Standard name
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 1.0 1.0 0.95 – 0.87 0.82 0.74 0.61 0.65 0.99 0.99 0.99 0.98 0.99 0.97 0.93 0.97 0.91 0.9 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0 0.2 – 0.33 0.42 0.42 0.62 0.58 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 2 2
Bud 2 0 2 0 0 1 – 2 2 1 2 1 0 1 0 0 0 0 1 0 1 4 7 9
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 0 0 – 0 0 0 0 0 2 1 2 7 1 11 0 0 0 0 0 0
Cytoplasm 490 305 74 21 73 122 – 119 117 84 58 80 574 539 667 290 224 227 451 298 75 268 429 354
Endoplasmic Reticulum 1 0 0 0 0 1 – 0 0 1 0 3 3 1 0 13 8 10 0 0 0 1 2 1
Endosome 0 0 0 0 0 0 – 1 1 0 1 2 2 1 0 2 1 1 4 0 0 4 5 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 1 2 0 0 3 1 2
Mitochondria 11 4 4 0 2 26 – 45 59 48 59 71 0 0 1 3 3 5 9 2 1 3 1 1
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 – 0 0 1 1 1 0 1 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 0 0 2 0 0 3 3 2
Vac/Vac Membrane 2 1 0 0 0 0 – 2 1 3 2 2 1 1 2 1 2 7 2 1 0 3 4 0
Unique Cell Count 501 308 76 21 73 129 137 142 113 95 123 580 542 671 297 227 233 487 307 83 300 465 388
Labelled Cell Count 507 311 81 22 75 151 169 180 138 123 164 583 546 674 319 240 264 487 307 83 300 465 388


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.9 3.6 3.7 4.8 3.7 – 3.4 3.2 3.7 3.6 3.3 5.3 5.4 5.3 6.6 6.1 6.3 5.3 5.4 5.7
Std Deviation (1e-4) 0.6 0.6 1.4 0.5 2.7 1.7 – 1.0 1.4 1.8 2.0 1.0 0.9 1.0 0.9 1.8 1.2 1.9 1.7 1.4 1.5
Intensity Change (Log2) – – – 0.01 0.42 0.02 – -0.11 -0.17 0.01 -0.01 -0.12 0.54 0.58 0.54 0.87 0.75 0.78 0.54 0.58 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 1.4 -0.9 – -2.5 -3.2 -4.2 -5.6 -5.3 1.2 1.9 1.9 0.1 0.8 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9208 0.7102 0.6088 -0.1123 1.0217 0.5636 0.6202 0.0607 0.3862 0.0089 0.0125 0.1352 2.2871 1.9645 2.0257 1.8925 1.7313 1.787
Actin 0.012 0.0014 0.0183 0.0002 0.0012 0.03 0.0446 0.0069 0.0199 0.0164 0.0083 0.0015 0.0284 0.0028 0.0094 0.0022 0.0161 0.0032
Bud 0.0008 0.0001 0.0003 0.0001 0.0021 0.0015 0.014 0.0121 0.0019 0.0012 0.0002 0.0002 0.0007 0.0092 0.0052 0.0004 0.0017 0.0008
Bud Neck 0.003 0.0001 0.0003 0.0001 0.0003 0.0017 0.0071 0.0002 0.0017 0.0005 0.0002 0.0006 0.0028 0.0004 0.0008 0.0006 0.0009 0.0012
Bud Periphery 0.001 0 0.0002 0 0.0036 0.0048 0.0065 0.0072 0.0032 0.003 0.0002 0.0003 0.0009 0.0057 0.0047 0.0003 0.0018 0.0009
Bud Site 0.008 0.0043 0.001 0 0.0009 0.0002 0.0208 0.0068 0.0062 0.001 0.0003 0.0002 0.0102 0.0216 0.0034 0.0034 0.0018 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0 0.0002 0.0001 0.0011 0.0002 0.0003 0.0001 0.0001 0 0.0003 0.0004 0.0002 0.0001 0.0003 0
