Standard name | |
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Human Ortholog | |
Description | Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 15 | 7 | 6 | 7 | 8 | 7 | 11 | 12 | 2 | 0 | 8 | 25 | 21 | 17 | 35 | 23 | 5 | 20 | 13 | 20 | 9 | 9 | 11 |
Bud | 2 | 1 | 4 | 5 | 12 | 29 | 42 | 29 | 33 | 28 | 22 | 15 | 2 | 2 | 4 | 2 | 3 | 2 | 1 | 0 | 1 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 1 | 3 | 4 | 11 | 14 | 11 | 18 | 9 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 1 | 0 | 3 | 3 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 1 | 7 | 12 | 10 | 10 | 16 | 17 | 9 | 16 | 14 | 12 | 4 | 17 | 13 | 32 | 12 | 11 | 0 | 0 | 2 | 2 | 2 | 3 |
Endoplasmic Reticulum | 2 | 3 | 1 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | 0 | 1 | 4 | 0 | 1 | 4 | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Endosome | 11 | 3 | 19 | 23 | 17 | 17 | 12 | 29 | 15 | 11 | 14 | 4 | 12 | 18 | 22 | 4 | 11 | 5 | 39 | 14 | 31 | 24 | 18 | 23 |
Golgi | 58 | 35 | 62 | 18 | 14 | 5 | 1 | 2 | 7 | 2 | 1 | 3 | 175 | 213 | 158 | 137 | 101 | 82 | 152 | 73 | 158 | 127 | 140 | 194 |
Mitochondria | 192 | 85 | 160 | 155 | 127 | 238 | 279 | 322 | 283 | 269 | 183 | 207 | 78 | 33 | 51 | 13 | 12 | 12 | 10 | 7 | 11 | 7 | 8 | 12 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 3 | 2 | 3 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 0 | 3 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 31 | 15 | 26 | 19 | 13 | 25 | 39 | 60 | 37 | 45 | 34 | 37 | 14 | 16 | 13 | 10 | 16 | 13 | 1 | 2 | 2 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 23 | 33 | 17 | 41 | 50 | 44 | 28 | 36 | 26 | 10 | 4 | 1 | 3 | 2 | 0 | 0 | 17 | 2 | 31 | 8 | 5 | 9 |
SpindlePole | 1 | 0 | 7 | 11 | 5 | 14 | 19 | 29 | 25 | 25 | 19 | 13 | 6 | 15 | 15 | 5 | 3 | 3 | 4 | 2 | 8 | 2 | 1 | 0 |
Vac/Vac Membrane | 16 | 7 | 8 | 2 | 5 | 1 | 11 | 11 | 6 | 5 | 3 | 5 | 10 | 25 | 19 | 15 | 14 | 13 | 4 | 4 | 4 | 8 | 5 | 5 |
Unique Cell Count | 226 | 107 | 236 | 220 | 180 | 297 | 366 | 439 | 362 | 361 | 239 | 258 | 223 | 289 | 233 | 202 | 149 | 120 | 256 | 125 | 279 | 199 | 200 | 271 |
Labelled Cell Count | 334 | 169 | 330 | 286 | 231 | 399 | 496 | 573 | 474 | 465 | 327 | 330 | 336 | 362 | 318 | 261 | 202 | 147 | 256 | 125 | 279 | 199 | 200 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.3 | 15.0 | 13.0 | 11.8 | 11.0 | 9.1 | 8.4 | 8.2 | 7.9 | 7.6 | 7.1 | 7.0 | 14.8 | 14.7 | 13.6 | 18.4 | 16.4 | 16.9 | 15.6 | 15.7 | 17.1 |
