Standard name | |
---|---|
Human Ortholog | |
Description | Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 6 | 23 | 28 | 19 | 30 | 25 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 6 | 10 | 13 | 20 | 40 | 25 | 25 | 40 | 31 | 40 | 4 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 190 | 206 | 395 | 306 | 304 | 289 | 402 | 372 | 237 | 273 | 174 | 169 | 194 | 146 | 181 | 48 | 69 | 40 | 185 | 200 | 395 | 101 | 126 | 152 |
Endoplasmic Reticulum | 3 | 4 | 16 | 3 | 27 | 23 | 75 | 61 | 48 | 57 | 36 | 37 | 6 | 3 | 2 | 3 | 6 | 2 | 2 | 0 | 15 | 3 | 11 | 15 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 | 2 | 5 | 5 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 6 | 12 | 21 | 38 | 56 | 55 | 80 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 6 | 0 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 3 | 8 | 0 | 0 | 1 |
Unique Cell Count | 194 | 208 | 409 | 311 | 334 | 326 | 507 | 483 | 323 | 394 | 265 | 297 | 200 | 150 | 184 | 48 | 73 | 41 | 193 | 209 | 433 | 110 | 145 | 180 |
Labelled Cell Count | 199 | 213 | 421 | 323 | 349 | 347 | 556 | 520 | 371 | 466 | 329 | 376 | 204 | 150 | 189 | 53 | 81 | 43 | 193 | 209 | 433 | 110 | 145 | 180 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 71.3 | 56.5 | 38.3 | 33.6 | 30.9 | 26.4 | 23.4 | 21.7 | 19.9 | 18.9 | 17.5 | 16.6 | 59.8 | 53.3 | 46.7 | 76.5 | 75.2 | 77.8 | 59.8 | 65.2 | 65.6 |
Std Deviation (1e-4) | 16.2 | 13.9 | 8.3 | 8.0 | 6.8 | 6.1 | 5.1 | 4.9 | 4.7 | 4.5 | 4.3 | 4.2 | 12.9 | 13.3 | 12.2 | 24.6 | 21.2 | 27.0 | 19.6 | 20.6 | 20.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.31 | -0.53 | -0.71 | -0.82 | -0.94 | -1.02 | -1.13 | -1.2 | 0.64 | 0.48 | 0.29 | 1.0 | 0.98 | 1.02 | 0.64 | 0.77 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 4.4 | 4.9 | 5.0 | 5.7 | 6.3 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.6 | 2.1 | 3.4 | 4.4 | 3.0 | 4.2 | 5.3 | 5.7 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | -3.2 | -4.2 | -7.7 | -8.4 | -9.1 | -10.3 | -10.8 | -13.0 | 0.3 | 0.4 | 1.2 | 1.3 | -0.9 | 0.3 |
Endoplasmic Reticulum | -2.4 | 2.4 | 1.9 | 5.5 | 4.6 | 5.2 | 5.2 | 4.6 | 4.3 | -0.6 | -1.1 | -1.9 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.1 | 4.3 | 7.1 | 7.9 | 9.6 | 11.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 48.8142 | 68.6527 | 64.5215 | 66.3663 | 54.8954 | 62.6437 | 50.6407 | 69.5537 | 65.3652 | 63.492 | 64.2093 | 62.9937 | 58.4381 | 57.2452 | 63.8025 | 56.1274 | 59.549 | 62.8418 |
Actin | 0.0051 | 0.0002 | 0.0003 | 0.0001 | 0.0011 | 0.0003 | 0.0025 | 0.001 | 0.0002 | 0.0004 | 0.0023 | 0.0003 | 0.0044 | 0.0006 | 0.0011 | 0.0007 | 0.0008 | 0.0027 |
