Standard name
Description Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.1 0.06 0.09 0.05 0.05 0.08 0 0 0 0.05 0.09 0.14 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.19 0 0.09 0 0.34 0.33 0.12 0.52 0.51 0.34 0.1 0 0 0.21 0 0 0 0 0 0
Nucleus 0.87 0.88 0.82 0.91 0.85 0.77 0.84 0.83 0.82 0.78 0.83 0.87 0.86 0.77 0.74 0.81 0.81 0.75 0.73 0.64 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 0.07 0.12 0.13 0.09 0 0.06 0 0.05 0 0 0.05 0.05 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0 0.07 0.09 0.16 0.15 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 0 2 2 3 2 5 5 0 0 0 0 0 5 2 2 15 2
Bud Neck 0 0 6 0 2 1 1 3 0 3 2 0 0 0 0 0 1 0 0 4 5
Bud Site 1 0 0 1 1 2 5 1 14 14 18 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 11 25 14 26 13 17 27 12 8 3 14 30 40 2 2 1 3 1 4 2
Endoplasmic Reticulum 0 2 2 0 3 0 1 0 1 0 1 0 1 1 1 1 0 1 0 0 2
Endosome 0 0 5 1 4 2 0 2 1 0 1 1 2 1 0 3 3 2 1 2 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 1
Mitochondria 30 59 5 22 9 82 113 41 166 160 88 29 7 13 4 1 7 6 1 5 6
Nucleus 112 280 207 231 239 186 287 281 261 246 216 243 274 227 14 109 281 215 135 217 177
Nuclear Periphery 1 2 2 0 0 0 1 1 4 1 2 0 0 0 0 0 0 0 0 1 0
Nucleolus 11 1 2 0 1 3 3 4 24 37 34 25 7 18 0 6 9 11 8 18 25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
SpindlePole 1 3 19 13 8 10 5 10 6 6 11 3 2 0 0 2 5 11 1 8 8
Vac/Vac Membrane 2 10 4 0 6 5 1 3 3 3 1 4 2 12 4 5 25 25 28 50 38
Unique Cell Count 129 318 253 253 282 243 340 337 320 315 260 280 318 293 19 135 346 288 185 339 287
Labelled Cell Count 166 369 277 282 299 306 437 376 494 483 382 320 327 312 25 135 346 288 185 339 287


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.8 5.6 5.1 5.9 4.5 4.7 5.3 4.4 4.1 4.5 5.3 5.8 5.7 7.6 4.9 5.1 5.6
Std Deviation (1e-4) 1.2 0.7 1.3 1.4 1.5 1.1 1.1 1.4 1.3 1.1 1.0 1.4 1.6 1.6 1.8 1.6 1.8 1.6
Intensity Change (Log2) -0.15 0.06 -0.32 -0.25 -0.09 -0.36 -0.47 -0.32 -0.09 0.04 0.01 0.44 -0.19 -0.14 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gis1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available