Standard name
Description Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Bud 0 0 0.08 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0.07 0.13 0.1 0.06 0.09 0 – 0 0 0 0 0 0 0.05 0.1 0.14 0.29 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.08 0.11 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0.15 0 – 0 0 0 0 0 0 0.05 0.16 0.11 0.11 0 0.07 0.06 0.07 0.06 0.07 0.07
Golgi 0 0.07 0 0 0 0 – 0 0 0 0 0 0.21 0.17 0.12 0.35 0.17 0.26 0 0 0 0.05 0.12 0.07
Mitochondria 0.81 0.63 0.92 0.93 0.78 0.94 – 0.9 0.94 0.94 0.96 0.96 0.77 0.74 0.61 0.11 0.17 0.17 0.73 0.72 0.77 0.74 0.64 0.6
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.28 0.05 0 0.12 0.08 – 0.06 0.06 0 0 0 0.15 0.15 0.12 0.19 0.23 0.28 0.07 0.07 0 0 0.05 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 2 1 0 0 0 – 0 0 0 0 0 3 3 0 2 1 4 8 7 1 1 5 8
Bud 7 1 23 15 6 13 – 32 19 11 9 17 1 1 1 1 0 2 3 2 0 0 0 3
Bud Neck 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 4 2 2 0 2 2
Bud Site 0 0 4 0 0 3 – 10 9 10 5 15 0 0 0 0 0 1 – – – – – –
Cell Periphery 11 9 3 4 2 2 – 4 6 2 2 3 4 5 5 2 3 7 0 0 1 0 1 0
Cytoplasm 29 31 26 16 23 15 – 30 14 12 10 9 7 9 13 5 10 15 6 4 2 0 1 2
Endoplasmic Reticulum 11 5 4 2 1 2 – 2 2 6 1 0 8 9 3 3 4 2 1 3 1 1 0 1
Endosome 16 18 1 1 39 4 – 8 1 1 0 0 7 9 20 4 4 3 32 18 18 6 15 17
Golgi 6 15 0 2 0 0 – 0 0 0 0 0 38 30 15 13 6 19 11 11 5 5 25 16
Mitochondria 329 146 249 252 200 383 – 834 518 489 352 445 141 134 78 4 6 12 337 215 208 77 143 148
Nucleus 4 3 2 1 4 12 – 22 7 3 7 12 0 0 0 0 0 1 1 1 0 0 1 1
Nuclear Periphery 1 3 0 0 2 12 – 15 21 25 12 17 4 0 0 0 0 0 4 1 1 0 1 0
Nucleolus 2 1 0 1 4 9 – 45 24 31 15 20 1 1 0 1 1 0 1 0 1 1 1 1
Peroxisomes 1 5 1 4 17 2 – 24 0 3 0 2 1 0 10 1 0 2 8 2 8 4 11 7
SpindlePole 3 4 7 2 8 8 – 27 10 8 4 14 1 5 0 0 1 1 3 2 1 1 1 0
Vac/Vac Membrane 65 65 14 5 32 33 – 57 33 16 16 15 28 27 15 7 8 20 30 21 11 2 10 31
Unique Cell Count 404 230 272 271 257 409 924 549 521 366 463 183 181 128 37 35 72 462 299 270 105 226 246
Labelled Cell Count 492 308 336 305 338 498 1110 664 617 433 569 245 233 160 43 44 89 462 299 270 105 226 246


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.4 4.1 4.5 5.3 4.4 – 4.5 4.0 4.2 4.2 4.0 6.5 6.5 6.2 7.8 8.4 7.9 6.0 6.5 6.9
Std Deviation (1e-4) 0.8 1.4 1.2 2.0 1.7 1.6 – 1.4 1.2 1.0 1.2 1.0 1.2 1.2 1.0 1.4 2.0 1.7 1.5 1.2 1.6
Intensity Change (Log2) – – – 0.14 0.39 0.11 – 0.13 -0.01 0.05 0.04 -0.02 0.69 0.68 0.61 0.94 1.05 0.96 0.57 0.68 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -3.1 0 – 0 0 0 0 0 -3.7 -3.7 -3.0 0 0 -1.7
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -0.2 -3.2 – -4.3 -4.4 -4.5 -3.7 -4.7 -2.3 -1.8 0.2 0 0 2.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 2.2 0 0 0 0
Endosome 0 6.4 0 – 0 0 0 0 0 0 0 6.4 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 7.9 6.9 0 0 0 0
Mitochondria 0 -4.4 0 – 0 0 0 0 0 -4.3 -5.1 -7.4 -12.0 -11.0 -13.2
Nucleus 0 0 2.0 – 1.7 0 0 0 1.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 4.0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.4 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 3.0 1.5 – 0.6 0.5 -1.5 -0.5 -1.3 3.7 3.6 2.4 0 0 5.7