Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.53 0.6 0.48 0.48 0.41 0.42 0.41 0.32 0.41 0.3 0.33 0.52 0.57 0.67 – 0.51 0.51 0.33 0.36 0.33 0.39 0.51 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.34 0.18 0.33 0.44 0.29 0.59 0.66 0.64 0.77 0.75 0.83 0.79 0.06 0.05 0 – 0 0.06 0.12 0 0.1 0.05 0.05 0.07
Nucleus 0.06 0.1 0.11 0.09 0.1 0.11 0.07 0.11 0.08 0.08 0.08 0.08 0.16 0.1 0 – 0.21 0.24 0.13 0.22 0.19 0.21 0.12 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0.23 0.38 0.16 0.13 0.28 0.11 0.06 0.08 0.07 0.05 0.06 0.06 0.35 0.38 0.36 – 0.33 0.28 0.26 0.32 0.27 0.23 0.19 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 1 0 0 0 2 4
Bud 0 0 3 1 0 2 2 1 4 4 6 8 0 0 0 – 0 0 3 1 2 3 8 16
Bud Neck 0 0 0 1 0 0 2 3 0 0 0 1 0 0 0 – 0 0 0 0 0 2 4 6
Bud Site 1 0 0 0 0 0 3 1 3 2 2 7 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 – 1 0 0 0 0 0 1 0
Cytoplasm 65 89 67 61 92 98 155 144 70 143 77 145 142 251 368 – 117 83 53 72 48 95 182 119
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 1 0 0 0 0 1 1 – 10 8 3 0 1 0 3 5
Endosome 0 0 0 2 7 1 0 1 0 0 0 0 6 1 16 – 5 6 3 2 2 1 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 1 0 0 1 4
Mitochondria 37 30 37 56 56 143 242 223 170 265 209 341 17 20 12 – 9 9 19 7 14 12 18 24
Nucleus 6 17 12 12 19 27 25 39 17 27 21 34 44 42 20 – 48 39 20 43 27 49 42 19
Nuclear Periphery 1 1 0 1 0 2 0 0 1 2 0 0 0 0 0 – 0 0 1 0 0 0 1 0
Nucleolus 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 3 2 0 1 3 0 1 0 – 4 2 1 1 2 11 12 7
Vac/Vac Membrane 25 64 18 16 54 27 22 29 16 17 15 26 97 164 200 – 77 45 43 63 40 55 66 104
Unique Cell Count 109 167 111 127 191 241 365 349 221 353 253 434 275 437 551 231 162 163 199 149 242 357 328
Labelled Cell Count 136 202 138 150 229 301 454 444 284 460 332 565 306 480 617 272 193 163 199 149 242 357 328


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.9 3.8 3.7 3.9 3.2 2.6 3.2 2.9 2.6 2.7 2.6 4.4 4.2 4.2 4.1 5.1 5.4 4.2 4.4 5.0
Std Deviation (1e-4) 0.9 0.9 1.6 1.3 2.0 1.3 1.0 1.5 1.2 1.4 0.9 1.3 1.3 1.0 1.2 1.0 1.1 1.0 1.5 1.5 1.8
Intensity Change (Log2) – – – -0.05 0.05 -0.22 -0.54 -0.26 -0.37 -0.52 -0.46 -0.51 0.23 0.15 0.15 0.12 0.43 0.52 0.16 0.23 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.3 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1804 0.008 0.0456 -0.0808 -0.1964 -0.1834 -0.0493 0.2583 0.1822 -0.381 0.2768 -0.025 1.6932 1.9835 1.9881 1.9357 1.6577 1.6286
Actin 0.0189 0.0008 0.0137 0.053 0.0213 0.0082 0.0159 0.0004 0.0092 0.0004 0.0571 0.0033 0.0289 0.0008 0.0087 0.0025 0.0087 0.0007
Bud 0.0005 0.0002 0.0002 0.0003 0.0001 0.0013 0.0006 0 0.0001 0 0.0004 0.0003 0.0073 0.0026 0.0038 0.0059 0.0026 0.0016
Bud Neck 0.0054 0.0007 0.0037 0.0011 0.0004 0.0067 0.0034 0.0003 0.0004 0.0005 0.0007 0.0033 0.0061 0.0007 0.0006 0.0013 0.0007 0.0072