Cytoplasm 0.265 0.4069 0.3763 0.4563 0.3303 0.4417 0.2112 0.3751 0.3089 0.3374 0.295 0.4575 0.2464 0.3774 0.3941 0.3987 0.4451 0.4836
Cytoplasmic Foci 0.0774 0.0174 0.0277 0.005 0.0272 0.0234 0.0594 0.0053 0.0267 0.023 0.0252 0.012 0.0344 0.0063 0.0095 0.015 0.0106 0.0085
Eisosomes 0.0002 0 0.0002 0.0002 0.0001 0.0001 0.0004 0 0.0002 0.0001 0.0005 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.0095 0.0009 0.0019 0.0017 0.0063 0.0014 0.0064 0.0018 0.005 0.0008 0.0039 0.0018 0.0033 0.0011 0.0026 0.0014 0.0021 0.0015
Endosome 0.0554 0.0011 0.0234 0.0031 0.1752 0.001 0.0517 0.0014 0.0142 0.0052 0.0318 0.0103 0.0228 0.0016 0.01 0.0098 0.0094 0.0021
Golgi 0.0274 0.0007 0.0103 0.0001 0.0114 0.0001 0.0314 0.0004 0.0047 0.0064 0.0133 0.0031 0.0124 0.0005 0.004 0.0134 0.0074 0.0001
Lipid Particles 0.0329 0.0007 0.0122 0.0002 0.0034 0.0001 0.0257 0.0002 0.0035 0.0072 0.0102 0.0014 0.0151 0.0002 0.0033 0.0014 0.0047 0.0001
Mitochondria 0.0145 0.0001 0.0162 0.0002 0.0129 0.0003 0.0258 0.0006 0.0036 0.0011 0.0035 0.0092 0.0212 0.001 0.0019 0.002 0.0011 0.0003
None 0.4217 0.5627 0.5033 0.5291 0.2632 0.4902 0.413 0.5693 0.5748 0.5843 0.5637 0.4869 0.5447 0.5593 0.5398 0.5349 0.4344 0.4747
Nuclear Periphery 0.026 0.0003 0.0005 0.0008 0.0336 0.0002 0.0209 0.0006 0.0018 0.0005 0.0066 0.0047 0.0082 0.0008 0.0026 0.0013 0.0043 0.0011
Nucleolus 0.0008 0.0001 0.0001 0.0001 0.0011 0.0001 0.0015 0.0001 0.0003 0.0001 0.0002 0.0001 0.0011 0.0007 0.0003 0.0004 0.0018 0.0013
Nucleus 0.0047 0.0006 0.0008 0.001 0.0672 0.0005 0.0193 0.0013 0.0019 0.0011 0.003 0.0045 0.0205 0.007 0.0026 0.0024 0.0324 0.0156
Peroxisomes 0.0238 0.0013 0.0044 0.0001 0.0011 0.0004 0.0202 0.0086 0.0152 0.0088 0.028 0.0016 0.0191 0.0009 0.0018 0.0036 0.0037 0.0002
Punctate Nuclear 0.0064 0.0006 0.0008 0.0005 0.0061 0.0016 0.011 0.0006 0.0036 0.0012 0.0023 0.0024 0.0042 0.0016 0.0017 0.0074 0.0142 0.0035
Vacuole 0.005 0.0005 0.0013 0.001 0.025 0.0004 0.0053 0.001 0.0018 0.0006 0.002 0.001 0.0021 0.0014 0.0015 0.001 0.0056 0.0009
Vacuole Periphery 0.004 0 0.0003 0.0002 0.0277 0 0.0027 0.0001 0.0005 0.0001 0.0014 0.0005 0.0007 0.0002 0.0004 0.0002 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4871 6.0887 10.5423 16.8278 16.1901 2.2691 12.8257 14.5429 9.0372 13.3982
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1484 1411 319 703 1975 1993 309 1075 3459 3404 628 1778

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.28 690.25 909.93 796.03 658.48 718.88 834.04 776.07 655.82 707.01 872.59 783.96