Bud | 0.0011 | 0.0005 | 0.0006 | 0.001 | 0.001 | 0.0007 | 0.0009 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0005 | 0.0007 | 0.0008 | 0.0018 | 0.0021 | 0.0004 | 0.0035 |
Bud Neck | 0.0011 | 0.001 | 0.0008 | 0.0007 | 0.0015 | 0.002 | 0.0015 | 0.0005 | 0.0005 | 0.0008 | 0.0005 | 0.0015 | 0.0007 | 0.0004 | 0.0007 | 0.0005 | 0.001 | 0.0025 |
Bud Periphery | 0.0019 | 0.0005 | 0.0006 | 0.0006 | 0.0026 | 0.0008 | 0.0017 | 0.0005 | 0.0004 | 0.0008 | 0.0002 | 0.0009 | 0.0008 | 0.0003 | 0.0007 | 0.0012 | 0.0002 | 0.0028 |
Bud Site | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0016 | 0.0001 | 0.0004 |
Cell Periphery | 0.0179 | 0.0168 | 0.0147 | 0.0118 | 0.0167 | 0.0058 | 0.0222 | 0.0121 | 0.0103 | 0.0138 | 0.0058 | 0.0052 | 0.0063 | 0.0009 | 0.0011 | 0.0035 | 0.0003 | 0.0022 |
Cytoplasm | 0.5 | 0.8136 | 0.7228 | 0.6802 | 0.6798 | 0.7057 | 0.6657 | 0.8862 | 0.8506 | 0.7122 | 0.7833 | 0.8376 | 0.7779 | 0.9556 | 0.9133 | 0.7391 | 0.9178 | 0.8133 |
Cytoplasmic Foci | 0.0076 | 0.0007 | 0.0038 | 0.0011 | 0.0095 | 0.0058 | 0.005 | 0.0008 | 0.0029 | 0.0019 | 0.0015 | 0.0028 | 0.0047 | 0.0026 | 0.0047 | 0.0034 | 0.0063 | 0.0067 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.1399 | 0.0376 | 0.0616 | 0.009 | 0.0597 | 0.133 | 0.1049 | 0.0295 | 0.0404 | 0.027 | 0.0135 | 0.0732 | 0.0763 | 0.0169 | 0.0186 | 0.0052 | 0.0175 | 0.0526 |
Endosome | 0.013 | 0.002 | 0.0052 | 0.0073 | 0.0093 | 0.0118 | 0.0076 | 0.0022 | 0.0043 | 0.0066 | 0.013 | 0.0109 | 0.0125 | 0.0039 | 0.01 | 0.0165 | 0.0139 | 0.0287 |
Golgi | 0.0015 | 0.0002 | 0.0003 | 0.0001 | 0.0009 | 0.001 | 0.0015 | 0.0011 | 0.0005 | 0.0005 | 0.0019 | 0.0013 | 0.0014 | 0.0011 | 0.001 | 0.0004 | 0.0013 | 0.0055 |
Lipid Particles | 0.0042 | 0.0009 | 0.0023 | 0.0004 | 0.0055 | 0.0019 | 0.0054 | 0.0015 | 0.0018 | 0.0022 | 0.0026 | 0.0005 | 0.0019 | 0.0001 | 0.0013 | 0.0007 | 0.0001 | 0.0002 |
Mitochondria | 0.0078 | 0.0003 | 0.0004 | 0.0003 | 0.009 | 0.0047 | 0.0089 | 0.0012 | 0.0013 | 0.0003 | 0.0016 | 0.0017 | 0.0025 | 0.0008 | 0.0006 | 0.005 | 0.0005 | 0.0079 |
None | 0.0045 | 0.0001 | 0.0011 | 0.0001 | 0.0055 | 0.0019 | 0.0048 | 0.0003 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0035 | 0.0002 | 0.0003 | 0.0033 | 0.0003 | 0.0005 |
Nuclear Periphery | 0.0459 | 0.009 | 0.0114 | 0.0206 | 0.0143 | 0.0142 | 0.0195 | 0.0032 | 0.0044 | 0.0067 | 0.0181 | 0.0075 | 0.0121 | 0.0015 | 0.004 | 0.0071 | 0.0053 | 0.0086 |
Nucleolus | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0.0024 | 0.0011 | 0.0069 | 0.0001 | 0.0009 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0.0033 | 0 | 0.0005 |