Std Deviation (1e-4) | 2.4 | 3.0 | 2.3 | 2.1 | 2.2 | 1.9 | 2.0 | 1.7 | 1.6 | 1.7 | 1.9 | 1.6 | 2.5 | 2.8 | 2.5 | 5.4 | 4.1 | 3.9 | 3.4 | 3.1 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.24 | -0.52 | -0.63 | -0.67 | -0.72 | -0.78 | -0.87 | -0.89 | 0.19 | 0.18 | 0.06 | 0.5 | 0.33 | 0.38 | 0.26 | 0.27 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | 0.5 | -0.2 | -0.8 | -0.4 | 0.2 | 0 | 0 | 0.1 | 3.5 | 2.2 | 2.1 | 5.1 | 4.4 | 0 |
Bud | 0 | 2.6 | 3.8 | 4.4 | 2.8 | 3.7 | 3.2 | 3.6 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 1.3 | 0.3 | 0.9 | 0.6 | -0.4 | 0.9 | 1.5 | 1.0 | -0.8 | 1.6 | 1.4 | 4.7 | 2.2 | 2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.9 | 0.5 | -1.1 | -2.6 | -0.7 | -2.0 | -2.7 | -0.9 | -3.4 | -1.1 | -0.8 | 0.5 | -2.8 | -0.2 | -1.4 |
Golgi | -5.1 | -4.8 | -8.5 | -10.2 | -10.9 | -9.1 | -9.9 | -8.3 | -8.2 | 11.2 | 10.8 | 9.0 | 8.7 | 8.0 | 7.6 |
Mitochondria | 0.6 | 0.6 | 3.3 | 2.3 | 1.5 | 2.8 | 1.8 | 2.1 | 3.2 | -7.0 | -13.3 | -10.0 | -13.1 | -11.5 | -10.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.9 | -1.3 | -1.0 | -0.1 | 1.0 | -0.3 | 0.5 | 1.1 | 1.1 | -1.8 | -2.3 | -2.1 | -2.3 | -0.1 | -0.1 |
Peroxisomes | 1.7 | -0.1 | 1.4 | 1.4 | 0.1 | -0.9 | 0.1 | 0.4 | -2.6 | -3.6 | -5.1 | -4.0 | -3.9 | -3.9 | -3.5 |
SpindlePole | 1.1 | -0.1 | 1.0 | 1.3 | 2.0 | 2.1 | 2.1 | 2.4 | 1.2 | -0.2 | 1.3 | 1.8 | -0.3 | 0 | 0 |
Vacuole | 0 | -0.4 | 0 | -0.3 | -0.7 | -1.4 | -1.6 | -1.5 | -1.0 | 0.6 | 2.5 | 2.2 | 1.9 | 2.5 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.6659 | 10.4907 | 9.5248 | 9.0659 | 8.8749 | 10.1115 | 8.8819 | 11.8431 | 11.1741 | 10.8112 | 11.6822 | 10.9095 | 9.1757 | 11.5133 | 9.6588 | 9.1718 | 9.912 | 10.6174 |
Actin | 0.0675 | 0.0253 | 0.064 | 0.0072 | 0.0279 | 0.0461 | 0.0372 | 0.022 | 0.0272 | 0.0794 | 0.0002 | 0.0378 | 0.0523 | 0.0274 | 0.0257 | 0.0018 | 0.0123 | 0.0174 |
Bud | 0.0005 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0012 | 0.0007 | 0.007 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0004 |
Bud Neck | 0.0009 | 0.0004 | 0.0022 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0008 | 0.0011 | 0.0019 | 0.0001 | 0.0008 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0004 |
Bud Periphery | 0.0006 | 0.0001 | 0.0007 | 0.0006 | 0.0002 | 0.0003 | 0.001 | 0.001 | 0.0008 | 0.0066 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0004 |
