Standard name
Human Ortholog
Description Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.05 0 0.05 0.07 0.06 0.05 0.05 0.09 0 0 0.05 0.08 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.94 0.92 0.9 0.8 0.73 0.74 0.75 0.71 0.74 0.98 0.96 0.96 0.98 0.98 0.97 0.94 0.97 0.9 0.9 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.12 0.09 0.07 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.13 0.09 0.18 0.15 0.25 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 2 8 22 13 7 7 14 0 0 0 0 0 1 1 0 0 0 0 0
Bud 0 0 1 0 0 1 1 1 5 8 6 11 0 0 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 1 6 15 19 11 18 36 21 19 15 29 9 6 16 33 8 12 0 0 0 0 0 1
Cytoplasm 259 239 325 266 351 265 304 359 255 276 205 248 244 274 292 405 184 222 244 238 322 134 258 210
Endoplasmic Reticulum 3 7 11 9 14 13 23 58 32 25 25 20 3 5 4 11 5 9 5 3 8 5 7 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 1 1 1 1 0 3 0 0 0 1 0 1 0 1 0 0 0 0 0 1
Mitochondria 4 0 0 1 0 9 48 44 64 54 72 45 0 1 0 1 1 1 2 0 2 2 2 0
Nucleus 0 0 5 2 4 1 8 4 3 2 3 2 0 1 1 0 1 0 0 0 3 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 3 6 11 3 15 26 6 23 5 24 1 1 1 6 0 1 1 1 13 4 4 1
Unique Cell Count 266 245 337 282 382 294 382 492 346 369 288 337 248 286 303 414 187 228 259 247 357 150 279 223
Labelled Cell Count 273 248 351 300 401 308 426 552 400 417 339 393 257 289 315 457 199 247 259 247 357 150 279 223


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.6 28.5 19.8 20.2 16.2 13.2 11.5 11.6 10.2 9.9 9.1 9.8 22.7 22.6 21.4 36.1 36.7 36.0 23.5 23.6 25.1
Std Deviation (1e-4) 3.9 4.8 3.7 3.2 2.6 2.3 2.2 2.1 2.0 1.9 2.0 1.9 4.9 4.1 4.0 8.7 9.9 9.3 5.9 5.0 4.7
Intensity Change (Log2) – – – 0.03 -0.29 -0.58 -0.79 -0.77 -0.96 -1.0 -1.12 -1.02 0.2 0.19 0.11 0.87 0.89 0.86 0.25 0.26 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.9 3.6 0 0 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 2.3 1.5 2.2 3.6 2.9 2.4 2.4 4.0 1.4 0.3 2.4 3.8 0 2.3
Cytoplasm -1.3 -2.6 -3.2 -6.8 -8.7 -8.3 -8.1 -8.8 -8.3 1.4 -0.4 0 1.1 1.3 0.6
Endoplasmic Reticulum -0.1 0.3 0.8 1.7 4.4 3.2 2.1 2.9 1.7 -1.6 -1.2 -1.6 -0.5 -0.4 0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 6.7 5.6 8.3 7.3 9.8 6.9 0 0 0 0 0 0
Nucleus 0 0 0 0.6 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 0 2.6 3.4 0 3.8 0 4.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0131 0.0002 0.0014 0.0023 0.0057 0.0007 0.0095 0.0003 0.0029 0.015 0.0002 0.0011 0.0095 0.0011 0.0152 0.0001 0 0.0002
Bud 0.0005 0.0001 0.0004 0.0026 0.0018 0.0003 0.0005 0.0005 0.0016 0.0011 0.0002 0.0005 0.0034 0.001 0.0004 0.0003 0 0.0003
Bud Neck 0.001 0.0001 0.0004 0.0005 0.0006 0.001 0.0004 0.0002 0.0004 0.0045 0.0004 0.0008 0.0005 0.0002 0.0005 0.0003 0.0002 0.0009
