Standard name
Human Ortholog
Description Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0.07 0.07 0.06 0.08 0.07 0.05 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.55 0.67 0.69 0.54 0.51 0.35 0.33 0.33 0.24 0.23 0.25 0.21 0.96 0.96 0.97 0.88 – – 0.16 0.27 0.08 0.27 0.27 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.07 0.07 0.07 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.51 0.31 0.15 0.45 0.45 0.71 0.74 0.68 0.78 0.83 0.85 0.86 0 0 0 0.15 – – 0.58 0.47 0.63 0.47 0.47 0.48
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.09 0.05 0 0.07 0.09 0.07 0.08 0 0.08 0 0 0 0 – – 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0.06 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 0 0 0 0 0 0 2 1 0 0 – – 3 8 14 11 13 23
Bud 4 5 7 7 9 27 29 23 31 20 18 11 2 0 0 0 – – 1 1 2 7 7 16
Bud Neck 1 1 5 1 4 4 2 3 3 1 0 3 2 13 2 0 – – 2 2 3 4 11 12
Bud Site 0 0 1 1 0 2 8 7 6 9 9 9 0 0 0 0 – – – – – – – –
Cell Periphery 0 2 2 3 1 0 4 1 1 1 1 1 1 2 1 1 – – 0 1 2 2 6 8
Cytoplasm 106 144 187 97 110 140 143 131 90 67 92 72 482 351 480 30 – – 39 68 28 119 153 132
Endoplasmic Reticulum 7 3 2 1 3 1 1 1 4 0 0 2 1 0 1 1 – – 3 1 11 2 2 5
Endosome 1 8 7 1 8 0 0 4 0 0 1 0 8 4 10 0 – – 17 17 27 19 26 33
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 – – 1 0 4 0 2 3
Mitochondria 99 67 40 81 97 283 324 271 290 240 319 297 3 0 2 5 – – 143 120 240 205 263 289
Nucleus 0 0 0 0 3 2 3 5 7 5 5 3 0 3 0 1 – – 0 0 0 2 3 4
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 – – 0 0 0 2 0 2
Nucleolus 0 1 1 0 0 0 0 1 0 0 2 0 0 0 0 0 – – 0 0 0 2 1 2
Peroxisomes 0 0 8 2 1 1 2 2 0 0 0 1 2 3 3 0 – – 1 0 11 2 2 3
SpindlePole 5 3 31 16 10 14 29 34 25 23 14 26 4 2 1 0 – – 9 9 18 18 21 26
Vac/Vac Membrane 5 2 3 1 4 10 4 9 5 4 6 11 6 3 4 0 – – 15 15 7 25 32 27
Unique Cell Count 194 214 272 179 214 399 438 398 374 289 374 345 503 365 494 34 248 255 382 438 566 607
Labelled Cell Count 228 238 296 212 251 484 549 492 462 370 468 436 513 382 504 39 248 255 382 438 566 607


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.7 4.0 3.5 4.1 3.2 3.1 3.3 3.0 2.9 2.4 2.6 4.9 5.0 4.9 4.9 4.8 4.7 5.1 5.1 5.7
Std Deviation (1e-4) 0.4 0.6 1.2 1.3 2.0 1.8 1.6 1.6 1.3 1.1 1.0 1.0 0.9 0.8 1.1 1.3 2.3 1.1 1.2 1.1 1.7
Intensity Change (Log2) – – – -0.19 0.03 -0.32 -0.37 -0.31 -0.43 -0.5 -0.73 -0.63 0.28 0.31 0.26 0.28 0.25 0.21 0.34 0.33 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2931 2.0732 1.6591 2.0235 1.8314 1.9634 0.2944 0.0719 0.1494 -0.1081 -0.2514 0.1889 -1.4366 -0.9514 -1.2461 -0.7502 -1.1374 -1.0243
Actin 0.0104 0.0075 0.0062 0.0016 0.0335 0.005 0.0306 0.011 0.0164 0.0061 0.0296 0.017 0.0275 0.0113 0.0088 0.0042 0.0477 0.0186