Nucleus | 0.0139 | 0.0031 | 0.003 | 0.0058 | 0.0058 | 0.0039 | 0.0093 | 0.0015 | 0.0023 | 0.003 | 0.0036 | 0.0018 | 0.0034 | 0.0012 | 0.0016 | 0.0061 | 0.0017 | 0.0043 |
Peroxisomes | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0014 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0048 | 0 | 0.001 | 0 | 0.0009 | 0.0002 | 0.0018 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.005 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Vacuole | 0.1754 | 0.1047 | 0.1488 | 0.206 | 0.1353 | 0.0844 | 0.1105 | 0.0542 | 0.0706 | 0.2064 | 0.1223 | 0.0446 | 0.0703 | 0.0108 | 0.0316 | 0.1429 | 0.0238 | 0.0401 |
Vacuole Periphery | 0.0507 | 0.0081 | 0.0205 | 0.0544 | 0.0374 | 0.0204 | 0.016 | 0.0031 | 0.0059 | 0.0158 | 0.0277 | 0.0092 | 0.0137 | 0.0017 | 0.0071 | 0.0568 | 0.0084 | 0.0168 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 264.7584 | 309.4513 | 299.6817 | 225.3595 | 255.3136 | 273.0413 | 232.2615 | 213.8614 | 229.2232 | 203.7688 |
Translational Efficiency | 2.026 | 1.9246 | 1.5184 | 2.3683 | 1.7554 | 1.5116 | 1.5482 | 1.6834 | 1.3272 | 1.5556 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1439 | 722 | 237 | 792 | 2153 | 1520 | 99 | 1756 | 3592 | 2242 | 336 | 2548 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3504.00 | 4103.36 | 6160.64 | 4872.94 | 3489.79 | 3632.60 | 5873.79 | 4690.53 | 3495.48 | 3784.20 | 6076.12 | 4747.23 |
Standard Deviation | 719.37 | 840.91 | 928.86 | 1428.42 | 638.41 | 821.08 | 1151.48 | 1053.49 | 672.05 | 856.25 | 1008.14 | 1185.84 |
Intensity Change Log 2 | — | 0.227803 | 0.814077 | 0.475790 | — | 0.057862 | 0.751151 | 0.426611 | — | 0.145506 | 0.783021 | 0.451460 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000109 | 0.000155 | 0.000150 | 0.000313 | 0.000039 | 0.000081 | 0.000247 | 0.000128 | 0.000067 | 0.000105 | 0.000178 | 0.000186 |
Bud Neck | 0.000333 | 0.001046 | 0.003629 | 0.004909 | 0.000155 | 0.000389 | 0.010620 | 0.001070 | 0.000226 | 0.000601 | 0.005689 | 0.002263 |
Bud Site | 0.004186 | 0.005857 | 0.002751 | 0.017197 | 0.002518 | 0.003082 | 0.003392 | 0.003618 | 0.003186 | 0.003975 | 0.002940 | 0.007839 |
Cell Periphery | 0.012334 | 0.013669 | 0.024306 | 0.006056 | 0.004839 | 0.007250 | 0.030187 | 0.014622 | 0.007841 | 0.009317 | 0.026039 | 0.011960 |
Cytoplasm | 0.674234* | 0.644563* | 0.373822* | 0.399796* | 0.774987* | 0.826711* | 0.164533* | 0.627273* | 0.734624* | 0.768053* | 0.312157* | 0.556566* |
Cytoplasmic Foci | 0.012818 | 0.015364 | 0.023084 | 0.011824 | 0.019608 | 0.008058 | 0.026003 | 0.006384 | 0.016888 | 0.010411 | 0.023944 | 0.008074 |
