Standard name | |
---|---|
Human Ortholog | |
Description | Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 1 | 16 | 23 | 33 | 9 | 13 | 11 | 10 | 28 | 1 | 2 | 0 | 1 | 1 | 3 | 3 | 0 | 2 | 8 | 8 | 11 |
Bud | 1 | 0 | 1 | 2 | 4 | 4 | 30 | 21 | 13 | 21 | 16 | 20 | 0 | 0 | 0 | 1 | 0 | 3 | 8 | 5 | 0 | 8 | 51 | 78 |
Bud Neck | 4 | 0 | 7 | 2 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 5 | 8 |
Bud Site | 1 | 0 | 0 | 0 | 2 | 1 | 5 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 5 | 27 | 25 | 28 | 22 | 11 | 18 | 24 | 23 | 1 | 3 | 6 | 14 | 10 | 10 | 0 | 0 | 0 | 2 | 4 | 3 |
Cytoplasm | 148 | 202 | 138 | 127 | 86 | 94 | 156 | 137 | 139 | 192 | 125 | 121 | 241 | 401 | 406 | 116 | 83 | 117 | 105 | 122 | 72 | 184 | 160 | 123 |
Endoplasmic Reticulum | 5 | 4 | 2 | 6 | 94 | 90 | 95 | 38 | 28 | 26 | 14 | 23 | 7 | 4 | 8 | 10 | 9 | 11 | 12 | 10 | 5 | 12 | 59 | 85 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 3 | 9 | 4 | 1 | 3 | 3 | 2 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 6 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 9 | 2 | 1 | 2 | 4 | 8 | 22 | 6 | 24 | 8 | 21 | 8 | 1 | 1 | 1 | 2 | 1 | 3 | 33 | 10 | 17 | 22 | 27 | 12 |
Nucleus | 0 | 1 | 6 | 0 | 1 | 3 | 5 | 2 | 4 | 2 | 6 | 2 | 1 | 1 | 0 | 34 | 15 | 29 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 4 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 4 | 0 | 0 | 0 | 6 | 5 | 9 | 2 | 2 | 1 | 1 | 6 | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 13 | 8 |
Vac/Vac Membrane | 0 | 29 | 13 | 7 | 32 | 19 | 22 | 21 | 18 | 31 | 14 | 26 | 2 | 3 | 0 | 125 | 88 | 104 | 13 | 71 | 49 | 18 | 18 | 11 |
Unique Cell Count | 164 | 226 | 154 | 141 | 229 | 224 | 337 | 226 | 215 | 270 | 184 | 203 | 254 | 414 | 421 | 255 | 180 | 239 | 189 | 234 | 165 | 276 | 368 | 357 |
Labelled Cell Count | 173 | 240 | 170 | 152 | 289 | 272 | 408 | 266 | 259 | 316 | 238 | 267 | 258 | 418 | 430 | 314 | 218 | 286 | 189 | 234 | 165 | 276 | 368 | 357 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.1 | 5.3 | 5.9 | 15.7 | 15.9 | 13.5 | 12.9 | 11.4 | 12.4 | 11.9 | 15.1 | 4.8 | 5.0 | 5.4 | 31.6 | 35.3 | 40.7 | 5.2 | 6.5 | 8.1 |
Std Deviation (1e-4) | 8.6 | 3.1 | 5.0 | 4.6 | 11.1 | 11.5 | 9.1 | 8.2 | 9.3 | 8.1 | 8.8 | 10.4 | 2.5 | 2.3 | 2.8 | 28.2 | 33.3 | 36.5 | 4.4 | 5.1 | 6.2 |
