Standard name
Human Ortholog
Description T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.21 0.12 0.07 0.05 0.05 0.05 0.06 0.08
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0.16 0.31 0.47 0.41 0.35 0.35 0.06 0.08 0.06 0.12 0.18 0.21
Mitochondria 0.95 0.9 0.95 0.95 0.94 0.92 0.95 0.88 0.92 0.93 0.94 0.94 0.91 0.77 0.73 0 0.06 0.12 0.78 0.79 0.79 0.69 0.65 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.11 0.07 0.06 0.11 0.16 0.12 0.11 0.1 0.13 0.13 0.13 0.08 0.06 0 0.19 0.27 0.18 0 0 0 0.06 0 0
Peroxisomes 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.11 0.09 0.06 0.29 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 5 5 3 0 1 2 3 0 0 2 2 5 10 12 10 2 6 10 13 5 17 21
Bud 0 0 2 1 3 1 6 6 2 5 3 1 0 0 1 3 1 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3 2 2 0 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 6 2 1 1 0 2 2 2 1 3 3 6 4 5 1 1 0 0 0 0 0 0
Cytoplasm 0 0 0 6 6 0 3 6 4 4 4 3 0 4 5 5 3 2 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 2 0 1 0 1 0 1 0 2 5 1 0 0 0 2 0 0 0 2
Endosome 0 0 1 0 0 0 0 1 0 0 1 0 0 0 1 1 1 0 0 0 1 1 1 3
Golgi 0 15 8 3 9 1 0 3 5 4 3 1 43 98 189 32 17 6 5 19 15 13 46 55
Mitochondria 87 198 238 191 249 273 461 381 347 371 379 395 238 248 291 2 3 2 72 188 207 78 170 143
Nucleus 1 0 1 1 1 0 1 0 1 2 0 1 1 0 0 0 1 0 0 0 0 0 0 1
Nuclear Periphery 1 0 4 3 2 14 19 13 12 15 5 20 2 1 1 3 1 0 0 1 1 0 0 1
Nucleolus 14 25 18 12 29 48 56 49 36 52 51 55 21 19 10 15 13 3 1 2 0 7 6 6
Peroxisomes 2 2 5 2 2 0 5 20 5 2 2 3 3 5 6 1 2 0 2 5 20 1 2 4
SpindlePole 0 0 1 0 0 0 2 2 3 1 1 2 0 0 0 0 2 0 0 0 0 0 1 1
Vac/Vac Membrane 4 23 5 0 5 7 10 14 9 11 14 16 20 42 44 7 3 5 2 4 0 2 11 14
Unique Cell Count 92 219 251 201 265 296 485 435 376 401 402 422 261 321 400 79 48 17 93 239 262 114 264 259
Labelled Cell Count 109 266 294 226 310 347 567 502 431 470 464 506 333 430 567 87 58 21 93 239 262 114 264 259


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.5 36.6 31.1 33.6 29.0 24.8 22.4 23.2 22.0 21.5 20.2 20.5 46.7 46.4 45.5 30.8 30.4 28.8 32.2 37.9 41.7
Std Deviation (1e-4) 6.8 10.6 9.2 14.4 8.2 7.8 7.3 7.3 7.2 7.5 7.7 7.4 13.2 14.3 14.2 10.2 10.8 12.2 10.5 13.8 13.4
Intensity Change (Log2) – – – 0.11 -0.1 -0.32 -0.47 -0.42 -0.5 -0.53 -0.62 -0.6 0.59 0.58 0.55 -0.01 -0.03 -0.11 0.05 0.29 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.4 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 -0.4 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.1 0 0 0 -1.6 0 0 0 5.0 8.4 11.9 8.9 0 0
Mitochondria 0.1 -0.4 -1.2 0.1 -3.1 -1.2 -1.2 -0.3 -0.6 -1.6 -5.8 -7.0 -16.1 -14.2 -10.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.0 1.7 1.1 1.2 1.6 0 2.1 0 0 0 0 0 0
Nucleolus -0.5 1.5 3.2 1.9 1.7 1.1 2.3 2.2 2.4 0.4 -0.6 -2.9 3.1 0 0
