Standard name | |
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Human Ortholog | |
Description | S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated unfolded protein response; regulates HAC1 mRNA translocation, splicing and translation with KIN2 during ER stress; direct phosphorylation of the substrate Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the plasma membrane; activation loop phosphorylation (T302) required for full kinase activity; orthologous to MARK/PAR-1, AMP-activated protein kinase (AMPK) family members |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 2 | 18 | 7 | 7 | 2 | 9 | 6 | – | 9 | 4 | 18 | 8 | 8 | 4 | 1 | 1 | 0 | 26 | 6 | 65 | 27 | 28 | 21 |
Bud | 9 | 7 | 21 | 8 | 10 | 15 | 25 | 20 | – | 23 | 22 | 32 | 8 | 8 | 7 | 1 | 0 | 0 | 4 | 4 | 9 | 11 | 16 | 13 |
Bud Neck | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 31 | 8 | 24 | 27 | 18 | 10 |
Bud Site | 0 | 1 | 1 | 0 | 1 | 3 | 1 | 1 | – | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 1 | 30 | 33 | 35 | 22 | 48 | 42 | – | 34 | 22 | 61 | 45 | 45 | 30 | 4 | 0 | 0 | 7 | 1 | 30 | 11 | 9 | 9 |
Cytoplasm | 367 | 255 | 544 | 308 | 461 | 515 | 581 | 571 | – | 430 | 296 | 334 | 340 | 340 | 259 | 36 | 42 | 15 | 333 | 248 | 408 | 232 | 389 | 418 |
Endoplasmic Reticulum | 13 | 9 | 8 | 9 | 10 | 22 | 15 | 17 | – | 39 | 16 | 30 | 4 | 6 | 10 | 2 | 4 | 1 | 4 | 1 | 20 | 6 | 9 | 9 |
Endosome | 0 | 0 | 6 | 2 | 13 | 5 | 12 | 6 | – | 0 | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 11 | 2 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 2 |
Mitochondria | 0 | 13 | 10 | 15 | 40 | 124 | 153 | 160 | – | 203 | 163 | 174 | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 6 | 14 | 12 | 11 | 10 |
Nucleus | 0 | 1 | 1 | 2 | 2 | 3 | 3 | 6 | – | 4 | 0 | 1 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | – | 5 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 6 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 2 | 1 | 0 | 2 | 4 | 1 | – | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 7 | 0 | 3 | 0 | 1 | 1 | – | 1 | 0 | 4 | 1 | 1 | 0 | 3 | 0 | 2 | 0 | 1 | 4 | 0 | 2 | 3 |
Vac/Vac Membrane | 0 | 1 | 6 | 3 | 17 | 10 | 96 | 86 | – | 54 | 45 | 66 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 2 | 5 | 7 | 3 |
Unique Cell Count | 391 | 275 | 594 | 335 | 524 | 619 | 800 | 789 | 657 | 474 | 575 | 355 | 354 | 278 | 42 | 46 | 17 | 428 | 294 | 602 | 342 | 509 | 518 | |
Labelled Cell Count | 412 | 292 | 658 | 388 | 600 | 724 | 949 | 923 | 805 | 577 | 739 | 407 | 408 | 313 | 51 | 51 | 19 | 428 | 294 | 602 | 342 | 509 | 518 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 5.5 | 5.5 | 5.8 | 6.2 | 5.9 | 6.2 | 6.4 | – | 5.7 | 5.8 | 6.2 | 7.5 | 7.5 | 7.7 | 5.9 | 5.6 | 7.6 | 7.0 | 7.2 | 7.8 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.4 | 1.7 | 1.2 | 1.5 | 1.5 | – | 1.5 | 1.5 | 1.7 | 0.8 | 0.8 | 0.9 | 0.9 | 1.0 | 1.4 | 1.3 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.17 | 0.1 | 0.15 | 0.22 | – | 0.04 | 0.08 | 0.17 | 0.44 | 0.44 | 0.48 | 0.09 | 0.02 | 0.46 | 0.34 | 0.38 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -1.9 | -3.7 | -2.6 | -3.2 | – | -2.0 | -2.5 | 0.1 | -0.7 | -0.7 | -1.4 | 0 | 0 | 0 |
