Standard name
Human Ortholog
Description Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.84 0.84 0.78 0.71 0.82 0.8 0.76 0.58 0.61 0.61 0.51 0.7 0.91 0.97 – 0.79 0.88 0.53 0.74 0.65 0.69 0.76 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.11 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.12 0.16 0.34 0.32 0.36 0.37 0.37 0.61 0.55 0.55 0.69 0 0 0 – 0 0 0.1 0 0.1 0.05 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.05
Vac/Vac Membrane 0.05 0.08 0 0 0.06 0 0 0 0 0 0 0.08 0.21 0.11 0 – 0.22 0.1 0.15 0.12 0.1 0.07 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 4 3 10 10
Bud 1 1 6 4 2 1 5 7 9 1 1 0 1 0 0 – 0 0 0 0 4 6 10 25
Bud Neck 0 0 1 4 1 3 3 2 2 3 0 0 0 0 0 – 0 0 0 1 0 3 5 11
Bud Site 1 0 0 0 0 0 0 2 1 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 2 1 0 0 0 1 0 1 1 – 1 2 0 0 2 0 2 4
Cytoplasm 47 159 211 175 230 378 297 386 214 168 49 26 276 586 527 – 146 70 82 166 175 284 410 353
Endoplasmic Reticulum 1 1 2 0 0 1 0 0 1 0 1 0 2 1 1 – 16 9 8 0 1 3 4 5
Endosome 2 0 0 0 4 0 0 2 0 0 0 0 19 14 2 – 2 0 4 1 2 2 9 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 0 0 2 3 4
Mitochondria 16 23 39 75 105 166 138 189 225 152 44 35 9 5 3 – 2 3 15 9 27 21 25 38
Nucleus 1 2 1 3 6 2 5 2 12 5 0 2 29 2 1 – 4 1 2 8 2 14 6 9
Nuclear Periphery 0 0 0 0 1 1 1 1 0 3 1 0 0 1 2 – 0 0 1 0 0 1 1 3
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 1
SpindlePole 0 0 1 5 2 1 3 5 1 1 1 0 0 0 0 – 1 0 6 4 9 24 18 30
Vac/Vac Membrane 3 15 8 5 19 10 4 11 7 7 1 4 83 73 20 – 40 8 22 27 26 28 17 52
Unique Cell Count 62 189 251 223 326 461 371 508 367 276 80 51 393 646 546 184 80 154 223 271 410 539 578
Labelled Cell Count 73 202 269 271 370 563 459 608 472 340 98 68 419 684 557 212 93 154 223 271 410 539 578


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.6 3.2 3.4 3.3 3.4 2.5 3.0 3.0 2.5 3.8 3.0 4.5 4.1 4.1 4.6 5.8 5.8 3.8 4.1 4.9
Std Deviation (1e-4) 0.6 0.5 0.9 1.4 1.0 2.7 1.0 1.0 1.4 0.9 2.9 1.7 1.0 1.1 1.1 1.4 1.6 1.4 1.0 1.4 1.7
Intensity Change (Log2) – – – 0.07 0.04 0.06 -0.37 -0.1 -0.12 -0.35 0.26 -0.09 0.48 0.36 0.36 0.52 0.84 0.85 0.23 0.34 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 6.1 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6931 3.2058 3.5156 2.9962 2.2795 2.5632 1.007 0.7629 1.0961 1.275 0.7051 0.752 2.0337 1.7703 1.5879 1.8676 1.8055 1.6372
Actin 0.0632 0.002 0.0134 0.0111 0.0043 0.0018 0.0226 0.0001 0.0046 0.0061 0.0018 0.009 0.0247 0.0179 0.0046 0.0058 0.0181 0.008
Bud 0.0013 0.001 0.0005 0.0009 0.0014 0.0001 0.0008 0 0.0001 0.0003 0.0001 0.0003 0.001 0.0077 0.0008 0.0008 0.0002 0.0004
Bud Neck 0.0012 0.0005 0.0015 0.002 0.0007 0.0013 0.0017 0.0001 0.0017 0.0003 0.0002 0.0016 0.0017 0.0009 0.0004 0.0011 0.0008 0.0008
