Standard name
Human Ortholog
Description Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.98 0.97 0.95 0.87 0.8 0.79 – 0.64 0.63 0.55 0.99 0.99 1.0 0.94 0.95 0.94 0.64 0.76 0.52 0.59 0.69 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.09 0.05 0.15 0 0.15 0.07 0 0.05
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.25 0.37 0.32 – 0.55 0.54 0.64 0 0 0 0.07 0 0 0.1 0.09 0.19 0.21 0.11 0.08
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 9 8 18 6 14 26
Bud 1 3 1 0 0 8 12 10 – 14 28 16 0 1 0 0 1 0 2 2 4 7 24 35
Bud Neck 2 2 5 0 2 0 4 7 – 2 2 2 6 4 3 1 2 2 4 4 7 2 14 19
Bud Site 0 0 0 0 0 1 0 3 – 2 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 5 4 0 3 3 – 0 1 2 3 0 2 1 1 0 3 5 8 9 28 19
Cytoplasm 385 384 520 310 385 498 505 523 – 391 350 315 658 406 796 221 285 181 306 324 291 279 693 667
Endoplasmic Reticulum 3 10 1 1 0 3 0 0 – 0 0 1 0 0 1 12 28 10 69 15 85 34 41 47
Endosome 4 1 0 3 8 5 3 1 – 1 0 3 1 1 0 2 4 4 4 4 7 4 4 8
Golgi 1 0 1 2 0 0 0 0 – 0 0 0 0 0 0 2 1 1 1 0 0 1 1 0
Mitochondria 3 13 1 7 6 140 231 210 – 336 296 364 1 0 0 17 6 5 50 36 103 99 114 80
Nucleus 0 0 0 1 0 2 2 3 – 4 3 0 1 2 2 0 4 1 0 0 0 1 0 3
Nuclear Periphery 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Nucleolus 0 0 0 0 1 0 1 1 – 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 3 8 17 – 15 17 18 2 0 0 2 0 0 5 2 4 2 12 17
Vac/Vac Membrane 5 0 4 1 17 4 13 5 – 10 4 11 1 0 0 3 6 7 12 11 12 18 35 51
Unique Cell Count 400 406 529 318 406 570 632 664 614 552 572 663 409 798 236 301 192 478 426 556 473 1004 1003
Labelled Cell Count 406 417 537 330 426 664 783 785 776 705 738 673 414 804 261 338 211 478 426 556 473 1004 1003


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.1 4.4 4.0 3.9 2.9 2.7 2.9 – 2.5 2.6 2.6 5.2 5.3 5.3 4.9 5.3 5.7 5.6 5.4 5.8
Std Deviation (1e-4) 0.6 0.5 0.9 1.2 1.4 1.1 1.0 0.9 – 0.8 1.2 0.7 1.0 1.2 0.9 1.6 1.4 1.8 1.8 1.4 1.8
Intensity Change (Log2) – – – -0.11 -0.18 -0.58 -0.7 -0.58 – -0.79 -0.73 -0.73 0.26 0.28 0.28 0.17 0.27 0.37 0.35 0.31 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5191 1.4064 1.1655 3.5936 1.8126 5.3484 3.809 3.9662 4.0635 4.2275 3.3121 4.4284 3.1236 4.0694 3.6263 3.9229 3.8397 3.675
Actin 0.0153 0.0007 0.0196 0.005 0 0.0009 0.0245 0.0002 0.0022 0.0065 0.0003 0.007 0.0002 0.001 0.0023 0.0002 0.0001 0.0003
Bud 0.0007 0.0014 0.0187 0.0007 0 0.003 0.0032 0.0013 0.0034 0.0007 0.0004 0.0012 0.0001 0.0012 0.0004 0.0001 0.0001 0.0001
Bud Neck 0.0064 0.0074 0.0002 0.0019 0 0.0005 0.0094 0.0002 0.0006 0.0172 0.0015 0.0008 0.0001 0.0005 0.0003 0.0002 0.0002 0.0005
Bud Periphery 0.0018 0.0009 0.0067 0.0007 0 0.0062 0.0046 0.0017 0.0084 0.0011 0.0002 0.0052 0.0001 0.0018 0.0006 0.0002 0.0001 0.0002