Bud Site | 0.0011 | 0.0005 | 0.0027 | 0.0005 | 0.0006 | 0.0003 | 0.0015 | 0.0076 | 0.0019 | 0.0126 | 0 | 0.0002 | 0.0006 | 0.0012 | 0.0008 | 0.0001 | 0.0001 | 0.0004 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0044 | 0.001 | 0.0025 | 0.0006 | 0.0011 | 0.0005 | 0.0025 | 0.0019 | 0.0037 | 0.0024 | 0.0004 | 0.0008 | 0.003 | 0.0008 | 0.0008 | 0.0007 | 0.0004 | 0.0013 |
Cytoplasmic Foci | 0.0742 | 0.0811 | 0.0627 | 0.0457 | 0.062 | 0.0391 | 0.0864 | 0.0704 | 0.0653 | 0.0787 | 0.0366 | 0.0514 | 0.0865 | 0.075 | 0.0765 | 0.0521 | 0.0627 | 0.0552 |
Eisosomes | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0023 | 0.0002 | 0.0007 | 0.0004 | 0.0008 | 0.0003 | 0.0032 | 0.0004 | 0.001 | 0.0006 | 0.0002 | 0.0003 | 0.0021 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0006 |
Endosome | 0.1575 | 0.138 | 0.1405 | 0.269 | 0.159 | 0.1 | 0.174 | 0.197 | 0.1766 | 0.3213 | 0.2518 | 0.1418 | 0.151 | 0.1506 | 0.1447 | 0.2377 | 0.1238 | 0.123 |
Golgi | 0.5623 | 0.7161 | 0.6016 | 0.5573 | 0.6251 | 0.7117 | 0.5157 | 0.6205 | 0.5866 | 0.3791 | 0.683 | 0.7332 | 0.5737 | 0.6796 | 0.6763 | 0.6326 | 0.6579 | 0.7089 |
Lipid Particles | 0.0299 | 0.0046 | 0.0172 | 0.0186 | 0.0403 | 0.0191 | 0.0468 | 0.0281 | 0.0303 | 0.0577 | 0.0172 | 0.0108 | 0.0196 | 0.0173 | 0.0184 | 0.0498 | 0.1163 | 0.0229 |
Mitochondria | 0.0542 | 0.0085 | 0.0497 | 0.0445 | 0.0461 | 0.0459 | 0.0933 | 0.022 | 0.0548 | 0.0147 | 0.0025 | 0.0104 | 0.036 | 0.028 | 0.023 | 0.0104 | 0.005 | 0.0357 |
None | 0.0029 | 0 | 0.0036 | 0 | 0.0006 | 0.0001 | 0.0013 | 0.0001 | 0.0006 | 0.0002 | 0 | 0 | 0.0075 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0049 | 0 | 0.0012 | 0.0002 | 0.0007 | 0.01 | 0.0054 | 0.0003 | 0.0052 | 0.0008 | 0.0001 | 0.0002 | 0.0255 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0036 |
Nucleolus | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0016 | 0.0054 | 0.0008 | 0.0002 | 0 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.003 | 0 | 0.0012 | 0.0001 | 0.0002 | 0.0002 | 0.0016 | 0.0006 | 0.0055 | 0.0003 | 0 | 0.0001 | 0.0043 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.003 |
Peroxisomes | 0.0223 | 0.0221 | 0.0378 | 0.0431 | 0.0284 | 0.0232 | 0.0153 | 0.01 | 0.0211 | 0.0192 | 0.0018 | 0.0035 | 0.0199 | 0.0119 | 0.0274 | 0.004 | 0.0187 | 0.0226 |
Punctate Nuclear | 0.006 | 0.0001 | 0.0061 | 0.0001 | 0.0016 | 0.0002 | 0.0028 | 0.0002 | 0.0024 | 0.001 | 0 | 0.0002 | 0.0089 | 0.0002 | 0.0015 | 0.0001 | 0.0004 | 0.0008 |
