Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.99 0.91 0.86 0.88 0.83 – 0.75 0.78 0.7 0.73 0.98 0.99 0.97 0.95 0.96 0.97 0.93 0.93 0.97 0.93 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.18 0.05 – 0.26 0.2 0.34 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.13 0.19 0.08 0.21 – 0.13 0.11 0.1 0.07 0 0 0.06 0.05 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 1 1 0 3 1
Bud 0 0 0 1 0 5 2 – 5 3 7 5 0 0 0 0 0 0 1 0 2 1 4 9
Bud Neck 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 5 1 5 – 9 10 1 3 0 0 2 9 10 11 0 0 0 0 0 0
Cytoplasm 460 486 568 488 473 465 694 – 574 633 268 334 494 600 724 282 288 266 442 474 546 337 526 597
Endoplasmic Reticulum 0 3 1 0 1 0 0 – 0 1 0 1 4 1 1 9 8 12 0 0 2 4 4 5
Endosome 1 1 1 2 1 0 3 – 2 4 1 2 0 0 3 2 0 0 5 4 0 3 1 2
Golgi 2 1 1 1 0 0 0 – 0 0 0 0 2 1 0 0 0 0 5 2 0 2 1 3
Mitochondria 1 7 0 11 16 94 45 – 197 159 132 134 0 1 0 0 3 1 4 8 2 0 4 4
Nucleus 1 4 3 3 5 6 17 – 15 16 12 11 1 0 0 6 3 3 2 3 0 0 1 2
Nuclear Periphery 1 0 0 1 1 0 0 – 4 3 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 – 2 1 1 0 0 0 0 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 4 – 1 1 1 0 0 0 0 0 0 0 0 1 0 2 3 3
Vac/Vac Membrane 10 5 17 71 102 40 174 – 97 89 37 30 18 16 41 15 21 13 0 5 0 3 3 2
Unique Cell Count 472 499 574 536 548 531 832 769 811 385 460 505 609 744 296 301 275 473 509 563 363 558 648
Labelled Cell Count 476 508 591 579 608 611 944 907 920 460 522 519 619 771 324 333 306 473 509 563 363 558 648


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.5 7.4 6.9 7.3 5.2 6.1 – 5.1 5.4 5.1 5.2 7.8 7.8 7.9 8.0 8.6 7.6 6.8 7.1 7.5
Std Deviation (1e-4) 0.9 0.9 1.1 1.3 1.5 1.3 1.3 – 1.1 1.0 1.0 1.1 1.2 1.2 1.4 1.1 1.4 1.2 1.3 1.6 1.6
Intensity Change (Log2) – – – -0.11 -0.03 -0.52 -0.29 – -0.56 -0.47 -0.54 -0.52 0.07 0.07 0.09 0.11 0.2 0.02 -0.14 -0.07 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.1 -8.2 -7.7 -9.4 – -12.4 -11.3 -13.3 -12.6 -1.5 -0.7 -2.1 -3.4 -3.2 -2.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.4 4.1 10.5 5.7 – 13.1 11.3 15.1 13.9 0 0 0 0 0 0
Nucleus 0 0 0 2.4 – 2.3 2.3 3.2 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 6.3 8.5 3.4 9.7 – 6.3 5.5 4.4 2.7 0.6 -0.3 2.2 1.6 2.8 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1095 2.204 1.603 1.3634 1.3036 1.6782 3.7604 3.5685 3.5009 3.1483 2.6789 3.5743 5.2814 6.903 6.1165 6.0966 6.5024 6.2553
Actin 0.0081 0.0017 0.004 0.0033 0.0011 0.002 0.0593 0.0069 0.0123 0.0345 0.0001 0.0113 0.0022 0.0003 0.0013 0.0005 0.0036 0.0005
Bud 0.0053 0.0037 0.0107 0.0099 0.0004 0.0027 0.0018 0.0007 0.0028 0.0019 0.0001 0.0009 0.0002 0.0001 0.0005 0.0002 0.0028 0.0001
