Standard name
Human Ortholog
Description Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0 0.07 0.08 0.07 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 1.0 1.0 0.97 0.98 0.91 0.86 0.85 0.83 0.74 0.75 1.0 1.0 1.0 0.99 0.97 0.99 0.95 0.94 0.95 0.9 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.1 0.08 0.1 0.14 0.08 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 5 8 9 4 17 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 1 0 0 2 0 1 1 1 2 0 0 1 0 1 0 0 1 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 2 4 1 10 10 24 19 20 16 21 0 2 3 1 4 1 1 0 0 0 0 0
Cytoplasm 316 128 153 159 194 166 217 288 212 227 124 171 95 202 218 129 90 121 305 131 146 84 141 164
Endoplasmic Reticulum 5 4 0 1 7 3 18 34 21 26 24 19 2 1 1 5 6 9 6 0 3 4 6 5
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 2 0 0 0 0 1 0 5 7 12 11 26 0 0 0 1 0 0 0 0 0 1 0 3
Nucleus 0 0 0 0 0 2 0 1 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 1 0 1 2 2 4 0 0 0 0 0 1 0 3 0 1 1 2
Unique Cell Count 321 133 153 159 201 170 239 335 248 272 168 227 95 203 219 130 93 122 320 140 153 94 157 185
Labelled Cell Count 327 134 155 165 203 182 252 357 271 299 184 263 97 205 223 136 101 132 320 140 153 94 157 185


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 81.2 60.9 43.6 44.5 41.5 35.1 31.6 29.1 27.8 25.8 25.0 24.2 65.3 60.3 54.3 67.3 81.8 82.5 65.1 63.6 69.6
Std Deviation (1e-4) 18.3 13.4 9.2 12.4 10.8 8.0 7.4 7.4 6.9 6.5 6.9 6.7 13.6 11.1 9.3 20.1 23.7 18.5 16.2 17.9 20.9
Intensity Change (Log2) – – – 0.03 -0.07 -0.31 -0.46 -0.58 -0.65 -0.76 -0.8 -0.85 0.58 0.47 0.32 0.63 0.91 0.92 0.58 0.54 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.2 0 2.7 2.8 2.7 3.2 3.2 0 0 0 0 0 0
Cytoplasm 0 -2.3 -1.9 -3.9 -4.9 -4.9 -5.3 -6.8 -6.7 0 -0.9 -0.8 -1.1 -2.2 -1.1
Endoplasmic Reticulum 0 0 0 3.5 4.1 3.7 3.9 4.9 3.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.2 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 57.0218 83.9 74.2645 78.2528 71.3698 74.5059 72.8912 97.9468 90.3695 85.0061 79.5683 91.6436 71.5368 97.9165 88.2833 82.7394 76.262 89.2349
Actin 0.0219 0.0005 0.0012 0.0001 0.0002 0.0016 0.0063 0.0001 0.0002 0.003 0.0006 0.0004 0.0129 0.0007 0.0042 0.0152 0.0776 0.0025
Bud 0.003 0.0006 0.0019 0.0008 0.0012 0.0054 0.0014 0.0002 0.0004 0.0006 0.0009 0.0012 0.0016 0.0002 0.0008 0.0042 0.0014 0.0015
Bud Neck 0.0012 0.0007 0.0009 0.0011 0.0028 0.0061 0.0004 0.0003 0.0005 0.0009 0.0005 0.0008 0.0009 0.0002 0.0007 0.0016 0.0028 0.0009
Bud Periphery 0.0031 0.0004 0.0016 0.0003 0.0007 0.0073 0.0011 0.0001 0.0005 0.0005 0.0009 0.0012 0.0012 0.0001 0.0005 0.0017 0.001 0.001
