Standard name
Human Ortholog
Description Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.55 0.73 0.19 0.12 0.15 0.1 – 0.07 0.06 0.05 0.05 0.21 0.32 0.32 0.38 0.41 0.33 0.26 0.32 0.36 0.18 0.15 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0
Endosome 0.17 0 0 0 0 0 0 – 0 0 0 0 0 0 0.12 0.1 0.07 0.07 0.07 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.25 0 0.08 0 0.47 0.12 – 0.35 0.31 0.38 0.36 0.05 0 0 0 0 0 0.11 0.05 0.1 0.05 0.06 0
Nucleus 0.13 0.14 0.12 0.71 0.79 0.61 0.79 – 0.73 0.74 0.75 0.76 0.62 0.5 0.4 0.17 0.2 0.29 0.15 0.24 0.16 0.23 0.28 0.33
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0.06 0.05 0.09 0.08 0 0 0 0 0 0 0 0 0 0.07 0 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.06 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.08 0.18 0.12 0.15
Vac/Vac Membrane 0.3 0.21 0.08 0 0 0 0 – 0 0 0 0 0.15 0.15 0.24 0.4 0.34 0.38 0.17 0.24 0.16 0.17 0.17 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 3 0 1 0 0 1
Bud 0 0 0 1 0 6 4 – 4 9 11 7 0 0 0 1 1 0 2 1 1 3 11 11
Bud Neck 0 0 8 1 3 2 3 – 1 5 2 5 0 0 0 1 0 0 2 1 1 1 6 7
Bud Site 0 0 0 1 1 7 6 – 10 8 30 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 101 39 106 27 31 64 48 – 34 32 24 26 27 63 102 67 50 33 82 37 81 28 43 50
Endoplasmic Reticulum 1 0 0 0 1 0 0 – 0 1 0 0 1 1 1 16 3 5 3 0 1 2 2 2
Endosome 43 0 3 4 6 0 7 – 1 3 3 3 4 6 38 18 8 7 24 2 5 4 15 9
Golgi 1 0 1 0 0 0 0 – 0 0 0 0 0 1 0 2 0 0 4 0 2 0 1 1
Mitochondria 19 18 2 11 5 194 56 – 172 157 198 193 6 3 5 4 4 4 35 6 23 7 18 9
Nucleus 32 10 17 98 210 255 366 – 361 372 393 406 81 97 128 30 25 29 48 29 36 36 80 112
Nuclear Periphery 0 0 0 0 0 0 0 – 0 2 4 6 0 0 1 1 0 0 3 0 1 0 2 1
Nucleolus 0 0 0 1 0 4 5 – 32 26 48 42 0 0 1 0 0 0 9 0 3 11 11 22
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 4 1 3 10 15 12 28 – 15 17 8 9 0 3 3 4 3 0 31 8 18 29 34 51
Vac/Vac Membrane 74 15 11 2 11 6 6 – 4 6 0 4 19 30 77 71 42 38 53 28 37 26 51 49
Unique Cell Count 246 71 146 139 266 417 462 496 503 522 537 130 195 320 176 123 99 324 119 230 160 293 343
Labelled Cell Count 275 83 151 156 283 550 529 634 639 721 720 138 204 358 215 136 116 324 119 230 160 293 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.0 4.9 5.0 5.6 4.1 5.0 – 4.7 4.9 4.7 4.7 5.3 5.2 5.5 6.0 6.0 5.7 4.7 4.8 4.9
Std Deviation (1e-4) 1.0 0.5 1.1 1.8 1.2 1.4 1.6 – 1.8 1.5 1.3 1.0 1.5 1.1 1.7 1.7 1.5 1.2 1.9 1.7 1.6
Intensity Change (Log2) – – – 0.02 0.2 -0.28 0.03 – -0.06 0.01 -0.05 -0.07 0.12 0.09 0.16 0.28 0.3 0.21 -0.05 -0.02 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.7 5.5 5.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8421 -1.307 -1.2522 -1.4882 -1.5311 -1.3917 0.0437 0.2546 0.218 -0.2512 -0.0012 -0.0457 2.3004 2.3615 2.2769 3.215 2.1111 2.4549
Actin 0.0514 0.0006 0.008 0.0094 0.0035 0.0088 0.0409 0.0005 0.0071 0.0007 0.0113 0.0007 0.0074 0.0001 0.0004 0.0004 0.0013 0.0051
