Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; required for vacuolar acidification; localizes to the matrix side of the inner mitochondrial membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.07 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.68 0.82 0.9 0.84 0.86 0.68 0.63 0.63 0.57 0.63 0.53 0.53 0.95 0.91 0.91 – 0.83 – 0.26 0.3 0.36 0.37 0.32 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0.05 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0.08 0.07 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.37 0.1 0 0.05 0 0.31 0.4 0.32 0.49 0.36 0.58 0.49 0 0 0 – 0.05 – 0.41 0.37 0.39 0.41 0.38 0.27
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0.05 0.07 0.1 0.08 0.07 0.1 0.14 0.07 0.09 0.05 0.09 0 0.07 0.06 – 0.09 – 0.05 0.08 0.09 0.06 0.09 0.14
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 2 0 1 1 0 0 0 1 0 0 1 1 0 – 1 – 2 14 7 2 22 21
Bud 0 9 5 3 5 15 20 14 29 12 29 19 0 2 0 – 5 – 2 7 0 1 9 11
Bud Neck 0 1 14 15 19 16 17 17 10 8 6 18 3 7 8 – 4 – 1 4 1 1 11 12
Bud Site 0 0 0 1 0 1 6 3 4 3 2 6 0 0 0 – 0 – – – – – – –
Cell Periphery 0 2 1 2 2 2 2 0 0 1 2 3 2 0 3 – 5 – 0 2 2 1 9 6
Cytoplasm 62 258 169 256 351 250 330 235 283 294 302 292 245 366 377 – 161 – 34 116 71 68 183 150
Endoplasmic Reticulum 1 1 1 0 0 0 2 0 2 0 0 1 1 2 2 – 10 – 6 1 0 0 3 1
Endosome 0 15 0 0 2 2 3 1 1 0 1 0 2 3 10 – 5 – 10 26 6 6 26 28
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 – 0 – 0 3 2 1 2 0
Mitochondria 34 31 0 16 15 113 211 120 243 167 327 268 2 0 0 – 9 – 55 145 77 75 220 118
Nucleus 1 0 0 1 1 2 6 2 1 2 3 1 0 0 0 – 1 – 0 1 0 0 0 0
Nuclear Periphery 3 0 0 0 1 0 0 0 1 2 0 1 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 1 2 1 0 0 0 – 1 – 0 1 0 0 0 0
Peroxisomes 2 1 0 1 0 1 0 1 1 0 0 2 1 0 6 – 0 – 1 1 2 0 7 4
SpindlePole 1 16 13 31 34 26 53 54 35 40 28 51 9 29 26 – 18 – 6 30 17 11 51 63
Vac/Vac Membrane 0 5 0 2 2 5 1 4 5 4 2 5 0 1 2 – 5 – 4 19 2 4 18 10
Unique Cell Count 91 316 188 306 406 366 525 374 496 465 567 547 259 403 415 195 133 388 200 183 581 442
Labelled Cell Count 104 343 205 328 433 434 652 451 615 535 704 668 266 411 436 225 133 388 200 183 581 442


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.1 4.5 4.2 4.6 3.6 3.3 4.1 3.1 3.6 2.8 3.2 4.8 4.9 4.9 4.5 5.0 4.7 4.3 4.8 4.9
Std Deviation (1e-4) 0.9 1.0 1.2 1.3 1.8 1.3 1.5 2.0 1.4 1.3 1.1 0.6 1.1 1.3 1.6 1.0 1.3 1.4 1.4 1.7 1.7
Intensity Change (Log2) – – – -0.07 0.04 -0.33 -0.42 -0.12 -0.51 -0.33 -0.65 -0.46 0.11 0.13 0.13 0.01 0.16 0.09 -0.05 0.1 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6055 0.6069 0.5784 0.1015 0.1582 0.7152 -0.7251 -0.533 -0.4275 -0.2786 -0.4852 -0.4463 -1.7068 -1.3166 -1.0954 -0.8525 -0.5067 -1.4955
Actin 0.032 0.0026 0.0042 0.0013 0.0295 0.0087 0.0188 0.0192 0.0171 0.007 0.0138 0.0049 0.001 0.0066 0.0151 0.0093 0.0195 0.0013
