Standard name
Human Ortholog
Description Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.96 0.98 0.99 0.96 0.87 0.85 0.77 0.79 0.69 0.73 0.98 0.99 0.98 0.77 0.74 0.73 0.9 0.9 0.88 0.86 0.89 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.06 0 0.14 0.36 0.23 0.44 0.33 0.5 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0.39 0.31 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1 1
Bud 0 0 0 0 0 1 0 1 7 2 8 7 0 0 1 0 0 0 0 0 0 2 3 4
Bud Neck 0 0 0 2 1 2 1 2 1 3 2 2 1 0 0 0 0 0 0 1 0 0 1 2
Bud Site 0 0 0 0 0 0 1 0 0 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 0 1 3 0 1 0 2 3 0 0 0 0 0 0 0
Cytoplasm 224 201 107 168 196 284 251 214 212 254 221 188 195 234 212 157 164 205 217 191 128 167 296 262
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 1 1 0 0 6 4 6 0 0 0 1 1 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 2 3 3 2 2 1 1 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 0 0 2 0
Mitochondria 19 3 4 11 4 42 103 57 123 105 159 92 0 0 0 3 3 4 4 3 6 4 3 4
Nucleus 0 2 1 0 0 1 2 1 1 0 4 5 3 1 3 9 1 6 0 4 0 0 0 2
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 2 0 3 2 1 0 0 0 0 0 0 1 1 1 2 3 1
Vac/Vac Membrane 9 3 1 2 0 2 1 2 6 11 7 9 3 6 9 81 87 87 6 5 2 3 8 3
Unique Cell Count 238 206 112 171 198 297 289 253 277 320 318 259 198 236 217 205 221 279 243 213 146 194 331 293
Labelled Cell Count 254 211 114 183 202 333 360 281 350 379 406 310 203 242 225 262 264 311 243 213 146 194 331 293