Eisosomes | 0.000002 | 0.000003 | 0.000006 | 0.000008 | 0.000001 | 0.000001 | 0.000012 | 0.000002 | 0.000001 | 0.000002 | 0.000008 | 0.000004 |
Endoplasmic Reticulum | 0.001500 | 0.001217 | 0.000438 | 0.000416 | 0.002035 | 0.000680 | 0.000867 | 0.000684 | 0.001821 | 0.000853 | 0.000564 | 0.000601 |
Endosome | 0.001004 | 0.001760 | 0.001169 | 0.005750 | 0.001046 | 0.000738 | 0.002147 | 0.000872 | 0.001029 | 0.001067 | 0.001457 | 0.002388 |
Golgi | 0.096033 | 0.114014* | 0.105766 | 0.025459 | 0.063282 | 0.051183 | 0.070566 | 0.037882 | 0.076403 | 0.071417 | 0.095395 | 0.034021 |
Lipid Particles | 0.006731 | 0.019499 | 0.019119 | 0.014470 | 0.006353 | 0.008061 | 0.034082 | 0.015605 | 0.006504 | 0.011744 | 0.023528 | 0.015252 |
Mitochondria | 0.000314 | 0.000167 | 0.000190 | 0.001120 | 0.000328 | 0.000149 | 0.000174 | 0.000252 | 0.000322 | 0.000155 | 0.000185 | 0.000522 |
Mitotic Spindle | 0.001473 | 0.002474 | 0.003698 | 0.006632 | 0.000907 | 0.000772 | 0.004386 | 0.000677 | 0.001133 | 0.001320 | 0.003901 | 0.002528 |
None | 0.002366 | 0.001532 | 0.001837 | 0.002051 | 0.000986 | 0.002694 | 0.000688 | 0.000919 | 0.001539 | 0.002320 | 0.001498 | 0.001271 |
Nuclear Periphery | 0.000451 | 0.000880 | 0.004645 | 0.000661 | 0.000225 | 0.000274 | 0.005036 | 0.000532 | 0.000316 | 0.000469 | 0.004760 | 0.000572 |
Nuclear Periphery Foci | 0.010392 | 0.019191 | 0.032826 | 0.008791 | 0.008350 | 0.011309 | 0.034262 | 0.010422 | 0.009168 | 0.013847 | 0.033249 | 0.009915 |
Nucleolus | 0.000090 | 0.000111 | 0.000165 | 0.001125 | 0.000031 | 0.000049 | 0.000213 | 0.000097 | 0.000055 | 0.000069 | 0.000179 | 0.000417 |
Nucleus | 0.005997 | 0.006987 | 0.015381 | 0.026305 | 0.001446 | 0.004508 | 0.035848 | 0.007097 | 0.003269 | 0.005306 | 0.021411 | 0.013068 |
Peroxisomes | 0.000019 | 0.000020 | 0.000012 | 0.000401 | 0.000029 | 0.000018 | 0.000021 | 0.000035 | 0.000025 | 0.000018 | 0.000015 | 0.000149 |
Vacuole | 0.040628 | 0.076198 | 0.247348* | 0.409911* | 0.034773 | 0.038964 | 0.470087* | 0.214148* | 0.037119 | 0.050954 | 0.312976* | 0.274998* |
Vacuole Periphery | 0.128985* | 0.075292 | 0.139659* | 0.056806 | 0.078063 | 0.035031 | 0.106630* | 0.057680 | 0.098463 | 0.047996 | 0.129927* | 0.057408 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.20 | -2.35 | -3.92 | -2.88 | -2.63 | -2.87 | -5.87 | -2.64 | -1.70 | 2.17 | -3.09 | -6.61 | -3.92 | -2.77 | -0.06 |
Bud Neck | -5.90 | -7.34 | -9.30 | -8.25 | -3.68 | -4.41 | -7.16 | -10.10 | -7.28 | 6.52 | -6.97 | -9.58 | -11.59 | -9.91 | 4.87 |