Bud 0.0009 0.0001 0.0003 0.0006 0.0001 0.0002 0.0007 0.0033 0.0084 0.0001 0.0003 0.0002 0.0001 0.0001 0.0006 0.0001 0.0003 0.0002
Bud Neck 0.0061 0.0005 0.0018 0.0021 0.001 0.0036 0.0032 0.0008 0.001 0.0006 0.0031 0.0054 0.0009 0.0004 0.0006 0.0008 0.002 0.0072
Bud Periphery 0.0013 0.0001 0.0004 0.0015 0.0003 0.0003 0.0016 0.0006 0.0025 0.0001 0.0006 0.0003 0.0001 0 0.0008 0.0001 0.0005 0.0003
Bud Site 0.0098 0.0007 0.0083 0.0035 0.0004 0.0003 0.0044 0.0141 0.0064 0.0003 0.0004 0.0004 0.0012 0.0006 0.0018 0.0002 0.0007 0.0004
Cell Periphery 0.0004 0 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.097 0.1424 0.0781 0.0935 0.0364 0.1051 0.0905 0.1222 0.076 0.0762 0.1513 0.0818 0.0481 0.062 0.0593 0.0593 0.1035 0.0586
Cytoplasmic Foci 0.0362 0.0053 0.0157 0.0142 0.0153 0.0152 0.0253 0.0084 0.0102 0.0213 0.0095 0.0069 0.0172 0.0048 0.0063 0.0013 0.0101 0.0071
Eisosomes 0.0007 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0073 0.0038 0.0041 0.0043 0.0028 0.0024 0.005 0.0039 0.002 0.0016 0.0047 0.0015 0.0048 0.0015 0.0014 0.0013 0.0021 0.0012
Endosome 0.0423 0.0044 0.0142 0.0281 0.0532 0.0078 0.0253 0.0046 0.0075 0.0044 0.0116 0.0075 0.0112 0.0009 0.0027 0.0013 0.0166 0.0069
Golgi 0.0192 0.0002 0.004 0.0161 0.0161 0.0023 0.0109 0.0003 0.0031 0.0035 0.0112 0.0004 0.0021 0 0.0003 0 0.0047 0.0016
Lipid Particles 0.026 0.0009 0.0058 0.011 0.0525 0.0041 0.0322 0.0011 0.0087 0.0136 0.0228 0.0027 0.0072 0.0001 0.0006 0.0001 0.0074 0.0036
Mitochondria 0.0275 0.0004 0.0025 0.0141 0.0143 0.0129 0.0057 0.0008 0.0099 0.0097 0.0179 0.0009 0.001 0.0003 0.001 0.0007 0.003 0.0041
None 0.133 0.2103 0.1372 0.1167 0.2011 0.1708 0.1679 0.2137 0.1982 0.1364 0.1762 0.148 0.1337 0.2445 0.1685 0.2716 0.2134 0.1477
Nuclear Periphery 0.0264 0.0277 0.0326 0.0214 0.0333 0.0273 0.0265 0.0229 0.0177 0.0128 0.0267 0.0128 0.0229 0.005 0.0165 0.0091 0.0173 0.0061
Nucleolus 0.007 0.0061 0.0079 0.0178 0.0067 0.0084 0.0061 0.0094 0.0087 0.0098 0.0107 0.014 0.007 0.0055 0.0076 0.0053 0.0094 0.0137
Nucleus 0.3919 0.5091 0.5937 0.5656 0.3821 0.5477 0.4886 0.5101 0.5527 0.6564 0.3865 0.6184 0.6724 0.63 0.677 0.5989 0.5633 0.6767
Peroxisomes 0.0262 0.0004 0.0019 0.008 0.0048 0.003 0.0272 0.001 0.0055 0.0091 0.0105 0.0007 0.0118 0.0001 0.0013 0.0001 0.0017 0.0026
Punctate Nuclear 0.0833 0.0844 0.079 0.0635 0.1707 0.0768 0.0315 0.0793 0.0711 0.0417 0.1404 0.0952 0.0485 0.043 0.0499 0.0483 0.0387 0.0549
Vacuole 0.0044 0.0022 0.0033 0.006 0.0032 0.0021 0.0043 0.0022 0.0022 0.0007 0.0028 0.0017 0.0012 0.0008 0.0027 0.0008 0.0026 0.0012
Vacuole Periphery 0.0017 0.0004 0.0009 0.0021 0.0023 0.0007 0.0016 0.0005 0.0009 0.0008 0.001 0.0004 0.0007 0.0001 0.0005 0.0002 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 2.8344 2.1033 10.5398 8.2219 7.9224 7.5229 9.1878 8.0774 10.0299