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.9 4.7 4.5 4.7 3.7 3.4 4.2 3.3 3.5 3.2 3.6 4.8 5.4 5.5 7.1 6.9 6.6 4.9 5.2 5.7
Std Deviation (1e-4) 0.7 0.6 1.2 1.3 1.6 1.3 1.0 1.5 1.1 1.0 1.1 0.9 0.6 1.2 1.7 1.0 1.4 1.4 1.6 1.4 1.8
Intensity Change (Log2) – – – -0.04 0.02 -0.32 -0.44 -0.16 -0.49 -0.4 -0.55 -0.38 0.06 0.21 0.24 0.62 0.58 0.51 0.06 0.17 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 2.0 0 -2.5 -3.0 -4.4 -4.0 -5.6 -5.0 1.6 2.2 1.1 -4.3 -4.7 -4.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 7.5 7.5 6.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8633 2.072 1.6969 1.7569 1.7683 1.7043 -0.0081 0.2279 0.0667 0.1074 -0.2461 0.1139 -0.3774 -0.2818 -0.5371 -0.189 -1.4105 -0.4019
Actin 0.0096 0.0005 0.0033 0.0009 0.0072 0.0017 0.0215 0.0007 0.0062 0.0174 0.0007 0.0029 0.0449 0.009 0.0084 0.0002 0.0001 0.0019
Bud 0.0007 0.0003 0.0009 0.0001 0.001 0.0005 0.0025 0.0017 0.0086 0.001 0.0003 0.0006 0.0013 0.0012 0.0015 0.0005 0 0.0012
Bud Neck 0.0034 0.0003 0.0015 0.0007 0.001 0.0028 0.0069 0.0004 0.0022 0.0024 0.0007 0.0015 0.0027 0.0001 0.0002 0.0002 0.0001 0.0008
Bud Periphery 0.0007 0.0003 0.0012 0.0001 0.001 0.0006 0.0028 0.0008 0.0041 0.0013 0.0003 0.0007 0.0017 0.0012 0.0071 0.0008 0 0.0017
Bud Site 0.0075 0.0009 0.0041 0.0006 0.0009 0.001 0.0047 0.012 0.009 0.0119 0.0002 0.0002 0.0067 0.0056 0.0006 0.0025 0 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0005 0.0003 0 0.0001 0.0004 0.0001 0.0003 0.0004 0 0
Cytoplasm 0.3224 0.3311 0.3262 0.3873 0.3582 0.4362 0.1898 0.2528 0.2155 0.1986 0.266 0.2856 0.2224 0.336 0.4036 0.3645 0.5501 0.5369
Cytoplasmic Foci 0.0516 0.005 0.0221 0.0285 0.0406 0.0122 0.0221 0.0056 0.0112 0.0193 0.0124 0.0116 0.0475 0.0105 0.0059 0.0072 0.0041 0.0317
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0001 0.0003 0 0.0001 0.0007 0.0001 0.0004 0 0 0
Endoplasmic Reticulum 0.007 0.0045 0.0032 0.0021 0.0036 0.0039 0.0037 0.0038 0.0029 0.0033 0.001 0.0017 0.0073 0.0021 0.0088 0.004 0.0023 0.0037
Endosome 0.0466 0.0086 0.0197 0.0412 0.068 0.0067 0.0126 0.0023 0.0075 0.0186 0.0014 0.0061 0.0355 0.002 0.0062 0.0372 0.0014 0.014
Golgi 0.0109 0.001 0.0078 0.0117 0.0417 0.0037 0.0027 0.0001 0.002 0.0098 0.0002 0.0009 0.0159 0.0004 0.0015 0.0021 0 0.0117
Lipid Particles 0.0102 0.0003 0.0129 0.0061 0.0063 0.0026 0.0085 0.0003 0.0036 0.0124 0.0004 0.001 0.0114 0.0004 0.0007 0.0016 0 0.0025
Mitochondria 0.0104 0.0096 0.0078 0.0015 0.0557 0.0027 0.0067 0.0006 0.0093 0.0544 0.0004 0.0034 0.0178 0.0015 0.0155 0.0035 0.0001 0.0009
None 0.3931 0.4154 0.4586 0.2602 0.1468 0.3091 0.6597 0.6815 0.6456 0.53 0.6778 0.6194 0.5425 0.6147 0.5242 0.5188 0.4312 0.3702
Nuclear Periphery 0.02 0.0092 0.0057 0.0039 0.0073 0.0075 0.0124 0.0042 0.0098 0.0118 0.0016 0.0081 0.006 0.0018 0.0033 0.0045 0.0013 0.0024
Nucleolus 0.0008 0.0005 0.0015 0.0004 0.0007 0.0017 0.0019 0.001 0.0013 0.007 0.0005 0.0011 0.0006 0.0002 0.0002 0.0008 0 0.0003
Nucleus 0.0704 0.205 0.0957 0.2367 0.2204 0.1729 0.0171 0.0212 0.0318 0.0316 0.0051 0.0244 0.0171 0.0055 0.0052 0.0042 0.0071 0.0096
Peroxisomes 0.0172 0.0005 0.0154 0.01 0.0209 0.0053 0.0069 0.0003 0.0034 0.0269 0.0016 0.0007 0.0107 0.0024 0.0004 0.0002 0 0.0026
Punctate Nuclear 0.0104 0.0037 0.0085 0.0056 0.011 0.027 0.0137 0.0086 0.0232 0.0363 0.0288 0.0284 0.003 0.0039 0.0011 0.0012 0.0014 0.0061
Vacuole 0.0044 0.0023 0.0031 0.002 0.0042 0.0014 0.0021 0.0014 0.0016 0.0041 0.0004 0.0009 0.0024 0.0013 0.003 0.0379 0.0006 0.0013
Vacuole Periphery 0.0023 0.0008 0.0007 0.0004 0.0036 0.0003 0.0009 0.0003 0.0007 0.0013 0.0001 0.0008 0.0013 0.0002 0.0017 0.0077 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.431 9.2357 12.336 11.4168 9.3767 22.7167 10.5055 11.3448 15.6273 13.3474
Translational Efficiency 1.2571 2.4619 1.2185 2.3632 3.3281 0.9374 2.0534 2.1107 1.3168 1.1876

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1948 1375 416 134 2109 2017 1261 1054 4057 3392 1677 1188

