Standard name
Human Ortholog
Description Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.9 – 0.95 0.94 0.96 0.86 0.84 0.88 0.73 0.8 0.61 0.74 1.0 0.99 1.0 – – – 0.72 0.75 0.65 0.84 0.84 0.73
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.19 – 0.1 0.2 0.05 0.21 0.29 0.17 0.42 0.4 0.54 0.5 0 0 0 – – – 0.14 0.11 0.16 0.06 0.07 0.11
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 0 3 3 4 8
Bud 0 – 3 3 0 5 0 2 6 1 1 3 0 1 0 – – – 0 0 0 1 6 7
Bud Neck 1 – 2 1 3 3 5 7 7 2 1 3 0 0 0 – – – 0 0 2 0 2 9
Bud Site 0 – 0 0 0 0 0 0 2 0 0 1 0 0 0 – – – – – – – – –
Cell Periphery 0 – 0 0 1 0 2 0 0 1 2 1 0 0 1 – – – 0 0 2 1 6 10
Cytoplasm 203 – 143 130 182 148 244 307 163 207 102 184 322 495 639 – – – 162 42 93 143 395 336
Endoplasmic Reticulum 1 – 0 0 1 1 1 0 1 1 0 0 1 2 0 – – – 2 0 2 1 1 2
Endosome 0 – 0 1 0 1 0 1 0 0 0 0 0 0 0 – – – 7 1 3 1 2 4
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 – – – 0 0 0 0 2 0
Mitochondria 44 – 15 27 10 36 85 58 94 102 90 123 0 3 0 – – – 30 6 23 9 32 51
Nucleus 0 – 1 0 0 2 3 4 4 1 2 1 0 1 0 – – – 0 0 0 0 0 0
Nuclear Periphery 1 – 0 0 1 0 0 0 0 1 1 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 4 5 6 4 1 4 8 0 1 0 – – – 3 0 2 2 9 7
Vac/Vac Membrane 0 – 0 1 0 1 0 1 1 1 1 0 0 0 0 – – – 7 4 5 0 1 11
Unique Cell Count 226 151 138 190 173 292 350 222 258 167 248 323 501 640 225 57 143 170 473 464
Labelled Cell Count 250 164 163 198 201 345 386 283 318 205 324 323 503 641 225 57 143 170 473 464


Ambiguous, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 – 3.2 3.1 3.2 3.1 2.7 3.1 2.8 2.6 3.0 2.3 3.9 3.8 4.0 3.8 4.7 4.8 4.1 3.7 4.1
Std Deviation (1e-4) 0.4 – 1.1 1.3 1.0 1.7 1.3 1.6 1.5 1.3 1.8 0.8 1.0 1.1 1.3 1.5 2.6 1.3 2.1 1.4 1.4
Intensity Change (Log2) – – – -0.05 0.03 -0.03 -0.24 -0.02 -0.17 -0.27 -0.1 -0.46 0.31 0.25 0.32 0.28 0.56 0.59 0.37 0.22 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm -0.2 0.5 -2.7 -3.3 -2.4 -5.3 -4.0 -7.1 -5.2 3.7 3.0 5.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7208 0.0435 -0.2061 -0.1883 -0.5116 0.1454 -1.9359 -1.3982 -0.9832 -1.1764 -0.3251 -1.0191 -0.3276 0.0511 -0.2191 -0.4128 -0.1861 -0.3108
Actin 0.0072 0.0007 0.0006 0.0015 0 0.0008 0.029 0.0001 0.0103 0.0005 0.0429 0.0005 0.0351 0.0001 0.0117 0.0013 0.0043 0.0036
Bud 0.0004 0.0005 0.0003 0.0012 0 0.0025 0.0001 0 0.0007 0.0002 0.0032 0 0.0009 0.0022 0.0008 0.0046 0.0003 0.0005
Bud Neck 0.0008 0.0014 0.0001 0.0001 0 0.0004 0.0007 0 0.0004 0.0001 0.0003 0.0001 0.0032 0 0.0002 0.0069 0.0009 0.0004
Bud Periphery 0.0006 0.0004 0.0004 0.0017 0 0.001 0.0002 0 0.0015 0.0001 0.0089 0.0001 0.0022 0.0009 0.0009 0.0086 0.0006 0.0001