Translational Efficiency 1.4364 4.6999 4.9505 0.9202 1.532 2.4956 2.1296 2.2156 1.6155 1.5502

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2319 1510 97 1417 1230 2243 1534 1571 3549 3753 1631 2988

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 653.44 726.72 982.09 993.83 645.73 758.01 836.87 1027.81 650.77 745.42 845.51 1011.70
Standard Deviation 71.52 83.47 99.95 167.44 76.15 94.25 114.22 167.25 73.25 91.36 118.50 168.20
Intensity Change Log 2 — 0.153345 0.587800 0.604944 — 0.231286 0.374072 0.670571 — 0.192610 0.485524 0.637936

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000052 0.000316 0.001014 0.001034 0.000093 0.000193 0.000361 0.000729 0.000066 0.000242 0.000400 0.000874
Bud Neck 0.007516 0.008544 0.002627 0.021797 0.002362 0.010620 0.004455 0.028640 0.005730 0.009785 0.004346 0.025395
Bud Site 0.001759 0.003878 0.001689 0.023424 0.000804 0.006164 0.002146 0.017384 0.001428 0.005244 0.002119 0.020248
Cell Periphery 0.000147 0.000168 0.000231 0.000357 0.000171 0.000171 0.000114 0.000174 0.000156 0.000170 0.000121 0.000261
Cytoplasm 0.264514* 0.254995* 0.046872 0.134690* 0.225939* 0.219896* 0.054747 0.129434* 0.251145* 0.234018* 0.054279 0.131926*
Cytoplasmic Foci 0.066479 0.077110 0.007273 0.005043 0.044203 0.039343 0.001136 0.002156 0.058759 0.054539 0.001501 0.003525
Eisosomes 0.000052 0.000062 0.000120 0.000033 0.000049 0.000047 0.000021 0.000026 0.000051 0.000053 0.000027 0.000029
Endoplasmic Reticulum 0.000955 0.002846 0.011201 0.008315 0.001054 0.001313 0.003428 0.005841 0.000989 0.001930 0.003890 0.007014
Endosome 0.001350 0.003808 0.003008 0.007759 0.001672 0.002898 0.000709 0.001729 0.001462 0.003264 0.000845 0.004589
Golgi 0.001078 0.001655 0.000139 0.008222 0.000569 0.001729 0.000067 0.000871 0.000901 0.001699 0.000071 0.004357
Lipid Particles 0.008734 0.009347 0.004012 0.000946 0.006142 0.005455 0.000433 0.000467 0.007835 0.007021 0.000646 0.000694
Mitochondria 0.005825 0.011107 0.000657 0.006305 0.005264 0.009792 0.000667 0.004177 0.005630 0.010321 0.000667 0.005186
Mitotic Spindle 0.004281 0.007116 0.000185 0.056048 0.002359 0.010184 0.004270 0.045234 0.003615 0.008950 0.004027 0.050362
None 0.011399 0.014810 0.003037 0.010439 0.017651 0.012878 0.012472 0.010310 0.013566 0.013656 0.011911 0.010371
Nuclear Periphery 0.002372 0.002851 0.001802 0.003125 0.001569 0.002046 0.001138 0.003457 0.002094 0.002370 0.001178 0.003300
Nuclear Periphery Foci 0.000745 0.001903 0.002021 0.001645 0.000780 0.001068 0.000475 0.000823 0.000757 0.001404 0.000567 0.001213
Nucleolus 0.006488 0.010339 0.003592 0.001332 0.006184 0.008175 0.002988 0.001496 0.006383 0.009046 0.003024 0.001418
Nucleus 0.608074* 0.572669* 0.860470* 0.666887* 0.676644* 0.656221* 0.906105* 0.722380* 0.631839* 0.622604* 0.903391* 0.696064*