Bud Periphery 0.0004 0 0.0002 0.0048 0.0043 0.0002 0.0005 0.0001 0.0005 0.0004 0.0001 0.0003 0.0055 0.0001 0.0003 0.0011 0 0
Bud Site 0.0015 0.0002 0.0021 0.0006 0.0017 0.0001 0.0015 0.0007 0.0057 0.0064 0.0001 0.0001 0.0024 0.001 0.0005 0.0001 0 0
Cell Periphery 0.0004 0 0.0002 0.0003 0.0004 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0001 0.0006 0.0001 0.0002 0.0007 0 0
Cytoplasm 0.8185 0.9875 0.9228 0.8199 0.8185 0.8885 0.8021 0.9766 0.9027 0.8143 0.8475 0.9185 0.5402 0.9887 0.9437 0.7267 0.9943 0.9752
Cytoplasmic Foci 0.019 0.0021 0.0085 0.0101 0.0235 0.0103 0.0225 0.0042 0.0115 0.0264 0.0154 0.0068 0.0033 0.0026 0.0026 0.0036 0.0026 0.0036
Eisosomes 0.0003 0 0 0 0.0001 0 0.0001 0 0 0.0001 0 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0474 0.004 0.0194 0.0112 0.0114 0.0469 0.0465 0.0057 0.021 0.0045 0.002 0.0364 0.1198 0.0011 0.004 0.0011 0.0002 0.0029
Endosome 0.0396 0.0023 0.0283 0.0598 0.0585 0.0334 0.058 0.0071 0.0148 0.0417 0.108 0.0169 0.0529 0.0017 0.0051 0.0198 0.0015 0.0093
Golgi 0.0078 0.0004 0.003 0.0049 0.0119 0.0043 0.0078 0.0006 0.0049 0.0066 0.0103 0.0037 0.0081 0.0004 0.0054 0.0007 0 0.0005
Lipid Particles 0.0054 0 0.0005 0.0002 0.0119 0.0004 0.0071 0.0001 0.0016 0.0105 0.0005 0 0.0011 0 0.0002 0.0005 0 0
Mitochondria 0.0103 0.0003 0.0012 0.0403 0.0139 0.0036 0.0043 0.0001 0.011 0.0012 0.0023 0.008 0.0234 0.0001 0.0182 0.0043 0 0.0002
None 0.0063 0.0003 0.0005 0.001 0.0013 0.001 0.0019 0.0002 0.0011 0.0013 0.0002 0.0005 0.003 0.0002 0.0002 0.0001 0.0001 0.0002
Nuclear Periphery 0.0074 0.0004 0.0023 0.0042 0.0052 0.0016 0.0069 0.0005 0.0012 0.0135 0.0006 0.0013 0.1482 0.0003 0.0006 0.008 0.0001 0.0012
Nucleolus 0.0005 0 0 0.0001 0.0009 0 0.0001 0 0.0019 0.0007 0 0 0.0004 0 0 0.0007 0 0
Nucleus 0.0023 0.0005 0.0012 0.0022 0.0021 0.0011 0.0018 0.0006 0.0029 0.0028 0.0006 0.001 0.0131 0.0004 0.0005 0.0022 0.0002 0.0015
Peroxisomes 0.0027 0 0.0002 0.0001 0.0035 0.0001 0.0108 0 0.0092 0.0108 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0
Punctate Nuclear 0.0057 0 0.0002 0.0002 0.0108 0.0002 0.005 0 0.0006 0.014 0.0001 0.0001 0.0006 0 0 0 0 0
Vacuole 0.0083 0.0013 0.0059 0.0245 0.0084 0.0043 0.0101 0.0019 0.0033 0.0179 0.0081 0.0026 0.0262 0.0007 0.0017 0.073 0.0005 0.0028
Vacuole Periphery 0.0018 0.0002 0.0014 0.0102 0.0037 0.0018 0.0023 0.0003 0.0009 0.0061 0.0028 0.0012 0.0375 0.0002 0.0005 0.1567 0.0001 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.9979 99.5262 104.8446 68.3201 96.0186 76.5001 73.2269 62.7645 65.2086 68.4924
Translational Efficiency 2.239 1.884 1.494 2.1903 1.6409 1.5721 1.7786 1.7079 1.4374 1.6674

