Standard name
Human Ortholog
Description Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.37 0.17 0.11 0 0 0 0 0 0 0 0.08 0.08 0.07 0.07 0 0.44 0.5 0.38 0.21 0 0 0.1 0.12 0.08
Cytoplasm 0.68 0.89 0.95 1.0 1.0 0.99 0.95 0.96 0.96 0.92 0.9 0.9 0.96 0.96 0.98 0.64 0.63 0.7 0.74 0.95 0.95 0.85 0.82 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 45 22 6 6 5 8 12 3 9 14 17 9 9 5 86 45 55 15 7 2 11 15 13
Cytoplasm 26 232 193 152 234 238 280 316 209 190 157 200 116 133 145 125 57 101 52 362 256 100 107 141
Endoplasmic Reticulum 0 0 0 0 0 2 12 4 6 11 10 12 0 0 0 2 4 7 0 2 2 0 2 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 1
Unique Cell Count 38 260 204 152 235 241 296 328 218 206 174 222 121 138 148 195 90 144 71 384 269 118 132 163
Labelled Cell Count 40 277 215 158 240 245 301 335 219 211 184 236 125 142 150 213 106 163 71 384 269 118 132 163


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 642.1 498.2 433.9 362.6 319.5 245.7 199.6 171.8 150.5 130.8 118.8 105.8 496.7 467.1 422.3 603.9 594.6 592.0 551.4 559.2 552.9
Std Deviation (1e-4) 60.8 90.0 83.2 74.9 64.8 64.3 52.6 45.6 42.0 45.1 43.0 42.3 84.6 90.0 77.0 127.1 149.8 138.4 160.5 159.0 124.2
Intensity Change (Log2) – – – -0.26 -0.44 -0.82 -1.12 -1.34 -1.53 -1.73 -1.87 -2.04 0.19 0.11 -0.04 0.48 0.45 0.45 0.35 0.37 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -3.5 -3.8 -3.7 -3.3 -4.1 -2.5 -0.9 -1.1 -1.0 -1.3 -2.6 7.5 7.4 6.1
Cytoplasm 2.9 3.2 2.5 0 1.0 0.6 -1.0 -1.6 -1.7 0.5 0.8 1.6 -7.6 -6.9 -6.2
Endoplasmic Reticulum 0 0 0 0 0 0 3.3 0 3.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 586.5545 871.3488 642.5129 760.4482 405.8297 238.906 433.8122 697.7408 580.1685 387.145 486.4264 586.7285 605.5671 828.3716 710.1399 541.9143 596.3872 630.6699
Actin 0.0231 0.0002 0.0027 0.0001 0.1055 0.0054 0.0041 0.0001 0.004 0.0003 0.0002 0.0002 0.001 0.0001 0.0002 0.0006 0.0001 0.0002
Bud 0.0016 0.0004 0.0007 0.0001 0.0009 0.0098 0.0015 0.0003 0.0008 0.0006 0.0002 0.0001 0.0007 0.0002 0.0003 0.0002 0.0003 0.0001
Bud Neck 0.0005 0.0001 0.0003 0.0002 0.0004 0.008 0.0004 0.0001 0.0004 0.0004 0.0006 0.0007 0.0002 0.0001 0.0003 0.0004 0.0002 0.0011
Bud Periphery 0.0008 0.0001 0.0002 0 0.0006 0.0082 0.0008 0 0.0004 0.0005 0.0001 0 0.0005 0 0.0001 0 0.0001 0
Bud Site 0.0015 0.0001 0.0006 0 0.0007 0.0039 0.0013 0.0001 0.001 0.0016 0.0001 0 0.0005 0.0001 0 0 0.0001 0
Cell Periphery 0.0006 0.0018 0.0007 0.0001 0.0009 0.0171 0.0038 0.0016 0.0009 0.0024 0.0013 0.0004 0.0007 0 0.0001 0.0006 0.0009 0.0001