Peroxisomes 0.003926 0.006498 0.000612 0.001081 0.001990 0.005040 0.000132 0.000286 0.003255 0.005627 0.000161 0.000663
Vacuole 0.003962 0.008773 0.049191 0.038111 0.004075 0.006030 0.004049 0.021624 0.004001 0.007133 0.006734 0.029443
Vacuole Periphery 0.000291 0.001203 0.000245 0.003406 0.000427 0.000737 0.000088 0.002762 0.000338 0.000924 0.000097 0.003067

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.06 -7.15 -16.86 -10.84 1.65 -4.41 -12.10 -15.25 -13.39 -8.37 -10.57 -15.31 -22.89 -17.52 -9.98
Bud Neck -1.36 4.59 -9.25 -8.33 -11.91 -7.92 -5.35 -14.47 -9.06 -12.16 -5.47 -0.51 -16.08 -11.93 -15.32
Bud Site -3.01 -2.26 -8.08 -6.14 -7.12 -5.91 -3.01 -9.71 -4.38 -7.92 -6.07 -1.98 -12.23 -7.12 -10.33
Cell Periphery -1.63 -3.34 -2.23 -1.44 1.00 0.06 1.88 0.06 -0.02 -5.85 -0.93 2.13 -1.89 -1.16 -4.51
Cytoplasm 1.21 21.47 27.38 22.39 -4.33 0.68 24.21 18.74 22.14 -7.91 2.98 40.70 34.02 31.21 -10.00
Cytoplasmic Foci -2.13 20.84 25.29 21.33 3.67 1.77 17.16 17.21 20.37 0.08 1.85 30.18 30.41 29.04 0.36
Eisosomes -3.68 -6.22 2.70 5.87 6.70 1.17 8.56 3.25 2.18 -7.12 -1.02 8.30 4.48 5.20 -5.06
Endoplasmic Reticulum -11.64 -8.76 -20.20 -15.02 3.37 -2.07 -14.71 -19.67 -18.96 -9.05 -9.84 -17.23 -28.30 -24.43 -10.12
Endosome -6.27 -3.91 -2.33 2.14 1.79 -3.32 4.15 2.05 5.26 -2.83 -7.06 3.37 -1.36 4.36 -3.43
Golgi -1.43 3.11 -0.90 -0.27 -2.06 -2.97 3.40 1.05 3.53 -1.67 -2.49 4.15 -0.36 1.28 -2.46
Lipid Particles -0.43 5.83 12.37 12.13 5.39 1.10 11.08 10.58 11.10 -4.09 1.55 15.82 15.61 16.36 -1.80
Mitochondria -3.83 8.94 2.89 5.57 -7.02 -2.92 3.45 1.09 5.76 -8.31 -4.87 8.45 2.94 7.96 -10.79
Mitotic Spindle -1.98 3.62 -9.58 -8.59 -10.79 -4.46 -2.05 -10.25 -7.65 -9.15 -4.35 -1.18 -13.83 -11.35 -12.57
None -2.60 10.09 2.78 4.85 -8.90 2.80 4.05 6.17 3.79 1.69 0.00 2.91 6.40 5.85 1.19
Nuclear Periphery -1.00 -0.33 -10.83 -5.53 -9.46 -2.51 -0.28 -13.90 -11.94 -14.96 -1.31 2.60 -16.15 -12.34 -20.72
Nuclear Periphery Foci -4.15 -3.54 -2.96 1.91 2.19 -1.19 0.73 -2.11 0.13 -3.28 -2.97 0.26 -3.36 0.96 -4.02
Nucleolus -3.50 5.52 9.13 9.86 1.94 -3.39 5.79 6.82 9.63 1.58 -4.53 8.12 10.88 13.87 2.94
Nucleus 3.50 -8.36 -12.37 -14.17 3.35 2.03 -24.78 -7.31 -10.51 19.37 1.44 -40.14 -15.53 -16.72 24.13
Peroxisomes -3.37 8.70 9.11 10.46 1.77 -4.26 5.47 5.30 8.89 -2.04 -4.39 11.21 10.72 13.27 -1.66
Vacuole -6.19 -5.46 -17.34 -15.65 1.66 -3.02 -3.39 -15.72 -14.86 -13.30 -6.56 -6.11 -23.37 -21.59 -15.88
Vacuole Periphery -5.16 0.02 -2.00 0.85 -2.01 -2.16 3.28 -1.03 -0.37 -1.80 -5.82 5.70 -2.02 0.06 -2.87
Standard name
Human Ortholog
Description Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose
Localization
Cell Percentages nucleus (73%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nrg1

Nrg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available