Standard Deviation 71.05 129.52 93.38 95.29 78.72 94.44 77.28 88.40 75.58 111.24 93.85 91.71
Intensity Change Log 2 — 0.081628 0.480264 0.287331 — 0.126611 0.340977 0.237047 — 0.104401 0.411972 0.262289

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000188 0.000509 0.000202 0.000098 0.000190 0.000079 0.000057 0.000073 0.000189 0.000297 0.000114
Bud Neck 0.010764 0.009314 0.006237 0.003515 0.008919 0.010826 0.003358 0.002479 0.009711 0.010199 0.004820 0.002889
Bud Site 0.002462 0.003068 0.006278 0.016975 0.001909 0.005619 0.001574 0.002433 0.002146 0.004562 0.003963 0.008183
Cell Periphery 0.000095 0.000199 0.000186 0.000224 0.000073 0.000251 0.000118 0.000100 0.000082 0.000229 0.000153 0.000149
Cytoplasm 0.584424* 0.591872* 0.423747* 0.204323* 0.578096* 0.648555* 0.422594* 0.512726* 0.580811* 0.625059* 0.423180* 0.390787*
Cytoplasmic Foci 0.115135 0.152774 0.039402 0.017142 0.124552 0.121084 0.028482 0.030071 0.120512 0.134220 0.034029 0.024959
Eisosomes 0.000160 0.000266 0.000096 0.000066 0.000083 0.000086 0.000051 0.000034 0.000116 0.000161 0.000074 0.000047
Endoplasmic Reticulum 0.000580 0.000880 0.003421 0.003506 0.000245 0.000229 0.001279 0.000694 0.000389 0.000499 0.002367 0.001806
Endosome 0.005343 0.010139 0.006022 0.002505 0.003996 0.007138 0.002575 0.002750 0.004574 0.008382 0.004326 0.002653
Golgi 0.000424 0.001375 0.000093 0.000070 0.000410 0.000857 0.000051 0.000083 0.000416 0.001072 0.000072 0.000078
Lipid Particles 0.005868 0.006314 0.003037 0.002441 0.003723 0.004046 0.001694 0.001078 0.004644 0.004986 0.002376 0.001617
Mitochondria 0.001052 0.002251 0.000270 0.000546 0.001901 0.003517 0.000159 0.000397 0.001537 0.002992 0.000215 0.000456
Mitotic Spindle 0.000486 0.002680 0.000643 0.001660 0.000638 0.005603 0.000526 0.002223 0.000573 0.004391 0.000586 0.002001
None 0.003376 0.015761 0.004489 0.003531 0.002153 0.001985 0.003161 0.004126 0.002678 0.007695 0.003836 0.003891
Nuclear Periphery 0.000367 0.000483 0.001186 0.001867 0.000424 0.000280 0.001030 0.000857 0.000400 0.000364 0.001109 0.001257
Nuclear Periphery Foci 0.000288 0.000656 0.003000 0.000778 0.000173 0.000212 0.000755 0.000507 0.000222 0.000396 0.001895 0.000614
Nucleolus 0.000873 0.001706 0.000568 0.000811 0.000743 0.001017 0.000520 0.000464 0.000799 0.001303 0.000544 0.000601
Nucleus 0.217360* 0.140726* 0.358734* 0.687451* 0.219471* 0.130720* 0.476366* 0.396797* 0.218565* 0.134868* 0.416613* 0.511718*
Peroxisomes 0.001455 0.004018 0.000595 0.000343 0.002361 0.003606 0.000253 0.000172 0.001972 0.003777 0.000427 0.000240