Bud Site 0.0063 0.0031 0.0006 0.0002 0 0.0008 0.0004 0 0.0043 0.0008 0.0023 0.0001 0.0022 0.0229 0.0052 0.0055 0.0035 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0.0009 0 0.0003 0 0.0003 0.0001 0.0001 0.0005 0.0001 0
Cytoplasm 0.0247 0.0445 0.0446 0.0977 0.012 0.0606 0.0072 0.0058 0.0121 0.0077 0.0142 0.0113 0.0268 0.0273 0.025 0.0399 0.0405 0.0549
Cytoplasmic Foci 0.0035 0.0063 0.0048 0.0033 0.0032 0.0067 0.0176 0.0012 0.0199 0.0427 0.0319 0.0029 0.0048 0.0021 0.0083 0.0063 0.0052 0.009
Eisosomes 0.0006 0.0004 0.001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0008 0.0006 0.0004 0.0003 0.0004 0.0001 0.0003 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0005 0.0009 0.0008 0.0011 0.0001 0.0006 0.0005 0.0002 0.0025 0.0003 0.0013 0.0001 0.0022 0.0002 0.0004 0.0005 0.0004 0.0002
Endosome 0.0001 0.0013 0.0006 0.0007 0.0001 0.0027 0.0017 0.0001 0.028 0.0878 0.0812 0.0046 0.0027 0.0002 0.0006 0.0017 0.0033 0.0003
Golgi 0.0001 0.0005 0.0001 0.0001 0 0.0003 0.0012 0 0.0081 0.0241 0.0331 0.0027 0.0013 0 0.0007 0.0002 0.0009 0.0004
Lipid Particles 0.0003 0.0009 0.0003 0.0001 0.0003 0.0004 0.0036 0.0004 0.0144 0.0011 0.0111 0.0123 0.0017 0.0001 0.0029 0.0002 0.0034 0.0013
Mitochondria 0.0002 0.0037 0.0003 0.0008 0 0.0004 0.0003 0 0.0189 0.0022 0.0324 0.0116 0.0007 0.0001 0.0004 0.0013 0.0008 0.0026
None 0.953 0.9272 0.9339 0.8818 0.9835 0.9154 0.9326 0.9919 0.8541 0.8258 0.7046 0.9498 0.8919 0.942 0.9294 0.9187 0.9192 0.9188
Nuclear Periphery 0.0002 0.0004 0.0004 0.0019 0.0001 0.0016 0.0002 0 0.0022 0.0001 0.0008 0.0002 0.0021 0.0001 0.0002 0.0007 0.0007 0.0002
Nucleolus 0.0002 0.0004 0.0005 0.0004 0 0.0007 0.0002 0 0.0003 0.0001 0.0003 0.0002 0.0011 0.0002 0.0003 0.0003 0.0025 0.0005
Nucleus 0.0003 0.0009 0.0009 0.0035 0.0001 0.0014 0.0001 0 0.001 0.0001 0.0006 0.0001 0.005 0.0003 0.0005 0.0009 0.0013 0.0012
Peroxisomes 0.0003 0.004 0.0002 0.0004 0 0.0001 0.002 0 0.0142 0.0026 0.0107 0.0015 0.0119 0 0.0104 0.0004 0.003 0.0027
Punctate Nuclear 0.0004 0.0016 0.009 0.0025 0.0002 0.0024 0.0013 0 0.0011 0.0003 0.0019 0.0007 0.0009 0.0007 0.0012 0.0007 0.0083 0.0029
Vacuole 0.0001 0.0007 0.0003 0.0007 0.0001 0.0006 0.0003 0.0001 0.0031 0.0015 0.0124 0.0005 0.0022 0.0002 0.0003 0.0006 0.0005 0.0001
Vacuole Periphery 0 0.0001 0 0.0002 0 0.0002 0.0001 0 0.0011 0.0012 0.0053 0.0005 0.0004 0 0 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.2092 8.1857 8.605 11.5859 8.9042 10.6771 10.6905 16.2928 14.458 13.6311
Translational Efficiency 0.7928 0.9661 0.9307 0.6554 1.0393 0.9518 0.9133 0.5531 0.6966 0.5901

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2477 1292 663 905 1044 1265 168 105 3521 2557 831 1010