Vacuole | 0.0021 | 0.0008 | 0.0017 | 0.0067 | 0.002 | 0.0006 | 0.004 | 0.0067 | 0.0086 | 0.0078 | 0.0023 | 0.0033 | 0.003 | 0.0023 | 0.001 | 0.0032 | 0.0005 | 0.0012 |
Vacuole Periphery | 0.0026 | 0.0008 | 0.0019 | 0.0045 | 0.0024 | 0.0014 | 0.0044 | 0.0036 | 0.0054 | 0.008 | 0.0035 | 0.0047 | 0.0037 | 0.0037 | 0.0016 | 0.0061 | 0.0011 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.9234 | 29.5274 | 23.6872 | 33.7655 | 27.7789 | 27.6591 | 29.25 | 33.4929 | 28.712 | 29.4262 |
Translational Efficiency | 2.2022 | 2.2351 | 2.4907 | 1.4944 | 2.0508 | 2.5659 | 2.0449 | 1.6288 | 1.8471 | 1.8707 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
142 | 223 | 1671 | 563 | 1922 | 1930 | 388 | 1268 | 2064 | 2153 | 2059 | 1831 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1350.52 | 1337.19 | 1445.05 | 1575.52 | 1201.55 | 1269.57 | 1567.50 | 1532.57 | 1211.80 | 1276.57 | 1468.12 | 1545.78 |
Standard Deviation | 202.60 | 226.07 | 201.94 | 238.95 | 191.74 | 191.93 | 180.52 | 250.15 | 196.17 | 196.83 | 203.79 | 247.55 |
Intensity Change Log 2 | — | -0.014311 | 0.097604 | 0.222313 | — | 0.079443 | 0.383569 | 0.351056 | — | 0.030590 | 0.239317 | 0.284360 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.012684 | 0.012173 | 0.034821 | 0.039182 | 0.008073 | 0.011963 | 0.019229 | 0.024586 | 0.008390 | 0.011985 | 0.031883 | 0.029074 |
Bud Neck | 0.002035 | 0.001583 | 0.002582 | 0.003481 | 0.000922 | 0.001048 | 0.006790 | 0.002627 | 0.000999 | 0.001104 | 0.003375 | 0.002889 |
Bud Site | 0.007063 | 0.004348 | 0.004342 | 0.044697 | 0.003017 | 0.003566 | 0.011954 | 0.038726 | 0.003296 | 0.003647 | 0.005777 | 0.040562 |
Cell Periphery | 0.000489 | 0.000192 | 0.000169 | 0.000566 | 0.000159 | 0.000206 | 0.000184 | 0.000387 | 0.000182 | 0.000205 | 0.000172 | 0.000442 |
Cytoplasm | 0.000915 | 0.002093 | 0.001216 | 0.008024 | 0.001143 | 0.002267 | 0.001237 | 0.012322 | 0.001128 | 0.002249 | 0.001220 | 0.011001 |
Cytoplasmic Foci | 0.141160 | 0.156907 | 0.159751 | 0.079284 | 0.196016* | 0.158851 | 0.209124* | 0.122568 | 0.192242* | 0.158650 | 0.169055 | 0.109259 |
Eisosomes | 0.000226 | 0.000134 | 0.000192 | 0.000159 | 0.000105 | 0.000134 | 0.000081 | 0.000100 | 0.000113 | 0.000134 | 0.000171 | 0.000118 |
Endoplasmic Reticulum | 0.002897 | 0.001300 | 0.001306 | 0.002071 | 0.000937 | 0.000955 | 0.001095 | 0.001086 | 0.001072 | 0.000991 | 0.001267 | 0.001389 |