Bud 0.0008 0.0115 0.0052 0.0037 0.0006 0.0027 0.0031 0.006 0.012 0.0037 0.0025 0.0027 0.0039 0.0027 0.0021 0.002 0.0009 0.007
Bud Neck 0.0034 0.0039 0.0176 0.0018 0.0398 0.0136 0.017 0.0011 0.0066 0.0167 0.0023 0.0104 0.008 0.0021 0.0027 0.0111 0.0008 0.0078
Bud Periphery 0.0028 0.0083 0.0105 0.0115 0.0013 0.0031 0.0105 0.0112 0.0256 0.0152 0.0088 0.0098 0.0096 0.0057 0.0049 0.0042 0.002 0.0085
Bud Site 0.0058 0.085 0.0167 0.0017 0.0068 0.0015 0.0127 0.0252 0.031 0.0104 0.0026 0.0021 0.0147 0.0249 0.0151 0.0039 0.0011 0.0013
Cell Periphery 0.0029 0.0031 0.0035 0.0006 0.0005 0.0014 0.0014 0.0017 0.0029 0.0017 0.0007 0.0021 0.0023 0.0016 0.0018 0.0008 0.0004 0.0007
Cytoplasm 0.111 0.1672 0.1221 0.1006 0.0736 0.153 0.0988 0.1445 0.131 0.1093 0.1352 0.1626 0.1008 0.1588 0.1404 0.1107 0.1614 0.1852
Cytoplasmic Foci 0.1068 0.1105 0.1001 0.0939 0.0772 0.1085 0.129 0.1241 0.1323 0.1768 0.1665 0.1507 0.185 0.1269 0.1066 0.1185 0.122 0.1631
Eisosomes 0.0021 0.0007 0.0014 0.0004 0.0006 0.0006 0.0006 0.001 0.0012 0.0008 0.0003 0.0004 0.0012 0.0012 0.0018 0.0002 0.0008 0.0005
Endoplasmic Reticulum 0.0067 0.0049 0.0026 0.0038 0.0063 0.0038 0.0082 0.0049 0.0053 0.0062 0.0043 0.0056 0.0034 0.0043 0.0054 0.003 0.0045 0.0034
Endosome 0.0572 0.0512 0.0421 0.0805 0.0472 0.0486 0.1436 0.0767 0.0832 0.1165 0.1124 0.0696 0.1072 0.1025 0.0806 0.084 0.0918 0.0864
Golgi 0.0114 0.0116 0.0127 0.0132 0.0136 0.0116 0.0215 0.0209 0.0236 0.0196 0.033 0.0164 0.0287 0.0168 0.0248 0.0126 0.0288 0.0199
Lipid Particles 0.0223 0.0067 0.0123 0.0059 0.0085 0.0181 0.0321 0.0102 0.0209 0.0073 0.0123 0.0126 0.0242 0.0064 0.0084 0.0152 0.0163 0.0135
Mitochondria 0.0361 0.0289 0.0819 0.0728 0.0389 0.0726 0.0656 0.0694 0.1235 0.1097 0.1609 0.0934 0.0584 0.0373 0.1012 0.091 0.0708 0.0781
None 0.5811 0.4584 0.5187 0.5715 0.6087 0.5166 0.359 0.4458 0.3319 0.345 0.2763 0.3851 0.365 0.4559 0.4669 0.4848 0.3976 0.3628
Nuclear Periphery 0.0018 0.0018 0.0032 0.002 0.0007 0.0013 0.0119 0.003 0.0028 0.0053 0.0018 0.0078 0.0013 0.0011 0.0017 0.002 0.0027 0.0028
Nucleolus 0.0034 0.0018 0.0019 0.0011 0.0004 0.0016 0.0046 0.0019 0.002 0.0014 0.0016 0.0013 0.0037 0.0014 0.0007 0.0011 0.0008 0.0006
Nucleus 0.0029 0.0039 0.0033 0.0025 0.0014 0.0022 0.0066 0.0036 0.0041 0.0025 0.0025 0.0044 0.0025 0.0017 0.0015 0.0033 0.0016 0.0058
Peroxisomes 0.0136 0.0128 0.0252 0.0112 0.0368 0.0229 0.0152 0.022 0.0254 0.0218 0.0207 0.028 0.0371 0.0228 0.0144 0.0127 0.0361 0.0202
Punctate Nuclear 0.0067 0.0044 0.0051 0.0075 0.0014 0.0041 0.0129 0.004 0.0045 0.0025 0.0036 0.008 0.0037 0.0013 0.0012 0.0048 0.0018 0.0068
Vacuole 0.0089 0.0137 0.0064 0.0091 0.0015 0.0055 0.012 0.0096 0.0104 0.0139 0.0163 0.0067 0.0096 0.0112 0.0068 0.0239 0.0073 0.005