Intensity Change (Log2) | – | – | – | 0.14 | 1.56 | 1.58 | 1.35 | 1.28 | 1.11 | 1.22 | 1.17 | 1.51 | -0.15 | -0.09 | 0.02 | 2.57 | 2.73 | 2.94 | -0.02 | 0.29 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 3.0 | 3.8 | 3.7 | 0 | 2.7 | 0 | 2.5 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.5 | 3.5 | 2.7 | 3.2 | 3.4 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 4.4 | 4.3 | 3.7 | 4.0 | 0 | 3.3 | 4.6 | 4.3 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Cytoplasm | 0.1 | -10.1 | -9.3 | -9.1 | -6.2 | -5.5 | -4.4 | -4.8 | -6.3 | 2.0 | 3.5 | 3.2 | -8.9 | -8.4 | -8.2 |
Endoplasmic Reticulum | 0 | 8.8 | 8.7 | 6.9 | 4.8 | 4.1 | 3.3 | 2.7 | 3.7 | 0 | 0 | 0 | 0 | 1.9 | 1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.9 | 0 | 4.0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | 0 | 0 | 0 | 0 | 3.1 | 1.7 | 2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.2 | 1.6 | 0 | -0.8 | 0.3 | 0 | 1.0 | -0.3 | 1.3 | -4.0 | 0 | 0 | 8.4 | 8.0 | 7.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.5463 | 26.491 | 15.788 | 18.8014 | 13.1731 | 23.4923 | 21.7997 | 22.3313 | 19.8623 | 29.1473 | 15.4094 | 18.9942 | 17.1607 | 19.3297 | 11.96 | 12.2207 | 13.904 | 16.0245 |
Actin | 0.0071 | 0.0109 | 0.0121 | 0.0048 | 0.0132 | 0.0092 | 0.0068 | 0.0182 | 0.0093 | 0.0206 | 0.0145 | 0.0116 | 0.0121 | 0.012 | 0.0179 | 0.0452 | 0.0093 | 0.0136 |
Bud | 0.0168 | 0.0351 | 0.0334 | 0.0372 | 0.0473 | 0.0103 | 0.017 | 0.0257 | 0.0235 | 0.0219 | 0.0301 | 0.0192 | 0.0166 | 0.0321 | 0.0371 | 0.0542 | 0.0176 | 0.0123 |
Bud Neck | 0.0022 | 0.0023 | 0.01 | 0.001 | 0.0044 | 0.012 | 0.0019 | 0.0017 | 0.0026 | 0.0028 | 0.0034 | 0.0122 | 0.002 | 0.0023 | 0.0083 | 0.008 | 0.0023 | 0.0143 |
Bud Periphery | 0.049 | 0.0533 | 0.072 | 0.1157 | 0.1074 | 0.0401 | 0.0675 | 0.0606 | 0.0874 | 0.077 | 0.0964 | 0.0761 | 0.0501 | 0.0531 | 0.0993 | 0.0945 | 0.0826 | 0.0604 |
Bud Site | 0.0226 | 0.0703 | 0.0326 | 0.0047 | 0.0084 | 0.0015 | 0.0423 | 0.0773 | 0.0489 | 0.0077 | 0.0347 | 0.0048 | 0.0205 | 0.1018 | 0.0456 | 0.0244 | 0.0112 | 0.0025 |
Cell Periphery | 0.0711 | 0.1114 | 0.0925 | 0.0555 | 0.0677 | 0.0474 | 0.0954 | 0.0862 | 0.0986 | 0.0661 | 0.0666 | 0.0683 | 0.0605 | 0.0809 | 0.0579 | 0.0464 | 0.0749 | 0.0281 |
Cytoplasm | 0.1103 | 0.0836 | 0.1101 | 0.0929 | 0.0617 | 0.1033 | 0.0827 | 0.082 | 0.0733 | 0.049 | 0.0934 | 0.1368 | 0.1174 | 0.1604 | 0.087 | 0.0315 | 0.0854 | 0.148 |