Bud Site 0.009 0.0008 0.0017 0.0001 0.0002 0.0007 0.0013 0.0004 0.0003 0.0007 0.0003 0.0002 0.0011 0.0003 0.0038 0.0102 0.0147 0.0001
Cell Periphery 0.0134 0.0215 0.0053 0.0017 0.0053 0.0131 0.0093 0.0072 0.0075 0.0064 0.006 0.0068 0.004 0.0009 0.0021 0.0014 0.001 0.0011
Cytoplasm 0.5778 0.881 0.8045 0.7129 0.8087 0.7164 0.8224 0.9665 0.9065 0.8202 0.8802 0.9156 0.8121 0.9835 0.9187 0.8464 0.8136 0.8939
Cytoplasmic Foci 0.0078 0.0006 0.0089 0.0007 0.0016 0.0025 0.0121 0.0005 0.0082 0.0026 0.0073 0.003 0.0114 0.0008 0.0018 0.0035 0.0114 0.0049
Eisosomes 0.0002 0 0 0 0 0 0.0004 0 0 0.001 0.0001 0 0.0004 0 0.0001 0.0002 0.0003 0
Endoplasmic Reticulum 0.0556 0.035 0.0239 0.0018 0.0082 0.0314 0.0251 0.0043 0.0117 0.0068 0.0033 0.0043 0.0248 0.0028 0.0145 0.0013 0.0044 0.0332
Endosome 0.0109 0.0012 0.0098 0.0032 0.0068 0.0093 0.0067 0.0007 0.0023 0.0043 0.0054 0.0066 0.0131 0.0009 0.0034 0.0057 0.0204 0.0143
Golgi 0.0024 0.0002 0.0016 0.0001 0.0002 0.0009 0.0028 0.0001 0.0003 0.0008 0.0005 0.0006 0.0041 0.0004 0.0004 0.0008 0.0296 0.0022
Lipid Particles 0.0041 0.002 0.0022 0.0001 0.0005 0.0011 0.0114 0.0006 0.0021 0.0051 0.0029 0.0003 0.0046 0.0001 0.0009 0.001 0.0022 0.0009
Mitochondria 0.0103 0.0002 0.004 0.0001 0.0003 0.0026 0.0068 0 0.0005 0.0149 0.01 0.0037 0.0064 0.0001 0.004 0.0006 0.0034 0.0015
None 0.0045 0.0002 0.0008 0.0001 0.0002 0.0004 0.0155 0.0001 0.0013 0.0044 0.0022 0.0006 0.003 0.0001 0.0003 0.0008 0.0003 0.0008
Nuclear Periphery 0.0618 0.0051 0.0125 0.0079 0.0206 0.025 0.0105 0.0011 0.0027 0.0083 0.0232 0.002 0.0122 0.001 0.0036 0.0046 0.0011 0.0192
Nucleolus 0.0084 0.0001 0.0006 0.0001 0.0002 0.0006 0.0055 0 0.0004 0.0012 0.0007 0.0006 0.0016 0 0.0002 0.0005 0.0001 0.0003
Nucleus 0.024 0.0027 0.005 0.0045 0.0057 0.0103 0.0077 0.0009 0.0021 0.0034 0.0125 0.0021 0.0046 0.0008 0.0018 0.0038 0.0012 0.0029
Peroxisomes 0.0017 0 0.0004 0 0.0001 0.0001 0.0033 0 0.0002 0.0002 0.0003 0.0001 0.003 0 0.0003 0.001 0.0008 0.0001
Punctate Nuclear 0.0035 0 0.0002 0 0.0001 0.0002 0.0012 0 0.0065 0.0004 0.0003 0 0.0013 0 0.0001 0.0009 0.0002 0.0023
Vacuole 0.1449 0.0444 0.0921 0.2275 0.1126 0.1383 0.0422 0.0163 0.0426 0.1053 0.0301 0.0378 0.0611 0.0066 0.0316 0.0779 0.0087 0.0112
Vacuole Periphery 0.0304 0.0028 0.0208 0.0371 0.0237 0.0268 0.0066 0.0007 0.0032 0.0089 0.0115 0.0123 0.0145 0.0005 0.0063 0.0165 0.0039 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 128.5801 123.3229 122.8334 133.0275 142.551 130.2439 146.2407 146.2889 148.4481 160.3466
Translational Efficiency 1.1483 1.1334 1.0223 0.997 0.9173 1.0071 0.9398 0.7542 0.778 0.7877

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