Peroxisomes 0 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0.3 0.1 0.9 0.3 0.6 1.1 1.3 3.0 4.8 4.2 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.0049 69.7626 66.1845 73.344 62.2332 68.9836 53.5395 66.6018 62.2267 52.4917 58.5311 62.8983 69.7279 65.5796 64.4793 59.7327 60.7465 62.8346
Actin 0.0154 0.0026 0.0006 0.0017 0.0167 0.0003 0.0144 0.0009 0.002 0.0005 0.0004 0 0.0056 0.0009 0.0015 0.001 0.0001 0.0001
Bud 0.0001 0 0 0.0002 0.0004 0 0.0002 0 0 0 0 0 0 0.0016 0 0 0 0
Bud Neck 0.0002 0 0 0.0001 0.0005 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0 0
Bud Periphery 0.0002 0 0.0001 0.0003 0.0003 0 0.0005 0 0 0.0001 0 0 0 0.0009 0.0001 0 0.0001 0
Bud Site 0.0004 0.0001 0.0001 0.0016 0.0054 0 0.0006 0 0.0001 0 0 0 0.0001 0.0048 0.0002 0 0 0
Cell Periphery 0.0004 0.0004 0.0006 0 0.0008 0.0003 0.0002 0.0003 0.0001 0.0002 0 0 0.0001 0.0004 0.0003 0.0001 0.0001 0
Cytoplasm 0.0015 0 0.0002 0.0001 0.0065 0.0001 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0
Cytoplasmic Foci 0.009 0.0001 0.0074 0.0163 0.0224 0.0003 0.0015 0.0001 0.0004 0 0 0 0.0011 0.0015 0.0003 0 0 0
Eisosomes 0.0012 0.0019 0.0012 0.0005 0.0014 0.0014 0.001 0.0005 0.0016 0.0005 0.0004 0.0002 0.0008 0.0004 0.0007 0.0108 0.0002 0.0002
Endoplasmic Reticulum 0.0014 0 0 0 0.0013 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endosome 0.0086 0.0002 0.0002 0.0013 0.0025 0.0002 0.0004 0.0001 0.0001 0.0001 0 0 0.0003 0.0011 0.0004 0 0.0007 0
Golgi 0.0021 0.0018 0.0002 0.0013 0.0018 0.0002 0.0011 0.0004 0.0006 0.0002 0.0002 0.0001 0.0004 0.0004 0.0078 0.0001 0.0009 0.0001
Lipid Particles 0.0087 0.0046 0.0033 0.0093 0.0119 0.0015 0.0074 0.0071 0.0082 0.0009 0.0008 0.0035 0.0031 0.0043 0.0016 0.0054 0.0003 0.0013
Mitochondria 0.9112 0.9772 0.9638 0.9218 0.8381 0.9781 0.954 0.9703 0.9831 0.9922 0.9971 0.9902 0.9793 0.9527 0.9797 0.9771 0.992 0.9885
None 0.0026 0 0.0014 0.001 0.0091 0.0001 0.0004 0 0 0 0 0 0.0002 0 0 0 0 0
Nuclear Periphery 0.0057 0 0.0001 0.0001 0.0027 0.0001 0.0005 0 0 0 0 0 0.0001 0.0009 0 0 0 0
Nucleolus 0.0183 0.0019 0.0057 0.0003 0.0219 0.0033 0.0033 0.0046 0.0001 0.0004 0 0 0.003 0.0041 0.001 0.0002 0 0.0001
Nucleus 0.0017 0.0002 0.0004 0.0001 0.0148 0.0006 0.0006 0.0004 0 0.0001 0 0 0.0001 0.0029 0.0001 0 0 0
Peroxisomes 0.0038 0.0012 0.0025 0.0387 0.0174 0.0006 0.0045 0.001 0.0014 0.0009 0.0007 0.0001 0.0009 0.0004 0.0009 0.0012 0.0001 0.0001
Punctate Nuclear 0.0016 0 0.0051 0.0036 0.02 0 0.0005 0 0 0 0 0 0.0002 0 0 0 0 0
Vacuole 0.0014 0.0009 0.0014 0.0002 0.0024 0.0022 0.0009 0.0012 0.0001 0.0004 0 0.0001 0.0002 0.0031 0.0008 0.0002 0.0004 0.0004
Vacuole Periphery 0.0046 0.0068 0.0055 0.0015 0.0019 0.0106 0.0078 0.0129 0.002 0.0034 0.0004 0.0057 0.0042 0.0194 0.0045 0.0037 0.0051 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.7712 20.4983 20.078 22.4549 23.7841 42.1683 46.0112 48.9597 64.4026 55.4441
