Standard name | |
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Human Ortholog | |
Description | Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 4 | 0 | 2 | 8 | 17 | 16 | 39 | 35 | 34 | 36 | 36 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | 9 | 18 | 21 | 38 | 59 | 98 | 119 | 71 | 111 | 104 | 120 | 5 | 10 | 20 | 36 | 20 | 2 | 1 | 2 | 2 | 1 | 1 | 3 |
Endoplasmic Reticulum | 1 | 3 | 2 | 4 | 2 | 4 | 3 | 1 | 1 | 3 | 2 | 0 | 2 | 5 | 6 | 8 | 7 | 1 | 0 | 1 | 2 | 1 | 0 | 5 |
Endosome | 132 | 4 | 127 | 94 | 213 | 97 | 198 | 134 | 63 | 48 | 38 | 93 | 11 | 13 | 57 | 40 | 44 | 12 | 130 | 71 | 147 | 84 | 107 | 143 |
Golgi | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 3 | 2 | 0 | 1 |
Mitochondria | 37 | 100 | 68 | 73 | 86 | 229 | 169 | 285 | 261 | 337 | 310 | 365 | 1 | 0 | 0 | 10 | 18 | 1 | 15 | 4 | 6 | 5 | 9 | 7 |
Nucleus | 10 | 12 | 3 | 0 | 1 | 3 | 7 | 4 | 1 | 1 | 1 | 3 | 5 | 0 | 8 | 3 | 8 | 0 | 2 | 2 | 0 | 3 | 1 | 1 |
Nuclear Periphery | 4 | 5 | 13 | 5 | 0 | 5 | 7 | 1 | 2 | 2 | 3 | 1 | 3 | 0 | 1 | 3 | 4 | 0 | 28 | 6 | 33 | 8 | 9 | 11 |
Nucleolus | 21 | 7 | 24 | 10 | 10 | 34 | 32 | 37 | 31 | 21 | 31 | 47 | 4 | 3 | 7 | 1 | 9 | 4 | 2 | 0 | 0 | 3 | 0 | 0 |
Peroxisomes | 2 | 0 | 1 | 1 | 10 | 17 | 31 | 39 | 24 | 36 | 17 | 36 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 16 | 4 | 7 | 9 | 36 | 58 | 63 | 91 | 73 | 91 | 78 | 121 | 4 | 5 | 21 | 5 | 12 | 4 | 4 | 1 | 3 | 3 | 4 | 3 |
Vac/Vac Membrane | 256 | 114 | 168 | 61 | 108 | 74 | 94 | 59 | 39 | 48 | 40 | 47 | 32 | 45 | 115 | 66 | 73 | 13 | 199 | 108 | 119 | 70 | 84 | 76 |
Unique Cell Count | 362 | 184 | 305 | 202 | 372 | 418 | 516 | 566 | 428 | 515 | 457 | 621 | 50 | 64 | 190 | 126 | 136 | 25 | 394 | 206 | 323 | 185 | 223 | 265 |
Labelled Cell Count | 504 | 263 | 432 | 286 | 512 | 597 | 719 | 809 | 601 | 738 | 661 | 874 | 67 | 82 | 239 | 175 | 197 | 38 | 394 | 206 | 323 | 185 | 223 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 4.9 | 6.1 | 6.1 | 5.6 | 4.8 | 5.0 | 4.7 | 4.3 | 4.0 | 4.0 | 4.3 | 6.1 | 6.2 | 6.5 | 5.5 | 6.4 | 7.6 | 7.4 | 7.4 | 7.6 |
Std Deviation (1e-4) | 1.6 | 0.6 | 1.7 | 1.6 | 1.5 | 1.4 | 1.6 | 1.2 | 0.9 | 0.9 | 0.7 | 1.0 | 0.6 | 0.6 | 0.7 | 1.2 | 1.2 | 1.6 | 1.5 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.13 | -0.36 | -0.29 | -0.39 | -0.51 | -0.6 | -0.6 | -0.53 | -0.02 | 0.02 | 0.09 | -0.15 | 0.06 | 0.3 | 0.26 | 0.26 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 2.0 | 3.5 | 5.2 | 5.8 | 4.4 | 5.9 | 6.2 | 5.4 | 0 | 0 | 1.9 | 6.5 | 3.0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.1 | 4.0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | -2.6 | -3.2 | -2.6 | -1.9 | -1.8 | 0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.4 | 0.3 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | -3.4 | -4.2 | -7.0 | -3.5 | -2.2 | -2.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.1 | -4.0 | 0 | -2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | 0 | -0.7 | 0 |