Bud | -1.0 | -1.7 | -1.1 | -0.4 | -1.1 | – | 0 | 0.9 | 1.7 | -1.1 | -1.1 | -0.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.8 | 1.2 | -1.3 | 0.8 | 0.2 | – | 0.1 | -0.3 | 3.5 | 4.2 | 4.2 | 3.1 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -2.0 | -4.4 | -8.9 | -8.9 | – | -11.1 | -11.5 | -13.2 | 2.5 | 2.6 | 0.8 | -1.3 | -0.1 | -0.5 |
Endoplasmic Reticulum | 1.5 | 0.7 | 2.5 | 0.8 | 1.1 | – | 4.3 | 2.2 | 3.7 | 0 | 0.4 | 2.2 | 0 | 0 | 0 |
Endosome | 0 | 1.9 | -0.4 | 0.8 | -0.5 | – | 0 | 0.7 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.5 | 4.8 | 10.2 | 10.0 | 10.4 | – | 13.7 | 14.4 | 13.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.6 | 0.9 | 7.8 | 7.3 | – | 6.0 | 6.5 | 7.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6763 | 3.6131 | 3.2294 | 2.8124 | 3.2517 | 3.3916 | 3.5901 | 4.5922 | 4.1554 | 3.7797 | 3.5354 | 4.3858 | 2.1365 | 3.5758 | 3.0248 | 3.0318 | 2.9109 | 3.1307 |
Actin | 0.0548 | 0.011 | 0.039 | 0.0134 | 0.1438 | 0.0255 | 0.0145 | 0.0126 | 0.0252 | 0.0473 | 0.0225 | 0.0142 | 0.1293 | 0.0471 | 0.0236 | 0.023 | 0.1154 | 0.0066 |
Bud | 0.0061 | 0.0051 | 0.0423 | 0.0053 | 0.0044 | 0.0015 | 0.0099 | 0.0044 | 0.0409 | 0.0319 | 0.0023 | 0.0014 | 0.0209 | 0.0009 | 0.0311 | 0.0306 | 0.0171 | 0.0011 |
Bud Neck | 0.0146 | 0.0011 | 0.0119 | 0.001 | 0.0052 | 0.2063 | 0.0099 | 0.0024 | 0.0015 | 0.0014 | 0.0217 | 0.2184 | 0.0027 | 0.0039 | 0.0008 | 0.0003 | 0.0024 | 0.2389 |
Bud Periphery | 0.0191 | 0.0068 | 0.1027 | 0.0118 | 0.009 | 0.0029 | 0.0133 | 0.0056 | 0.075 | 0.0921 | 0.0038 | 0.0012 | 0.0231 | 0.0005 | 0.0718 | 0.2932 | 0.0309 | 0.0016 |
Bud Site | 0.0341 | 0.039 | 0.0287 | 0.0012 | 0.0045 | 0.0018 | 0.0376 | 0.0941 | 0.0193 | 0.0143 | 0.0046 | 0.0018 | 0.0419 | 0.1215 | 0.0033 | 0.0012 | 0.003 | 0.0004 |
Cell Periphery | 0.0085 | 0.0023 | 0.0039 | 0.0025 | 0.0033 | 0.0018 | 0.0079 | 0.0062 | 0.0031 | 0.0044 | 0.0027 | 0.0016 | 0.0048 | 0.0028 | 0.002 | 0.0102 | 0.0045 | 0.0028 |
Cytoplasm | 0.6293 | 0.8329 | 0.6178 | 0.8494 | 0.6165 | 0.6705 | 0.7389 | 0.7844 | 0.6881 | 0.6591 | 0.829 | 0.6379 | 0.6572 | 0.7483 | 0.6438 | 0.5889 | 0.778 | 0.6804 |
Cytoplasmic Foci | 0.0221 | 0.0053 | 0.0226 | 0.0127 | 0.0276 | 0.0075 | 0.013 | 0.008 | 0.0089 | 0.0064 | 0.005 | 0.0132 | 0.0215 | 0.0091 | 0.0223 | 0.0038 | 0.0086 | 0.0021 |
Eisosomes | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0021 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0082 | 0.0029 | 0.0039 | 0.0072 | 0.007 | 0.0019 | 0.0051 | 0.0025 | 0.003 | 0.0082 | 0.0037 | 0.002 | 0.0046 | 0.0028 | 0.0036 | 0.006 | 0.0064 | 0.0022 |
