Standard name
Human Ortholog
Description Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.98 0.96 0.87 0.82 0.72 0.67 0.7 0.7 0.76 0.98 0.98 0.98 0.99 0.99 0.95 0.96 0.95 0.88 0.84 0.86 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0.32 0.33 0.41 0.36 0.45 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.08 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 1
Bud 0 1 0 0 1 3 5 11 1 7 0 5 1 0 0 0 0 1 0 0 1 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 7 0 4 1 1 1 5 1 5 2 0 1 8 2 2 0 0 0 0 0 0
Cytoplasm 182 170 186 158 254 287 323 354 178 264 185 278 115 114 115 139 154 139 182 171 170 116 163 157
Endoplasmic Reticulum 0 1 1 1 0 0 1 0 4 1 1 2 1 0 1 4 11 13 0 0 2 3 2 2
Endosome 0 0 0 1 3 0 0 3 2 2 0 3 0 0 0 0 0 1 1 0 2 0 3 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0
Mitochondria 4 3 3 4 9 68 125 164 107 135 120 126 0 0 0 2 0 1 1 1 3 4 4 1
Nucleus 0 2 0 1 1 1 2 10 4 6 7 4 0 1 1 1 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 2 0 0 1 0 4 0 4 2 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1 0
Vac/Vac Membrane 0 0 3 1 8 13 7 24 21 34 9 23 1 1 0 3 1 4 1 1 0 3 3 5
Unique Cell Count 186 173 193 162 265 329 396 490 264 375 265 366 117 116 117 141 155 146 191 181 193 139 189 174
Labelled Cell Count 189 177 197 173 276 377 465 571 318 458 325 449 120 117 118 157 169 163 191 181 193 139 189 174