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 596.82 615.33 717.24 749.25 646.96 658.54 847.69 822.72 611.69 636.71 743.61 756.89
Standard Deviation 75.76 92.64 77.73 93.88 75.78 75.96 98.71 146.44 79.15 87.51 97.65 103.10
Intensity Change Log 2 — 0.044064 0.265160 0.328151 — 0.025595 0.389860 0.346725 — 0.034487 0.331367 0.337842

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.021273 0.000163 0.000207 0.000843 0.000035 0.000647 0.000471 0.005942 0.014976 0.000403 0.000260 0.001373
Bud Neck 0.034902 0.028349 0.002774 0.005532 0.002079 0.030303 0.005232 0.005818 0.025170 0.029316 0.003271 0.005562
Bud Site 0.033449 0.007115 0.005313 0.038950 0.000865 0.012814 0.014272 0.107453* 0.023788 0.009934 0.007124 0.046072
Cell Periphery 0.006090 0.000135 0.000114 0.000226 0.000115 0.000170 0.000184 0.000485 0.004318 0.000152 0.000128 0.000253
Cytoplasm 0.468063* 0.386068* 0.265910* 0.438357* 0.588044* 0.374701* 0.394403* 0.368754* 0.503638* 0.380444* 0.291887* 0.431121*
Cytoplasmic Foci 0.098895 0.224383* 0.011142 0.033782 0.084511 0.247877* 0.022495 0.019867 0.094630 0.236006* 0.013437 0.032335
Eisosomes 0.004646 0.000127 0.000032 0.000070 0.000048 0.000150 0.000066 0.000110 0.003283 0.000139 0.000039 0.000074
Endoplasmic Reticulum 0.004612 0.000952 0.002531 0.002104 0.000420 0.000878 0.004660 0.007189 0.003369 0.000915 0.002961 0.002632
Endosome 0.004747 0.024524 0.001308 0.006287 0.001547 0.019893 0.004274 0.027869 0.003798 0.022233 0.001908 0.008530
Golgi 0.007621 0.003339 0.000061 0.007130 0.000305 0.005661 0.000325 0.012028 0.005452 0.004488 0.000115 0.007639
Lipid Particles 0.013609 0.012712 0.001125 0.002126 0.003216 0.009276 0.002347 0.001401 0.010527 0.011012 0.001372 0.002050
Mitochondria 0.009069 0.014298 0.000406 0.003444 0.001121 0.015789 0.003237 0.059039 0.006713 0.015036 0.000978 0.009224
Mitotic Spindle 0.000581 0.003459 0.004186 0.016565 0.000144 0.011350 0.013106 0.037298 0.000451 0.007363 0.005989 0.018721
None 0.011331 0.005819 0.006901 0.006320 0.009944 0.002652 0.004973 0.004061 0.010920 0.004252 0.006511 0.006085
Nuclear Periphery 0.000351 0.000879 0.001069 0.001444 0.000338 0.000634 0.001441 0.000947 0.000347 0.000758 0.001145 0.001392
Nuclear Periphery Foci 0.000345 0.000429 0.000669 0.001094 0.000292 0.000340 0.002944 0.001102 0.000329 0.000385 0.001129 0.001095
Nucleolus 0.001616 0.003587 0.000613 0.000524 0.000794 0.001962 0.000436 0.000307 0.001372 0.002783 0.000577 0.000501
Nucleus 0.235902* 0.206210* 0.674536* 0.341511* 0.292991* 0.197214* 0.420421* 0.224881* 0.252830* 0.201759* 0.623163* 0.329386*
Peroxisomes 0.020341 0.013368 0.000705 0.002196 0.000575 0.021836 0.003141 0.002817 0.014480 0.017557 0.001198 0.002261