Endosome | 0.0175 | 0.0014 | 0.0107 | 0.0113 | 0.0368 | 0.0033 | 0.0009 | 0.001 | 0.0024 | 0.0028 | 0.0012 | 0.0018 | 0.029 | 0.0006 | 0.0134 | 0.0013 | 0.0017 | 0.0009 |
Golgi | 0.0077 | 0.0007 | 0.0075 | 0.0032 | 0.0189 | 0.0021 | 0.0007 | 0.0008 | 0.0014 | 0.0022 | 0.0009 | 0.0006 | 0.006 | 0.001 | 0.0071 | 0.0007 | 0.0019 | 0.0002 |
Lipid Particles | 0.0181 | 0.0002 | 0.0073 | 0.0021 | 0.0141 | 0.0014 | 0.0006 | 0.0002 | 0.0005 | 0.0007 | 0.0002 | 0.0003 | 0.0147 | 0.0007 | 0.0159 | 0.0005 | 0.0006 | 0.0001 |
Mitochondria | 0.0131 | 0.0003 | 0.0126 | 0.0124 | 0.0096 | 0.0007 | 0.0002 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0031 | 0.0001 | 0.0003 | 0.0003 |
None | 0.0789 | 0.083 | 0.0706 | 0.0517 | 0.0589 | 0.0576 | 0.1425 | 0.0741 | 0.1224 | 0.12 | 0.0981 | 0.1021 | 0.009 | 0.0555 | 0.1308 | 0.0375 | 0.0266 | 0.0594 |
Nuclear Periphery | 0.0115 | 0.0013 | 0.0012 | 0.0026 | 0.0139 | 0.004 | 0.0005 | 0.0003 | 0.0006 | 0.0012 | 0.0006 | 0.0004 | 0.0096 | 0.0007 | 0.0005 | 0.0006 | 0.0006 | 0.0011 |
Nucleolus | 0.0013 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.012 | 0.0026 | 0.0016 | 0.0022 | 0.0034 | 0.0026 | 0.0011 | 0.0011 | 0.0015 | 0.0023 | 0.0014 | 0.0008 | 0.0011 | 0.0008 | 0.0006 | 0.0005 | 0.0004 | 0.0011 |
Peroxisomes | 0.0161 | 0.0011 | 0.0089 | 0.0036 | 0.0136 | 0.0021 | 0.0004 | 0.0007 | 0.0008 | 0.0006 | 0.0002 | 0.0004 | 0.0036 | 0.0009 | 0.0231 | 0 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0217 | 0.0013 | 0.0031 | 0.0022 | 0.0055 | 0.0048 | 0.0009 | 0.0004 | 0.0012 | 0.0007 | 0.0007 | 0.0011 | 0.018 | 0.0021 | 0.0009 | 0.0002 | 0.0004 | 0.0001 |
Vacuole | 0.0037 | 0.0012 | 0.0025 | 0.0031 | 0.0018 | 0.0007 | 0.0011 | 0.0008 | 0.001 | 0.0023 | 0.001 | 0.0004 | 0.0009 | 0.0002 | 0.0015 | 0.0012 | 0.0005 | 0.0004 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0006 | 0.0007 | 0.0014 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.2108 | 13.2752 | 21.076 | 35.4018 | 22.1644 | 35.2066 | 26.3768 | 35.3944 | 37.886 | 34.4998 |
Translational Efficiency | 0.7082 | 0.8959 | 0.6985 | 0.5924 | 0.6913 | 0.6432 | 0.5328 | 0.4343 | 0.5456 | 0.5353 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
189 | 997 | 1877 | 1388 | 1172 | 1653 | 1598 | 2094 | 1361 | 2650 | 3475 | 3482 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 974.24 | 828.21 | 1057.47 | 1050.68 | 722.11 | 817.55 | 924.33 | 962.41 | 757.12 | 821.56 | 996.24 | 997.60 |
Standard Deviation | 145.81 | 116.89 | 129.40 | 184.99 | 126.22 | 108.56 | 111.75 | 133.79 | 155.80 | 111.89 | 138.53 | 162.09 |
Intensity Change Log 2 | — | -0.234281 | 0.118268 | 0.108974 | — | 0.179088 | 0.356189 | 0.414433 | — | -0.043680 | 0.224378 | 0.246978 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.014009 | 0.001660 | 0.009257 | 0.013339 | 0.008205 | 0.002684 | 0.002193 | 0.002397 | 0.009011 | 0.002299 | 0.006008 | 0.006759 |