Cytoplasm 0.7693 0.9782 0.8228 0.9858 0.5245 0.4726 0.7593 0.9848 0.9497 0.6246 0.7779 0.9515 0.9019 0.9916 0.989 0.9648 0.7345 0.8921
Cytoplasmic Foci 0.0334 0.0001 0.0454 0 0.0169 0.0421 0.0286 0.0001 0.0024 0.0414 0.0032 0.0003 0.0145 0 0.0001 0.0002 0.0626 0.0514
Eisosomes 0.001 0 0.0008 0 0.0024 0.0003 0.0014 0.0001 0.0001 0.0037 0.0002 0 0.0006 0 0 0 0.0019 0.0003
Endoplasmic Reticulum 0.0018 0 0.0022 0 0.0015 0.0212 0.002 0.0001 0.0007 0.0024 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0001 0.0001
Endosome 0.0102 0 0.0037 0 0.032 0.0143 0.0087 0 0.0013 0.0439 0.0001 0 0.0031 0 0 0 0.0001 0.0001
Golgi 0.0059 0 0.0009 0 0.0103 0.0016 0.0047 0 0.0006 0.0018 0 0 0.0005 0 0 0 0.0001 0
Lipid Particles 0.0135 0 0.006 0 0.0379 0.0031 0.0097 0.0001 0.0006 0.0121 0.0012 0.0001 0.002 0 0 0.0001 0.0055 0.0019
Mitochondria 0.0276 0.0009 0.0104 0.0002 0.0869 0.0563 0.0177 0.0004 0.0087 0.0408 0.0104 0.0003 0.0107 0.0003 0.0005 0.0002 0.0381 0.01
None 0.0151 0.0008 0.013 0.0002 0.0083 0.0539 0.0254 0.0009 0.0038 0.0376 0.0114 0.0007 0.0088 0.0002 0.0002 0.0004 0.0651 0.0025
Nuclear Periphery 0.0071 0.0014 0.0059 0.0027 0.0171 0.0308 0.0087 0.0019 0.0038 0.0067 0.0065 0.0076 0.0036 0.0005 0.0013 0.0054 0.0015 0.0037
Nucleolus 0.0278 0.001 0.0257 0 0.103 0.0227 0.0651 0.0006 0.0037 0.1098 0.1337 0.0013 0.0289 0 0 0.0001 0.0549 0.0154
Nucleus 0.0401 0.0132 0.0285 0.0103 0.0324 0.0642 0.0412 0.0076 0.0148 0.0267 0.0402 0.0352 0.0171 0.0067 0.0071 0.0261 0.0258 0.018
Peroxisomes 0.0133 0 0.0228 0 0.0037 0.0033 0.0034 0 0.0004 0.0022 0.0002 0 0.0008 0 0 0 0.0027 0.0007
Punctate Nuclear 0.0036 0 0.0016 0 0.0015 0.0927 0.0023 0 0.0003 0.0014 0.0009 0.0003 0.0026 0 0 0.0001 0.0009 0.0002
Vacuole 0.0011 0.0014 0.0024 0.0001 0.0043 0.0536 0.0058 0.001 0.0013 0.0311 0.005 0.0007 0.0006 0.0001 0.0005 0.0005 0.0024 0.0008
Vacuole Periphery 0.0011 0.0003 0.0027 0.0001 0.0084 0.0149 0.0042 0.0001 0.0004 0.0082 0.0065 0.0003 0.0006 0 0.0001 0.0002 0.0024 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 223.0146 157.7849 165.4863 200.0931 171.3155 261.4905 228.5348 219.5631 202.478 199.089
Translational Efficiency 1.3813 1.5342 1.3082 1.1834 1.2213 1.3138 1.1371 0.869 0.9433 0.885

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1741 1053 250 1257 1081 1812 642 334 2822 2865 892 1591

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 21296.64 24544.34 16208.66 6400.03 18667.01 31463.59 7972.92 12776.91 20289.33 28920.49 10281.14 7738.73
Standard Deviation 5512.07 5834.16 3299.26 1781.91 4049.30 7591.04 1741.51 3342.39 5163.29 7751.38 4349.21 3405.66
