Standard name
Human Ortholog
Description Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 1.0 0.96 0.97 0.89 0.85 0.79 0.69 0.66 0.63 0.65 0.98 0.99 0.99 0.93 0.92 0.96 0.93 0.71 0.91 0.94 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.21 0.25 0.37 0.4 0.48 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.06 0.05 0.08 0.06 0 0.16 0 0 0 0.08 0.05 0 0 0.2 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 1 0 0 0 0 3 2
Bud 0 1 0 0 0 2 1 4 8 9 6 3 0 1 2 0 0 0 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 0 0 6 1 2 3 1 4 2 3 0 1 6 10 7 6 0 0 0 0 0 1
Cytoplasm 215 85 48 87 223 149 244 199 169 188 149 149 331 399 485 403 196 308 201 79 74 229 443 371
Endoplasmic Reticulum 1 0 0 0 0 1 0 1 0 0 2 0 1 0 1 8 9 9 1 0 2 0 3 1
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 2 0 1 4 2 0 2 1 0 2 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 1 1 0 0 0 1 5 2
Mitochondria 1 2 0 2 5 19 60 62 91 113 113 70 1 0 1 4 2 2 1 0 1 1 3 1
Nucleus 0 2 0 0 3 2 2 5 8 4 3 5 4 5 0 6 4 0 0 3 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 4 1
Vac/Vac Membrane 0 1 0 4 6 11 17 12 20 18 6 36 9 8 11 33 10 10 3 22 0 3 1 3
Unique Cell Count 217 88 48 91 231 167 288 253 244 285 235 228 339 403 490 433 214 321 217 111 81 243 482 391
Labelled Cell Count 217 94 48 93 243 186 327 286 297 338 282 268 350 415 507 473 232 337 217 111 81 243 482 391


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 8.4 8.7 7.0 6.8 5.6 5.3 5.3 4.5 4.6 4.2 5.1 7.9 8.0 8.0 11.7 11.7 11.1 7.9 8.0 8.4
Std Deviation (1e-4) 0.9 1.4 3.1 1.5 1.5 1.2 1.6 1.5 1.2 1.2 1.0 1.2 1.2 1.1 1.2 2.1 1.6 1.9 1.7 1.6 1.8
Intensity Change (Log2) – – – -0.31 -0.34 -0.64 -0.71 -0.72 -0.95 -0.92 -1.04 -0.77 -0.13 -0.11 -0.12 0.43 0.43 0.35 -0.13 -0.11 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.3 -2.4 -2.9 -3.5 -4.5 -4.8 -5.0 -4.8 -1.1 -0.7 -0.7 -1.9 -2.1 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.5 3.8 0 0 0 4.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.836 3.2108 2.9464 2.3986 2.4613 2.6032 2.7844 3.6741 3.0529 2.594 2.8409 3.2272 2.8957 3.6613 2.9672 2.9373 2.76 3.23
Actin 0.0338 0.0006 0.013 0.0176 0.0634 0.0076 0.0436 0.0027 0.0109 0.0483 0.0164 0.0018 0.0148 0.0007 0.0162 0.0017 0.0101 0.0047
Bud 0.0022 0.002 0.0129 0.0003 0.0008 0.0015 0.0007 0.0002 0.0003 0.001 0.0005 0.0033 0.0004 0.0073 0.0004 0.0001 0.0006 0.0002
Bud Neck 0.0025 0.0002 0.0011 0.0006 0.001 0.0039 0.0073 0.0007 0.0006 0.0032 0.002 0.0031 0.0024 0.0001 0.0004 0.0004 0.0008 0.0009
Bud Periphery 0.002 0.0008 0.0034 0.0002 0.001 0.0006 0.001 0.0001 0.0003 0.0009 0.0005 0.001 0.0003 0.0014 0.0004 0 0.0006 0.0004