Cytoplasmic Foci | 0.0075 | 0.0016 | 0.0068 | 0.0018 | 0.0018 | 0.004 | 0.0039 | 0.0015 | 0.0028 | 0.0095 | 0.0012 | 0.0039 | 0.0097 | 0.0037 | 0.0063 | 0.0203 | 0.0029 | 0.0047 |
Eisosomes | 0.0018 | 0.0014 | 0.0008 | 0.0016 | 0.001 | 0.0031 | 0.002 | 0.0109 | 0.0011 | 0.0008 | 0.0006 | 0.0012 | 0.001 | 0.0012 | 0.0012 | 0.0012 | 0.0008 | 0.002 |
Endoplasmic Reticulum | 0.5426 | 0.4936 | 0.4102 | 0.4767 | 0.4901 | 0.6667 | 0.5121 | 0.5199 | 0.4319 | 0.5332 | 0.5128 | 0.5134 | 0.4797 | 0.4361 | 0.3792 | 0.3657 | 0.56 | 0.5901 |
Endosome | 0.0048 | 0.002 | 0.0047 | 0.0025 | 0.0044 | 0.0036 | 0.0032 | 0.0036 | 0.0029 | 0.0306 | 0.0028 | 0.0029 | 0.0065 | 0.0039 | 0.0054 | 0.0019 | 0.0076 | 0.0061 |
Golgi | 0.0039 | 0.0013 | 0.003 | 0.0008 | 0.0021 | 0.004 | 0.0013 | 0.0025 | 0.0014 | 0.0029 | 0.0009 | 0.0018 | 0.0019 | 0.0024 | 0.0025 | 0.0037 | 0.002 | 0.0025 |
Lipid Particles | 0.0115 | 0.0167 | 0.019 | 0.0023 | 0.0121 | 0.0131 | 0.0121 | 0.0163 | 0.018 | 0.0269 | 0.0081 | 0.013 | 0.009 | 0.0184 | 0.0124 | 0.0058 | 0.0133 | 0.0077 |
Mitochondria | 0.0057 | 0.0009 | 0.0025 | 0.011 | 0.001 | 0.0013 | 0.0026 | 0.0015 | 0.0044 | 0.0018 | 0.0016 | 0.0012 | 0.0039 | 0.0013 | 0.0033 | 0.0018 | 0.0027 | 0.0018 |
None | 0.0511 | 0.0052 | 0.0784 | 0.0831 | 0.0564 | 0.0252 | 0.0581 | 0.0083 | 0.0789 | 0.0708 | 0.028 | 0.0634 | 0.1269 | 0.0318 | 0.1589 | 0.1487 | 0.0721 | 0.0566 |
Nuclear Periphery | 0.0353 | 0.0447 | 0.0335 | 0.0247 | 0.0362 | 0.0207 | 0.0293 | 0.0292 | 0.0339 | 0.0266 | 0.0293 | 0.0201 | 0.0307 | 0.0197 | 0.0281 | 0.0275 | 0.0097 | 0.0201 |
Nucleolus | 0.0018 | 0.0019 | 0.0033 | 0.011 | 0.0022 | 0.0008 | 0.0024 | 0.0021 | 0.0031 | 0.0007 | 0.0008 | 0.0016 | 0.0021 | 0.0016 | 0.0021 | 0.0083 | 0.0034 | 0.0005 |
Nucleus | 0.0077 | 0.0235 | 0.0235 | 0.0439 | 0.0194 | 0.0072 | 0.0085 | 0.0166 | 0.022 | 0.0056 | 0.0144 | 0.0116 | 0.0061 | 0.0107 | 0.0106 | 0.0335 | 0.0132 | 0.0048 |
Peroxisomes | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.003 | 0.0065 | 0.0097 | 0.0017 | 0.01 | 0.0037 | 0.0047 | 0.007 | 0.0118 | 0.0041 | 0.0072 | 0.0043 | 0.0014 | 0.0015 | 0.0065 | 0.0652 | 0.0032 | 0.001 |
Vacuole | 0.0402 | 0.0318 | 0.0383 | 0.0227 | 0.0485 | 0.0206 | 0.0427 | 0.027 | 0.0408 | 0.0378 | 0.0511 | 0.0302 | 0.0378 | 0.0228 | 0.026 | 0.0101 | 0.024 | 0.0194 |