Bud Periphery 0.0029 0.001 0.0004 0.0015 0.0026 0.0002 0.0022 0 0.0001 0.0006 0.0001 0.0006 0.0017 0.012 0.0006 0.0008 0.0002 0.0009
Bud Site 0.0038 0.0267 0.0051 0.0026 0.0004 0.0001 0.0039 0.0004 0.0064 0.0005 0.0001 0.0003 0.0073 0.0124 0.0102 0.0082 0.0005 0.0001
Cell Periphery 0.0005 0.0002 0.0002 0.0003 0.0001 0 0.0005 0.0001 0.0001 0.0001 0 0.0001 0.0007 0.0003 0.0001 0.0002 0.0001 0
Cytoplasm 0.1586 0.2833 0.1886 0.322 0.3062 0.3375 0.1155 0.1328 0.1211 0.2002 0.2041 0.1765 0.1698 0.2968 0.3146 0.3021 0.2531 0.3024
Cytoplasmic Foci 0.0295 0.0078 0.0111 0.0349 0.0317 0.0173 0.0346 0.0021 0.0283 0.0277 0.0097 0.0133 0.0257 0.0099 0.0076 0.0251 0.0074 0.0096
Eisosomes 0.0008 0.0003 0.0003 0.0004 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0138 0.0024 0.0028 0.0049 0.0036 0.0035 0.0116 0.001 0.0046 0.0047 0.0012 0.0023 0.0146 0.0023 0.0038 0.0073 0.0033 0.0022
Endosome 0.042 0.002 0.0038 0.0275 0.0053 0.0057 0.0636 0.0005 0.0167 0.081 0.0115 0.0069 0.0371 0.0021 0.0022 0.0065 0.0073 0.002
Golgi 0.0139 0.0004 0.0025 0.0168 0.0011 0.0011 0.0458 0 0.0061 0.041 0.0203 0.005 0.0166 0.0012 0.0009 0.0015 0.0149 0.0004
Lipid Particles 0.0226 0.0008 0.0091 0.008 0.0017 0.006 0.0284 0.0002 0.0147 0.0056 0.001 0.002 0.0383 0.0022 0.001 0.0016 0.0041 0.0006
Mitochondria 0.0455 0.0005 0.0015 0.0212 0.0011 0.0017 0.0134 0.0002 0.0018 0.0176 0.0425 0.0095 0.0214 0.0023 0.0004 0.0014 0.0203 0.0003
None 0.492 0.6254 0.5596 0.4206 0.5534 0.5136 0.5972 0.821 0.7256 0.5814 0.6819 0.6967 0.5172 0.5663 0.5864 0.4876 0.6138 0.6217
Nuclear Periphery 0.0181 0.0023 0.0059 0.0073 0.0038 0.0057 0.019 0.0011 0.0056 0.0046 0.0019 0.0037 0.0179 0.003 0.0039 0.0161 0.0036 0.0022
Nucleolus 0.002 0.0007 0.0045 0.0013 0.001 0.001 0.0027 0.0006 0.0004 0.0006 0.0002 0.0009 0.0024 0.0006 0.0004 0.001 0.0005 0.0002
Nucleus 0.0316 0.0256 0.1729 0.0913 0.0439 0.0638 0.0105 0.0355 0.0243 0.0179 0.0163 0.0606 0.0398 0.0326 0.0455 0.1093 0.0437 0.0388
Peroxisomes 0.0208 0.002 0.0026 0.0033 0.0059 0.007 0.0098 0 0.0334 0.0027 0.0008 0.0019 0.028 0.0102 0.0078 0.0063 0.0018 0.0014
Punctate Nuclear 0.0247 0.0138 0.0117 0.017 0.0303 0.0312 0.0061 0.0036 0.003 0.0034 0.0044 0.007 0.0151 0.0175 0.0077 0.015 0.0042 0.0073
Vacuole 0.007 0.0011 0.0015 0.0039 0.001 0.001 0.0067 0.0006 0.0009 0.0024 0.0011 0.0014 0.0115 0.0014 0.0008 0.0016 0.0013 0.0005
Vacuole Periphery 0.0043 0.0001 0.0003 0.0015 0.0003 0.0003 0.003 0 0.0004 0.0009 0.0005 0.0003 0.0072 0.0002 0.0002 0.0005 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9505 40.79 29.8093 40.5593 38.5441 44.9133 48.8976 54.6177 49.865 45.6876
Translational Efficiency 1.0476 1.4186 1.2952 0.9715 0.843 1.1567 1.2325 1.1779 1.1733 1.0598