Bud Site 0.0042 0.0048 0.0099 0.0016 0.0016 0.0006 0.0075 0.0014 0.0011 0.0028 0.0004 0.0004 0.0015 0.003 0.0024 0.0001 0.0006 0.0021
Cell Periphery 0.0002 0.0001 0.0004 0 0.0002 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.6532 0.9066 0.7992 0.7878 0.701 0.8406 0.6511 0.9109 0.8351 0.686 0.8787 0.8957 0.8395 0.9366 0.8749 0.9229 0.8396 0.9286
Cytoplasmic Foci 0.0385 0.0069 0.0276 0.0145 0.0385 0.0111 0.0545 0.0062 0.0223 0.0347 0.0197 0.0143 0.02 0.0029 0.0153 0.0092 0.0348 0.0061
Eisosomes 0.0003 0 0.0002 0.0001 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001 0 0 0.0002 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0072 0.0021 0.0045 0.0049 0.0054 0.0032 0.0081 0.0013 0.0033 0.0037 0.0027 0.0017 0.0059 0.0014 0.0047 0.0017 0.0054 0.0017
Endosome 0.0491 0.0027 0.0247 0.0095 0.038 0.0123 0.0261 0.0018 0.0156 0.0195 0.0158 0.0136 0.0216 0.0013 0.0134 0.0022 0.0372 0.0097
Golgi 0.0102 0.0001 0.0044 0.0035 0.0195 0.0061 0.0102 0.0006 0.0029 0.0194 0.0023 0.0019 0.007 0.0001 0.0032 0.0003 0.0052 0.0047
Lipid Particles 0.0103 0.0001 0.0016 0.0026 0.0071 0.0119 0.0247 0.0004 0.0023 0.0074 0.0016 0.0052 0.008 0 0.0036 0.0006 0.0028 0.0026
Mitochondria 0.0116 0.0002 0.0058 0.0061 0.018 0.0065 0.011 0.0061 0.0084 0.0227 0.0006 0.0022 0.0124 0.0016 0.0069 0.0002 0.0017 0.0035
None 0.0981 0.0524 0.0583 0.104 0.0352 0.0709 0.0696 0.0583 0.0603 0.0198 0.0364 0.0243 0.0363 0.0363 0.0315 0.045 0.012 0.0107
Nuclear Periphery 0.0213 0.0018 0.0042 0.0047 0.0281 0.0032 0.011 0.0009 0.002 0.0083 0.0028 0.0037 0.0055 0.0008 0.0031 0.0022 0.0064 0.0075
Nucleolus 0.0027 0.0001 0.0005 0.0001 0.0004 0.0006 0.0019 0.0001 0.0002 0.0003 0.0001 0.0003 0.0003 0.0002 0.0002 0 0.0002 0.0003
Nucleus 0.0198 0.0152 0.0133 0.0138 0.0084 0.0105 0.0078 0.0055 0.0148 0.0242 0.0078 0.0163 0.0119 0.0049 0.0069 0.011 0.0138 0.01
Peroxisomes 0.0091 0.0002 0.0026 0.0203 0.0133 0.0023 0.0483 0.0004 0.0141 0.0823 0.004 0.0052 0.0039 0.0001 0.0113 0.0003 0.0094 0.0027
Punctate Nuclear 0.0173 0.0019 0.0081 0.0055 0.0162 0.0042 0.0119 0.0017 0.0034 0.0132 0.0066 0.0045 0.0058 0.0003 0.0017 0.0013 0.0155 0.0018
Vacuole 0.0041 0.0012 0.0036 0.0017 0.0016 0.0016 0.002 0.0006 0.0015 0.0015 0.0008 0.0008 0.0017 0.0007 0.0027 0.0006 0.0026 0.0009
Vacuole Periphery 0.0023 0.0001 0.001 0.0005 0.001 0.0005 0.0012 0.0001 0.0003 0.0005 0.0003 0.0005 0.0005 0.0001 0.0005 0.0001 0.0007 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9198 26.4997 13.8376 24.7088 15.6584 21.9461 20.0953 23.1073 30.0672 23.9903
Translational Efficiency 1.9469 1.1535 2.0452 1.2507 1.6244 1.3846 1.6874 1.3156 1.0835 1.3478

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1274 894 677 196 1303 1855 254 1279 2577 2749 931 1475