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 515 2587 1533 2127 2084 212 1143 2210 2599 2799 2676

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1288.65 1653.02 2309.59 2580.76 1610.77 1859.88 2469.83 2516.36 1598.67 1818.89 2321.73 2553.25
Standard Deviation 260.78 396.64 377.18 480.93 268.85 325.42 332.43 475.41 275.44 350.55 376.37 479.64
Intensity Change Log 2 — 0.359244 0.841776 1.001936 — 0.207459 0.616661 0.643588 — 0.276898 0.721071 0.813918

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001111 0.000258 0.000096 0.000531 0.000085 0.000146 0.001069 0.000187 0.000123 0.000168 0.000170 0.000384
Bud Neck 0.002043 0.000445 0.000413 0.001535 0.000147 0.000319 0.010024 0.001601 0.000218 0.000344 0.001141 0.001563
Bud Site 0.112177* 0.009797 0.003778 0.013364 0.007226 0.007489 0.028692 0.012407 0.011167 0.007946 0.005665 0.012955
Cell Periphery 0.013805 0.001609 0.001121 0.007453 0.000451 0.000561 0.003498 0.001863 0.000953 0.000769 0.001301 0.005065
Cytoplasm 0.304064* 0.897062* 0.926425* 0.785708* 0.905758* 0.917037* 0.648684* 0.813441* 0.883161* 0.913079* 0.905389* 0.797554*
Cytoplasmic Foci 0.257566* 0.021354 0.004957 0.007225 0.039081 0.026051 0.040592 0.013533 0.047287 0.025120 0.007656 0.009919
Eisosomes 0.000029 0.000002 0.000001 0.000004 0.000001 0.000002 0.000007 0.000002 0.000002 0.000002 0.000001 0.000003
Endoplasmic Reticulum 0.034939 0.001971 0.000662 0.000543 0.004413 0.002116 0.001140 0.000328 0.005560 0.002087 0.000699 0.000451
Endosome 0.022373 0.002300 0.000731 0.002439 0.001946 0.002789 0.011271 0.003410 0.002713 0.002692 0.001530 0.002853
Golgi 0.067750 0.016594 0.007230 0.015454 0.013492 0.016416 0.035600 0.015285 0.015530 0.016451 0.009379 0.015382
Lipid Particles 0.008046 0.002707 0.003640 0.012183 0.001153 0.002021 0.022076 0.009645 0.001412 0.002157 0.005037 0.011099
Mitochondria 0.004168 0.001572 0.000107 0.000819 0.000150 0.000217 0.000039 0.001061 0.000301 0.000486 0.000102 0.000922
Mitotic Spindle 0.004397 0.001151 0.000676 0.002714 0.000717 0.002243 0.007819 0.004554 0.000855 0.002027 0.001217 0.003500
None 0.000552 0.001754 0.000493 0.000751 0.000310 0.000500 0.000353 0.000741 0.000319 0.000749 0.000482 0.000747
Nuclear Periphery 0.000308 0.000056 0.000044 0.000123 0.000146 0.000076 0.000331 0.000082 0.000152 0.000072 0.000065 0.000106
Nuclear Periphery Foci 0.003687 0.003415 0.002435 0.004145 0.001947 0.002486 0.017473 0.003659 0.002013 0.002670 0.003574 0.003938
Nucleolus 0.007966 0.000219 0.000042 0.000157 0.000017 0.000026 0.000079 0.000147 0.000315 0.000065 0.000044 0.000153
Nucleus 0.040554 0.006922 0.004671 0.007135 0.001253 0.002251 0.015324 0.008436 0.002729 0.003177 0.005478 0.007691