Bud Periphery 0.0011 0.0001 0.0002 0.0004 0.0001 0.0005 0.0011 0 0.0002 0 0.0006 0.0007 0.0022 0.0006 0.0015 0.0056 0.0108 0.0022
Bud Site 0.0032 0.003 0.0011 0.0006 0.0002 0.0003 0.0046 0.0005 0.001 0.0001 0.0009 0.0004 0.0037 0.0021 0.0057 0.0025 0.0016 0.0005
Cell Periphery 0.0006 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0002
Cytoplasm 0.1799 0.1893 0.1424 0.1127 0.1236 0.126 0.141 0.1983 0.1344 0.0846 0.1878 0.135 0.1684 0.1725 0.1325 0.0941 0.1302 0.1843
Cytoplasmic Foci 0.0129 0.0078 0.0124 0.0029 0.0138 0.0061 0.0361 0.004 0.0121 0.0024 0.0108 0.0053 0.0111 0.0031 0.0097 0.0123 0.0051 0.0061
Eisosomes 0.0007 0.0001 0.0002 0.0002 0.0003 0.0002 0.0007 0.0001 0.0002 0.0001 0.0003 0.0001 0.0018 0.0002 0.0004 0.0005 0.0002 0.0001
Endoplasmic Reticulum 0.0077 0.0072 0.0035 0.002 0.0015 0.0038 0.011 0.0057 0.0045 0.0017 0.0018 0.0022 0.0041 0.0029 0.0063 0.0013 0.0026 0.0025
Endosome 0.0157 0.0036 0.008 0.0032 0.0042 0.0058 0.0361 0.0037 0.0116 0.0024 0.005 0.0033 0.0076 0.003 0.0069 0.0017 0.004 0.0036
Golgi 0.0036 0.0002 0.003 0.0019 0.0022 0.0027 0.016 0.0001 0.0028 0.0001 0.0049 0.0035 0.0035 0.0001 0.0016 0.0006 0.0009 0.0001
Lipid Particles 0.0157 0.002 0.0043 0.0008 0.0063 0.005 0.0291 0.0005 0.0036 0.0004 0.0404 0.0011 0.0097 0.0003 0.003 0.0065 0.0007 0.0005
Mitochondria 0.0056 0.0006 0.0026 0.0012 0.001 0.0013 0.0131 0.0005 0.0013 0.0013 0.0044 0.0028 0.0046 0.001 0.003 0.0127 0.0113 0.0014
None 0.3712 0.338 0.3719 0.2374 0.4843 0.244 0.473 0.5587 0.4789 0.3801 0.5373 0.4691 0.3957 0.4155 0.4133 0.3741 0.4461 0.3148
Nuclear Periphery 0.0249 0.0235 0.0147 0.0123 0.0081 0.0088 0.0176 0.0119 0.0096 0.0173 0.0041 0.0065 0.0162 0.0093 0.0125 0.0098 0.0183 0.0104
Nucleolus 0.0055 0.0041 0.0037 0.0047 0.0021 0.0094 0.0043 0.002 0.002 0.0063 0.0012 0.0054 0.0044 0.0033 0.0025 0.0082 0.0032 0.0069
Nucleus 0.2742 0.3567 0.388 0.5332 0.3041 0.5076 0.1359 0.1965 0.3073 0.4233 0.1134 0.3357 0.2858 0.3628 0.3587 0.3697 0.309 0.4184
Peroxisomes 0.0125 0.0004 0.0072 0.0004 0.015 0.0023 0.0233 0.0001 0.006 0.0001 0.0198 0.001 0.0144 0.0002 0.0032 0.0134 0.0005 0.0004
Punctate Nuclear 0.0347 0.059 0.0171 0.0296 0.0104 0.0573 0.0276 0.0139 0.013 0.0775 0.0072 0.0196 0.021 0.0167 0.0236 0.0751 0.0416 0.0351
Vacuole 0.0044 0.0021 0.0017 0.0017 0.0007 0.0023 0.0067 0.0021 0.0015 0.0008 0.0012 0.0012 0.0023 0.0019 0.0017 0.0013 0.0013 0.0026
Vacuole Periphery 0.001 0.0004 0.0005 0.0003 0.0002 0.0004 0.0025 0.0003 0.0003 0.0004 0.0003 0.0003 0.0007 0.0003 0.0004 0.0005 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3827 3.5661 8.4678 10.3217 8.2754 8.7713 7.2113 5.5972 9.2732 10.8163
Translational Efficiency 0.7285 0.7498 0.435 0.3773 0.443 0.5785 0.5853 0.6318 0.4732 0.4874

