0.0133 0.0029 0.0091 0.0102
Eisosomes 0.0003 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0005 0.0004 0.0001
Endoplasmic Reticulum 0.0016 0.0043 0.0026 0.0015 0.0017 0.0016 0.0085 0.0011 0.0039 0.0039 0.0006 0.0019 0.0023 0.0013 0.0022 0.0006 0.0047 0.0009
Endosome 0.0139 0.0012 0.0037 0.0015 0.0256 0.0019 0.013 0.0002 0.0017 0.0033 0.0003 0.0017 0.0066 0.0005 0.0033 0.0005 0.0133 0.0071
Golgi 0.0048 0.0002 0.0029 0.0015 0.0053 0.0004 0.0024 0.0001 0.0002 0.0002 0 0.0001 0.0033 0.0001 0.0008 0.0005 0.0049 0.0013
Lipid Particles 0.0037 0.0002 0.0037 0.0018 0.0076 0.0022 0.0011 0.0001 0.0004 0.0006 0.0001 0.0001 0.0137 0.0001 0.0019 0.0002 0.005 0.0014
Mitochondria 0.0013 0.001 0.0029 0.0007 0.0201 0.0007 0.002 0.0002 0.0007 0.0003 0.0001 0.0002 0.0026 0.0003 0.0004 0.0002 0.0046 0.0005
None 0.773 0.7738 0.7602 0.7809 0.7519 0.7182 0.7423 0.7527 0.7381 0.743 0.8518 0.6761 0.7379 0.7671 0.8513 0.789 0.657 0.794
Nuclear Periphery 0.0022 0.0013 0.0013 0.0009 0.0014 0.0021 0.0119 0.0004 0.0057 0.0013 0.0004 0.0029 0.009 0.0005 0.0031 0.0002 0.0248 0.0015
Nucleolus 0.0007 0.0002 0.0007 0.0002 0.0002 0.0015 0.0005 0.0002 0.0003 0.0001 0.0001 0.0002 0.0014 0.0002 0.0005 0.0001 0.001 0.0002
Nucleus 0.0014 0.002 0.0018 0.0014 0.0016 0.0043 0.0041 0.0013 0.0062 0.0012 0.0004 0.0018 0.0101 0.0017 0.0023 0.0005 0.0046 0.0009
Peroxisomes 0.0156 0.0001 0.0125 0.0021 0.0056 0.0043 0.0007 0.0001 0.0003 0.0003 0.0001 0.0001 0.0235 0.0002 0.0061 0.0003 0.0048 0.0011
Punctate Nuclear 0.0076 0.0011 0.0057 0.0019 0.0035 0.0029 0.0034 0.0007 0.0018 0.0011 0.0005 0.0028 0.0167 0.0012 0.0161 0.0003 0.0336 0.0056
Vacuole 0.0015 0.001 0.0006 0.0005 0.001 0.0004 0.002 0.0007 0.0011 0.001 0.0002 0.0005 0.0015 0.0006 0.0005 0.0002 0.001 0.0003
Vacuole Periphery 0.0004 0.0002 0.0002 0.0001 0.0008 0.0001 0.0011 0.0001 0.0004 0.0001 0 0.0001 0.0007 0.0001 0.0002 0 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7825 4.2839 11.126 15.7146 8.5433 14.3686 13.2652 13.8864 13.9249 18.8535
Translational Efficiency 1.298 1.7151 0.8817 0.5949 1.4479 0.9991 0.89 0.7842 0.9323 0.6575

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1457 1127 228 172 2090 1245 3078 1209 3547 2372 3306 1381

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.70 769.27 844.46 974.19 657.88 799.43 814.72 879.73 645.07 785.10 816.77 891.49
Standard Deviation 132.96 90.84 80.98 127.56 71.95 83.11 97.04 129.34 102.70 88.17 96.31 132.84
Intensity Change Log 2 — 0.295715 0.430254 0.636428 — 0.281147 0.308480 0.419236 — 0.288273 0.369174 0.529282