Bud Neck 0.0004 0.0005 0.0007 0.0006 0.0006 0.0023 0.0099 0.0004 0.005 0.0005 0.0002 0.0026 0.0008 0.0002 0.0003 0.0006 0.0162 0.0006
Bud Periphery 0.0022 0.001 0.0052 0.0098 0.0005 0.0021 0.0027 0.0003 0.0031 0.0024 0 0.0017 0.0001 0 0.0007 0.0001 0.0016 0
Bud Site 0.0049 0.0145 0.0063 0.0015 0.0002 0.0004 0.0141 0.006 0.014 0.001 0 0.0019 0.0009 0.0006 0.0026 0.0002 0.0025 0
Cell Periphery 0.0002 0.0002 0.0002 0.0004 0 0.0001 0.0007 0.0001 0.0004 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.7491 0.8185 0.7877 0.8038 0.8886 0.8342 0.5799 0.8457 0.831 0.6506 0.8905 0.8034 0.809 0.89 0.849 0.8994 0.7638 0.8843
Cytoplasmic Foci 0.0171 0.0163 0.0118 0.0159 0.0107 0.0146 0.0198 0.0057 0.0148 0.0372 0.0043 0.0134 0.0049 0.0029 0.0037 0.005 0.0087 0.0047
Eisosomes 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0003 0 0.0002 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.004 0.003 0.0036 0.0045 0.0032 0.0025 0.0198 0.0022 0.0028 0.0025 0.002 0.0018 0.0044 0.0015 0.0022 0.0044 0.0028 0.0019
Endosome 0.0069 0.0046 0.009 0.0104 0.005 0.0092 0.0312 0.0016 0.0122 0.0104 0.0018 0.0066 0.0041 0.0007 0.0016 0.0027 0.0016 0.0012
Golgi 0.0006 0.0002 0.0003 0.0002 0.0002 0.0002 0.0099 0.0003 0.0024 0.0097 0.0001 0.0027 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001
Lipid Particles 0.0006 0.0002 0.0003 0.0002 0.0001 0.0002 0.0112 0.0002 0.0009 0.0179 0 0.0031 0.0008 0 0.0001 0.0001 0.0001 0
Mitochondria 0.0008 0.0004 0.0008 0.0008 0.0003 0.0004 0.0067 0.0002 0.0011 0.0446 0.0001 0.0023 0.0003 0.0001 0.0003 0.0002 0.0002 0.0001
None 0.1752 0.0832 0.1115 0.0844 0.0642 0.0771 0.1413 0.1209 0.0735 0.0755 0.0894 0.1243 0.155 0.0968 0.1295 0.0732 0.1853 0.0996
Nuclear Periphery 0.003 0.0045 0.0049 0.0047 0.0025 0.0032 0.0492 0.0011 0.0019 0.005 0.0012 0.0045 0.0029 0.0007 0.0011 0.0018 0.0011 0.0006
Nucleolus 0.0005 0.0006 0.0006 0.0018 0.0001 0.0005 0.0007 0.0001 0.0009 0.0002 0 0.0002 0.0006 0 0.0001 0 0.0002 0
Nucleus 0.0135 0.0288 0.028 0.0354 0.0168 0.0339 0.0112 0.0045 0.0109 0.0089 0.0091 0.0123 0.0047 0.0047 0.0052 0.0094 0.0051 0.005
Peroxisomes 0.0005 0.0003 0.0005 0.0002 0.0005 0.0002 0.0073 0.0006 0.0031 0.0922 0 0.0021 0.0004 0 0.0001 0.0001 0.0002 0.0001
Punctate Nuclear 0.0043 0.0154 0.0113 0.0088 0.0039 0.0123 0.0115 0.0017 0.0052 0.0024 0.0004 0.0031 0.0071 0.0006 0.0007 0.0009 0.0032 0.0008
Vacuole 0.0023 0.0021 0.0022 0.003 0.001 0.0018 0.006 0.0009 0.0013 0.0013 0.0004 0.0012 0.0008 0.0005 0.0007 0.0009 0.0005 0.0003
Vacuole Periphery 0.0003 0.0003 0.0004 0.0005 0.0002 0.0003 0.0052 0.0001 0.0003 0.0011 0.0001 0.0004 0.0002 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.4785 42.9503 29.673 28.6528 22.5547 13.2468 29.9497 29.3103 31.1271 28.01