Bud Site 0.0241 0.011 0.014 0.0112 0 0.0006 0.0069 0.0049 0.0113 0.011 0.0007 0.0003 0.0003 0.0108 0.0025 0.0002 0.0002 0.0001
Cell Periphery 0.005 0.001 0.0004 0.0007 0 0.0007 0.0006 0.0005 0.0007 0.0007 0.0001 0.0002 0.0002 0.0005 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.0723 0.138 0.1701 0.0728 0.1454 0.2132 0.2352 0.3738 0.3335 0.3603 0.5415 0.3753 0.294 0.4559 0.3731 0.4463 0.292 0.4727
Cytoplasmic Foci 0.0942 0.061 0.0375 0.0574 0.1023 0.0487 0.0321 0.0119 0.0184 0.017 0.0503 0.0207 0.0203 0.0146 0.0137 0.012 0.0329 0.016
Eisosomes 0.0016 0.0003 0.0002 0.0002 0 0.0079 0.0003 0.0001 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0349 0.0071 0.016 0.0201 0.0013 0.0084 0.0232 0.0148 0.0152 0.0216 0.0209 0.0104 0.0204 0.0151 0.0163 0.0198 0.0039 0.0157
Endosome 0.112 0.0639 0.0579 0.2018 0.0044 0.075 0.0258 0.0101 0.0194 0.0211 0.0413 0.0164 0.0051 0.0162 0.0086 0.0128 0.0147 0.0076
Golgi 0.0098 0.0028 0.004 0.0284 0.0003 0.0053 0.0053 0.0004 0.0007 0.003 0.0022 0.0038 0.0003 0.0004 0.0009 0.0002 0.0006 0.0002
Lipid Particles 0.0858 0.0093 0.0161 0.0636 0.0151 0.0221 0.0081 0.001 0.0024 0.0018 0.0009 0.0039 0.0025 0.0011 0.0023 0.0003 0.0032 0.0005
Mitochondria 0.0652 0.0115 0.002 0.1471 0.0007 0.0304 0.0029 0.0068 0.0008 0.0039 0.001 0.0012 0.0003 0.0004 0.0007 0.0002 0.0003 0.0003
None 0.3341 0.5165 0.6207 0.3112 0.7227 0.5023 0.5469 0.5529 0.5592 0.5086 0.3052 0.5397 0.645 0.4624 0.5667 0.4948 0.6374 0.4754
Nuclear Periphery 0.015 0.0035 0.0018 0.0021 0.0004 0.0065 0.0138 0.0022 0.0045 0.0048 0.0032 0.002 0.0017 0.0034 0.002 0.003 0.0011 0.0016
Nucleolus 0.0017 0.0097 0.0008 0.0011 0.0003 0.0007 0.0043 0.0007 0.0005 0.0015 0.0002 0.0002 0.0003 0.0003 0.0004 0.0001 0.0002 0.0001
Nucleus 0.0414 0.0055 0.0018 0.0009 0.0005 0.0032 0.0218 0.004 0.0033 0.0054 0.0036 0.0026 0.0014 0.0042 0.002 0.0029 0.0019 0.004
Peroxisomes 0.0533 0.0021 0.0025 0.0292 0.0036 0.0065 0.0055 0.0009 0.0009 0.0034 0.0064 0.002 0.0026 0.0001 0.0011 0 0.0034 0.0001
Punctate Nuclear 0.0035 0.0137 0.0018 0.0024 0.002 0.0032 0.0155 0.0011 0.0038 0.0036 0.002 0.002 0.0022 0.0016 0.0022 0.0009 0.0038 0.0013
Vacuole 0.018 0.1212 0.0061 0.0386 0.0008 0.0483 0.0081 0.0101 0.0094 0.0055 0.0169 0.0045 0.0026 0.0079 0.0031 0.0051 0.0033 0.0027
Vacuole Periphery 0.0039 0.0117 0.0009 0.0032 0.0001 0.0063 0.0018 0.0005 0.0013 0.001 0.0012 0.0005 0.0002 0.0006 0.0003 0.0005 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.503 49.817 41.1251 50.1601 34.7718 55.6966 55.4069 53.5441 48.0245 53.9221
Translational Efficiency 0.6484 0.5452 0.6793 0.4645 0.6006 0.6824 0.6772 0.583 0.6487 0.5643

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
120 1121 248 1493 2419 1820 224 1677 2539 2941 472 3170