Vacuole Periphery 0.0017 0.0023 0.0015 0.003 0.0007 0.0016 0.003 0.0021 0.0032 0.0078 0.006 0.0033 0.0022 0.0022 0.0022 0.0058 0.0028 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2076 550 148 103 1422 1774 234 534 3498 2324 382 637

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.78 696.39 850.53 867.12 647.36 714.25 860.02 834.08 613.78 710.02 856.34 839.42
Standard Deviation 63.18 89.83 81.86 99.58 72.85 89.73 97.67 132.61 72.80 90.08 91.99 128.43
Intensity Change Log 2 — 0.237274 0.525741 0.553611 — 0.141861 0.409802 0.365618 — 0.188175 0.466286 0.458378

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000838 0.001307 0.001910 0.007911 0.000508 0.000789 0.000525 0.001336 0.000704 0.000912 0.001061 0.002399
Bud Neck 0.036816 0.050172 0.016360 0.009784 0.030555 0.058145 0.006719 0.020942 0.034271 0.056258 0.010455 0.019138
Bud Site 0.016800 0.039493 0.009376 0.053335 0.009350 0.024725 0.004663 0.036154 0.013771 0.028220 0.006489 0.038932
Cell Periphery 0.000362 0.000243 0.000374 0.000462 0.000264 0.000148 0.000226 0.000154 0.000322 0.000171 0.000283 0.000204
Cytoplasm 0.118760* 0.104129* 0.197908* 0.184211* 0.205224* 0.101064* 0.212084* 0.320812* 0.153909* 0.101789* 0.206592* 0.298724*
Cytoplasmic Foci 0.290823* 0.305648* 0.104920 0.182272* 0.383268* 0.293658* 0.093084 0.131761 0.328404* 0.296496* 0.097669 0.139928
Eisosomes 0.000646 0.000207 0.000233 0.000336 0.000377 0.000222 0.000167 0.000112 0.000537 0.000219 0.000193 0.000148
Endoplasmic Reticulum 0.002247 0.000567 0.007222 0.011926 0.001542 0.000835 0.004078 0.002206 0.001960 0.000772 0.005296 0.003778
Endosome 0.027363 0.094411 0.016287 0.023739 0.039273 0.080549 0.016630 0.029635 0.032204 0.083830 0.016498 0.028682
Golgi 0.059858 0.060022 0.001072 0.003970 0.025547 0.059211 0.001395 0.005760 0.045910 0.059403 0.001270 0.005471
Lipid Particles 0.033719 0.011626 0.015763 0.015569 0.034290 0.012514 0.014751 0.003543 0.033951 0.012304 0.015144 0.005487
Mitochondria 0.305794* 0.125857* 0.001932 0.001289 0.092944 0.191645* 0.001183 0.006421 0.219267* 0.176076* 0.001473 0.005591
Mitotic Spindle 0.001515 0.014433 0.003443 0.007161 0.005894 0.014515 0.008013 0.044693 0.003295 0.014496 0.006242 0.038624
None 0.002532 0.001549 0.002499 0.000563 0.003392 0.001251 0.005550 0.001611 0.002882 0.001322 0.004368 0.001442
Nuclear Periphery 0.000515 0.000264 0.002816 0.001170 0.001266 0.000491 0.002719 0.001634 0.000820 0.000437 0.002757 0.001559
Nuclear Periphery Foci 0.000287 0.000366 0.004516 0.006775 0.000532 0.000444 0.004455 0.002186 0.000387 0.000426 0.004479 0.002928
Nucleolus 0.001783 0.003423 0.004360 0.001312 0.002277 0.002155 0.004354 0.001545 0.001984 0.002455 0.004357 0.001507
Nucleus 0.029699 0.026651 0.486032* 0.155614* 0.077191 0.024748 0.526860* 0.142717* 0.049006 0.025198 0.511042* 0.144803*