Endosome | 0.139828 | 0.149463 | 0.103530 | 0.117463 | 0.105867 | 0.123727 | 0.168325 | 0.131826 | 0.108203 | 0.126393 | 0.115740 | 0.127409 |
Golgi | 0.573218* | 0.528837* | 0.525201* | 0.598671* | 0.458946* | 0.501170* | 0.421667* | 0.551546* | 0.466808* | 0.504035* | 0.505691* | 0.566036* |
Lipid Particles | 0.004569 | 0.027162 | 0.065699 | 0.010200 | 0.020250 | 0.017800 | 0.022971 | 0.005960 | 0.019172 | 0.018770 | 0.057647 | 0.007264 |
Mitochondria | 0.070476 | 0.064787 | 0.048553 | 0.008722 | 0.166584* | 0.130221* | 0.012407 | 0.015526 | 0.159972* | 0.123443* | 0.041741 | 0.013434 |
Mitotic Spindle | 0.005919 | 0.005484 | 0.009048 | 0.015116 | 0.005809 | 0.005550 | 0.050184 | 0.022676 | 0.005816 | 0.005544 | 0.016799 | 0.020352 |
None | 0.000109 | 0.000122 | 0.000157 | 0.000100 | 0.000203 | 0.000164 | 0.000072 | 0.000095 | 0.000196 | 0.000160 | 0.000141 | 0.000096 |
Nuclear Periphery | 0.000211 | 0.000357 | 0.001341 | 0.000295 | 0.000400 | 0.000347 | 0.001185 | 0.000159 | 0.000387 | 0.000348 | 0.001312 | 0.000201 |
Nuclear Periphery Foci | 0.000652 | 0.000801 | 0.001564 | 0.001656 | 0.001007 | 0.001260 | 0.004991 | 0.000880 | 0.000983 | 0.001213 | 0.002210 | 0.001119 |
Nucleolus | 0.000028 | 0.000047 | 0.000083 | 0.000038 | 0.000065 | 0.000139 | 0.000225 | 0.000054 | 0.000063 | 0.000130 | 0.000109 | 0.000049 |
Nucleus | 0.000038 | 0.000050 | 0.000050 | 0.000075 | 0.000062 | 0.000135 | 0.000103 | 0.000070 | 0.000060 | 0.000126 | 0.000060 | 0.000072 |
Peroxisomes | 0.006880 | 0.014969 | 0.007141 | 0.008994 | 0.005280 | 0.007850 | 0.010183 | 0.011387 | 0.005390 | 0.008587 | 0.007714 | 0.010652 |
Vacuole | 0.017826 | 0.015134 | 0.018390 | 0.043532 | 0.011463 | 0.016190 | 0.043927 | 0.042095 | 0.011900 | 0.016080 | 0.023203 | 0.042537 |
Vacuole Periphery | 0.012774 | 0.014056 | 0.014864 | 0.017673 | 0.013692 | 0.016454 | 0.014065 | 0.015323 | 0.013628 | 0.016206 | 0.014714 | 0.016046 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.09 | -4.04 | -3.69 | -4.73 | -0.74 | -2.84 | -2.68 | -6.02 | -4.48 | -1.09 | -2.74 | -10.15 | -8.03 | -6.53 | 0.89 |
Bud Neck | 0.78 | -1.07 | -2.57 | -3.92 | -2.16 | -0.85 | -5.86 | -6.59 | -5.96 | 3.84 | -0.73 | -8.15 | -8.38 | -7.71 | 0.82 |
Bud Site | 0.97 | 1.01 | -7.29 | -8.71 | -8.83 | -1.15 | -4.62 | -13.59 | -13.44 | -8.45 | -0.74 | -3.90 | -16.13 | -16.09 | -14.91 |