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 773.76 903.23 934.06 1204.26 797.35 876.27 1226.06 1079.32 785.69 885.04 1013.72 1095.92
Standard Deviation 102.90 147.51 126.55 133.18 111.97 121.78 144.10 150.15 108.23 131.31 185.00 153.96
Intensity Change Log 2 — 0.223207 0.271629 0.638189 — 0.136162 0.620745 0.436838 — 0.179688 0.459327 0.539513

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000211 0.000290 0.000091 0.000731 0.000217 0.000198 0.000625 0.001119 0.000214 0.000228 0.000236 0.001067
Bud Neck 0.004679 0.002452 0.001551 0.002294 0.001792 0.003842 0.002073 0.004571 0.003219 0.003390 0.001693 0.004269
Bud Site 0.004588 0.008094 0.003964 0.007215 0.008820 0.008082 0.005829 0.028555 0.006728 0.008086 0.004473 0.025719
Cell Periphery 0.000109 0.000102 0.000037 0.000211 0.000220 0.000058 0.000206 0.000133 0.000165 0.000073 0.000083 0.000144
Cytoplasm 0.658460* 0.721776* 0.684618* 0.363439* 0.710591* 0.737649* 0.472017* 0.611842* 0.684819* 0.732487* 0.626615* 0.578834*
Cytoplasmic Foci 0.145977 0.102870 0.004105 0.018508 0.102022 0.085347 0.018693 0.024826 0.123753 0.091045 0.008085 0.023987
Eisosomes 0.000061 0.000028 0.000003 0.000065 0.000039 0.000023 0.000047 0.000018 0.000050 0.000024 0.000015 0.000025
Endoplasmic Reticulum 0.001109 0.001160 0.000434 0.004975 0.002337 0.000600 0.004942 0.001220 0.001730 0.000782 0.001664 0.001719
Endosome 0.008998 0.009964 0.000170 0.007615 0.005683 0.005014 0.006032 0.012682 0.007322 0.006624 0.001769 0.012009
Golgi 0.001470 0.001337 0.000043 0.000235 0.000900 0.002649 0.000225 0.013856 0.001182 0.002223 0.000093 0.012046
Lipid Particles 0.003079 0.002844 0.000181 0.003094 0.002344 0.001058 0.002145 0.001178 0.002707 0.001639 0.000716 0.001432
Mitochondria 0.000813 0.000837 0.000303 0.000163 0.002465 0.001278 0.000297 0.003748 0.001648 0.001134 0.000301 0.003272
Mitotic Spindle 0.000917 0.004066 0.002261 0.002143 0.001074 0.007709 0.005098 0.027053 0.000996 0.006524 0.003035 0.023743
None 0.004867 0.005443 0.005783 0.001121 0.005401 0.004166 0.004475 0.009316 0.005137 0.004581 0.005427 0.008227
Nuclear Periphery 0.000237 0.000240 0.000357 0.001410 0.000446 0.000186 0.001847 0.000731 0.000342 0.000203 0.000763 0.000822
Nuclear Periphery Foci 0.001841 0.003389 0.000427 0.017013 0.002797 0.001472 0.010552 0.004207 0.002325 0.002095 0.003189 0.005909
Nucleolus 0.000797 0.000792 0.000087 0.000208 0.000665 0.000661 0.000258 0.000221 0.000730 0.000704 0.000133 0.000220
Nucleus 0.078199 0.069656 0.274915* 0.148774* 0.098525 0.083642 0.276779* 0.156151* 0.088476 0.079093 0.275423* 0.155170*