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.77 730.68 825.01 896.32 673.32 733.67 836.54 868.75 675.94 732.46 833.68 871.86
Standard Deviation 74.99 91.13 82.42 100.80 76.14 87.31 99.09 122.23 75.64 88.89 95.36 120.32
Intensity Change Log 2 — 0.106317 0.281489 0.401091 — 0.123839 0.313142 0.367649 — 0.115069 0.297339 0.384534

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000102 0.000437 0.000208 0.001444 0.000204 0.000369 0.000214 0.000625 0.000155 0.000397 0.000212 0.000717
Bud Neck 0.015518 0.026425 0.001775 0.004670 0.008408 0.021019 0.001422 0.006549 0.011822 0.023210 0.001509 0.006337
Bud Site 0.003903 0.013238 0.001275 0.025431 0.004081 0.012581 0.002367 0.028591 0.003995 0.012847 0.002096 0.028235
Cell Periphery 0.000086 0.000121 0.000156 0.000254 0.000117 0.000112 0.000140 0.000212 0.000102 0.000116 0.000144 0.000217
Cytoplasm 0.473666* 0.425618* 0.189751* 0.322902* 0.500766* 0.507358* 0.270433* 0.377773* 0.487754* 0.474223* 0.250418* 0.371584*
Cytoplasmic Foci 0.165224 0.207295* 0.010001 0.009352 0.106584 0.133418 0.006062 0.016704 0.134740 0.163365 0.007039 0.015875
Eisosomes 0.000090 0.000098 0.000051 0.000109 0.000065 0.000075 0.000031 0.000041 0.000077 0.000084 0.000036 0.000049
Endoplasmic Reticulum 0.000659 0.001261 0.003418 0.011932 0.000689 0.000907 0.002975 0.003065 0.000675 0.001051 0.003085 0.004065
Endosome 0.007117 0.016239 0.002322 0.005516 0.003472 0.008645 0.001186 0.007049 0.005222 0.011723 0.001468 0.006876
Golgi 0.001224 0.002949 0.000062 0.000255 0.000552 0.002639 0.000053 0.015190 0.000875 0.002765 0.000055 0.013505
Lipid Particles 0.005833 0.006364 0.002069 0.002954 0.004504 0.003881 0.000972 0.001131 0.005142 0.004887 0.001244 0.001336
Mitochondria 0.004270 0.009912 0.000461 0.015559 0.002541 0.007932 0.000379 0.003361 0.003371 0.008735 0.000399 0.004737
Mitotic Spindle 0.001770 0.006060 0.000163 0.003348 0.002339 0.008479 0.001871 0.048614 0.002066 0.007499 0.001448 0.043508
None 0.003226 0.004577 0.005432 0.007360 0.004750 0.004107 0.006192 0.007294 0.004018 0.004297 0.006003 0.007302
Nuclear Periphery 0.000586 0.000554 0.001588 0.002265 0.000777 0.000520 0.001356 0.001924 0.000685 0.000534 0.001413 0.001962
Nuclear Periphery Foci 0.000273 0.000868 0.000890 0.003169 0.000416 0.000501 0.000825 0.001040 0.000347 0.000650 0.000841 0.001280
Nucleolus 0.001355 0.002949 0.001111 0.000580 0.000937 0.002063 0.000563 0.000460 0.001138 0.002422 0.000699 0.000474
Nucleus 0.273415* 0.198397* 0.742070* 0.395233* 0.338426* 0.233388* 0.676267* 0.396672* 0.307210* 0.219204* 0.692590* 0.396509*
Peroxisomes 0.004087 0.013754 0.000283 0.001078 0.002845 0.007426 0.000132 0.001716 0.003442 0.009991 0.000169 0.001644
Vacuole 0.036953 0.060956 0.036672 0.185395 0.016876 0.043277 0.026449 0.075713 0.026516 0.050444 0.028985 0.088085
Vacuole Periphery 0.000645 0.001930 0.000244 0.001196 0.000653 0.001302 0.000113 0.006275 0.000649 0.001556 0.000146 0.005702