93 655 697 1332 1757 1892 243 321 1850 2547 940 1653

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2359.21 4684.88 5260.36 2516.35 2967.40 4101.32 3935.73 2971.03 2936.83 4251.39 4917.93 2604.65
Standard Deviation 787.92 930.80 968.77 562.84 812.19 935.23 624.32 572.59 821.80 968.29 1064.42 592.70
Intensity Change Log 2 — 0.989708 1.156858 0.093029 — 0.466889 0.407432 0.001764 — 0.722022 0.787803 0.042900

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000954 0.000092 0.000205 0.001263 0.000056 0.000080 0.000291 0.000439 0.000101 0.000083 0.000227 0.001103
Bud Neck 0.001851 0.000896 0.003743 0.001628 0.000171 0.000472 0.005079 0.001959 0.000256 0.000581 0.004088 0.001692
Bud Site 0.042300 0.003733 0.004404 0.011713 0.002021 0.002629 0.011177 0.021243 0.004046 0.002913 0.006155 0.013564
Cell Periphery 0.043872 0.013624 0.028287 0.002473 0.003515 0.003380 0.025071 0.017150 0.005544 0.006014 0.027456 0.005323
Cytoplasm 0.453385* 0.776597* 0.649210* 0.807715* 0.888591* 0.886423* 0.626615* 0.801883* 0.866713* 0.858180* 0.643369* 0.806582*
Cytoplasmic Foci 0.046794 0.012870 0.020507 0.010451 0.004647 0.009075 0.029872 0.006972 0.006766 0.010051 0.022928 0.009775
Eisosomes 0.000012 0.000002 0.000005 0.000005 0.000001 0.000001 0.000005 0.000004 0.000001 0.000001 0.000005 0.000005
Endoplasmic Reticulum 0.007441 0.000434 0.000228 0.000282 0.000314 0.000206 0.000298 0.000203 0.000672 0.000265 0.000246 0.000266
Endosome 0.008636 0.000742 0.000712 0.003549 0.000344 0.000654 0.001806 0.000609 0.000760 0.000677 0.000995 0.002978
Golgi 0.157014* 0.057945 0.039173 0.009813 0.020992 0.026771 0.038134 0.011860 0.027830 0.034788 0.038904 0.010211
Lipid Particles 0.018980 0.012287 0.018121 0.006895 0.004092 0.004387 0.033380 0.020814 0.004840 0.006419 0.022065 0.009598
Mitochondria 0.000300 0.000075 0.000191 0.000683 0.000166 0.000144 0.000071 0.000060 0.000173 0.000126 0.000160 0.000562
Mitotic Spindle 0.003097 0.001922 0.003459 0.008803 0.000269 0.001870 0.005106 0.000624 0.000411 0.001883 0.003884 0.007215
None 0.000772 0.002033 0.002614 0.000854 0.003465 0.001960 0.001257 0.000488 0.003329 0.001979 0.002263 0.000783
Nuclear Periphery 0.000493 0.000534 0.002420 0.000142 0.000114 0.000227 0.001111 0.000103 0.000134 0.000306 0.002082 0.000134
Nuclear Periphery Foci 0.008637 0.015052 0.015410 0.002928 0.005048 0.007997 0.016983 0.003792 0.005229 0.009811 0.015817 0.003096
Nucleolus 0.002300 0.000052 0.000119 0.000189 0.000077 0.000128 0.000100 0.000093 0.000189 0.000109 0.000114 0.000170
Nucleus 0.007847 0.014368 0.054719 0.014829 0.010474 0.010379 0.028553 0.007289 0.010342 0.011405 0.047955 0.013365