Intensity Change Log 2 — 0.204765 -0.393861 -1.734475 — 0.753192 -1.227311 -0.546952 — 0.486943 -0.724780 -1.059316

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000015 0.000040 0.000116 0.000132 0.000026 0.000034 0.000122 0.000505 0.000019 0.000037 0.000120 0.000210
Bud Neck 0.000071 0.000258 0.002678 0.002402 0.000198 0.000186 0.002596 0.002876 0.000120 0.000213 0.002619 0.002501
Bud Site 0.000226 0.000990 0.001128 0.006736 0.000603 0.000554 0.002326 0.007820 0.000371 0.000714 0.001990 0.006963
Cell Periphery 0.000777 0.003652 0.028842 0.004678 0.005361 0.003558 0.019988 0.045014 0.002533 0.003593 0.022470 0.013146
Cytoplasm 0.890136* 0.861544* 0.636057* 0.821510* 0.895740* 0.883229* 0.745294* 0.635536* 0.892283* 0.875259* 0.714678* 0.782468*
Cytoplasmic Foci 0.001776 0.004774 0.030157 0.004736 0.004358 0.003586 0.012607 0.006827 0.002765 0.004022 0.017525 0.005175
Eisosomes 0.000000 0.000001 0.000008 0.000002 0.000001 0.000001 0.000004 0.000012 0.000001 0.000001 0.000005 0.000004
Endoplasmic Reticulum 0.000007 0.000017 0.000033 0.000091 0.000024 0.000016 0.000066 0.000325 0.000013 0.000016 0.000056 0.000140
Endosome 0.000010 0.000029 0.000107 0.000625 0.000027 0.000019 0.000221 0.003038 0.000017 0.000022 0.000189 0.001131
Golgi 0.001561 0.001544 0.007234 0.002941 0.003118 0.001177 0.010338 0.004139 0.002157 0.001312 0.009468 0.003193
Lipid Particles 0.000362 0.001544 0.009105 0.005981 0.002025 0.001195 0.015156 0.025436 0.000999 0.001323 0.013460 0.010065
Mitochondria 0.000088 0.000088 0.000169 0.000553 0.000082 0.000103 0.000107 0.002592 0.000086 0.000097 0.000124 0.000981
Mitotic Spindle 0.000118 0.000219 0.000509 0.002105 0.000105 0.000097 0.001363 0.002468 0.000113 0.000141 0.001124 0.002181
None 0.017750 0.010496 0.006617 0.003917 0.013451 0.015140 0.002682 0.001820 0.016103 0.013433 0.003785 0.003477
Nuclear Periphery 0.000091 0.000155 0.002312 0.000199 0.000189 0.000121 0.001191 0.000545 0.000128 0.000134 0.001505 0.000272
Nuclear Periphery Foci 0.000738 0.001189 0.003998 0.002286 0.001715 0.000879 0.007265 0.003904 0.001112 0.000993 0.006349 0.002626
Nucleolus 0.000159 0.000053 0.000093 0.000204 0.000042 0.000052 0.000076 0.000132 0.000114 0.000052 0.000081 0.000189
Nucleus 0.082200 0.099958 0.202212* 0.053914 0.060465 0.078976 0.073639 0.031264 0.073874 0.086688 0.109674 0.049159
Peroxisomes 0.000012 0.000007 0.000006 0.000053 0.000008 0.000006 0.000009 0.000130 0.000010 0.000006 0.000008 0.000069