Peroxisomes 0.001345 0.001514 0.000034 0.000288 0.000993 0.002054 0.000223 0.003290 0.001167 0.001879 0.000086 0.002891
Vacuole 0.081415 0.062482 0.020563 0.420201* 0.051207 0.053679 0.187391 0.088988 0.066141 0.056542 0.066078 0.133000
Vacuole Periphery 0.000827 0.000665 0.000074 0.000295 0.001461 0.000632 0.000246 0.006293 0.001148 0.000643 0.000121 0.005496

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.08 1.58 -4.90 -4.13 -6.61 0.38 -6.25 -4.45 -4.83 1.19 -0.33 -2.54 -5.19 -5.18 -4.02
Bud Neck 2.32 3.21 2.55 0.12 -1.75 -3.73 -3.49 -3.82 0.06 -0.62 -0.47 1.79 -0.80 -0.40 -2.91
Bud Site -3.06 0.68 -3.75 0.84 -3.22 0.32 0.88 -2.17 -3.03 -3.66 -1.44 1.73 -3.69 -2.95 -5.04
Cell Periphery 0.70 7.13 -4.33 -4.83 -8.46 3.29 0.21 2.31 -6.38 6.46 3.66 2.93 1.87 -6.29 -3.21
Cytoplasm -5.52 1.84 18.92 21.58 17.17 -2.89 22.08 19.53 23.17 -10.34 -6.85 13.02 22.42 27.95 5.64
Cytoplasmic Foci 6.67 31.91 28.90 19.23 -6.44 3.72 23.62 23.25 21.52 -2.16 8.64 40.66 35.41 27.71 -7.56
Eisosomes 4.62 8.29 0.08 -6.64 -12.60 6.48 -4.73 6.24 -1.06 9.50 6.95 8.03 5.74 -2.95 -5.36
Endoplasmic Reticulum -0.31 3.61 -5.98 -5.83 -7.22 2.79 -4.64 1.66 -7.61 9.04 2.91 -1.07 0.23 -8.12 2.43
Endosome -0.65 14.85 5.01 3.77 -11.45 1.47 1.12 2.39 1.25 1.54 1.46 15.45 4.73 3.18 -4.07
Golgi 0.31 3.88 3.46 3.37 -9.23 -2.64 3.69 -2.39 -0.63 -3.20 -2.10 5.38 -1.95 -0.77 -3.35
Lipid Particles 0.32 9.50 0.61 -0.12 -12.54 5.05 -0.59 3.35 -2.66 4.32 2.84 9.72 4.71 -0.44 -6.91
Mitochondria -0.01 2.48 3.41 1.82 5.01 1.28 2.31 1.32 0.09 -2.89 1.04 2.78 1.08 0.10 -2.91
Mitotic Spindle -2.43 -2.20 -3.28 0.73 -0.10 -4.22 -2.34 -7.41 -3.89 -2.74 -4.88 -3.20 -7.60 -4.26 -5.20
None -0.45 -1.03 2.16 2.54 3.94 1.39 2.04 -1.84 -3.54 -4.50 0.90 -0.01 -1.93 -2.85 -2.30
Nuclear Periphery -0.07 -7.80 -14.15 -13.17 -10.44 2.06 -10.55 -5.97 -16.42 7.68 2.07 -9.12 -11.55 -18.69 -2.01
Nuclear Periphery Foci -3.87 7.00 -8.76 -7.28 -9.79 2.55 -7.43 -1.85 -3.70 5.86 0.64 -2.42 -5.53 -5.33 -3.49
Nucleolus -0.43 5.95 4.74 2.74 -2.65 -0.33 3.29 3.01 2.97 -0.83 -0.38 7.01 5.08 3.89 -5.22
Nucleus 1.66 -25.95 -4.82 -5.35 11.52 3.29 -12.18 -15.39 -19.25 6.54 2.75 -27.59 -18.74 -20.73 13.70
Peroxisomes -0.82 8.91 7.63 5.80 -7.94 -2.01 2.64 -0.57 1.39 -3.32 -2.25 6.15 0.01 1.74 -3.74
Vacuole 3.42 8.31 -19.48 -20.30 -21.51 -1.51 -14.06 -22.31 -21.77 4.59 2.76 -6.26 -24.90 -26.25 -14.41
Vacuole Periphery 1.01 5.75 4.76 4.22 -5.68 1.69 2.54 -0.09 -1.61 -2.36 1.95 4.05 -0.37 -1.48 -2.57
Standard name
Human Ortholog
Description Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Det1

Det1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Det1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available