Peroxisomes 0.001953 0.000118 0.000043 0.000146 0.000059 0.000117 0.000208 0.000192 0.000130 0.000117 0.000055 0.000165
Vacuole 0.061378 0.020670 0.036280 0.123065 0.009170 0.010576 0.144993 0.100317 0.011131 0.012576 0.044514 0.113349
Vacuole Periphery 0.051136 0.010026 0.006156 0.014508 0.012477 0.006561 0.010728 0.009109 0.013929 0.007248 0.006502 0.012202

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.04 5.05 1.97 -1.22 -1.98 -4.76 -6.97 -7.39 -3.40 6.11 -2.28 -3.58 -2.25 -1.86 -1.65
Bud Neck 3.88 3.76 0.13 -10.35 -10.67 -4.52 -10.13 -12.37 -11.19 8.05 -3.41 -10.11 -17.74 -16.08 -5.14
Bud Site 6.25 6.62 6.02 -2.36 -8.53 -0.48 -6.36 -4.65 -4.46 4.55 3.16 5.18 -1.92 -5.97 -8.26
Cell Periphery 2.30 2.40 1.34 -6.22 -7.35 -1.62 -10.41 -8.00 -7.00 5.18 0.81 -1.58 -7.55 -8.64 -7.28
Cytoplasm -18.02 -19.31 -14.34 12.02 20.97 -2.10 14.10 14.38 15.55 -7.58 -5.33 -2.94 16.03 22.51 20.40
Cytoplasmic Foci 8.77 9.40 9.31 5.96 -4.76 6.03 -1.21 12.35 6.76 9.29 9.21 18.44 16.98 11.36 -3.90
Eisosomes 5.45 5.71 5.11 -2.13 -4.95 -1.97 -8.86 -6.82 -2.83 6.68 1.08 2.14 -3.07 -3.77 -4.99
Endoplasmic Reticulum 2.54 2.64 2.65 2.36 0.60 3.42 5.62 7.49 4.58 3.35 4.34 6.44 7.07 4.88 1.25
Endosome 4.73 5.10 4.71 -0.15 -4.42 -2.83 -7.07 -2.30 -0.61 5.78 0.03 4.40 0.03 0.00 -3.51
Golgi 5.51 6.61 5.82 1.17 -4.85 -2.30 -5.27 0.01 1.63 4.97 -0.79 7.08 1.41 1.93 -4.13
Lipid Particles 2.05 1.60 -1.62 -9.80 -8.92 -3.88 -11.18 -11.57 -10.31 5.83 -3.33 -11.01 -16.08 -14.65 -8.97
Mitochondria 1.20 2.35 1.94 0.61 -1.44 -0.83 6.73 -1.17 -1.04 -1.34 -0.66 2.79 -1.32 -0.66 -1.90
Mitotic Spindle 1.65 1.84 0.69 -2.40 -2.98 -3.40 -4.57 -2.68 -1.51 1.83 -3.15 -2.65 -3.76 -1.91 -2.96
None -2.31 0.22 -0.40 2.04 -0.81 -2.67 -0.97 -1.81 -0.82 -1.59 -4.09 -4.59 -2.16 0.27 -1.22
Nuclear Periphery 1.22 1.26 0.84 -3.92 -6.46 0.84 -2.84 0.65 -0.52 8.08 1.03 1.13 0.46 -1.38 -4.93
Nuclear Periphery Foci 0.25 0.97 -0.45 -1.58 -4.96 -0.98 -10.06 -5.15 -2.51 8.69 -1.40 -5.40 -6.67 -3.17 -1.42
Nucleolus 1.53 1.56 1.54 0.29 -11.95 -2.36 -12.85 -15.98 -13.58 -6.88 1.29 1.40 0.79 -2.95 -16.23
Nucleus 1.96 2.06 1.78 -1.57 -6.34 -4.54 -6.78 -13.73 -11.67 2.64 -0.71 -4.90 -9.57 -11.28 -6.93
Peroxisomes 2.53 2.64 2.50 -0.72 -6.79 -1.25 -4.39 -6.31 -1.52 0.82 0.26 2.48 -1.11 -1.17 -8.17
Vacuole 2.88 1.66 -4.95 -20.56 -19.32 -1.25 -11.74 -20.62 -20.33 2.40 -1.12 -16.24 -32.30 -32.02 -20.26
Vacuole Periphery 3.45 3.83 3.22 -1.36 -5.87 5.16 1.55 3.62 -1.31 0.81 5.49 6.73 2.38 -3.78 -5.34
Standard name
Human Ortholog
Description Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rbg1

Rbg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rbg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available