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1484 1349 200 1273 1454 1366 578 940 2938 2715 778 2213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.58 698.45 852.72 797.41 703.59 698.75 936.65 847.77 659.64 698.60 915.07 818.80
Standard Deviation 66.54 94.46 101.17 101.14 83.08 89.39 107.92 211.60 86.86 91.94 112.38 159.76
Intensity Change Log 2 — 0.179869 0.467784 0.371034 — -0.009959 0.412775 0.268938 — 0.081815 0.438728 0.317522

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000346 0.000646 0.001127 0.000028 0.000568 0.000559 0.003181 0.000020 0.000458 0.000582 0.001999
Bud Neck 0.001794 0.019920 0.001743 0.014323 0.001253 0.024546 0.004237 0.018546 0.001526 0.022248 0.003596 0.016117
Bud Site 0.000335 0.007708 0.001430 0.021394 0.000420 0.009954 0.004378 0.144649* 0.000377 0.008838 0.003620 0.073748
Cell Periphery 0.000168 0.000189 0.000171 0.000175 0.000118 0.000214 0.000122 0.006868 0.000143 0.000201 0.000134 0.003018
Cytoplasm 0.377375* 0.293156* 0.107682* 0.258744* 0.454573* 0.298656* 0.115023* 0.373188* 0.415579* 0.295923* 0.113136* 0.307356*
Cytoplasmic Foci 0.046193 0.150818 0.005360 0.011582 0.046953 0.151203 0.001722 0.015437 0.046569 0.151012 0.002658 0.013219
Eisosomes 0.000053 0.000104 0.000058 0.000038 0.000036 0.000111 0.000036 0.000075 0.000045 0.000108 0.000041 0.000054
Endoplasmic Reticulum 0.000266 0.001987 0.008180 0.003874 0.000473 0.001004 0.006934 0.001523 0.000368 0.001493 0.007254 0.002876
Endosome 0.000882 0.008247 0.001622 0.005729 0.001150 0.008462 0.001547 0.003899 0.001015 0.008355 0.001566 0.004952
Golgi 0.000291 0.004190 0.000091 0.004605 0.000318 0.002679 0.000086 0.002401 0.000304 0.003430 0.000087 0.003669
Lipid Particles 0.002080 0.009854 0.001685 0.000994 0.001893 0.007528 0.000790 0.000741 0.001988 0.008684 0.001020 0.000886
Mitochondria 0.001499 0.015807 0.000406 0.002884 0.000776 0.015625 0.001746 0.007758 0.001141 0.015715 0.001401 0.004954
Mitotic Spindle 0.000666 0.003097 0.000674 0.037865 0.001608 0.006350 0.002549 0.022782 0.001132 0.004734 0.002067 0.031458
None 0.015622 0.012353 0.005404 0.013972 0.012282 0.008402 0.004703 0.026104 0.013969 0.010365 0.004884 0.019125
Nuclear Periphery 0.000419 0.001061 0.001048 0.003096 0.000360 0.000873 0.001754 0.000817 0.000389 0.000966 0.001572 0.002128
Nuclear Periphery Foci 0.000486 0.000931 0.001551 0.000858 0.000286 0.000452 0.000921 0.000705 0.000387 0.000690 0.001083 0.000793
Nucleolus 0.001739 0.004732 0.001777 0.001657 0.001114 0.004581 0.000506 0.001993 0.001430 0.004656 0.000832 0.001800
Nucleus 0.545380* 0.421853* 0.835813* 0.557700* 0.469310* 0.412517* 0.824769* 0.331306* 0.507733* 0.417156* 0.827608* 0.461536*
Peroxisomes 0.000676 0.018214 0.000407 0.000899 0.000659 0.016889 0.000209 0.000959 0.000668 0.017547 0.000260 0.000924