Translational Efficiency 0.7979 0.8634 0.9048 1.0891 0.6936 1.2778 1.1945 1.4095 1.1804 1.1216

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
162 907 1903 1119 1683 1914 442 1052 1845 2821 2345 2171

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2943.12 2831.19 4338.14 3410.90 4284.65 3144.26 3832.78 3852.67 4166.86 3043.60 4242.89 3624.97
Standard Deviation 864.93 1279.93 1190.35 909.57 1455.06 931.70 977.91 1066.88 1463.26 1066.34 1170.12 1013.28
Intensity Change Log 2 — -0.055938 0.559730 0.212806 — -0.446457 -0.160786 -0.153319 — -0.274503 0.176948 0.007128

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007045 0.013347 0.006235 0.010719 0.003749 0.004608 0.008971 0.005376 0.004038 0.007418 0.006751 0.008130
Bud Neck 0.003157 0.004066 0.001259 0.002917 0.000963 0.000825 0.012172 0.001376 0.001155 0.001867 0.003316 0.002170
Bud Site 0.002170 0.003717 0.000686 0.002682 0.000483 0.001012 0.012377 0.001675 0.000631 0.001882 0.002890 0.002194
Cell Periphery 0.000107 0.000748 0.000051 0.000463 0.000037 0.000113 0.000712 0.000170 0.000043 0.000317 0.000175 0.000321
Cytoplasm 0.000239 0.002340 0.000164 0.000326 0.000179 0.000221 0.000533 0.000224 0.000184 0.000903 0.000234 0.000277
Cytoplasmic Foci 0.040915 0.037649 0.013526 0.011353 0.035876 0.012410 0.056853 0.009707 0.036319 0.020525 0.021693 0.010555
Eisosomes 0.006633 0.012266 0.002827 0.005239 0.006688 0.002607 0.004519 0.004185 0.006683 0.005713 0.003146 0.004728
Endoplasmic Reticulum 0.000435 0.000228 0.000032 0.000213 0.000063 0.000061 0.001165 0.000073 0.000095 0.000115 0.000245 0.000145
Endosome 0.000781 0.000804 0.000298 0.001172 0.000100 0.000342 0.002726 0.000491 0.000160 0.000491 0.000756 0.000842
Golgi 0.015248 0.013781 0.007058 0.013704 0.011072 0.009849 0.016493 0.010704 0.011438 0.011113 0.008837 0.012250
Lipid Particles 0.004406 0.010044 0.003014 0.002915 0.010797 0.003066 0.011809 0.002181 0.010236 0.005309 0.004672 0.002559
Mitochondria 0.875665* 0.835760* 0.940756* 0.898372* 0.894260* 0.941941* 0.775987* 0.936765* 0.892627* 0.907802* 0.909700* 0.916976*
Mitotic Spindle 0.002491 0.000633 0.001111 0.001381 0.000462 0.000243 0.009545 0.000718 0.000640 0.000368 0.002701 0.001060
None 0.005069 0.005670 0.003867 0.005473 0.009391 0.004270 0.005319 0.003929 0.009012 0.004720 0.004141 0.004725
Nuclear Periphery 0.000567 0.000311 0.000404 0.000857 0.000844 0.000198 0.002910 0.000390 0.000820 0.000234 0.000876 0.000631
Nuclear Periphery Foci 0.000464 0.000277 0.000113 0.000274 0.000233 0.000112 0.000874 0.000108 0.000253 0.000165 0.000256 0.000194
Nucleolus 0.002578 0.005742 0.000796 0.001349 0.002364 0.000901 0.003819 0.000908 0.002383 0.002457 0.001366 0.001135
