Standard name
Description Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.07 0.07 0.09 0.13 0.09 0.07 0.15 0.11 0.08 0.05 0 0 0 0.05 0.12 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.12 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.22 0.06 0.05 0.07 0.11 0.13 0.18 0.19 0.33 0.22 0.28 0.07 0.09 0.11 0.13 0.19 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.54 0.63 0.51 0.61 0.62 0.4 0.47 0.24 0.29 0.17 0.5 0.42 0.45 0 0 0 0.11 0.74 0.83 0.74 0.75 0.73
Endosome 0 0 0 0.08 0.08 0.07 0.12 0.12 0.17 0.2 0.22 0 0 0 0 0 0 0.2 0.05 0 0.05 0.05 0.05
Golgi 0.06 0.23 0.3 0.39 0.1 0 0 0 0 0 0 0.35 0.38 0.27 0.7 0.61 0.67 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.07 0.06 0
Nucleolus 0.61 0.15 0.18 0.18 0.08 0.08 0.07 0.08 0.09 0.11 0.11 0.17 0.16 0.13 0.07 0.05 0.07 0.1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0
Vac/Vac Membrane 0.11 0.14 0.07 0.08 0.15 0.1 0.23 0.16 0.24 0.15 0.21 0 0.07 0.09 0.16 0.16 0.15 0 0.06 0 0.05 0.09 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 17 11 11 14 17 36 30 28 45 35 32 22 18 3 8 9 8 12 5 8 5 6
Bud 0 1 1 1 0 2 11 3 9 21 11 6 1 0 0 0 0 1 2 0 4 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 1 0 1 1 2 7 9 2 5 6 26 39 55 10 11 5 0 0 0 0 0 0
Cytoplasm 4 25 11 10 22 25 50 65 125 65 91 26 40 51 40 36 31 0 3 0 1 1 2
Endoplasmic Reticulum 0 209 150 75 119 120 109 156 91 88 56 199 190 217 11 2 4 8 369 251 253 355 452
Endosome 0 2 5 12 16 13 34 40 66 60 70 5 13 8 2 1 1 15 27 8 17 23 30
Golgi 1 91 72 58 20 4 5 5 11 6 3 138 173 130 214 116 125 1 4 1 7 7 12
Mitochondria 0 0 0 0 1 1 2 2 1 6 9 0 0 1 10 2 3 2 2 1 2 3 6
Nucleus 2 1 0 0 0 0 0 0 2 0 3 0 1 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 4 3 2 4 2 5 2 1 0 1 1 1 0 1 0 3 35 18 22 29 19
Nucleolus 11 59 44 26 16 16 20 26 34 32 37 69 72 62 22 9 14 7 1 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0
SpindlePole 0 2 2 0 0 0 4 1 4 1 1 4 7 16 4 0 2 7 5 1 1 0 4
Vac/Vac Membrane 2 55 16 12 30 20 63 52 90 45 69 12 31 41 48 30 28 1 29 8 18 42 64
Unique Cell Count 18 389 239 148 196 195 274 335 382 302 323 396 455 478 307 191 187 74 502 303 342 476 617
Labelled Cell Count 20 463 316 209 241 224 343 394 465 375 392 518 590 601 364 217 222 74 502 303 342 476 617


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 29.7 24.0 22.4 19.5 16.3 15.4 14.8 16.1 13.8 14.7 26.3 25.1 23.9 28.2 28.4 28.7 30.7 30.5 30.9
Std Deviation (1e-4) 5.1 5.8 4.3 4.1 3.1 2.8 2.8 2.7 7.2 2.7 5.5 5.0 5.2 4.8 5.9 6.2 6.5 9.5 6.4 6.2
Intensity Change (Log2) -0.1 -0.3 -0.56 -0.64 -0.7 -0.58 -0.8 -0.71 0.13 0.06 -0.01 0.23 0.24 0.26 0.35 0.34 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 1.1 1.7 3.3 2.0 1.4 3.9 2.7 1.7 0.1 -0.5 -2.7 -0.2 0.1
Bud 0 0 0 2.7 0 0 3.8 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 4.0 4.7 5.5 0 0 0
Cytoplasm 0.9 2.6 3.1 4.8 5.2 8.2 5.6 7.2 1.0 2.0 2.7 3.4 4.7 4.1
Endoplasmic Reticulum -2.3 -0.4 -0.3 -5.2 -3.8 -9.7 -7.8 -11.0 -3.1 -5.3 -4.4 -15.0 -13.3 -12.9
Endosome 2.8 2.9 2.4 4.4 4.3 5.8 6.3 6.7 0 0.6 0 0 0 0
Golgi 1.8 -5.1 -7.7 -9.0 -9.9 -9.7 -9.3 -10.1 1.2 2.1 -0.8 9.2 6.4 7.5
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 -3.1 -3.1 -3.8 -3.8 -3.5 -2.6 -2.3 -0.3 -0.9 -1.9 -4.0 -4.3 -3.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0
Vacuole 0.5 2.9 1.3 5.1 3.2 5.4 3.0 4.8 -2.2 0.1 0.9 3.2 3.0 2.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tsc13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available