Peroxisomes 0.000422 0.000013 0.000013 0.000406 0.000026 0.000031 0.000043 0.000148 0.000046 0.000027 0.000021 0.000356
Vacuole 0.062237 0.051755 0.117415 0.104218 0.030963 0.025690 0.152353 0.097789 0.032535 0.032393 0.126447 0.102970
Vacuole Periphery 0.132656* 0.034974 0.039046 0.011159 0.024654 0.017495 0.022693 0.006477 0.030083 0.021990 0.034818 0.010250

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.43 2.82 -0.27 -1.95 -1.66 -1.10 -7.22 -3.84 -3.56 -1.09 0.59 -6.51 -2.10 -2.13 -1.70
Bud Neck 2.22 -5.63 -1.14 -5.92 5.94 -5.56 -8.02 -5.07 -4.18 4.80 -6.01 -13.97 -13.06 -10.04 7.66
Bud Site 4.63 4.52 3.57 -8.90 -7.77 -1.74 -3.64 -4.41 -4.25 -1.81 1.84 -2.69 -8.29 -9.97 -5.64
Cell Periphery 3.25 1.55 4.57 8.37 13.96 0.21 -6.67 -6.73 -6.82 2.07 -0.76 -12.86 0.87 1.98 13.66
Cytoplasm -8.12 -4.20 -8.66 -0.91 -11.50 0.73 13.88 7.16 6.79 -7.86 1.67 21.18 10.05 9.03 -13.85
Cytoplasmic Foci 5.00 3.37 5.37 1.72 6.90 -6.45 -8.61 -3.60 1.54 7.47 -4.82 -12.07 -4.57 -0.02 9.62
Eisosomes 3.11 1.51 2.07 -2.53 1.68 -1.11 -7.00 -5.44 -4.80 2.50 0.48 -10.74 -3.36 -3.51 2.07
Endoplasmic Reticulum 4.96 5.11 5.09 1.57 -0.80 2.18 0.68 1.53 0.19 1.09 4.32 4.77 4.53 0.35 -0.20
Endosome 4.93 4.92 3.20 -4.65 -4.63 -2.38 -7.86 -3.18 0.15 5.77 0.45 -2.68 -4.52 -4.65 -3.85
Golgi 5.53 6.74 8.50 13.23 13.17 -2.83 -4.24 4.83 7.14 6.95 -3.13 -4.05 10.12 15.18 14.97
Lipid Particles 1.26 -0.32 2.40 3.78 8.34 -0.62 -9.03 -6.67 -6.56 3.23 -2.49 -13.04 -5.63 -3.98 9.01
Mitochondria 2.06 0.76 -1.01 -2.08 -1.47 0.33 3.04 3.20 1.97 0.93 0.98 -0.29 -1.53 -1.75 -1.50
Mitotic Spindle 1.85 -1.28 -3.08 -3.81 -2.62 -4.34 -5.46 -2.16 3.04 4.99 -5.20 -9.50 -4.76 -3.59 -1.86
None -5.08 -9.56 -0.07 4.51 8.01 3.06 5.04 6.74 6.15 4.66 3.00 2.60 5.90 5.45 8.28
Nuclear Periphery -0.24 -6.54 1.13 7.34 12.17 -4.32 -7.55 0.46 4.79 7.62 -6.32 -13.53 -0.84 5.59 13.40
Nuclear Periphery Foci -2.45 -2.94 2.23 9.30 11.04 -4.01 -7.72 1.99 5.68 8.52 -6.58 -10.94 3.97 10.55 13.58
Nucleolus 1.00 0.97 0.93 -8.64 -4.09 -1.34 -3.15 -1.46 1.03 1.75 0.61 0.56 0.03 -1.84 -3.92
Nucleus -1.05 -9.21 -2.17 -2.00 10.97 -0.48 -6.92 1.24 1.79 7.40 -1.35 -14.58 -5.16 -4.36 12.08
Peroxisomes 2.09 2.08 0.28 -2.27 -2.26 -0.64 -2.61 -4.30 -3.75 -3.54 1.17 1.95 -2.21 -2.35 -2.40
Vacuole 0.65 -5.37 -5.20 -10.19 0.76 1.54 -10.78 -8.64 -9.19 3.96 -0.45 -16.71 -17.81 -18.00 2.57
Vacuole Periphery 4.58 4.47 5.84 8.08 8.66 3.31 1.25 9.83 7.16 4.65 3.41 -0.92 9.91 8.80 9.51
Standard name
Human Ortholog
Description Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Aro1

Aro1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available