Vacuole 0.049223 0.054820 0.141302 0.051754 0.049793 0.053718 0.055266 0.041595 0.049548 0.054175 0.098969 0.045611
Vacuole Periphery 0.000223 0.000509 0.000186 0.000292 0.000238 0.000462 0.000108 0.000356 0.000231 0.000481 0.000147 0.000331

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.79 -2.82 -7.89 -0.56 1.57 -0.89 -0.37 0.56 1.16 1.47 -1.70 -2.74 -2.81 0.71 1.98
Bud Neck 1.02 3.00 6.09 4.94 0.53 -1.27 2.50 5.63 6.14 0.30 -0.46 3.79 8.28 7.87 0.63
Bud Site -0.98 -1.56 -3.67 -3.47 -2.25 -4.08 -0.02 -1.22 2.96 -0.90 -4.05 -1.53 -3.83 -1.75 -1.99
Cell Periphery -5.19 -6.99 -4.82 -0.58 -0.78 -1.60 -5.42 -3.48 1.33 2.53 -2.23 -9.00 -5.78 1.23 1.30
Cytoplasm -0.59 14.32 38.30 37.17 11.19 -8.85 11.19 7.88 15.19 -5.68 -7.15 17.96 25.03 30.27 0.75
Cytoplasmic Foci -6.46 17.24 25.36 28.12 5.45 0.73 22.07 25.76 25.34 0.14 -3.80 27.84 35.59 37.38 3.42
Eisosomes -1.62 2.96 5.68 2.90 4.33 -0.43 2.93 10.67 11.18 4.31 -1.61 3.44 9.53 3.99 7.00
Endoplasmic Reticulum -1.20 -10.43 -10.36 -8.36 -0.20 0.71 -8.37 -8.56 -8.57 3.93 -1.00 -12.63 -11.92 -9.91 2.68
Endosome -5.07 -1.26 5.17 8.66 6.70 -5.53 1.71 2.72 6.98 0.72 -7.38 -0.35 5.37 10.98 5.03
Golgi -3.69 6.27 6.86 5.21 3.21 -2.68 4.38 3.92 5.13 -0.82 -4.52 6.65 6.46 7.29 0.25
Lipid Particles -0.53 3.73 4.89 4.15 1.41 -0.51 3.20 5.86 5.61 3.27 -0.68 4.62 7.74 6.87 3.90
Mitochondria -2.11 2.53 1.61 3.58 -3.83 -1.76 3.44 2.86 4.01 -1.59 -2.49 4.16 3.27 5.11 -2.60
Mitotic Spindle -2.37 -0.01 -1.26 1.43 -1.23 -4.02 0.21 -1.71 1.87 -1.75 -4.64 0.15 -2.01 2.21 -2.03
None -5.05 0.39 0.12 5.09 -0.31 0.41 -1.51 -4.27 -4.36 -1.73 -4.81 -0.96 -3.26 3.79 -1.64
Nuclear Periphery -2.24 -15.38 -18.78 -16.28 -6.52 5.26 -11.22 -12.56 -16.38 1.96 1.46 -18.69 -21.76 -21.51 -2.79
Nuclear Periphery Foci -4.34 -10.43 -7.42 -2.78 7.77 -1.32 -5.19 -5.21 -4.24 2.27 -4.26 -11.23 -8.64 -4.81 7.95
Nucleolus -3.42 4.03 0.83 3.85 -3.91 -1.20 1.43 2.47 2.36 0.52 -2.89 3.27 2.64 4.16 -1.34
Nucleus 12.22 -9.70 -39.82 -46.88 -18.43 16.66 -16.25 -19.46 -30.68 4.11 20.66 -18.00 -36.84 -48.90 -7.58
Peroxisomes -4.16 2.63 3.90 6.87 1.26 -1.73 4.53 4.89 6.02 1.80 -3.67 5.10 6.14 8.83 2.02
Vacuole -2.18 -12.77 -5.15 -3.80 9.81 -1.14 -3.17 0.26 1.15 3.14 -2.28 -11.87 -3.44 -1.83 10.03
Vacuole Periphery -4.82 1.56 -1.55 2.86 -2.30 -3.58 4.25 -0.67 0.76 -1.42 -5.67 4.19 -0.99 1.56 -1.79
Standard name
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
Localization
Cell Percentages cytoplasm (32%), vacuole (1%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fyv1

Fyv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fyv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available