Nucleolus | -1.3 | -3.1 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | -2.9 | -0.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.4 | 3.9 | 0 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 0 | 3.1 | 0 |
Vacuole | -5.5 | -6.9 | -10.5 | -10.9 | -14.3 | -13.6 | -14.4 | -14.1 | -16.1 | 1.2 | 2.2 | 1.2 | -0.5 | -0.3 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3991 | 1.7886 | 1.8048 | 1.6988 | 1.1971 | 1.7634 | 2.1573 | 3.0685 | 2.364 | 1.9166 | 2.1424 | 2.3611 | 2.0171 | 2.3442 | 2.1395 | 1.359 | 2.1252 | 1.9609 |
Actin | 0.0021 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.004 | 0 | 0.0077 | 0.0109 | 0.0031 | 0.0001 | 0.0046 | 0.0001 | 0.0057 | 0 | 0 | 0.0031 |
Bud | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0005 | 0.0008 | 0.0009 | 0.0005 | 0.0004 | 0.0052 | 0.0006 | 0.0003 | 0.0005 | 0.0013 | 0.0004 | 0.0001 | 0.0006 |
Bud Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0003 | 0.0011 | 0.0003 | 0.0012 | 0.0003 | 0.0008 | 0.0001 | 0 | 0.0004 |
Bud Site | 0.0004 | 0.0003 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0025 | 0.003 | 0.0013 | 0.0004 | 0.0198 | 0.0004 | 0.0007 | 0.0082 | 0.0015 | 0.0001 | 0.0001 | 0.0004 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0015 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.0143 | 0.0182 | 0.0278 | 0.0039 | 0.0116 | 0.0113 | 0.0302 | 0.0372 | 0.0124 | 0.022 | 0.0107 | 0.027 | 0.0083 | 0.1442 | 0.0642 | 0.0025 | 0.0128 | 0.0325 |
Cytoplasmic Foci | 0.0547 | 0.0372 | 0.052 | 0.0312 | 0.0301 | 0.0528 | 0.0593 | 0.0419 | 0.0894 | 0.0383 | 0.0607 | 0.0565 | 0.041 | 0.0558 | 0.0747 | 0.0305 | 0.0283 | 0.0419 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0028 | 0.0034 | 0.0007 | 0.0011 | 0.0023 | 0.0061 | 0.0037 | 0.0028 | 0.0011 | 0.0037 | 0.0024 | 0.0161 | 0.0056 | 0.0042 | 0.0006 | 0.0006 | 0.004 |
Endosome | 0.6353 | 0.6716 | 0.6478 | 0.7217 | 0.7446 | 0.6254 | 0.4992 | 0.4279 | 0.5538 | 0.5737 | 0.5807 | 0.608 | 0.4575 | 0.5064 | 0.4869 | 0.5972 | 0.6587 | 0.5803 |
Golgi | 0.0146 | 0.0132 | 0.0211 | 0.0283 | 0.0195 | 0.0216 | 0.0318 | 0.0088 | 0.0384 | 0.0312 | 0.0228 | 0.0284 | 0.0301 | 0.0119 | 0.0229 | 0.0172 | 0.0106 | 0.0262 |
Lipid Particles | 0.0231 | 0.0074 | 0.0114 | 0.0121 | 0.0024 | 0.0151 | 0.053 | 0.016 | 0.0432 | 0.0592 | 0.0368 | 0.0376 | 0.0731 | 0.0131 | 0.0333 | 0.0312 | 0.0025 | 0.0129 |
Mitochondria | 0.002 | 0.0027 | 0.0128 | 0.0243 | 0.0024 | 0.0095 | 0.0163 | 0.0077 | 0.0334 | 0.0087 | 0.002 | 0.0164 | 0.0725 | 0.0029 | 0.0146 | 0.0037 | 0.001 | 0.0141 |