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
192 1537 1197 1798 533 1701 247 2275 725 3238 1444 4073

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.34 695.06 832.44 902.08 663.87 676.50 894.32 932.15 670.35 685.31 843.02 918.88
Standard Deviation 71.56 111.17 109.07 128.42 69.10 85.20 92.75 134.41 70.59 98.82 108.98 132.65
Intensity Change Log 2 — 0.014016 0.274225 0.390134 — 0.027189 0.429890 0.489661 — 0.020499 0.352748 0.439855

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000264 0.000306 0.001451 0.000061 0.000268 0.000259 0.000644 0.000056 0.000266 0.000298 0.001000
Bud Neck 0.000807 0.015261 0.003333 0.019844 0.002650 0.026555 0.001382 0.018261 0.002162 0.021194 0.002999 0.018960
Bud Site 0.000489 0.005453 0.006082 0.056169 0.000442 0.007389 0.002495 0.050389 0.000454 0.006470 0.005468 0.052940
Cell Periphery 0.000161 0.000128 0.000088 0.000178 0.000145 0.000118 0.000216 0.000184 0.000149 0.000123 0.000110 0.000181
Cytoplasm 0.261399* 0.396865* 0.148237* 0.369335* 0.257027* 0.374181* 0.189164* 0.472299* 0.258185* 0.384949* 0.155238* 0.426846*
Cytoplasmic Foci 0.068613 0.140844 0.002917 0.010827 0.110485 0.176117 0.019724 0.010854 0.099396 0.159374 0.005792 0.010842
Eisosomes 0.000077 0.000089 0.000028 0.000038 0.000093 0.000111 0.000109 0.000028 0.000089 0.000101 0.000042 0.000032
Endoplasmic Reticulum 0.000372 0.001385 0.004027 0.002427 0.000912 0.000902 0.003229 0.001777 0.000769 0.001131 0.003890 0.002064
Endosome 0.001772 0.007821 0.001187 0.010341 0.002183 0.008831 0.002429 0.011865 0.002074 0.008352 0.001399 0.011192
Golgi 0.000317 0.002004 0.000138 0.003335 0.000241 0.001971 0.000081 0.007538 0.000261 0.001987 0.000128 0.005683
Lipid Particles 0.005708 0.008989 0.000899 0.001508 0.011312 0.010251 0.003190 0.000657 0.009828 0.009652 0.001291 0.001033
Mitochondria 0.005915 0.007109 0.001459 0.008880 0.004099 0.014862 0.000328 0.008848 0.004580 0.011182 0.001265 0.008862
Mitotic Spindle 0.000572 0.004796 0.002339 0.095291 0.002080 0.003714 0.001876 0.090432 0.001681 0.004228 0.002260 0.092577
None 0.005565 0.012468 0.005561 0.011949 0.006479 0.006186 0.007250 0.010844 0.006237 0.009168 0.005850 0.011332
Nuclear Periphery 0.000714 0.001171 0.001918 0.002202 0.001413 0.000983 0.001301 0.002299 0.001228 0.001072 0.001812 0.002257
Nuclear Periphery Foci 0.000185 0.000930 0.000552 0.001033 0.000518 0.000566 0.000966 0.001087 0.000430 0.000739 0.000623 0.001063
Nucleolus 0.002690 0.004060 0.000716 0.001795 0.003453 0.004359 0.001408 0.001023 0.003251 0.004217 0.000834 0.001364
Nucleus 0.634869* 0.345314* 0.800284* 0.325366* 0.583046* 0.314206* 0.699787* 0.233937* 0.596770* 0.328972* 0.783094* 0.274298*