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.22 660.93 904.05 944.66 674.79 753.37 964.52 908.69 681.76 718.14 932.75 925.63
Standard Deviation 126.65 100.17 95.96 135.62 73.66 92.63 106.30 122.41 83.10 105.59 105.42 130.05
Intensity Change Log 2 — -0.315027 0.136878 0.200271 — 0.158920 0.515373 0.429350 — -0.081996 0.319851 0.308051

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001407 0.000227 0.000498 0.004291 0.000173 0.000688 0.000714 0.001320 0.000232 0.000512 0.000600 0.002719
Bud Neck 0.004081 0.017026 0.002472 0.011640 0.024275 0.034007 0.004819 0.012394 0.023320 0.027535 0.003586 0.012039
Bud Site 0.031592 0.006741 0.003396 0.035690 0.006972 0.014503 0.005386 0.026069 0.008135 0.011544 0.004340 0.030600
Cell Periphery 0.000465 0.000166 0.000414 0.001558 0.000320 0.000254 0.000722 0.000555 0.000327 0.000221 0.000560 0.001027
Cytoplasm 0.246965* 0.450332* 0.295235* 0.271291* 0.412441* 0.383698* 0.279024* 0.263434* 0.404621* 0.409096* 0.287541* 0.267134*
Cytoplasmic Foci 0.115112 0.232562* 0.045897 0.053618 0.221848* 0.268316* 0.051665 0.092013 0.216804* 0.254687* 0.048634 0.073930
Eisosomes 0.000198 0.000170 0.000145 0.000558 0.000479 0.000333 0.000218 0.000348 0.000465 0.000271 0.000180 0.000447
Endoplasmic Reticulum 0.004403 0.002974 0.011499 0.223792* 0.007090 0.002598 0.105205* 0.139402* 0.006963 0.002742 0.055969 0.179148*
Endosome 0.042080 0.023437 0.013762 0.016787 0.020071 0.031789 0.013556 0.040296 0.021111 0.028606 0.013664 0.029224
Golgi 0.033855 0.003006 0.000171 0.004136 0.006120 0.007222 0.000285 0.004374 0.007430 0.005615 0.000225 0.004262
Lipid Particles 0.006042 0.017959 0.010062 0.034512 0.033481 0.023763 0.020367 0.079764 0.032184 0.021551 0.014952 0.058451
Mitochondria 0.017552 0.005415 0.000415 0.001358 0.009165 0.013166 0.000290 0.002774 0.009561 0.010211 0.000355 0.002107
Mitotic Spindle 0.006370 0.001284 0.000877 0.011912 0.000809 0.005828 0.000446 0.013199 0.001072 0.004096 0.000672 0.012593
None 0.000695 0.007848 0.003124 0.001098 0.003260 0.002418 0.001236 0.003572 0.003139 0.004488 0.002228 0.002407
Nuclear Periphery 0.000704 0.001256 0.005315 0.006310 0.001160 0.000775 0.015667 0.012105 0.001139 0.000958 0.010227 0.009376
Nuclear Periphery Foci 0.002336 0.001598 0.013538 0.034038 0.000561 0.000515 0.064966 0.032919 0.000644 0.000927 0.037944 0.033446
Nucleolus 0.000467 0.003032 0.001424 0.000522 0.001755 0.001804 0.000417 0.001617 0.001695 0.002272 0.000946 0.001101
Nucleus 0.127396 0.130652* 0.436746* 0.074685 0.165217* 0.101486 0.196632* 0.067901 0.163429* 0.112603 0.322794* 0.071096
Peroxisomes 0.002598 0.006975 0.000787 0.001868 0.004980 0.011537 0.000679 0.003478 0.004868 0.009798 0.000736 0.002719