Nucleus 0.000236 0.001554 0.000037 0.000179 0.000138 0.000109 0.000338 0.000142 0.000146 0.000573 0.000093 0.000161
Peroxisomes 0.019241 0.033194 0.009499 0.006344 0.013781 0.006989 0.015024 0.007982 0.014260 0.015414 0.010540 0.007138
Vacuole 0.005170 0.010052 0.002552 0.009638 0.002238 0.004195 0.023080 0.003921 0.002495 0.006078 0.006422 0.006868
Vacuole Periphery 0.007382 0.007818 0.005715 0.024431 0.006283 0.005927 0.034775 0.008975 0.006379 0.006535 0.011192 0.016942

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.46 0.21 -0.90 1.07 -2.80 -1.07 -3.21 -1.96 -0.79 2.11 -3.58 -3.31 -4.17 -0.63 -1.34
Bud Neck -0.83 1.98 0.17 1.41 -3.09 0.73 -6.45 -1.53 -2.28 6.20 -2.62 -5.41 -3.05 -0.95 2.43
Bud Site -1.13 1.26 -0.49 0.83 -2.82 -1.21 -4.51 -1.92 -0.95 3.96 -3.10 -4.13 -3.15 -0.68 0.87
Cell Periphery -1.50 2.39 -3.79 0.64 -4.49 -1.74 -4.37 -4.24 -1.18 3.45 -1.94 -4.16 -5.51 -0.06 -2.52
Cytoplasm -1.88 1.31 -1.04 1.78 -2.12 -0.20 -2.32 -0.39 -0.16 2.20 -1.89 -0.44 -1.05 1.68 -0.94
Cytoplasmic Foci 0.35 3.01 3.22 8.07 1.04 9.86 -3.25 10.66 1.75 7.52 6.53 5.51 10.91 6.54 6.08
Eisosomes -2.73 2.22 0.75 5.05 -3.11 9.30 2.24 3.55 -2.59 0.31 1.72 7.80 3.15 1.58 -2.99
Endoplasmic Reticulum 0.69 1.34 0.73 0.27 -5.11 0.09 -1.09 -0.84 -0.77 1.08 -0.62 -0.78 -1.49 -1.28 0.53
Endosome -0.05 1.42 -0.93 -1.23 -3.23 -3.72 -3.57 -2.97 -1.02 3.00 -4.53 -3.98 -4.42 -2.15 -0.42
Golgi 0.27 1.52 0.28 0.03 -3.96 0.87 -2.03 0.21 -0.50 2.03 0.24 2.06 -0.54 -0.84 -2.68
Lipid Particles -2.66 0.84 0.84 4.68 0.11 7.09 -0.44 7.91 1.76 4.46 4.59 5.15 7.43 4.27 3.25
Mitochondria 2.05 -3.65 -1.20 -6.06 6.40 -8.50 8.22 -6.69 1.04 -11.22 -2.66 -2.84 -4.11 -1.70 -1.32
Mitotic Spindle 1.72 1.29 0.91 -1.36 -0.55 2.28 -3.44 -0.83 -1.51 3.31 1.84 -3.81 -1.34 -2.22 2.62
None -0.79 1.74 -0.54 0.41 -4.31 11.30 7.83 11.76 1.15 3.53 10.25 11.91 10.01 -0.01 -2.31
Nuclear Periphery 1.71 1.08 -1.57 -4.10 -3.38 1.87 -3.07 1.29 -3.42 4.34 1.85 -0.17 0.58 -5.56 1.77
Nuclear Periphery Foci 1.47 2.80 1.45 0.03 -3.54 1.35 -4.41 1.41 0.17 6.53 1.07 -0.04 0.70 -1.07 1.72
Nucleolus -2.30 1.71 1.12 4.61 -1.68 3.58 -2.32 3.32 -0.11 5.35 -0.15 2.69 3.01 3.58 0.93
Nucleus -1.85 1.66 0.38 1.94 -3.38 0.58 -2.97 -0.27 -0.58 1.50 -1.81 1.69 -0.44 1.67 -1.58
Peroxisomes -2.33 1.90 2.48 7.53 1.72 3.98 -0.43 2.87 -0.57 2.45 -0.62 2.17 4.20 5.11 2.34
Vacuole -2.50 2.06 -2.49 0.17 -5.03 -2.77 -7.54 -1.96 0.23 6.73 -4.87 -5.45 -5.01 -0.83 -0.46
Vacuole Periphery -0.15 0.58 -4.71 -6.32 -7.67 0.38 -6.24 -1.98 -2.33 5.54 -0.17 -3.87 -6.97 -7.14 -3.39
Standard name
Human Ortholog
Description T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Gcv1

Gcv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available