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.0 5.7 5.8 5.7 4.7 4.7 5.1 4.9 4.9 4.7 4.7 5.7 5.7 6.4 6.0 6.0 6.0 5.0 5.9 6.0
Std Deviation (1e-4) 0.7 1.3 1.6 6.4 2.1 1.2 1.8 1.5 1.6 1.6 1.5 1.2 0.7 0.8 1.0 1.3 1.0 1.2 1.0 1.4 1.3
Intensity Change (Log2) – – – 0.01 0.0 -0.29 -0.27 -0.15 -0.23 -0.22 -0.27 -0.27 -0.0 0.01 0.17 0.08 0.07 0.07 -0.2 0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -0.3 -3.5 -4.9 -7.0 -7.6 -7.2 -7.2 -6.1 1.0 1.0 1.0 1.2 1.8 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.2 0 0 8.7 0 9.1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.5 0 2.0 3.0 3.4 1.2 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.44 0.2051 0.0572 -0.2395 -0.3823 0.1993 1.0527 1.6091 1.5053 1.407 1.1749 1.6452 0.3371 0.9976 1.1258 0.8254 0.4252 0.7945
Actin 0.0006 0.0005 0.0013 0.0007 0.0006 0.0022 0.0215 0.0085 0.0055 0.0154 0.0638 0.0196 0.005 0.0005 0.0104 0.0016 0 0.0008
Bud 0 0.0001 0.0006 0.0001 0 0.0001 0.0016 0.0165 0.0014 0.0003 0.0009 0.0057 0.0002 0.0003 0.0008 0.0059 0 0.0002
Bud Neck 0.0001 0.0001 0.0006 0.0006 0.0002 0.0006 0.0108 0.0009 0.0009 0.0026 0.0278 0.0034 0.0001 0.0001 0.001 0.0003 0.0001 0.0012
Bud Periphery 0 0.0001 0.0005 0.0001 0 0.0001 0.0015 0.0058 0.001 0.0004 0.0011 0.0037 0.0004 0.0007 0.0008 0.0056 0 0.0001
Bud Site 0.0002 0.0007 0.0024 0.0002 0.0001 0.0001 0.0052 0.0405 0.0022 0.0005 0.0007 0.0008 0.0011 0.0003 0.0054 0.0011 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0 0
Cytoplasm 0.406 0.5687 0.4995 0.5572 0.4804 0.5257 0.4457 0.5467 0.5736 0.4593 0.5097 0.6135 0.3506 0.5819 0.5928 0.6336 0.4247 0.6633
Cytoplasmic Foci 0.0195 0.0274 0.0259 0.0201 0.0147 0.013 0.0322 0.0083 0.0286 0.0106 0.0623 0.0346 0.0101 0.0042 0.0234 0.0049 0.0011 0.005
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0007 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0043 0.0022 0.0039 0.0028 0.0029 0.0021 0.0071 0.0015 0.0021 0.0026 0.0024 0.0034 0.0021 0.0017 0.0029 0.0025 0.0003 0.0025
Endosome 0.008 0.0061 0.0085 0.0099 0.007 0.0042 0.0147 0.002 0.0032 0.0077 0.0394 0.0093 0.0079 0.0008 0.0117 0.0039 0.0001 0.0014
Golgi 0.0004 0.0007 0.0004 0.0005 0.0003 0.0002 0.004 0.0003 0.001 0.0059 0.0088 0.0032 0.0036 0.0002 0.0079 0.001 0 0.0001
Lipid Particles 0.0008 0.0002 0.0006 0.0003 0.0004 0.0002 0.003 0.0002 0.0008 0.0037 0.0065 0.0023 0.0029 0.0001 0.0029 0.0001 0 0.0001
Mitochondria 0.0003 0.0003 0.0008 0.0005 0.0006 0.0003 0.0065 0.0004 0.0008 0.005 0.0023 0.002 0.0084 0.0002 0.0127 0.0088 0 0.0001
None 0.5534 0.3871 0.44 0.3953 0.4748 0.4432 0.4227 0.36 0.3654 0.4743 0.2486 0.276 0.593 0.4073 0.3075 0.3255 0.5733 0.3193
Nuclear Periphery 0.0012 0.0008 0.0019 0.0012 0.0019 0.0007 0.0044 0.0007 0.0009 0.0027 0.0042 0.0053 0.0021 0.0003 0.0027 0.0008 0.0001 0.0007
Nucleolus 0.0001 0.0001 0.0006 0.0001 0.0016 0.0003 0.0005 0.0004 0.0018 0.0004 0.0007 0.0005 0.0004 0 0.0001 0.0001 0 0
Nucleus 0.0013 0.0019 0.0028 0.0067 0.0048 0.0028 0.0049 0.0029 0.0021 0.0029 0.0027 0.003 0.0018 0.0005 0.0018 0.0014 0.0002 0.0033
Peroxisomes 0.0005 0.0009 0.0007 0.0005 0.0002 0.0008 0.0059 0.0003 0.0051 0.0007 0.0045 0.0049 0.0055 0.0001 0.0122 0.0002 0 0.0001
Punctate Nuclear 0.0016 0.0009 0.0074 0.0013 0.0075 0.0026 0.0053 0.0028 0.0024 0.0024 0.0102 0.0067 0.0027 0.0003 0.001 0.0003 0.0001 0.0011
Vacuole 0.001 0.001 0.0011 0.0014 0.001 0.0005 0.0016 0.0008 0.0008 0.0013 0.0022 0.001 0.0012 0.0004 0.0011 0.0017 0.0001 0.0004
Vacuole Periphery 0.0002 0.0001 0.0002 0.0003 0.0002 0.0001 0.0005 0.0001 0.0001 0.0006 0.0009 0.0005 0.0004 0 0.0003 0.0007 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.9027 11.3867 11.618 11.8013 12.3863 7.9567 12.5925 12.1415 10.1404 16.7607
Translational Efficiency 1.856 1.5403 1.5004 1.4285 1.372 4.4752 1.638 1.387 1.9846 1.4185

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1451 1768 641 1139 1537 1742 689 2129 2988 3510 1330 3268

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 698.59 775.40 855.75 897.40 671.80 758.08 919.25 873.06 684.81 766.80 888.65 881.54
Standard Deviation 79.57 109.15 96.74 150.02 105.03 98.10 98.74 114.21 94.49 104.17 102.80 128.36
Intensity Change Log 2 — 0.150495 0.292743 0.361305 — 0.174318 0.452425 0.378049 — 0.162223 0.373232 0.369538