Vacuole 0.002728 0.011935 0.061690 0.083764 0.010794 0.010062 0.097345 0.220669* 0.005818 0.010750 0.087352 0.112505
Vacuole Periphery 0.001176 0.001509 0.006928 0.003171 0.001669 0.001010 0.007606 0.004949 0.001365 0.001193 0.007416 0.003544

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.66 -8.39 -10.72 -7.51 0.03 -1.50 -11.58 -2.36 -2.32 -1.56 -4.97 -14.63 -5.10 -4.55 -1.29
Bud Neck -6.75 -6.55 -9.79 -9.00 0.29 0.48 -7.13 -5.09 -5.12 0.68 -5.06 -9.32 -10.91 -10.48 0.57
Bud Site -4.75 -5.92 -8.37 -7.16 -6.88 0.67 -6.12 -5.65 -5.80 -4.03 -3.02 -8.72 -9.78 -9.26 -6.69
Cell Periphery -5.27 -8.38 -7.39 0.17 7.47 2.22 -8.37 -7.57 -8.15 -3.68 -2.72 -13.29 -8.03 -6.68 7.10
Cytoplasm 6.88 17.50 17.43 10.51 -9.71 2.32 15.94 16.17 15.63 4.42 6.00 22.73 22.76 19.12 -5.68
Cytoplasmic Foci -5.93 -9.48 -7.38 -0.91 8.09 1.25 -7.92 -2.49 -3.19 3.70 -3.88 -13.14 -6.30 -3.67 9.80
Eisosomes -4.35 -6.91 -9.40 -6.77 5.15 1.01 -9.26 -3.34 -3.42 -1.92 -2.41 -11.71 -5.92 -5.50 2.58
Endoplasmic Reticulum -3.56 -6.05 -3.47 -2.89 -1.71 1.79 -6.66 -1.15 -1.20 -0.88 -1.51 -10.39 -2.33 -2.25 -1.08
Endosome -3.37 -6.48 -3.31 -3.17 -2.51 1.55 -8.54 -1.21 -1.21 -1.06 -1.96 -10.17 -2.26 -2.24 -1.65
Golgi -0.01 -6.70 -2.08 -2.58 5.59 3.41 -7.91 -0.29 -4.15 7.99 2.48 -11.35 -1.38 -4.26 10.56
Lipid Particles -4.81 -6.52 -10.08 -7.40 2.08 2.12 -9.58 -7.85 -8.22 -2.04 -1.95 -12.01 -11.91 -11.31 3.27
Mitochondria -0.13 -3.67 -1.19 -1.18 -0.76 -1.91 -4.54 -1.07 -1.06 -1.04 -0.91 -3.79 -1.53 -1.49 -1.36
Mitotic Spindle -1.17 -2.87 -2.63 -2.50 -2.04 -0.03 -3.77 -1.19 -1.19 0.03 -0.73 -4.10 -2.89 -2.84 -0.97
None 5.87 8.99 13.03 7.58 2.91 -1.06 9.20 9.54 12.36 2.20 2.80 15.35 15.41 13.06 1.65
Nuclear Periphery -2.19 -7.32 -4.36 -2.99 6.86 2.78 -8.08 -5.11 -6.63 5.58 -0.66 -11.04 -5.96 -7.24 9.81
Nuclear Periphery Foci -2.20 -5.95 -7.08 -5.00 2.88 3.15 -9.48 -4.44 -7.08 6.12 0.23 -11.87 -7.64 -8.41 7.95
Nucleolus 0.79 0.42 -0.45 -6.87 -4.69 -1.04 -6.24 -3.75 -3.27 -1.86 0.74 0.23 -1.06 -7.47 -5.47
Nucleus -5.04 -12.42 3.33 7.29 13.40 -5.24 -5.08 4.59 9.18 8.36 -5.76 -9.90 3.63 8.37 11.50
Peroxisomes 0.50 0.48 -2.87 -5.43 -5.49 0.83 -1.27 -2.25 -2.30 -2.18 0.67 0.14 -4.02 -5.14 -4.82
Vacuole -6.73 -8.73 -22.42 -19.02 -4.11 0.80 -14.13 -16.99 -17.18 -8.50 -4.89 -17.24 -26.77 -25.40 -5.32
Vacuole Periphery -0.39 -3.78 -2.72 -1.26 2.86 1.38 -6.23 -2.58 -3.49 3.06 0.49 -7.72 -3.36 -3.33 5.51
Standard name
Human Ortholog
Description Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Cpa2

Cpa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available