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000134 0.000215 0.004267 0.000071 0.000104 0.000080 0.000776 0.000065 0.000118 0.000089 0.001210
Bud Neck 0.008200 0.013398 0.001863 0.003547 0.008022 0.015610 0.006348 0.005290 0.008095 0.014559 0.006039 0.005073
Bud Site 0.001618 0.004193 0.005626 0.011041 0.001597 0.008942 0.008977 0.017587 0.001606 0.006685 0.008746 0.016772
Cell Periphery 0.000331 0.000141 0.000187 0.000261 0.000103 0.000175 0.000085 0.000217 0.000197 0.000159 0.000093 0.000223
Cytoplasm 0.487241* 0.515389* 0.292351* 0.400780* 0.560011* 0.545223* 0.602272* 0.559435* 0.530120* 0.531048* 0.580898* 0.539675*
Cytoplasmic Foci 0.178125 0.178347 0.031349 0.017416 0.142803 0.161655 0.007115 0.028666 0.157312 0.169586 0.008786 0.027265
Eisosomes 0.000610 0.000108 0.000094 0.000093 0.000128 0.000152 0.000017 0.000057 0.000326 0.000131 0.000022 0.000062
Endoplasmic Reticulum 0.001277 0.000822 0.002850 0.006881 0.000602 0.000348 0.000875 0.002420 0.000879 0.000573 0.001012 0.002976
Endosome 0.006805 0.013239 0.004043 0.005156 0.003460 0.008882 0.000907 0.005660 0.004834 0.010952 0.001124 0.005597
Golgi 0.001695 0.003332 0.000075 0.000446 0.000856 0.001474 0.000047 0.001852 0.001200 0.002357 0.000049 0.001677
Lipid Particles 0.012674 0.006168 0.003357 0.003712 0.004805 0.005220 0.000409 0.002328 0.008037 0.005670 0.000612 0.002500
Mitochondria 0.002697 0.004114 0.001895 0.000436 0.002170 0.002866 0.000767 0.001671 0.002387 0.003459 0.000845 0.001517
Mitotic Spindle 0.000191 0.001769 0.000757 0.089294 0.001695 0.002416 0.009583 0.032508 0.001077 0.002109 0.008974 0.039580
None 0.023961 0.005945 0.003422 0.009972 0.004056 0.002459 0.004478 0.006331 0.012232 0.004115 0.004405 0.006784
Nuclear Periphery 0.000636 0.000614 0.001752 0.001760 0.000329 0.000325 0.001243 0.001405 0.000455 0.000463 0.001278 0.001450
Nuclear Periphery Foci 0.000885 0.000436 0.001522 0.008794 0.000275 0.000211 0.000308 0.002227 0.000526 0.000318 0.000392 0.003045
Nucleolus 0.002412 0.002084 0.000962 0.000157 0.000698 0.000705 0.000268 0.000602 0.001402 0.001360 0.000316 0.000546
Nucleus 0.231803* 0.183183* 0.558491* 0.139591* 0.240443* 0.190178* 0.313766* 0.189036* 0.236894* 0.186855* 0.330643* 0.182877*
Peroxisomes 0.003038 0.004005 0.000440 0.005433 0.001931 0.004955 0.000085 0.001025 0.002386 0.004504 0.000110 0.001574
Vacuole 0.035317 0.060961 0.088473 0.290850* 0.025582 0.047286 0.042170 0.139435 0.029581 0.053783 0.045363 0.158293