Vacuole Periphery | 0.0036 | 0.0019 | 0.0032 | 0.0042 | 0.0045 | 0.0023 | 0.0036 | 0.0014 | 0.0033 | 0.0027 | 0.0019 | 0.0021 | 0.0037 | 0.0022 | 0.0039 | 0.0017 | 0.0016 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 699.7993 | 400.5638 | 996.0631 | 1746.5895 | 937.5644 | 751.4611 | 711.7088 | 1015.3156 | 1099.8774 | 1086.7268 |
Translational Efficiency | 2.6604 | 2.7515 | 2.9619 | 1.2542 | 2.3393 | 3.7424 | 2.023 | 2.0914 | 2.8703 | 2.4898 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
170 | 649 | 171 | 739 | 461 | 1296 | 187 | 1354 | 631 | 1945 | 358 | 2093 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 691.90 | 705.40 | 6537.27 | 9921.83 | 658.37 | 710.62 | 7076.18 | 8054.86 | 667.40 | 708.88 | 6818.77 | 8714.05 |
Standard Deviation | 92.02 | 234.31 | 1791.09 | 2998.33 | 114.77 | 152.69 | 2290.86 | 2945.16 | 110.12 | 184.01 | 2084.72 | 3095.43 |
Intensity Change Log 2 | — | 0.027878 | 3.240053 | 3.841971 | — | 0.110180 | 3.426000 | 3.612889 | — | 0.068594 | 3.333713 | 3.734808 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000072 | 0.001683 | 0.000289 | 0.001975 | 0.000397 | 0.000782 | 0.000327 | 0.001335 | 0.000310 | 0.001083 | 0.000309 | 0.001561 |
Bud Neck | 0.023226 | 0.037383 | 0.003200 | 0.005345 | 0.016363 | 0.047223 | 0.011781 | 0.002189 | 0.018212 | 0.043940 | 0.007682 | 0.003303 |
Bud Site | 0.003600 | 0.043032 | 0.001873 | 0.008490 | 0.004492 | 0.038025 | 0.002619 | 0.007675 | 0.004251 | 0.039696 | 0.002262 | 0.007963 |
Cell Periphery | 0.000401 | 0.004240 | 0.138113* | 0.113409* | 0.000415 | 0.001930 | 0.124384* | 0.113691* | 0.000411 | 0.002701 | 0.130942* | 0.113591* |
Cytoplasm | 0.367282* | 0.292792* | 0.000065 | 0.005942 | 0.453531* | 0.357541* | 0.000505 | 0.009883 | 0.430294* | 0.335936* | 0.000295 | 0.008491 |
Cytoplasmic Foci | 0.297610* | 0.241752* | 0.000028 | 0.001761 | 0.267426* | 0.213133* | 0.000646 | 0.001593 | 0.275558* | 0.222683* | 0.000351 | 0.001652 |
Eisosomes | 0.000450 | 0.000333 | 0.006751 | 0.001561 | 0.000325 | 0.000239 | 0.007298 | 0.000734 | 0.000359 | 0.000270 | 0.007037 | 0.001026 |
Endoplasmic Reticulum | 0.012473 | 0.019760 | 0.628803* | 0.498935* | 0.007448 | 0.019310 | 0.491889* | 0.619486* | 0.008802 | 0.019460 | 0.557286* | 0.576921* |
Endosome | 0.024900 | 0.055174 | 0.004019 | 0.008583 | 0.013177 | 0.035850 | 0.009690 | 0.008434 | 0.016335 | 0.042298 | 0.006981 | 0.008487 |