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.83 -5.57 -5.01 -2.69 -3.84 -1.42 -0.82 -2.65 -1.33 -2.99 -3.25 -2.45 -4.88 -1.84 -3.91
Bud Neck -4.97 11.13 8.27 11.03 -3.52 -7.65 7.49 2.10 9.87 -8.75 -8.55 13.67 6.79 14.09 -9.01
Bud Site -7.09 3.26 -1.73 0.86 -2.31 -6.40 1.93 -4.57 -0.71 -5.38 -9.38 2.93 -4.95 -0.48 -5.80
Cell Periphery -4.16 -4.49 -5.65 -4.04 -1.48 0.59 -2.14 -1.75 -2.04 -0.26 -2.39 -4.90 -3.52 -2.56 -0.29
Cytoplasm 5.42 25.46 12.33 9.73 -1.33 -0.46 29.66 24.51 24.00 -2.47 2.66 37.39 27.14 24.00 -3.87
Cytoplasmic Foci -5.88 33.85 34.99 34.75 1.90 -5.33 31.08 29.27 31.49 -3.45 -6.66 46.15 44.30 45.51 -2.29
Eisosomes -1.20 3.31 -3.19 -2.40 -5.49 -2.21 7.47 0.07 2.30 -7.76 -1.87 8.58 1.51 3.42 -7.88
Endoplasmic Reticulum -6.40 -10.83 -10.25 -9.70 -6.37 -1.16 -15.31 -19.18 -14.37 -4.06 -3.06 -18.91 -21.33 -18.02 -6.10
Endosome -6.69 6.49 4.54 9.80 -1.46 -7.41 5.82 -0.00 6.75 -4.37 -9.19 9.61 3.26 11.19 -4.13
Golgi -4.87 6.30 5.87 9.39 -2.49 -4.59 5.31 -2.34 -0.92 -2.68 -6.11 8.12 -2.06 -0.64 -2.69
Lipid Particles -0.99 7.72 6.22 5.96 -1.37 1.11 8.00 7.08 6.36 -3.57 0.49 12.59 11.91 10.35 -2.22
Mitochondria -3.78 5.72 0.34 2.20 -1.10 -4.62 4.68 1.51 5.48 -2.50 -5.78 7.44 2.51 7.14 -2.66
Mitotic Spindle -2.80 1.79 -1.37 0.21 -2.00 -3.90 -0.41 -6.46 -4.44 -6.26 -4.80 -0.08 -6.62 -4.74 -6.56
None -1.77 -1.79 -1.26 -0.00 0.30 0.89 -0.97 -1.33 -2.27 -0.37 -0.53 -2.56 -3.06 -2.13 -0.37
Nuclear Periphery 0.43 -13.31 -12.21 -12.36 -5.30 2.28 -8.09 -11.55 -13.57 -7.89 2.30 -14.19 -13.88 -14.97 -8.42
Nuclear Periphery Foci -3.40 -6.24 -6.45 -4.55 -4.26 -0.54 -5.08 -7.40 -6.67 -3.18 -2.86 -7.95 -10.26 -6.90 -4.44
Nucleolus -4.40 1.78 6.63 7.22 3.80 -3.91 3.65 3.51 5.20 -0.42 -5.66 4.88 6.24 8.30 1.13
Nucleus 9.70 -32.82 -6.73 -9.62 10.35 14.12 -37.05 -9.71 -19.90 20.20 16.33 -51.42 -14.40 -23.67 23.32
Peroxisomes -8.08 10.96 9.91 11.67 -0.43 -5.82 6.20 4.59 9.85 -1.95 -9.76 11.67 8.38 14.76 -1.86
Vacuole -8.49 -3.93 -10.66 -9.54 -9.28 -15.21 -10.26 -24.23 -20.22 -19.20 -15.03 -8.60 -25.19 -21.47 -20.81
Vacuole Periphery -5.21 4.02 1.63 5.54 -0.84 -2.58 2.91 -1.36 -0.59 -2.08 -5.25 4.85 -1.35 -0.11 -2.08
Standard name
Human Ortholog
Description Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Arg82

Arg82


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg82-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available