None | 0.0116 | 0.0016 | 0.0051 | 0.0003 | 0.0003 | 0.0088 | 0.0518 | 0.0886 | 0.0261 | 0.0048 | 0.0047 | 0.001 | 0.0234 | 0.024 | 0.0421 | 0.0001 | 0.0001 | 0.0196 |
Nuclear Periphery | 0.0103 | 0.0038 | 0.0074 | 0.0012 | 0.0015 | 0.0051 | 0.0346 | 0.0064 | 0.0077 | 0.0022 | 0.0044 | 0.0037 | 0.0407 | 0.0051 | 0.0071 | 0.0016 | 0.0018 | 0.0055 |
Nucleolus | 0.0034 | 0.0004 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0013 | 0.005 | 0.0008 | 0.001 | 0.0016 | 0.0016 | 0.0008 | 0.0006 | 0.0007 | 0.0005 | 0.0002 | 0.0004 |
Nucleus | 0.0027 | 0.0007 | 0.0021 | 0.0003 | 0.0008 | 0.0011 | 0.0108 | 0.0054 | 0.001 | 0.0029 | 0.0024 | 0.0015 | 0.0024 | 0.0024 | 0.002 | 0.0003 | 0.0004 | 0.0021 |
Peroxisomes | 0.0036 | 0.0016 | 0.0013 | 0.0027 | 0.0008 | 0.0052 | 0.0271 | 0.0014 | 0.0227 | 0.009 | 0.0057 | 0.0036 | 0.0074 | 0.0009 | 0.0163 | 0.0071 | 0.0005 | 0.0024 |
Punctate Nuclear | 0.0033 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0035 | 0.0053 | 0.0005 | 0.0024 | 0.0009 | 0.01 | 0.0007 | 0.0023 | 0.0005 | 0.0017 | 0.0002 | 0.0001 | 0.0011 |
Vacuole | 0.1619 | 0.1572 | 0.1386 | 0.1164 | 0.1298 | 0.1413 | 0.1189 | 0.2833 | 0.1131 | 0.1492 | 0.159 | 0.1395 | 0.1239 | 0.1736 | 0.1684 | 0.1757 | 0.1864 | 0.1718 |
Vacuole Periphery | 0.0519 | 0.0807 | 0.0667 | 0.0558 | 0.0533 | 0.0945 | 0.0456 | 0.0599 | 0.0416 | 0.0834 | 0.0643 | 0.0703 | 0.0931 | 0.0427 | 0.0508 | 0.1308 | 0.0957 | 0.0803 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.1487 | 5.96 | 11.3021 | 11.73 | 10.0849 | 11.1057 | 10.2109 | 14.5742 | 15.1563 | 13.5481 |
Translational Efficiency | 1.2202 | 1.2693 | 0.7564 | 0.871 | 0.8894 | 0.919 | 1.068 | 0.5721 | 0.6463 | 0.8294 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
552 | 1927 | 298 | 1397 | 1206 | 1560 | 1409 | 1838 | 1758 | 3487 | 1707 | 3235 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 864.58 | 849.74 | 1026.82 | 987.66 | 633.68 | 847.22 | 920.15 | 874.93 | 706.18 | 848.61 | 938.77 | 923.61 |
Standard Deviation | 117.37 | 122.49 | 112.59 | 133.89 | 77.04 | 112.82 | 121.76 | 116.61 | 141.00 | 118.26 | 126.85 | 136.33 |
Intensity Change Log 2 | — | -0.024978 | 0.248112 | 0.192015 | — | 0.418982 | 0.538115 | 0.465413 | — | 0.179665 | 0.377943 | 0.314022 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001886 | 0.000027 | 0.000862 | 0.000190 | 0.000235 | 0.000053 | 0.000103 | 0.000063 | 0.000753 | 0.000039 | 0.000236 | 0.000118 |
Bud Neck | 0.009876 | 0.002410 | 0.010404 | 0.007157 | 0.010384 | 0.002739 | 0.008487 | 0.012683 | 0.010224 | 0.002557 | 0.008822 | 0.010296 |
Bud Site | 0.022436 | 0.007835 | 0.020248 | 0.022178 | 0.021046 | 0.003992 | 0.010718 | 0.010208 | 0.021482 | 0.006116 | 0.012382 | 0.015377 |