Bud Site | -2.19 | 2.41 | -6.38 | -5.47 | -7.16 | -1.21 | -2.80 | -3.75 | -1.69 | 0.40 | -1.94 | -0.09 | -6.93 | -5.50 | -6.77 |
Cell Periphery | -1.07 | -5.25 | 7.77 | 7.64 | 8.67 | -3.96 | -6.84 | -11.76 | -7.62 | 3.78 | -2.45 | -9.46 | -5.66 | -3.01 | 7.24 |
Cytoplasm | 1.96 | 13.01 | 18.42 | 13.57 | -1.76 | -5.59 | 24.73 | 15.15 | 19.42 | -17.77 | -3.91 | 23.06 | 20.87 | 22.47 | -12.80 |
Cytoplasmic Foci | -2.21 | -4.70 | -0.66 | 1.14 | 4.00 | 10.63 | -1.93 | 12.58 | 1.87 | 3.78 | 7.72 | -3.79 | 9.72 | 2.20 | 6.73 |
Eisosomes | -3.28 | -6.09 | -3.51 | -2.83 | -0.38 | -1.62 | -7.61 | -5.07 | -2.59 | 6.61 | -2.70 | -9.69 | -4.97 | -3.67 | 5.03 |
Endoplasmic Reticulum | 1.20 | 5.08 | 5.61 | 6.12 | 1.04 | 6.45 | 4.77 | 6.00 | 0.10 | 0.13 | 6.10 | 8.31 | 7.59 | 2.41 | -0.49 |
Endosome | -5.67 | -1.66 | -3.75 | -2.90 | -3.48 | 3.65 | -3.73 | 2.02 | -1.45 | 4.26 | -0.62 | -3.67 | -3.26 | -3.08 | -1.53 |
Golgi | -2.62 | -1.12 | 18.83 | 16.44 | 14.30 | 3.44 | 0.55 | 9.47 | 4.87 | 3.05 | 1.55 | -2.76 | 18.92 | 14.25 | 12.34 |
Lipid Particles | -6.67 | -7.75 | -4.35 | 2.21 | 2.74 | -1.78 | -5.53 | -9.62 | -6.60 | 3.74 | -5.77 | -9.12 | -10.30 | -3.13 | 4.59 |
Mitochondria | 2.05 | 1.49 | -1.76 | -2.23 | -2.11 | 3.88 | 1.29 | 0.05 | -0.90 | -0.36 | 4.08 | 2.60 | -1.07 | -2.09 | -1.69 |
Mitotic Spindle | -1.81 | -3.81 | -2.59 | -2.01 | -1.22 | 1.19 | -2.94 | 2.46 | 0.57 | 3.09 | -0.88 | -5.84 | -2.16 | -1.86 | 2.55 |
None | 2.79 | 1.74 | 0.98 | -1.00 | -0.24 | -2.60 | 1.80 | 0.32 | 2.62 | -0.76 | -1.70 | 0.14 | 1.54 | 2.29 | 1.47 |
Nuclear Periphery | -1.93 | -8.11 | -1.95 | 0.66 | 7.59 | -1.84 | -6.57 | -9.94 | -7.17 | 6.10 | -1.99 | -10.53 | -5.17 | -1.82 | 9.85 |
Nuclear Periphery Foci | -5.60 | -7.39 | 1.40 | 6.47 | 7.88 | -3.05 | -4.67 | -3.19 | 0.88 | 4.24 | -5.53 | -9.09 | -1.15 | 4.73 | 8.82 |
Nucleolus | -0.60 | -4.41 | -3.14 | -3.05 | -2.87 | -3.81 | -4.46 | -14.97 | -8.13 | 3.19 | -1.15 | -7.52 | -3.58 | -3.40 | -1.97 |
Nucleus | -1.18 | -4.99 | -10.18 | -9.69 | -6.06 | -8.73 | -4.30 | -11.45 | -5.91 | 3.72 | -4.97 | -6.08 | -12.89 | -10.88 | 2.26 |
Peroxisomes | -0.24 | 1.38 | -2.24 | -2.23 | -2.28 | 2.65 | 0.69 | -0.96 | -2.74 | -1.40 | 2.01 | 2.97 | -2.34 | -2.50 | -2.54 |
Vacuole | -7.03 | -14.79 | -31.73 | -26.76 | -7.98 | -1.59 | -15.43 | -29.88 | -27.99 | 8.42 | -5.52 | -19.96 | -41.37 | -37.03 | 2.52 |
Vacuole Periphery | 7.59 | -0.73 | 14.37 | 5.52 | 10.13 | 12.79 | -0.75 | 6.47 | -7.44 | 2.78 | 14.87 | -3.08 | 14.60 | -1.63 | 9.63 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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