Bud 0.0016 0.005 0.0013 0.0064 0.0017 0.0029 0.001 0.003 0.005 0.0016 0.004 0.0008 0.0003 0.0009 0.0013 0.0007 0.0005 0.0002
Bud Neck 0.0029 0.0008 0.0011 0.0025 0.0019 0.0071 0.0042 0.0013 0.0032 0.0012 0.0003 0.0094 0.001 0.0007 0.0007 0.0007 0.0016 0.0214
Bud Periphery 0.0043 0.0054 0.0039 0.0057 0.0051 0.0048 0.0032 0.0078 0.0052 0.0113 0.0114 0.0021 0.0007 0.0016 0.0041 0.0018 0.0009 0.0004
Bud Site 0.0034 0.0127 0.0042 0.0105 0.0022 0.0008 0.0091 0.0189 0.0122 0.0014 0.0014 0.0011 0.0023 0.01 0.0043 0.0009 0.0011 0.0003
Cell Periphery 0.0008 0.0011 0.002 0.0018 0.0009 0.0003 0.0012 0.002 0.0011 0.001 0.0004 0.0005 0.0017 0.0013 0.0007 0.0004 0.0004 0.0003
Cytoplasm 0.0615 0.1236 0.0899 0.0976 0.0909 0.1162 0.1 0.1256 0.0891 0.0809 0.1076 0.1462 0.112 0.1613 0.0874 0.08 0.0274 0.2269
Cytoplasmic Foci 0.0894 0.0755 0.0708 0.0574 0.0904 0.0731 0.1125 0.1237 0.0764 0.0481 0.0869 0.1155 0.1227 0.0888 0.048 0.0758 0.0687 0.0564
Eisosomes 0.0011 0.0006 0.0013 0.0005 0.0017 0.0003 0.0007 0.0005 0.0007 0.0005 0.0002 0.0003 0.0006 0.0015 0.0012 0.0007 0.0004 0.0002
Endoplasmic Reticulum 0.0057 0.0027 0.0036 0.001 0.0024 0.0018 0.0043 0.0022 0.0024 0.0018 0.0023 0.0026 0.003 0.0055 0.0047 0.0028 0.0012 0.0038
Endosome 0.049 0.0431 0.0339 0.0269 0.0597 0.0263 0.062 0.0722 0.0312 0.027 0.0821 0.0388 0.093 0.0409 0.0822 0.0878 0.0462 0.0774
Golgi 0.0092 0.0074 0.008 0.0108 0.0116 0.0058 0.0118 0.0165 0.0092 0.0087 0.0137 0.0062 0.0161 0.0093 0.0148 0.0307 0.0674 0.0141
Lipid Particles 0.0298 0.0084 0.007 0.0018 0.0091 0.0109 0.023 0.0078 0.0109 0.03 0.016 0.0105 0.029 0.0181 0.0102 0.0136 0.0379 0.0136
Mitochondria 0.0342 0.0147 0.0457 0.0406 0.0282 0.0258 0.0513 0.0675 0.0463 0.0463 0.0996 0.0442 0.0247 0.016 0.0604 0.1139 0.0174 0.0639
None 0.6219 0.6714 0.6909 0.6933 0.6258 0.6807 0.5454 0.4944 0.6557 0.697 0.5027 0.5787 0.5554 0.6186 0.6128 0.5657 0.6257 0.5039
Nuclear Periphery 0.0073 0.0017 0.0012 0.0005 0.0075 0.0009 0.0098 0.0007 0.0015 0.0009 0.0085 0.0009 0.0009 0.0011 0.0126 0.001 0.0013 0.0014
Nucleolus 0.0016 0.0014 0.0014 0.0063 0.0011 0.0007 0.0018 0.0013 0.0015 0.001 0.0005 0.0009 0.0029 0.0006 0.0007 0.0003 0.0003 0.0003
Nucleus 0.004 0.0029 0.0031 0.0088 0.0016 0.001 0.0031 0.0018 0.0031 0.0016 0.0022 0.0043 0.0016 0.0008 0.002 0.0006 0.0004 0.0012
Peroxisomes 0.0257 0.0097 0.0198 0.0158 0.0149 0.026 0.018 0.0232 0.0194 0.0247 0.0287 0.0227 0.0094 0.0088 0.0192 0.007 0.0768 0.0047
Punctate Nuclear 0.0082 0.0028 0.0024 0.0055 0.0056 0.0032 0.01 0.0014 0.0025 0.002 0.006 0.0039 0.0033 0.0008 0.0022 0.0007 0.0017 0.0008
Vacuole 0.0047 0.0056 0.0035 0.0042 0.0063 0.002 0.0065 0.0073 0.0049 0.0045 0.0054 0.0041 0.0163 0.0055 0.0101 0.0033 0.0019 0.0049