Peroxisomes 0.046975 0.105389* 0.017135 0.058782 0.047284 0.054899 0.005252 0.027286 0.047101 0.066848 0.009856 0.032379
Vacuole 0.015879 0.046887 0.104817 0.273017* 0.034043 0.068145 0.085678 0.212652* 0.023263 0.063114 0.093093 0.222413*
Vacuole Periphery 0.006790 0.007352 0.001024 0.000801 0.004979 0.009846 0.001613 0.006842 0.006054 0.009256 0.001385 0.005865

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.76 -2.37 -2.29 -2.02 -1.65 -2.29 -1.80 -4.08 -2.64 -2.79 -1.83 -2.52 -3.68 -3.02 -2.03
Bud Neck -2.66 4.10 11.70 8.97 1.07 -7.85 7.59 4.58 11.15 -3.14 -7.62 8.46 7.97 13.07 -1.95
Bud Site -5.72 3.21 -1.61 0.57 -2.38 -8.75 1.34 -5.21 -1.07 -5.44 -8.48 3.85 -4.43 -0.40 -5.93
Cell Periphery 2.67 -0.42 0.00 -2.14 0.34 3.68 0.45 3.60 0.21 4.45 5.89 0.77 5.12 -0.61 5.44
Cytoplasm 1.59 -4.16 -3.04 -3.51 0.09 14.78 0.12 -6.18 -14.95 -4.84 10.73 -4.16 -10.48 -15.12 -5.08
Cytoplasmic Foci -1.50 14.44 10.20 10.07 -1.02 11.15 28.24 31.26 22.76 -1.56 5.33 29.34 31.04 25.18 -1.62
Eisosomes 11.82 10.35 8.63 -2.48 -1.22 2.97 3.61 5.00 8.39 4.45 10.59 10.62 13.20 6.35 4.60
Endoplasmic Reticulum 5.18 -6.26 -5.96 -7.18 -1.88 3.61 -5.06 -4.18 -8.09 3.29 5.63 -7.45 -5.63 -9.97 3.33
Endosome -10.98 4.86 3.81 11.97 -0.23 -12.17 6.33 5.48 16.36 -1.45 -18.86 7.04 4.77 19.52 -1.71
Golgi 0.15 26.05 24.05 13.09 -1.22 -11.03 13.21 11.44 20.71 -2.03 -4.92 28.17 24.99 24.17 -2.45
Lipid Particles 8.49 6.61 7.92 -0.31 1.17 9.93 5.73 14.95 10.93 3.66 13.92 8.17 20.35 8.69 4.97
Mitochondria 16.11 44.11* 44.27* 13.78 1.85 -12.99 18.40 17.31 31.84* -2.51 6.38 44.50* 43.17* 34.28* -2.24
Mitotic Spindle -5.05 -1.09 -2.27 1.19 -1.63 -4.27 -0.53 -4.87 -3.35 -3.70 -7.91 -1.11 -5.58 -3.14 -4.38
None 2.05 -0.17 4.90 2.49 4.12 3.05 -1.18 2.74 -0.85 2.99 4.09 -1.45 4.08 -0.06 3.67
Nuclear Periphery 1.99 -6.10 -5.05 -6.21 2.05 1.99 -2.20 -1.19 -4.97 1.70 1.83 -4.89 -4.41 -6.55 2.61
Nuclear Periphery Foci -0.99 -3.20 -4.87 -4.78 -1.33 0.55 -3.02 -5.43 -5.47 1.80 -0.42 -4.32 -7.55 -7.17 1.61
Nucleolus -1.83 -3.17 2.01 3.05 4.59 0.16 -1.98 1.92 1.44 2.50 -1.11 -3.05 1.71 2.61 3.71
Nucleus 0.48 -17.30 -7.55 -7.55 9.26 13.42 -20.66 -8.96 -15.27 15.42 9.65 -27.67 -14.05 -16.99 18.45
Peroxisomes -7.84 7.31 1.24 5.69 -1.66 -2.20 14.86 6.81 8.71 -4.28 -6.56 16.49 7.16 11.01 -3.61
Vacuole -7.76 -7.80 -13.31 -11.96 -8.41 -10.03 -7.09 -21.58 -18.32 -11.52 -15.52 -10.90 -25.85 -21.64 -14.03
Vacuole Periphery -0.45 13.61 14.90 6.86 2.60 -7.73 5.56 0.57 3.92 -1.94 -5.84 11.02 2.12 4.62 -2.03
Standard name
Human Ortholog
Description Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (49%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ecm18

Ecm18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available