Bud Neck | 0.023194 | 0.037091 | 0.036349 | 0.024143 | 0.091113 | 0.074193 | 0.041976 | 0.037958 | 0.081681 | 0.060234 | 0.038937 | 0.032451 |
Bud Site | 0.080706 | 0.044434 | 0.238093* | 0.173488* | 0.241618* | 0.063593 | 0.059138 | 0.148936* | 0.219272* | 0.056385 | 0.155800* | 0.158723* |
Cell Periphery | 0.001102 | 0.000296 | 0.000711 | 0.001714 | 0.008776 | 0.000668 | 0.000369 | 0.000459 | 0.007711 | 0.000528 | 0.000554 | 0.000959 |
Cytoplasm | 0.294969* | 0.534118* | 0.545296* | 0.431207* | 0.434819* | 0.516468* | 0.694658* | 0.556153* | 0.415398* | 0.523109* | 0.613981* | 0.506347* |
Cytoplasmic Foci | 0.065882 | 0.165555 | 0.038091 | 0.031904 | 0.113576 | 0.167681 | 0.015481 | 0.020587 | 0.106953 | 0.166881 | 0.027694 | 0.025098 |
Eisosomes | 0.000271 | 0.000264 | 0.000161 | 0.000424 | 0.002050 | 0.000817 | 0.000153 | 0.000131 | 0.001803 | 0.000609 | 0.000157 | 0.000248 |
Endoplasmic Reticulum | 0.003317 | 0.000748 | 0.001449 | 0.003317 | 0.004480 | 0.000652 | 0.000409 | 0.000609 | 0.004318 | 0.000688 | 0.000971 | 0.001689 |
Endosome | 0.011485 | 0.014351 | 0.001908 | 0.007316 | 0.007098 | 0.014017 | 0.001686 | 0.004052 | 0.007707 | 0.014143 | 0.001806 | 0.005353 |
Golgi | 0.017471 | 0.003326 | 0.000665 | 0.005793 | 0.007683 | 0.007031 | 0.000350 | 0.003010 | 0.009043 | 0.005637 | 0.000520 | 0.004120 |
Lipid Particles | 0.002361 | 0.002363 | 0.000980 | 0.003469 | 0.004997 | 0.003166 | 0.000403 | 0.000504 | 0.004631 | 0.002864 | 0.000715 | 0.001686 |
Mitochondria | 0.000884 | 0.002879 | 0.000260 | 0.003755 | 0.013220 | 0.005288 | 0.001016 | 0.002083 | 0.011506 | 0.004381 | 0.000608 | 0.002750 |
Mitotic Spindle | 0.016306 | 0.003410 | 0.001448 | 0.016865 | 0.000635 | 0.004027 | 0.002097 | 0.013989 | 0.002811 | 0.003795 | 0.001746 | 0.015136 |
None | 0.000498 | 0.000756 | 0.000966 | 0.001054 | 0.006389 | 0.000998 | 0.001041 | 0.002492 | 0.005571 | 0.000907 | 0.001001 | 0.001919 |
Nuclear Periphery | 0.000300 | 0.000126 | 0.000079 | 0.000356 | 0.000073 | 0.000166 | 0.000082 | 0.000177 | 0.000105 | 0.000151 | 0.000081 | 0.000248 |
Nuclear Periphery Foci | 0.002342 | 0.000796 | 0.002302 | 0.002648 | 0.000439 | 0.000459 | 0.000261 | 0.000423 | 0.000703 | 0.000586 | 0.001363 | 0.001310 |
Nucleolus | 0.000115 | 0.000426 | 0.000093 | 0.000116 | 0.000310 | 0.000530 | 0.000107 | 0.000212 | 0.000283 | 0.000491 | 0.000100 | 0.000174 |
Nucleus | 0.074123 | 0.052015 | 0.057150 | 0.076809 | 0.024759 | 0.044106 | 0.093920 | 0.039461 | 0.031614 | 0.047082 | 0.074059 | 0.054348 |
Peroxisomes | 0.003998 | 0.006868 | 0.003139 | 0.003758 | 0.005867 | 0.010341 | 0.002046 | 0.001711 | 0.005607 | 0.009035 | 0.002637 | 0.002527 |
Vacuole | 0.383881* | 0.127520 | 0.061444 | 0.196430 | 0.021947 | 0.081569 | 0.082498 | 0.161834 | 0.072208 | 0.098857 | 0.071125 | 0.175624 |