Peroxisomes 0.001220 0.009573 0.000469 0.003218 0.002504 0.014987 0.000469 0.002278 0.002164 0.012417 0.000469 0.002693
Vacuole 0.006804 0.033734 0.019278 0.070550 0.010383 0.032387 0.064179 0.069928 0.009435 0.033027 0.026958 0.070202
Vacuole Periphery 0.001710 0.001741 0.000183 0.004262 0.000472 0.001058 0.000159 0.004830 0.000800 0.001382 0.000179 0.004579

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.45 -8.62 -4.58 -3.34 -2.90 -6.02 -6.21 -10.88 -4.01 0.18 -8.11 -10.89 -6.86 -4.33 -3.07
Bud Neck -12.14 -6.32 -12.50 1.53 -8.16 -12.04 1.08 -9.61 6.91 -11.30 -15.66 -2.82 -14.02 6.61 -11.46
Bud Site -6.97 -3.61 -9.42 -6.84 -6.16 -6.21 -2.92 -11.90 -6.29 -8.91 -9.09 -4.08 -15.06 -9.36 -8.82
Cell Periphery 1.31 2.70 1.15 -0.49 -4.88 2.69 -3.88 3.66 1.43 6.56 2.72 2.88 3.25 0.85 0.31
Cytoplasm -6.87 6.73 0.41 15.72 -15.25 -10.13 5.59 -7.68 3.65 -12.28 -13.06 12.08 -4.79 12.76 -25.28
Cytoplasmic Foci -7.93 8.74 8.45 30.18 -4.14 -7.70 14.09 16.82 35.00 5.05 -9.19 18.44 18.60 46.02 0.92
Eisosomes -1.33 5.84 3.79 6.10 -5.84 -1.97 -3.19 11.46 16.91 9.87 -1.55 7.66 11.27 15.47 3.94
Endoplasmic Reticulum -7.20 -17.67 -19.94 -14.24 4.09 -0.26 -8.78 -9.83 -10.64 5.64 -4.12 -18.63 -19.38 -17.30 8.50
Endosome -7.40 0.39 -2.22 6.16 -4.03 -8.89 -2.08 -1.50 6.52 0.09 -12.06 1.41 -2.60 8.88 -3.85
Golgi -4.00 1.26 -1.99 2.49 -3.11 -5.48 5.13 -2.93 0.40 -3.26 -6.76 2.51 -3.56 1.66 -4.10
Lipid Particles -2.52 4.82 4.67 10.69 -0.96 1.05 5.69 7.97 11.98 7.66 0.56 8.20 8.76 16.08 5.05
Mitochondria -0.30 1.13 0.59 3.83 -3.16 -4.67 2.14 0.26 6.99 -6.48 -3.52 2.03 0.59 7.96 -5.09
Mitotic Spindle -3.51 -2.08 -12.63 -10.97 -11.70 -0.87 -0.62 -13.25 -14.02 -12.54 -1.74 -0.70 -17.27 -17.74 -17.97
None -4.01 1.12 -3.37 1.81 -4.66 0.45 0.46 -5.35 -6.75 -4.76 -2.60 1.71 -5.53 -1.87 -8.33
Nuclear Periphery -3.68 -10.86 -19.97 -17.99 -7.20 2.80 -2.66 -9.96 -14.08 -7.86 1.23 -7.81 -16.60 -21.56 -7.93
Nuclear Periphery Foci -5.79 -7.28 -8.45 1.03 -0.49 -0.70 -2.11 -0.81 0.08 1.99 -3.74 -4.15 -4.09 0.90 2.07
Nucleolus -1.73 3.12 2.75 7.20 -1.63 -0.55 4.23 4.97 7.52 1.63 -1.04 5.76 5.58 10.41 -0.95
Nucleus 12.75 -7.28 6.40 -14.24 31.69 19.49 -3.98 11.09 -12.91 12.31 22.98 -14.77 11.98 -18.91 40.28
Peroxisomes -8.60 2.40 0.94 9.31 -2.01 -10.12 5.74 5.30 12.70 -0.49 -12.52 6.36 5.21 15.73 -1.96
Vacuole -10.97 -8.79 -25.48 -20.06 -19.34 -11.56 -9.23 -27.05 -19.35 0.19 -16.33 -12.54 -37.20 -27.82 -18.43
Vacuole Periphery -0.00 1.06 0.39 1.17 -3.83 -3.15 2.00 -2.19 -0.44 -3.46 -1.27 1.56 -0.84 0.69 -4.98
Standard name
Human Ortholog
Description Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (55%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rpn4

Rpn4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available