Vacuole 0.342859* 0.084913 0.153842 0.208315* 0.077892 0.092806 0.237513* 0.199853 0.090415 0.089797 0.193550 0.203838*
Vacuole Periphery 0.012824 0.002429 0.000383 0.002020 0.001930 0.002494 0.000195 0.002613 0.002445 0.002469 0.000294 0.002333

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.86 1.73 -3.55 -5.36 -4.80 -1.25 -8.44 -4.23 -1.27 -2.09 -1.12 -8.38 -6.48 -4.74 -5.24
Bud Neck -8.07 0.21 -7.88 2.94 -8.44 -4.37 13.91 7.60 10.16 -6.14 -2.38 15.53 8.47 10.50 -10.18
Bud Site 2.27 2.29 -0.68 -8.54 -7.39 -4.51 0.83 -6.93 -3.80 -7.33 -2.68 1.29 -9.75 -8.18 -10.16
Cell Periphery 3.83 0.02 -6.27 -8.66 -6.76 1.54 -5.96 -3.42 -4.14 1.89 3.24 -5.57 -7.96 -9.34 -5.03
Cytoplasm -8.39 -0.75 -0.94 16.78 -0.15 3.69 8.99 19.21 13.74 0.34 -0.38 12.23 21.33 21.02 0.17
Cytoplasmic Foci -11.11 5.44 4.53 28.74 -2.79 -7.36 29.69 27.21 30.98 -8.29 -7.24 36.38 34.23 42.91 -8.50
Eisosomes 0.74 1.10 -6.19 -6.78 -7.13 4.50 8.77 3.82 -0.07 -3.76 6.93 10.65 1.41 -4.80 -8.07
Endoplasmic Reticulum 1.05 -5.20 -24.50 -25.04 -23.48 6.61 -8.81 -21.02 -21.91 -3.81 6.62 -8.78 -31.93 -33.08 -16.38
Endosome 2.06 3.89 3.57 4.12 -1.42 -6.59 5.92 -10.62 -3.26 -14.77 -5.45 6.49 -5.85 0.27 -12.75
Golgi 2.40 2.71 2.35 -0.70 -5.89 -1.52 15.08 3.71 4.39 -9.42 2.05 11.85 4.91 3.53 -10.55
Lipid Particles -6.26 -2.59 -12.50 -6.53 -11.98 5.03 7.14 -12.12 -15.27 -16.29 6.22 10.98 -9.88 -15.98 -19.82
Mitochondria 1.09 1.78 1.68 5.60 -3.02 -2.70 10.22 7.04 8.54 -7.38 -0.68 10.03 8.08 10.12 -7.69
Mitotic Spindle 1.13 1.16 -1.98 -5.32 -5.35 -4.39 0.56 -6.55 -3.48 -6.81 -3.87 0.19 -8.29 -5.68 -8.56
None -5.40 -4.56 -1.34 5.05 3.49 1.18 3.46 -0.24 -1.06 -2.40 -1.82 2.25 1.46 2.79 -0.44
Nuclear Periphery -0.99 -7.94 -6.44 -6.00 -1.30 2.85 -5.95 -11.36 -11.81 0.75 1.26 -7.72 -12.68 -12.97 -0.13
Nuclear Periphery Foci 1.74 -6.37 -16.23 -17.04 -7.37 0.55 -12.02 -16.88 -16.91 5.04 -2.89 -12.96 -24.30 -24.05 0.79
Nucleolus -4.26 -5.46 -1.36 3.58 2.29 -0.60 4.97 0.26 0.82 -4.90 -1.91 2.89 1.54 3.35 -1.57
Nucleus -0.11 -11.05 2.59 9.71 18.99 14.50 -2.92 23.10 7.85 9.84 12.53 -12.09 24.14 11.94 19.58
Peroxisomes -6.03 3.31 0.84 7.17 -4.90 -7.50 14.99 3.60 9.09 -8.08 -7.79 15.07 6.63 11.70 -9.36
Vacuole 10.27 5.83 4.69 -21.34 -3.16 -4.07 -12.16 -23.12 -19.46 3.27 0.34 -13.32 -27.19 -28.85 0.30
Vacuole Periphery 1.56 1.99 1.70 1.14 -2.69 -2.76 14.85 -1.24 0.13 -5.79 -0.50 8.09 0.54 1.03 -5.86
Standard name
Human Ortholog
Description Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
Localization
Cell Percentages cytoplasm (28%), ER (5%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ipt1

Ipt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ipt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available