Vacuole Periphery 0.0018 0.0011 0.001 0.0009 0.0021 0.0007 0.0024 0.0016 0.0014 0.0015 0.0061 0.0016 0.002 0.0012 0.005 0.0021 0.0011 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
52 287 2540 1273 923 1626 321 1486 975 1913 2861 2759

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.59 716.66 787.53 760.34 580.16 667.31 871.51 838.00 586.58 674.71 796.95 802.17
Standard Deviation 159.16 84.71 84.69 104.26 82.27 84.36 96.39 116.78 92.15 86.23 90.07 117.73
Intensity Change Log 2 — 0.032718 0.168764 0.118074 — 0.201906 0.587066 0.530499 — 0.111824 0.373360 0.319585

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000726 0.001277 0.000233 0.008084 0.000227 0.000822 0.000824 0.001199 0.000254 0.000890 0.000299 0.004376
Bud Neck 0.047766 0.069576 0.023226 0.029268 0.026271 0.060978 0.008809 0.025394 0.027418 0.062268 0.021609 0.027181
Bud Site 0.070135 0.036522 0.013201 0.077304 0.007030 0.027019 0.006809 0.067734 0.010396 0.028444 0.012484 0.072150
Cell Periphery 0.001872 0.000369 0.000097 0.000331 0.000204 0.000148 0.000277 0.000158 0.000293 0.000182 0.000118 0.000238
Cytoplasm 0.278135* 0.144997* 0.343063* 0.303305* 0.293456* 0.241419* 0.215435* 0.408955* 0.292638* 0.226953* 0.328743* 0.360208*
Cytoplasmic Foci 0.206515* 0.357859* 0.061493 0.106821 0.352403* 0.326686* 0.132592 0.057296 0.344622* 0.331363* 0.069470 0.080147
Eisosomes 0.000222 0.000259 0.000049 0.000192 0.000247 0.000203 0.000205 0.000068 0.000245 0.000212 0.000066 0.000125
Endoplasmic Reticulum 0.010376 0.000682 0.002428 0.006356 0.001462 0.000971 0.004711 0.001445 0.001937 0.000928 0.002684 0.003711
Endosome 0.024912 0.068539 0.010485 0.012465 0.039123 0.038954 0.019725 0.015627 0.038366 0.043392 0.011522 0.014168
Golgi 0.008660 0.028882 0.000784 0.004955 0.011307 0.014189 0.002097 0.004931 0.011165 0.016393 0.000931 0.004942
Lipid Particles 0.035477 0.009191 0.004965 0.004894 0.030538 0.013982 0.013635 0.001928 0.030802 0.013263 0.005938 0.003297
Mitochondria 0.030431 0.036627 0.006679 0.008621 0.032600 0.053347 0.001694 0.007470 0.032484 0.050838 0.006120 0.008001
Mitotic Spindle 0.028979 0.008137 0.012504 0.037686 0.003697 0.008445 0.001272 0.062240 0.005045 0.008399 0.011244 0.050911
None 0.010255 0.000778 0.003622 0.001611 0.007101 0.001970 0.004141 0.004234 0.007269 0.001791 0.003681 0.003024
Nuclear Periphery 0.001742 0.000227 0.005188 0.001322 0.001002 0.000489 0.002214 0.001403 0.001041 0.000450 0.004854 0.001366
Nuclear Periphery Foci 0.003356 0.000240 0.000843 0.001953 0.000645 0.000387 0.003178 0.002064 0.000789 0.000364 0.001105 0.002013
Nucleolus 0.018076 0.001898 0.004731 0.001038 0.005648 0.002869 0.004059 0.000994 0.006310 0.002723 0.004656 0.001014
Nucleus 0.137491* 0.058768 0.441107* 0.184516* 0.119924 0.086303 0.450486* 0.174389* 0.120860 0.082172 0.442159* 0.179062*