Cell Periphery | 0.000127 | 0.000066 | 0.000085 | 0.000074 | 0.000845 | 0.000138 | 0.000039 | 0.000101 | 0.000619 | 0.000098 | 0.000047 | 0.000089 |
Cytoplasm | 0.014296 | 0.019371 | 0.006167 | 0.019080 | 0.055195 | 0.004688 | 0.011708 | 0.009683 | 0.042353 | 0.012802 | 0.010741 | 0.013741 |
Cytoplasmic Foci | 0.041731 | 0.061797 | 0.089421 | 0.046285 | 0.156481 | 0.033103 | 0.040503 | 0.023854 | 0.120451 | 0.048960 | 0.049043 | 0.033541 |
Eisosomes | 0.000029 | 0.000009 | 0.000039 | 0.000012 | 0.000276 | 0.000017 | 0.000019 | 0.000028 | 0.000199 | 0.000013 | 0.000023 | 0.000021 |
Endoplasmic Reticulum | 0.001573 | 0.000708 | 0.001605 | 0.000777 | 0.008547 | 0.001743 | 0.001307 | 0.001894 | 0.006357 | 0.001171 | 0.001359 | 0.001412 |
Endosome | 0.296665* | 0.296693* | 0.344703* | 0.249654* | 0.212280* | 0.331603* | 0.299532* | 0.308865* | 0.238776* | 0.312311* | 0.307417* | 0.283295* |
Golgi | 0.019435 | 0.024393 | 0.042795 | 0.029134 | 0.058553 | 0.020005 | 0.015346 | 0.014606 | 0.046270 | 0.022430 | 0.020138 | 0.020880 |
Lipid Particles | 0.003084 | 0.001268 | 0.003184 | 0.000928 | 0.007762 | 0.005658 | 0.001565 | 0.003829 | 0.006293 | 0.003232 | 0.001847 | 0.002576 |
Mitochondria | 0.016173 | 0.014187 | 0.005525 | 0.005649 | 0.157697* | 0.023510 | 0.004896 | 0.012175 | 0.113260* | 0.018358 | 0.005006 | 0.009357 |
Mitotic Spindle | 0.008789 | 0.007989 | 0.079673 | 0.019335 | 0.000565 | 0.002002 | 0.020535 | 0.013896 | 0.003147 | 0.005310 | 0.030859 | 0.016245 |
None | 0.000189 | 0.000337 | 0.000137 | 0.000116 | 0.000881 | 0.000796 | 0.000185 | 0.001591 | 0.000663 | 0.000542 | 0.000177 | 0.000954 |
Nuclear Periphery | 0.002131 | 0.000675 | 0.003081 | 0.000936 | 0.000247 | 0.000725 | 0.002270 | 0.003695 | 0.000838 | 0.000697 | 0.002411 | 0.002504 |
Nuclear Periphery Foci | 0.001861 | 0.001839 | 0.013547 | 0.002618 | 0.000167 | 0.002691 | 0.004886 | 0.005817 | 0.000699 | 0.002220 | 0.006398 | 0.004436 |
Nucleolus | 0.004120 | 0.001222 | 0.007277 | 0.001544 | 0.000240 | 0.001810 | 0.007820 | 0.012457 | 0.001459 | 0.001485 | 0.007725 | 0.007744 |
Nucleus | 0.007677 | 0.001758 | 0.007383 | 0.002449 | 0.003426 | 0.001710 | 0.009421 | 0.016870 | 0.004760 | 0.001737 | 0.009065 | 0.010642 |
Peroxisomes | 0.006130 | 0.002091 | 0.021942 | 0.004174 | 0.004391 | 0.004123 | 0.006684 | 0.004499 | 0.004937 | 0.003000 | 0.009347 | 0.004359 |
Vacuole | 0.441559* | 0.342562* | 0.252679* | 0.490158* | 0.125102 | 0.347221* | 0.438797* | 0.433401* | 0.224467* | 0.344647* | 0.406305* | 0.457911* |
Vacuole Periphery | 0.100234* | 0.212763* | 0.089242 | 0.097552 | 0.175680* | 0.211673* | 0.115180* | 0.109784* | 0.151991* | 0.212276* | 0.110652* | 0.104502* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.88 | 0.94 | 1.70 | -3.71 | 2.65 | 5.08 | 3.21 | 4.82 | -0.76 | 2.70 | 2.29 | 1.57 | 2.02 | -3.91 | 2.50 |