Translational Efficiency 1.0157 0.514 0.7425 0.7929 1.0202 1.9313 0.9231 0.7772 0.8935 0.8525

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
349 1640 165 2339 2105 1731 2096 1500 2454 3371 2261 3839

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 721.03 913.54 1016.14 976.70 770.66 913.43 979.70 1065.39 763.60 913.48 982.36 1011.35
Standard Deviation 100.20 132.13 110.87 149.32 101.18 109.80 127.01 153.36 102.51 121.18 126.26 157.00
Intensity Change Log 2 — 0.341409 0.494968 0.437856 — 0.245200 0.346246 0.467215 — 0.292505 0.420048 0.453099

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000618 0.000246 0.000667 0.000330 0.000098 0.000113 0.000198 0.000677 0.000172 0.000177 0.000232 0.000465
Bud Neck 0.008377 0.002439 0.002153 0.006142 0.004887 0.006439 0.003134 0.002106 0.005384 0.004493 0.003063 0.004565
Bud Site 0.023254 0.006049 0.005200 0.020307 0.004223 0.010272 0.021484 0.014599 0.006929 0.008217 0.020295 0.018077
Cell Periphery 0.000460 0.000107 0.000320 0.000079 0.000085 0.000055 0.000077 0.000092 0.000138 0.000080 0.000095 0.000084
Cytoplasm 0.532330* 0.730799* 0.430902* 0.723234* 0.666981* 0.725242* 0.730886* 0.647873* 0.647831* 0.727946* 0.708994* 0.693788*
Cytoplasmic Foci 0.187127* 0.079911 0.028280 0.007417 0.113235 0.055055 0.007668 0.007799 0.123743 0.067147 0.009172 0.007566
Eisosomes 0.000109 0.000024 0.000101 0.000010 0.000043 0.000021 0.000009 0.000014 0.000053 0.000022 0.000016 0.000012
Endoplasmic Reticulum 0.006836 0.001669 0.007250 0.000608 0.000983 0.000364 0.000723 0.001100 0.001816 0.000999 0.001199 0.000800
Endosome 0.005692 0.004290 0.010266 0.001315 0.003943 0.002773 0.000840 0.002137 0.004192 0.003511 0.001528 0.001636
Golgi 0.001716 0.000782 0.000200 0.000697 0.000926 0.000690 0.000395 0.003619 0.001038 0.000735 0.000381 0.001839
Lipid Particles 0.003806 0.001293 0.004068 0.000339 0.002005 0.000529 0.000266 0.000526 0.002261 0.000901 0.000544 0.000412
Mitochondria 0.004512 0.000323 0.000223 0.000572 0.001065 0.000961 0.000528 0.000407 0.001555 0.000651 0.000506 0.000508
Mitotic Spindle 0.002128 0.001794 0.000634 0.018649 0.003078 0.007776 0.018052 0.023106 0.002943 0.004866 0.016781 0.020390
None 0.004540 0.006388 0.002353 0.008974 0.004850 0.004444 0.005824 0.012469 0.004806 0.005390 0.005571 0.010339
Nuclear Periphery 0.000534 0.000210 0.001582 0.000517 0.000316 0.000235 0.000532 0.000599 0.000347 0.000223 0.000609 0.000549
Nuclear Periphery Foci 0.001747 0.002278 0.011979 0.000930 0.000693 0.000261 0.000757 0.001549 0.000843 0.001243 0.001576 0.001172
Nucleolus 0.000694 0.000376 0.000369 0.000274 0.000610 0.000497 0.000332 0.000136 0.000622 0.000438 0.000334 0.000220