Cell Periphery | 1.12 | 1.20 | -0.31 | -7.46 | -8.45 | -3.37 | -1.62 | -8.77 | -6.79 | -7.29 | -1.03 | 0.45 | -8.79 | -9.78 | -11.65 |
Cytoplasm | -2.02 | -1.36 | -5.41 | -4.32 | -5.22 | -1.81 | -0.77 | -8.98 | -7.34 | -8.91 | -1.99 | -0.74 | -10.36 | -8.09 | -10.25 |
Cytoplasmic Foci | -1.16 | -1.71 | 5.54 | 8.03 | 15.34 | 7.81 | -1.48 | 13.75 | 7.05 | 9.42 | 7.44 | 5.03 | 18.12 | 11.48 | 13.63 |
Eisosomes | 0.81 | 0.30 | 0.59 | -0.86 | 1.32 | -4.11 | 3.21 | 0.80 | 4.02 | -2.03 | -2.03 | -5.46 | -0.33 | 1.62 | 5.01 |
Endoplasmic Reticulum | 1.07 | 1.10 | 0.56 | -1.37 | -1.73 | -0.21 | -1.13 | -0.97 | -0.74 | 0.06 | 0.53 | -1.04 | -1.63 | -2.15 | -0.53 |
Endosome | -0.63 | 3.01 | 1.82 | 2.96 | -2.37 | -4.57 | -8.70 | -5.94 | -1.73 | 4.95 | -4.78 | -1.99 | -4.92 | -0.13 | -2.86 |
Golgi | 1.51 | 1.99 | -0.65 | -2.82 | -4.59 | -5.06 | 2.56 | -8.36 | -4.14 | -7.50 | -4.63 | -4.58 | -10.18 | -5.98 | -5.75 |
Lipid Particles | -3.92 | -16.76 | -3.00 | 2.79 | 14.14 | 1.16 | -0.83 | 8.53 | 7.09 | 5.23 | 0.21 | -11.90 | 7.38 | 6.90 | 16.70 |
Mitochondria | 0.32 | 1.57 | 4.52 | 5.00 | 10.94 | 4.66 | 24.13 | 24.42 | 21.29 | -1.00 | 4.98 | 19.01 | 25.37 | 22.43 | 9.49 |
Mitotic Spindle | 0.15 | -1.20 | -2.99 | -3.82 | -2.63 | 0.24 | -6.17 | -7.84 | -7.77 | 3.57 | 0.27 | -6.28 | -8.54 | -8.49 | -1.95 |
None | -0.74 | -2.84 | 0.47 | 1.41 | 4.15 | 3.28 | 11.54 | 9.51 | 6.81 | -2.52 | 3.34 | 4.29 | 9.95 | 7.27 | 4.08 |
Nuclear Periphery | -1.55 | -5.41 | -1.01 | 0.45 | 4.80 | 0.48 | -3.85 | 2.74 | 2.65 | 5.41 | 0.38 | -5.04 | 2.16 | 2.13 | 6.51 |
Nuclear Periphery Foci | -0.47 | -2.78 | -1.82 | -1.65 | -0.35 | -0.47 | -2.55 | 0.22 | 0.69 | 2.64 | -0.47 | -2.80 | -0.61 | 0.04 | 2.58 |
Nucleolus | -1.11 | -2.40 | -1.29 | 0.22 | 1.75 | -1.45 | -1.63 | 0.05 | 1.48 | 1.64 | -1.46 | -1.67 | 0.28 | 1.60 | 1.94 |
Nucleus | -0.86 | -1.44 | -2.98 | -1.95 | -2.28 | -1.02 | -1.45 | -1.48 | 0.21 | -0.33 | -1.02 | -0.11 | -1.75 | 0.27 | -1.73 |
Peroxisomes | -1.68 | -0.12 | -0.78 | 1.31 | -1.06 | -2.65 | -3.26 | -5.14 | -2.66 | -0.66 | -3.21 | -2.92 | -5.15 | -1.69 | -2.72 |
Vacuole | 0.55 | -0.17 | -4.85 | -6.23 | -6.41 | -3.65 | -8.23 | -12.54 | -10.32 | -0.03 | -3.37 | -8.21 | -14.44 | -12.12 | -8.75 |
Vacuole Periphery | -0.33 | -0.82 | -1.62 | -1.01 | -1.42 | -2.61 | -0.24 | -1.47 | 0.93 | -0.77 | -2.51 | -1.15 | -2.31 | 0.16 | -1.21 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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