Golgi | 0.001970 | 0.006629 | 0.021151 | 0.020080 | 0.003512 | 0.008228 | 0.016225 | 0.016015 | 0.003096 | 0.007695 | 0.018578 | 0.017451 |
Lipid Particles | 0.020666 | 0.013382 | 0.012426 | 0.158002* | 0.014611 | 0.007272 | 0.100461* | 0.108376* | 0.016243 | 0.009311 | 0.058411 | 0.125898* |
Mitochondria | 0.014195 | 0.013779 | 0.000297 | 0.000922 | 0.008041 | 0.015231 | 0.000249 | 0.001653 | 0.009699 | 0.014747 | 0.000272 | 0.001395 |
Mitotic Spindle | 0.000044 | 0.001857 | 0.000200 | 0.001754 | 0.000281 | 0.003785 | 0.000377 | 0.000423 | 0.000217 | 0.003142 | 0.000293 | 0.000893 |
None | 0.000728 | 0.004699 | 0.000031 | 0.003191 | 0.002877 | 0.002632 | 0.000083 | 0.002427 | 0.002298 | 0.003322 | 0.000058 | 0.002697 |
Nuclear Periphery | 0.000486 | 0.000743 | 0.034187 | 0.015401 | 0.000536 | 0.000506 | 0.032492 | 0.009546 | 0.000522 | 0.000585 | 0.033302 | 0.011613 |
Nuclear Periphery Foci | 0.000186 | 0.000695 | 0.003137 | 0.012888 | 0.002592 | 0.000531 | 0.015503 | 0.005774 | 0.001944 | 0.000586 | 0.009596 | 0.008286 |
Nucleolus | 0.000702 | 0.002306 | 0.000036 | 0.000061 | 0.001564 | 0.001270 | 0.000027 | 0.000079 | 0.001331 | 0.001616 | 0.000032 | 0.000073 |
Nucleus | 0.153116* | 0.051860 | 0.000156 | 0.001173 | 0.147326* | 0.077341 | 0.000622 | 0.001163 | 0.148886* | 0.068839 | 0.000400 | 0.001166 |
Peroxisomes | 0.007777 | 0.027089 | 0.000006 | 0.000202 | 0.006938 | 0.014722 | 0.000006 | 0.000648 | 0.007164 | 0.018849 | 0.000006 | 0.000491 |
Vacuole | 0.068273 | 0.174242 | 0.135775 | 0.133242 | 0.046564 | 0.150805 | 0.179010 | 0.082456 | 0.052413 | 0.158626 | 0.158358 | 0.100387 |
Vacuole Periphery | 0.001844 | 0.006569 | 0.009457 | 0.007083 | 0.001586 | 0.003642 | 0.005807 | 0.006421 | 0.001655 | 0.004619 | 0.007550 | 0.006655 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.60 | -1.84 | -2.53 | -0.67 | -2.25 | -1.43 | 0.07 | -2.67 | -1.94 | -2.80 | -2.77 | -0.19 | -3.57 | -1.76 | -3.58 |
Bud Neck | -2.64 | 4.64 | 3.88 | 8.96 | -2.91 | -7.19 | 0.65 | 4.24 | 15.56 | 4.86 | -7.54 | 3.21 | 5.37 | 17.88 | 3.74 |
Bud Site | -8.10 | 0.93 | -2.97 | 6.43 | -3.40 | -9.68 | 0.46 | -2.53 | 8.20 | -2.92 | -12.46 | 1.01 | -3.53 | 10.42 | -4.51 |
Cell Periphery | -2.82 | -12.82 | -22.06 | -20.67 | 2.08 | -2.74 | -11.80 | -27.65* | -27.06* | 0.81 | -3.91 | -17.38 | -35.28 | -34.07* | 2.02 |