Vacuole Periphery | 0.002788 | 0.000997 | 0.000157 | 0.002095 | 0.001945 | 0.001544 | 0.000114 | 0.002823 | 0.002062 | 0.001339 | 0.000137 | 0.002533 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.67 | -0.19 | -0.20 | -7.26 | -0.02 | 4.77 | 5.46 | 5.61 | 2.34 | 0.42 | 5.27 | 2.14 | 3.12 | -5.18 | 1.50 |
Bud Neck | -3.77 | -3.62 | -0.14 | 5.60 | 7.39 | 2.69 | 9.96 | 11.99 | 10.83 | 2.47 | 3.98 | 10.00 | 12.64 | 12.23 | 5.59 |
Bud Site | 1.37 | -13.91 | -4.26 | -9.30 | 13.27 | 20.65* | 22.47* | 15.38* | -7.19 | -10.04 | 21.08 | 9.83 | 14.51 | -11.18 | 6.63 |
Cell Periphery | 1.84 | 0.12 | -1.71 | -7.72 | -3.99 | 8.08 | 8.43 | 8.42 | 6.66 | -0.35 | 8.22 | 8.23 | 8.06 | -2.39 | -2.68 |
Cytoplasm | -13.05 | -14.28 | -4.80 | 14.35 | 17.57 | -7.71 | -22.53 | -7.41 | 0.37 | 16.99 | -11.73 | -20.79 | -4.51 | 9.17 | 20.84 |
Cytoplasmic Foci | -15.51 | 2.20 | 5.29 | 25.71 | 7.67 | -9.08 | 22.42 | 21.93 | 33.99 | -1.43 | -12.33 | 19.55 | 21.97 | 42.57 | 6.64 |
Eisosomes | -0.92 | 1.79 | -2.35 | -1.60 | -4.38 | 7.24 | 11.80 | 11.98 | 12.62 | 1.97 | 8.23 | 11.71 | 11.36 | 10.61 | -2.62 |
Endoplasmic Reticulum | 3.93 | 2.13 | -0.90 | -11.95 | -7.27 | 7.62 | 7.91 | 7.45 | -1.39 | -3.86 | 8.11 | 7.03 | 5.61 | -11.06 | -5.83 |
Endosome | -3.48 | 3.92 | 2.62 | 6.99 | -2.41 | -7.01 | 7.06 | 5.99 | 13.13 | -2.31 | -7.61 | 8.80 | 6.28 | 13.99 | -3.43 |
Golgi | 1.35 | 1.96 | 1.57 | 1.04 | -1.93 | 0.76 | 13.30 | 7.45 | 5.82 | -2.50 | 2.55 | 9.55 | 6.67 | 5.44 | -3.00 |
Lipid Particles | -0.94 | 2.82 | -1.95 | -1.42 | -4.02 | 2.40 | 6.13 | 5.91 | 11.11 | -4.42 | 2.66 | 5.87 | 4.50 | 4.99 | -3.96 |
Mitochondria | -2.53 | 1.40 | -1.94 | 1.16 | -2.49 | 4.50 | 7.86 | 7.42 | 4.46 | -1.54 | 4.80 | 8.06 | 7.23 | 4.30 | -3.19 |
Mitotic Spindle | 0.95 | 1.34 | -0.25 | -3.00 | -4.74 | -3.44 | -1.57 | -7.24 | -3.92 | -5.81 | -1.87 | 0.19 | -6.56 | -4.92 | -7.67 |
None | -1.83 | -3.30 | -3.40 | -1.79 | -0.21 | 4.21 | 4.24 | 3.39 | -2.36 | -2.58 | 4.23 | 4.19 | 3.61 | -2.62 | -2.62 |
Nuclear Periphery | 5.86 | 6.71 | -3.46 | -11.41 | -12.77 | -1.36 | -4.29 | -15.24 | -2.73 | -14.22 | -0.95 | -0.62 | -16.33 | -5.85 | -18.86 |
Nuclear Periphery Foci | 3.89 | -1.39 | -0.38 | -3.95 | 0.85 | -0.17 | 2.05 | -1.83 | -0.70 | -4.87 | 0.68 | -6.36 | -3.65 | -3.81 | 1.69 |
Nucleolus | -2.54 | -0.01 | -0.08 | 2.53 | -0.09 | -1.08 | 2.45 | 1.43 | 2.09 | -1.17 | -1.44 | 2.55 | 1.81 | 3.02 | -1.25 |
Nucleus | 2.35 | 0.86 | -2.00 | -11.46 | -7.64 | -9.07 | -23.31 | -15.59 | -7.32 | 11.79 | -6.59 | -19.85 | -17.08 | -13.32 | 3.57 |
Peroxisomes | -3.38 | -0.45 | 0.34 | 4.95 | 1.58 | -4.25 | 7.13 | 8.78 | 9.75 | 1.66 | -4.47 | 6.19 | 7.72 | 10.83 | 2.87 |
Vacuole | 15.11 | 17.66 | 5.27 | -23.34 | -31.93 | -20.05 | -22.52 | -39.18 | -27.18 | -20.10 | -2.87 | -2.18 | -28.73 | -35.46 | -36.73 |
Vacuole Periphery | 0.62 | 1.69 | 0.78 | 0.36 | -2.31 | 1.29 | 9.26 | 0.53 | -0.16 | -2.76 | 2.05 | 8.63 | 1.40 | 0.03 | -3.34 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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