Nucleus 0.165860* 0.119912 0.253085* 0.160421* 0.154439* 0.138612* 0.157034* 0.230893* 0.156063* 0.129515 0.164044* 0.187956*
Peroxisomes 0.003077 0.001038 0.000381 0.000183 0.001329 0.000996 0.000222 0.000344 0.001577 0.001016 0.000233 0.000246
Vacuole 0.044825 0.039690 0.239713* 0.048507 0.035379 0.044084 0.050851 0.049362 0.036722 0.041946 0.064634 0.048841
Vacuole Periphery 0.001757 0.000381 0.000274 0.000497 0.000832 0.000582 0.000188 0.000591 0.000963 0.000484 0.000195 0.000534

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.01 -1.82 2.41 -0.82 3.81 -0.84 -3.05 -3.70 -3.49 -3.10 -0.42 -1.91 -3.20 -3.06 -2.56
Bud Neck 4.63 4.26 2.39 -3.48 -2.85 -1.81 2.59 2.60 4.90 0.15 1.33 3.74 1.59 0.42 -2.35
Bud Site 4.89 4.67 3.39 -3.60 -2.99 -4.13 -5.64 -3.40 0.87 2.52 -1.25 -3.83 -2.82 -1.64 1.80
Cell Periphery 4.71 2.12 5.20 5.63 8.24 7.15 2.97 -1.88 -9.57 -4.49 4.93 4.40 5.07 0.23 0.72
Cytoplasm -13.29 7.81 -4.16 18.11 -12.22 -7.58 3.35 19.90 26.07 16.25 -12.65 3.72 14.07 29.81 9.01
Cytoplasmic Foci 9.84 15.63 17.45 24.58 5.44 15.05 33.50 33.63 20.85 0.92 15.51 36.80 37.66 32.09 3.01
Eisosomes 9.80 -0.08 10.62 4.26 12.14 10.19 17.05 10.78 -0.63 -9.87 13.84 15.03 15.70 3.16 0.82
Endoplasmic Reticulum 5.51 -1.48 6.63 3.69 9.52 3.76 1.04 -2.46 -11.08 -4.11 3.63 1.98 3.95 -0.32 1.96
Endosome 1.54 -2.89 6.53 6.42 8.47 2.84 10.81 8.06 4.75 -1.39 1.68 9.82 11.10 8.15 2.69
Golgi 2.99 5.00 4.42 3.31 -0.75 0.92 3.33 -0.00 -0.45 -1.33 1.60 4.28 1.84 0.74 -1.36
Lipid Particles 4.73 -0.04 6.18 5.10 8.86 8.16 9.09 5.00 -5.08 -7.08 7.58 9.33 8.77 1.53 -1.84
Mitochondria 2.52 2.55 2.40 -3.61 -6.43 0.44 2.72 3.00 2.68 0.82 3.01 3.58 3.63 1.49 0.13
Mitotic Spindle 0.08 -0.06 -6.98 -8.02 -7.44 -2.47 -6.91 -5.46 -2.89 1.06 -1.65 -6.99 -8.72 -7.15 0.23
None -1.15 1.85 -2.64 -2.67 -14.22 0.62 -1.36 -5.98 -6.61 -5.89 -0.71 -1.09 -6.71 -6.83 -7.89
Nuclear Periphery 1.62 -6.58 -3.35 -23.72 6.49 1.07 -12.38 -16.68 -16.43 -5.77 2.13 -12.82 -16.83 -25.78 -3.38
Nuclear Periphery Foci -1.34 -7.20 1.90 5.81 8.06 4.66 -0.14 -7.20 -12.28 -4.94 -3.31 -3.07 -4.23 -0.25 0.78
Nucleolus 2.78 3.55 4.46 1.67 0.66 0.85 2.01 3.12 3.18 1.59 1.49 2.35 3.23 3.35 0.92
Nucleus 4.82 -5.30 -5.07 -20.48 2.99 3.22 -8.93 -22.51 -24.58 -14.52 6.77 -9.87 -20.86 -30.42 -8.83
Peroxisomes 4.89 7.21 7.89 5.54 5.62 0.75 3.80 3.64 4.98 -0.40 1.73 5.17 5.28 7.58 0.53
Vacuole 0.95 -12.75 -14.07 -20.32 8.64 -4.21 -17.12 -20.64 -17.73 -6.51 -3.57 -19.66 -29.15 -26.82 -3.86
Vacuole Periphery 2.96 3.20 3.25 1.74 0.55 0.92 2.73 2.34 2.41 -0.54 2.04 3.57 3.16 2.94 -1.09
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mtq2

Mtq2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtq2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available