Vacuole 0.021923 0.061339 0.020283 0.090583 0.012360 0.044153 0.101067 0.108618 0.019087 0.052837 0.036615 0.092457
Vacuole Periphery 0.000635 0.002745 0.000115 0.000915 0.000253 0.001700 0.000505 0.004014 0.000522 0.002228 0.000194 0.001237

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 10.61 10.57 10.37 -3.38 -2.72 -4.03 -3.08 -2.73 -2.02 -1.95 10.33 10.39 10.01 -2.38 -2.97
Bud Neck 2.46 16.44 14.12 9.96 -2.74 -13.53 -2.52 -2.25 11.70 0.60 -2.03 15.32 12.56 14.14 -2.45
Bud Site 11.48 10.20 2.13 -4.60 -4.78 -7.42 -2.38 -2.88 -2.14 -2.31 7.89 8.16 -0.73 -4.45 -5.23
Cell Periphery 7.49 7.51 7.44 -2.67 -3.40 -3.52 -3.64 -2.17 -1.24 -0.76 7.42 7.45 7.37 -1.81 -2.88
Cytoplasm 9.09 19.88 9.88 1.51 -8.73 18.56 12.92 10.19 2.77 0.64 17.49 24.55 14.94 1.97 -7.76
Cytoplasmic Foci -19.98 28.41 21.73 33.40 -7.18 -21.93 13.54 13.84 32.13 0.21 -29.22 31.93 24.73 45.83 -6.52
Eisosomes 8.89 9.07 8.99 9.12 -9.99 -14.25 -4.28 -5.73 2.48 -1.85 8.75 9.00 8.91 11.56 -8.05
Endoplasmic Reticulum 6.00 2.02 2.28 -10.76 0.41 -1.84 -6.54 -4.41 -4.08 0.09 5.54 -1.46 -0.45 -10.04 1.42
Endosome -11.83 7.00 0.47 11.63 -4.44 -11.72 -4.78 -1.79 1.68 -1.24 -15.80 4.87 -1.48 12.57 -3.72
Golgi 4.67 9.99 2.87 0.05 -2.32 -6.73 0.80 -1.45 0.63 -1.49 1.48 10.00 1.63 0.86 -2.56
Lipid Particles 0.57 10.20 9.49 11.64 -4.07 -6.11 0.64 1.48 8.23 0.96 -0.46 10.19 9.58 14.58 -2.66
Mitochondria -3.12 12.46 6.27 7.24 -1.58 -8.10 -0.65 -1.76 -0.37 -1.69 -6.55 11.16 2.10 6.27 -2.17
Mitotic Spindle -2.74 -2.07 -5.45 -4.15 -3.57 -5.61 -1.87 -1.90 -0.57 -0.89 -6.14 -2.76 -5.81 -3.00 -3.56
None 4.38 4.43 5.15 0.85 1.44 7.49 5.60 5.28 -1.29 0.18 7.81 5.77 5.95 -0.46 1.24
Nuclear Periphery -5.94 -10.74 -14.69 -10.37 -8.33 -5.13 -10.87 -4.51 -3.89 -0.77 -6.98 -14.93 -15.70 -12.35 -7.16
Nuclear Periphery Foci -1.23 -4.04 -6.28 -6.14 -3.40 -0.52 -4.80 -3.63 -3.53 2.13 -1.02 -6.20 -7.48 -7.43 -0.11
Nucleolus -4.55 3.13 3.57 8.82 1.91 -6.12 1.95 4.15 7.73 0.59 -5.19 3.41 3.98 11.33 1.80
Nucleus 3.51 -38.06 -12.40 -13.75 22.52 9.06 -6.62 -0.91 -4.21 3.59 8.10 -34.74 -10.92 -15.66 20.58
Peroxisomes 3.47 12.26 11.22 9.05 -1.05 -11.73 -0.84 -1.01 10.92 0.35 -2.15 11.56 10.45 13.26 -0.81
Vacuole -13.89 -2.64 -20.31 -14.22 -18.05 -13.93 -9.83 -8.99 -7.70 -2.62 -18.17 -8.70 -22.42 -17.27 -14.73
Vacuole Periphery -7.85 5.23 0.85 7.88 -2.69 -9.89 -0.69 -1.46 0.39 -1.35 -10.57 4.93 -0.37 7.85 -2.83
Standard name
Human Ortholog
Description Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
Localization
Cell Percentages cytoplasm (34%), nucleus (4%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sas4

Sas4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sas4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available