Standard name
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0.07 0 0 0.05 0 0 0 0 0 0 0.06 0 0.05 0 0 0.05 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0.2 0.12 0.05 0.05 0 0 0 0 0 0 0 0.24 0.3 0.37 0.1 0.19 0.13 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0.09 0.08 0.06 0.11 0 0 0 0 0 0 0 0.17 0.25 0.23 0.26 0.13 0.29 0.14 0.12 0.11 0.1 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0.86 0.62 0.65 0.89 0.8 0.87 0.9 0.88 0.93 0.95 0.96 0.96 0.32 0.08 0.06 0.14 0.16 0.12 0.65 0.59 0.68 0.57 0.58 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.23 0.14 0.15 0.09 0 0 0 0 0 0 0.09 0.07 0.1 0.23 0.22 0.2 0.06 0 0.09 0 0 0
SpindlePole 0.11 0.13 0.14 0.1 0.05 0.08 0.08 0.08 0 0.05 0 0 0.18 0.28 0.27 0.31 0.3 0.27 0.05 0.09 0 0.07 0.09 0.07
Vac/Vac Membrane 0.06 0.11 0.06 0 0.08 0.08 0 0 0 0 0 0 0.1 0.16 0.09 0.15 0.15 0.18 0 0.07 0 0.06 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 0 0 0 0 0 1 22 4 5 1 0 1 3 0 2 3 10 14
Bud 14 4 9 11 12 13 29 24 21 27 29 17 22 12 8 8 4 14 0 2 0 2 7 10
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 6 6 6 0 1 0 1 2 1 4 10 17
Bud Site 1 1 2 3 0 6 23 17 6 13 10 10 1 0 2 2 3 3 – – – – – –
Cell Periphery 0 0 6 3 3 4 3 3 6 2 1 6 16 5 3 8 9 10 0 0 0 0 1 1
Cytoplasm 7 26 28 10 23 15 19 13 7 12 11 6 112 138 196 16 27 19 1 5 1 12 21 18
Endoplasmic Reticulum 6 1 2 1 2 1 2 1 3 3 1 7 1 3 10 3 9 4 1 0 0 0 3 4
Endosome 5 12 18 12 46 11 5 29 5 0 0 2 81 116 121 40 19 42 32 18 26 21 48 59
Golgi 0 0 1 2 0 0 0 0 0 0 0 0 3 3 6 7 7 9 2 0 6 2 5 4
Mitochondria 178 81 148 194 340 350 653 711 496 715 454 608 151 35 34 22 23 18 149 91 167 123 343 369
Nucleus 1 0 1 1 3 11 28 27 10 13 13 4 1 1 1 2 4 3 0 1 0 2 4 8
Nuclear Periphery 2 0 0 0 0 8 17 21 12 19 8 23 0 0 0 1 2 1 1 0 1 0 1 4
Nucleolus 1 1 2 0 4 7 26 15 14 23 8 11 0 1 1 3 2 3 1 1 0 0 3 5
Peroxisomes 7 9 52 30 64 38 23 34 6 19 2 5 41 33 50 36 32 30 13 2 22 3 15 29
SpindlePole 23 17 32 22 23 33 58 63 21 40 12 21 86 129 144 48 43 39 10 13 8 15 50 44
Vac/Vac Membrane 12 14 14 6 34 32 25 36 23 18 18 23 46 72 47 24 21 26 4 10 2 13 52 63
Unique Cell Count 206 130 226 218 424 404 726 808 531 749 473 631 472 464 526 156 144 147 229 155 246 215 590 673
Labelled Cell Count 259 168 315 295 554 529 911 995 631 904 567 744 589 558 634 221 206 222 229 155 246 215 590 673


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.7 4.4 4.6 4.1 4.0 4.0 3.9 3.8 3.5 3.7 5.7 5.6 5.5 6.1 6.7 6.4 5.5 5.6 6.1
Std Deviation (1e-4) 0.6 0.8 1.7 1.8 1.6 1.5 1.3 0.9 0.9 1.0 0.8 0.9 0.8 0.7 1.0 1.4 1.7 1.6 1.0 1.1 1.4
Intensity Change (Log2) – – – -0.09 -0.04 -0.19 -0.24 -0.23 -0.26 -0.3 -0.42 -0.34 0.27 0.25 0.23 0.38 0.5 0.44 0.22 0.25 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 2.7 3.5