Vacuole Periphery 0.000429 0.001618 0.000277 0.000114 0.000361 0.000815 0.000199 0.001472 0.000389 0.001196 0.000204 0.001303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.44 -4.23 -1.83 -1.71 -1.46 -1.65 -1.71 -3.70 -3.47 -3.50 -2.85 -3.77 -4.12 -3.73 -3.78
Bud Neck -3.51 9.06 5.13 6.18 -3.18 -5.00 1.84 3.26 6.79 1.11 -6.07 2.78 4.75 8.64 1.03
Bud Site -5.31 -1.27 -2.86 -2.01 -1.25 -4.48 -5.78 -7.55 -1.76 -3.53 -5.69 -5.93 -8.05 -3.62 -3.70
Cell Periphery 5.70 3.72 3.45 -2.13 0.17 -4.85 4.87 -7.84 -0.37 -10.05 2.34 7.82 0.81 -1.98 -10.05
Cytoplasm -2.57 13.30 8.75 9.71 -1.37 1.74 4.99 12.39 9.60 8.90 0.04 3.73 12.33 11.42 9.28
Cytoplasmic Foci 0.10 26.12 33.05 28.41 3.50 -3.17 38.30 32.61 28.42 -10.42 -2.59 52.15 45.21 39.49 -9.54
Eisosomes 5.12 5.06 5.24 1.23 1.50 -1.27 6.06 3.42 11.02 -17.18 4.65 7.13 6.10 11.23 -16.31
Endoplasmic Reticulum 1.99 -6.51 -6.62 -7.51 -2.22 2.26 -6.59 -14.58 -17.79 -11.84 2.63 -5.74 -14.52 -17.86 -12.20
Endosome -5.25 2.81 5.14 8.18 1.71 -4.89 8.15 -0.17 4.64 -6.10 -7.46 13.05 2.82 8.63 -6.20
Golgi -1.75 5.78 5.34 3.66 -2.16 -1.85 6.19 0.34 1.69 -2.30 -2.47 8.33 1.77 3.26 -2.35
Lipid Particles 5.91 8.10 9.66 4.67 1.76 -0.51 10.26 5.48 4.31 -5.94 3.76 15.48 11.17 6.74 -6.04
Mitochondria -1.35 1.13 3.30 5.18 1.04 -0.81 2.53 1.94 2.66 -1.04 -1.61 3.51 3.04 4.78 -0.57
Mitotic Spindle -1.98 -1.04 -2.99 -2.84 -2.94 -0.57 -5.37 -5.54 -5.21 -2.74 -1.32 -6.32 -6.54 -6.11 -3.58
None 6.25 7.41 5.62 -0.33 -2.10 2.42 -0.98 -1.38 -3.85 -0.84 6.38 6.58 5.65 -1.57 -1.47
Nuclear Periphery 0.18 -10.18 -8.46 -8.31 -0.31 0.08 -26.45 -16.88 -16.43 -2.73 -0.21 -25.21 -17.36 -16.66 -2.82
Nuclear Periphery Foci 2.93 -3.02 -6.87 -7.50 -5.26 0.98 -2.91 -12.00 -12.57 -11.39 2.83 -0.25 -12.29 -13.99 -12.72
Nucleolus 0.57 5.65 8.17 4.57 3.49 -0.11 3.66 1.41 1.66 -3.14 0.03 8.70 6.92 4.44 -2.46
Nucleus 6.02 -15.64 6.73 3.15 17.69 7.59 -19.38 1.78 -4.84 18.11 9.76 -24.59 3.11 -4.49 22.49
Peroxisomes -1.54 6.23 1.82 2.69 -0.75 -3.81 8.27 5.84 5.82 -4.48 -4.22 10.69 6.99 8.34 -3.35
Vacuole -8.46 -8.11 -16.95 -15.82 -12.04 -8.81 -18.93 -28.64 -25.00 -21.73 -12.52 -19.31 -31.73 -27.94 -24.65
Vacuole Periphery -4.14 2.19 6.64 5.43 2.44 -2.02 2.88 -1.38 0.58 -2.50 -4.45 4.42 -1.06 2.48 -2.42
Standard name
Human Ortholog
Description Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1
Localization
Cell Percentages cytoplasm (48%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fad1

Fad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available