Standard name | |
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Description | Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 1 | 4 | 8 | 7 | 1 | 1 | 5 | 8 |
Bud | 7 | 1 | 23 | 15 | 6 | 13 | – | 32 | 19 | 11 | 9 | 17 | 1 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 4 | 0 | 0 | 3 | – | 10 | 9 | 10 | 5 | 15 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 11 | 9 | 3 | 4 | 2 | 2 | – | 4 | 6 | 2 | 2 | 3 | 4 | 5 | 5 | 2 | 3 | 7 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 29 | 31 | 26 | 16 | 23 | 15 | – | 30 | 14 | 12 | 10 | 9 | 7 | 9 | 13 | 5 | 10 | 15 | 6 | 4 | 2 | 0 | 1 | 2 |
Endoplasmic Reticulum | 11 | 5 | 4 | 2 | 1 | 2 | – | 2 | 2 | 6 | 1 | 0 | 8 | 9 | 3 | 3 | 4 | 2 | 1 | 3 | 1 | 1 | 0 | 1 |
Endosome | 16 | 18 | 1 | 1 | 39 | 4 | – | 8 | 1 | 1 | 0 | 0 | 7 | 9 | 20 | 4 | 4 | 3 | 32 | 18 | 18 | 6 | 15 | 17 |
Golgi | 6 | 15 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 38 | 30 | 15 | 13 | 6 | 19 | 11 | 11 | 5 | 5 | 25 | 16 |
Mitochondria | 329 | 146 | 249 | 252 | 200 | 383 | – | 834 | 518 | 489 | 352 | 445 | 141 | 134 | 78 | 4 | 6 | 12 | 337 | 215 | 208 | 77 | 143 | 148 |
Nucleus | 4 | 3 | 2 | 1 | 4 | 12 | – | 22 | 7 | 3 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 3 | 0 | 0 | 2 | 12 | – | 15 | 21 | 25 | 12 | 17 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 0 |
Nucleolus | 2 | 1 | 0 | 1 | 4 | 9 | – | 45 | 24 | 31 | 15 | 20 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Peroxisomes | 1 | 5 | 1 | 4 | 17 | 2 | – | 24 | 0 | 3 | 0 | 2 | 1 | 0 | 10 | 1 | 0 | 2 | 8 | 2 | 8 | 4 | 11 | 7 |
SpindlePole | 3 | 4 | 7 | 2 | 8 | 8 | – | 27 | 10 | 8 | 4 | 14 | 1 | 5 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 65 | 65 | 14 | 5 | 32 | 33 | – | 57 | 33 | 16 | 16 | 15 | 28 | 27 | 15 | 7 | 8 | 20 | 30 | 21 | 11 | 2 | 10 | 31 |
Unique Cell Count | 404 | 230 | 272 | 271 | 257 | 409 | 924 | 549 | 521 | 366 | 463 | 183 | 181 | 128 | 37 | 35 | 72 | 462 | 299 | 270 | 105 | 226 | 246 | |
Labelled Cell Count | 492 | 308 | 336 | 305 | 338 | 498 | 1110 | 664 | 617 | 433 | 569 | 245 | 233 | 160 | 43 | 44 | 89 | 462 | 299 | 270 | 105 | 226 | 246 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.4 | 4.1 | 4.5 | 5.3 | 4.4 | – | 4.5 | 4.0 | 4.2 | 4.2 | 4.0 | 6.5 | 6.5 | 6.2 | 7.8 | 8.4 | 7.9 | 6.0 | 6.5 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.2 | 2.0 | 1.7 | 1.6 | – | 1.4 | 1.2 | 1.0 | 1.2 | 1.0 | 1.2 | 1.2 | 1.0 | 1.4 | 2.0 | 1.7 | 1.5 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.39 | 0.11 | – | 0.13 | -0.01 | 0.05 | 0.04 | -0.02 | 0.69 | 0.68 | 0.61 | 0.94 | 1.05 | 0.96 | 0.57 | 0.68 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -3.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.7 | -3.7 | -3.0 | 0 | 0 | -1.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -0.2 | -3.2 | – | -4.3 | -4.4 | -4.5 | -3.7 | -4.7 | -2.3 | -1.8 | 0.2 | 0 | 0 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 |
Endosome | 0 | 6.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 7.9 | 6.9 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | -4.3 | -5.1 | -7.4 | -12.0 | -11.0 | -13.2 |
Nucleus | 0 | 0 | 2.0 | – | 1.7 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 4.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | 3.0 | 1.5 | – | 0.6 | 0.5 | -1.5 | -0.5 | -1.3 | 3.7 | 3.6 | 2.4 | 0 | 0 | 5.7 |