Vacuole 0.003920 0.024209 0.024098 0.054774 0.006128 0.028065 0.027196 0.032970 0.005013 0.026149 0.026400 0.045513
Vacuole Periphery 0.000143 0.001226 0.000155 0.003712 0.000262 0.001320 0.000214 0.004096 0.000202 0.001273 0.000199 0.003875

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.09 -8.89 -5.35 -2.91 0.35 -2.73 -9.17 -10.92 -7.07 -8.22 -4.41 -12.15 -11.79 -7.03 -6.39
Bud Neck -10.45 -0.68 -9.96 2.21 -10.15 -11.66 -7.13 -13.62 2.01 -9.78 -15.64 -6.51 -16.23 3.18 -12.20
Bud Site -7.08 -6.04 -8.72 -3.94 -7.63 -7.33 -3.42 -19.48 -17.74 -18.66 -10.14 -3.98 -19.76 -16.64 -18.15
Cell Periphery -1.50 -1.53 2.17 3.19 2.68 -3.55 -1.02 -8.51 -8.34 -8.49 -3.80 -0.48 -8.28 -8.05 -8.26
Cytoplasm 9.39 19.04 19.84 9.92 -6.29 16.17 38.07 9.88 -4.73 -24.22 18.02 42.59 21.54 4.49 -20.87
Cytoplasmic Foci -18.51 15.71 15.16 27.05 -2.15 -18.80 19.90 12.63 26.26 -9.46 -26.39 26.35 20.01 37.91 -10.21
Eisosomes -10.20 -2.73 -3.17 7.48 1.40 -14.77 -4.06 -9.83 4.43 -5.76 -17.58 -3.75 -9.32 8.65 -3.27
Endoplasmic Reticulum -8.74 -9.74 -19.96 -12.10 3.98 -4.46 -15.13 -10.16 -7.55 11.53 -9.77 -18.15 -21.78 -14.56 9.58
Endosome -9.86 -3.72 -5.65 5.93 -2.24 -8.77 -2.85 -2.08 5.15 -1.24 -13.12 -4.45 -5.17 7.81 -2.89
Golgi -5.44 1.63 -1.76 2.62 -2.00 -4.17 2.12 -2.68 1.47 -3.28 -6.85 2.80 -2.74 2.89 -3.25
Lipid Particles -11.80 -0.48 2.57 13.68 2.47 -10.20 5.57 5.64 11.99 0.45 -15.56 3.47 5.02 18.11 1.14
Mitochondria -7.52 1.72 -0.71 7.63 -6.31 -8.13 -0.97 -8.03 4.56 -6.03 -11.05 0.03 -6.03 8.88 -5.44
Mitotic Spindle -2.97 -0.50 -10.10 -9.48 -10.03 -2.97 -0.94 -7.90 -6.15 -7.65 -4.01 -1.48 -12.65 -11.12 -12.21
None 1.75 6.71 3.56 1.63 -3.08 3.00 8.92 -3.99 -5.97 -10.05 3.18 11.16 0.53 -2.44 -9.20
Nuclear Periphery -4.67 -6.03 -15.68 -13.59 -11.63 -7.42 -17.46 -8.58 -4.78 4.28 -7.49 -16.53 -17.71 -14.26 -6.33
Nuclear Periphery Foci -1.69 -3.42 -3.99 -2.78 1.12 -2.26 -5.92 -2.39 -1.94 0.19 -2.19 -5.67 -4.72 -3.52 0.29
Nucleolus -5.68 -0.74 -2.66 1.21 -0.23 -6.26 6.64 -3.35 3.53 -4.93 -8.39 0.19 -3.68 2.68 -2.63
Nucleus 11.48 -14.26 -2.13 -12.29 12.35 5.39 -28.04 9.45 4.28 34.04 11.94 -30.60 3.23 -7.25 30.40
Peroxisomes -11.67 0.39 0.21 11.78 -0.26 -11.52 2.99 0.05 11.52 -2.80 -16.39 2.73 0.21 16.48 -3.10
Vacuole -15.23 -6.00 -20.99 -16.45 -7.47 -12.96 -9.88 -12.87 -6.31 0.81 -19.57 -11.64 -24.15 -17.03 -5.51
Vacuole Periphery -9.35 -1.12 -2.63 -0.20 -2.54 -4.65 0.71 -5.29 -2.91 -5.60 -8.41 0.33 -5.29 -1.95 -5.41
Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
Localization
Cell Percentages cytoplasm (24%), nucleus (8%), mixed (63%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad57

Rad57


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad57-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available