Peroxisomes 0.031345 0.111178* 0.006765 0.052204 0.024767 0.052831 0.014383 0.021007 0.025118 0.061585 0.007619 0.035401
Vacuole 0.040567 0.060990 0.057062 0.155451 0.038214 0.063716 0.112580 0.138577 0.038339 0.063307 0.063291 0.146363
Vacuole Periphery 0.012962 0.003003 0.001476 0.001622 0.004137 0.004272 0.000875 0.002889 0.004608 0.004081 0.001409 0.002304

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 2.34 -6.59 -5.65 -7.39 -4.73 -3.72 -7.27 -0.58 0.10 -5.46 -1.94 -8.23 -6.19 -7.99
Bud Neck -1.20 1.33 1.22 5.67 -0.79 -9.29 6.18 3.12 12.85 -4.10 -9.67 1.83 2.15 13.35 0.48
Bud Site 1.50 2.62 0.47 -3.80 -11.63 -9.78 -0.03 -10.41 -3.63 -9.63 -7.93 -0.69 -13.64 -6.90 -13.71
Cell Periphery 1.41 1.68 1.51 0.70 -10.59 3.66 -3.92 5.99 3.83 9.58 1.83 3.03 1.80 -0.32 -7.01
Cytoplasm 3.20 -1.37 -0.16 -9.86 5.31 4.95 6.26 -5.66 -12.14 -11.29 6.51 -2.32 -3.06 -12.56 -1.04
Cytoplasmic Foci -3.85 4.34 3.59 19.73 -5.92 2.64 18.53 38.03 44.77 8.07 1.49 34.32 35.31 46.10 1.52
Eisosomes -0.77 5.57 1.64 2.58 -11.11 2.00 1.11 9.30 12.65 11.80 1.64 9.57 6.91 8.49 -7.86
Endoplasmic Reticulum 1.96 1.54 0.84 -11.36 -7.17 1.86 -7.49 -2.29 -7.77 6.98 2.76 -3.22 -4.90 -12.65 -3.32
Endosome -5.39 3.19 3.35 8.72 0.76 0.02 4.64 10.24 13.82 4.06 -1.51 9.69 10.61 16.53 2.62
Golgi -3.84 2.55 1.79 6.27 -5.01 -1.84 7.86 7.58 10.22 -0.73 -3.36 9.88 7.81 12.04 -4.98
Lipid Particles 2.32 2.68 2.71 3.34 0.89 7.15 6.85 13.61 12.28 8.34 7.83 11.89 13.28 11.68 8.63
Mitochondria -0.38 1.74 1.77 5.73 0.43 -4.75 10.66 9.53 14.84 -2.97 -4.50 9.28 9.50 15.69 1.26
Mitotic Spindle 1.51 1.10 -0.49 -6.97 -5.72 -2.63 1.65 -10.26 -9.12 -11.37 -1.89 -3.77 -11.91 -11.30 -9.79
None 2.71 1.93 2.48 -4.34 5.54 4.44 1.94 2.81 -2.88 0.42 4.96 3.39 4.12 -2.36 1.99
Nuclear Periphery 2.31 -5.51 -0.18 -12.14 12.15 3.31 -5.37 -7.70 -14.21 -0.55 3.96 -14.56 -6.92 -17.15 11.32
Nuclear Periphery Foci 2.24 1.72 0.94 -6.04 -3.49 2.97 -6.55 -1.43 -2.05 3.74 3.86 -3.12 -2.64 -4.32 -1.23
Nucleolus 1.80 1.40 1.90 1.69 8.82 2.98 1.96 5.44 5.33 4.17 3.60 1.18 5.58 5.22 10.55
Nucleus 2.72 -10.86 -2.26 -14.47 27.87 5.10 -17.56 -12.07 -18.59 11.79 6.08 -41.22 -12.94 -23.35 32.24
Peroxisomes -6.29 2.83 -0.63 7.67 -9.38 -8.65 3.76 5.27 13.57 0.62 -11.21 8.78 0.68 12.12 -8.61
Vacuole -2.37 -3.45 -18.43 -17.05 -22.34 -7.77 -10.80 -27.87 -23.60 -6.81 -8.16 -12.14 -37.02 -31.06 -28.88
Vacuole Periphery 1.84 2.15 2.20 4.00 1.06 -0.22 7.17 5.36 5.94 -2.32 0.83 6.17 6.21 7.14 0.14
Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; required for vacuolar acidification; localizes to the matrix side of the inner mitochondrial membrane
Localization
Cell Percentages mitochondrion (17%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Rrg1

Rrg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available