Cytoplasm | 3.33 | 19.63 | 19.20 | 27.29 | -5.19 | 6.77 | 38.83 | 37.71 | 44.85 | -6.49 | 7.84 | 42.96 | 41.84 | 52.17 | -8.27 |
Cytoplasmic Foci | 3.07 | 19.50 | 19.36 | 27.90 | -3.59 | 4.83 | 27.92 | 27.93 | 36.73 | -0.66 | 5.51 | 34.00 | 33.86 | 46.04 | -1.97 |
Eisosomes | 0.90 | -4.79 | -2.24 | -2.51 | 3.74 | 1.80 | -4.00 | -3.60 | -4.55 | 3.74 | 1.71 | -6.07 | -3.60 | -4.12 | 5.38 |
Endoplasmic Reticulum | -0.74 | -25.19 | -29.54 | -32.35* | 5.29 | -2.49 | -17.93 | -60.11* | -59.47* | -4.69 | -2.45 | -29.71 | -65.02 | -65.90* | -0.84 |
Endosome | -5.06 | 4.44 | 3.42 | 12.65 | -7.79 | -9.43 | 1.61 | 3.13 | 14.16 | 1.60 | -10.66 | 5.10 | 4.59 | 18.86 | -1.88 |
Golgi | -5.36 | -9.65 | -9.69 | -6.75 | 0.50 | -3.09 | -6.62 | -8.73 | -5.74 | 0.17 | -4.15 | -10.39 | -11.98 | -8.60 | 0.84 |
Lipid Particles | 1.90 | 1.80 | -15.63 | -17.50 | -17.11 | 3.91 | -5.81 | -17.80 | -20.32 | -0.39 | 3.99 | -5.14 | -23.77 | -26.67 | -7.15 |
Mitochondria | 0.05 | 1.92 | 1.79 | 6.15 | -2.33 | -2.28 | 2.89 | 2.28 | 7.65 | -4.26 | -1.67 | 3.40 | 2.91 | 9.72 | -4.86 |
Mitotic Spindle | -3.75 | -5.66 | -1.56 | 0.08 | -1.42 | -3.70 | -1.05 | -1.27 | 3.57 | 0.08 | -4.51 | -1.07 | -1.70 | 2.95 | -1.47 |
None | -3.10 | 6.31 | -1.82 | 0.78 | -2.32 | 0.54 | 5.68 | 0.57 | 0.05 | -4.14 | -1.36 | 6.19 | -0.57 | 0.60 | -4.40 |
Nuclear Periphery | -0.88 | -12.93 | -17.70 | -16.71 | 6.90 | 0.21 | -10.27 | -21.59 | -20.96 | 7.38 | -0.39 | -15.99 | -27.27 | -26.22 | 10.47 |
Nuclear Periphery Foci | -3.96 | -7.56 | -8.01 | -7.67 | -6.05 | 1.34 | -4.30 | -1.78 | -9.25 | 3.80 | 1.21 | -4.23 | -4.66 | -11.36 | 1.08 |
Nucleolus | -4.28 | 6.41 | 5.95 | 6.00 | -1.58 | 0.86 | 3.83 | 3.68 | 7.09 | -2.34 | -0.71 | 4.38 | 4.20 | 9.14 | -2.81 |
Nucleus | 7.36 | 11.78 | 11.60 | 12.99 | -3.16 | 9.01 | 20.88 | 20.82 | 22.03 | -1.19 | 11.85 | 23.96 | 23.75 | 25.44 | -3.31 |
Peroxisomes | -5.86 | 6.35 | 6.18 | 8.75 | -2.68 | -4.68 | 5.84 | 5.28 | 11.75 | -4.85 | -7.36 | 7.73 | 7.18 | 14.07 | -5.41 |
Vacuole | -6.86 | -4.11 | -5.21 | 2.98 | -0.12 | -14.54 | -9.60 | -6.99 | 9.63 | 6.94 | -15.86 | -10.80 | -9.38 | 9.09 | 5.93 |
Vacuole Periphery | -3.79 | -5.49 | -7.79 | -0.63 | 1.63 | -3.43 | -4.40 | -6.69 | -4.22 | -0.41 | -5.03 | -6.91 | -9.29 | -3.56 | 1.09 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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