Bud Neck | 5.49 | -0.65 | 1.45 | -7.96 | 2.35 | 7.39 | 1.30 | -1.76 | -10.44 | -3.53 | 9.32 | 1.01 | -0.37 | -13.04 | -1.67 |
Bud Site | 4.16 | 0.18 | -0.24 | -8.05 | -0.48 | 10.57 | 5.60 | 5.88 | -6.71 | 0.52 | 9.69 | 4.81 | 3.01 | -9.68 | -2.43 |
Cell Periphery | 2.79 | 1.84 | 1.75 | -1.38 | 0.08 | 6.67 | 7.66 | 6.86 | 1.27 | -2.31 | 7.11 | 7.84 | 7.04 | 0.20 | -2.92 |
Cytoplasm | -1.42 | 2.96 | -2.60 | -1.56 | -7.67 | 14.73 | 11.24 | 12.42 | -4.23 | 1.24 | 11.01 | 10.72 | 9.57 | -2.34 | -2.40 |
Cytoplasmic Foci | -5.26 | -6.69 | -1.11 | 5.35 | 6.49 | 27.38* | 25.86 | 30.65 | 4.61 | 8.43 | 19.88 | 19.15 | 25.09 | 8.63 | 7.35 |
Eisosomes | 5.70 | -2.13 | 4.38 | -2.21 | 6.34 | 7.11 | 7.04 | 6.80 | -5.51 | -4.23 | 7.41 | 6.95 | 7.05 | -6.90 | 1.35 |
Endoplasmic Reticulum | 4.00 | -0.28 | 3.22 | -0.86 | 3.08 | 7.49 | 8.03 | 7.43 | -0.55 | -2.27 | 8.40 | 7.88 | 7.97 | -1.71 | 0.22 |
Endosome | -0.13 | -2.30 | 4.28 | 6.40 | 5.93 | -12.49 | -9.12 | -10.79 | 2.64 | -1.37 | -10.22 | -7.76 | -5.67 | 5.25 | 3.11 |
Golgi | -1.86 | -4.70 | -2.77 | -1.54 | 3.07 | 12.31 | 14.73 | 14.70 | 3.46 | 0.43 | 10.26 | 11.23 | 11.01 | 1.63 | -0.41 |
Lipid Particles | 1.60 | -0.23 | 1.84 | 1.34 | 4.01 | 1.50 | 6.47 | 3.46 | 1.57 | -2.82 | 3.61 | 5.67 | 4.53 | 1.09 | -1.28 |
Mitochondria | 0.66 | 3.81 | 3.81 | 6.58 | -0.05 | 21.74* | 25.90 | 24.46 | 5.44 | -6.86 | 20.97* | 24.44 | 23.36 | 7.56 | -5.54 |
Mitotic Spindle | 0.36 | -6.38 | -3.47 | -4.47 | 5.31 | -3.36 | -8.16 | -7.96 | -7.01 | 2.38 | -2.17 | -9.60 | -8.51 | -7.25 | 4.60 |
None | -1.86 | 0.94 | 1.14 | 2.98 | 0.37 | 0.11 | 1.70 | -1.06 | -1.05 | -2.61 | 0.31 | 1.72 | -0.72 | -1.06 | -2.54 |
Nuclear Periphery | 1.59 | -0.77 | 1.19 | -2.19 | 2.63 | -4.42 | -6.32 | -6.22 | -5.31 | -1.89 | 0.51 | -3.98 | -3.91 | -5.66 | 0.05 |
Nuclear Periphery Foci | 0.14 | -3.38 | -1.08 | -1.26 | 3.08 | -3.34 | -5.34 | -5.75 | -2.52 | -0.49 | -3.37 | -5.96 | -5.79 | -3.08 | 1.84 |
Nucleolus | 2.85 | -2.05 | 2.43 | -0.96 | 3.97 | -3.45 | -10.07 | -10.79 | -8.74 | -2.99 | -0.12 | -8.73 | -8.64 | -8.92 | 0.47 |
Nucleus | 3.39 | -0.07 | 2.39 | -2.21 | 2.77 | 1.49 | -4.71 | -6.85 | -9.02 | -3.01 | 3.21 | -4.08 | -4.60 | -9.21 | -0.48 |
Peroxisomes | 4.10 | -4.12 | 1.97 | -3.41 | 4.76 | 0.20 | -1.95 | -0.23 | -0.45 | 2.01 | 2.87 | -3.78 | 0.91 | -2.70 | 4.62 |
Vacuole | 7.86 | 11.01 | -3.79 | -17.16 | -16.64 | -28.08 | -39.36 | -39.74 | -9.89 | 0.71 | -16.50 | -21.57 | -30.84 | -18.68 | -6.88 |
Vacuole Periphery | -16.22 | 1.35 | 0.90 | 20.79 | -0.84 | -4.95 | 10.11 | 10.95 | 17.78 | 0.85 | -11.34 | 8.61 | 10.68 | 26.95 | 1.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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