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000112 0.000243 0.000133 0.001043 0.000108 0.000504 0.000129 0.000101 0.000110 0.000373 0.000131 0.000429
Bud Neck 0.013638 0.012872 0.001378 0.005947 0.009094 0.032695 0.001277 0.006473 0.011301 0.022710 0.001325 0.006290
Bud Site 0.003651 0.008949 0.001194 0.034968 0.004332 0.024328 0.003262 0.019072 0.004001 0.016582 0.002265 0.024612
Cell Periphery 0.000098 0.000090 0.000102 0.000556 0.000105 0.000144 0.000085 0.000065 0.000102 0.000117 0.000093 0.000236
Cytoplasm 0.556002* 0.603007* 0.411330* 0.513954* 0.538022* 0.471131* 0.552560* 0.727403* 0.546753* 0.537557* 0.484494* 0.653009*
Cytoplasmic Foci 0.201005* 0.154324 0.012467 0.032516 0.196865* 0.240196* 0.015843 0.012851 0.198875* 0.196942* 0.014216 0.019705
Eisosomes 0.000114 0.000064 0.000032 0.000049 0.000136 0.000161 0.000032 0.000014 0.000125 0.000112 0.000032 0.000026
Endoplasmic Reticulum 0.000611 0.000807 0.002146 0.001952 0.000918 0.000567 0.001716 0.000364 0.000769 0.000688 0.001923 0.000917
Endosome 0.014244 0.009938 0.001635 0.006184 0.011485 0.022428 0.002070 0.001532 0.012825 0.016137 0.001860 0.003153
Golgi 0.001882 0.002677 0.000052 0.004640 0.001828 0.008289 0.000049 0.000580 0.001855 0.005462 0.000050 0.001995
Lipid Particles 0.005758 0.003138 0.001249 0.002504 0.006990 0.005177 0.000984 0.000308 0.006392 0.004150 0.001112 0.001073
Mitochondria 0.001895 0.002749 0.000320 0.003745 0.001852 0.009919 0.000249 0.001251 0.001873 0.006307 0.000283 0.002120
Mitotic Spindle 0.000391 0.001258 0.001188 0.033674 0.000988 0.007544 0.000648 0.017448 0.000698 0.004378 0.000908 0.023104
None 0.002056 0.004941 0.005922 0.013191 0.007844 0.001558 0.004592 0.003876 0.005033 0.003262 0.005233 0.007123
Nuclear Periphery 0.000285 0.000292 0.001590 0.001228 0.000438 0.000276 0.001227 0.000565 0.000364 0.000284 0.001402 0.000796
Nuclear Periphery Foci 0.000310 0.000772 0.001129 0.002089 0.000712 0.000246 0.001401 0.000161 0.000517 0.000511 0.001270 0.000833
Nucleolus 0.000701 0.001348 0.000493 0.001340 0.002113 0.001662 0.000236 0.000248 0.001427 0.001504 0.000360 0.000629
Nucleus 0.118060 0.121868 0.506125* 0.199866* 0.146297* 0.074301 0.338768* 0.149540* 0.132585* 0.098261 0.419426* 0.167080*
Peroxisomes 0.003522 0.005677 0.000144 0.000980 0.003390 0.013517 0.000114 0.000280 0.003454 0.009568 0.000128 0.000524
Vacuole 0.074906 0.064034 0.051272 0.135302 0.065688 0.082839 0.074669 0.057490 0.070164 0.073367 0.063393 0.084610
Vacuole Periphery 0.000758 0.000951 0.000099 0.004273 0.000795 0.002517 0.000089 0.000379 0.000777 0.001728 0.000094 0.001736

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.94 -4.43 -4.75 -3.72 -3.81 -4.42 -3.42 0.50 4.54 4.33 -5.68 -5.60 -4.48 0.73 -2.47
Bud Neck 0.49 10.27 7.17 7.08 -6.72 -12.09 8.78 3.27 14.37 -6.39 -9.30 13.48 7.16 15.45 -8.23
Bud Site -6.53 4.88 -8.19 -5.74 -8.92 -9.63 0.89 -5.86 4.52 -5.64 -11.25 2.02 -9.97 -0.17 -10.36
Cell Periphery 1.24 -1.21 -9.17 -9.47 -8.45 -2.27 2.86 9.91 5.28 5.85 -1.66 0.34 -6.12 -4.25 -5.93
Cytoplasm -4.76 16.79 13.65 18.15 -4.57 6.76 6.56 -13.59 -20.65 -17.22 1.48 16.07 -3.45 -4.86 -18.43
Cytoplasmic Foci 7.09 36.70 33.85 29.22 -6.43 -5.89 36.49 37.58 42.65 0.78 0.48 51.75 51.30 51.28 -3.45
Eisosomes 9.06 9.63 11.68 2.24 -1.38 -0.82 3.96 5.04 8.08 10.23 0.88 6.35 7.66 9.03 4.99
Endoplasmic Reticulum -1.92 -11.83 -13.02 -11.12 4.81 3.49 -8.95 4.14 0.68 12.44 1.02 -14.77 -5.52 -7.21 12.39
Endosome 3.07 10.46 8.85 7.42 -2.75 -7.77 12.21 13.44 18.34 3.75 -3.30 15.61 15.53 19.65 0.79
Golgi -1.96 8.66 0.41 1.52 -2.52 -7.75 5.14 3.89 10.28 -1.77 -7.72 8.76 3.38 9.66 -3.04
Lipid Particles 4.37 7.88 5.91 2.71 -0.50 2.62 10.34 11.78 10.74 8.22 4.82 12.97 13.52 10.75 2.39
Mitochondria -1.50 3.82 0.20 1.52 -2.74 -7.41 4.61 2.11 8.38 -2.40 -7.20 5.93 1.80 8.30 -3.59
Mitotic Spindle -2.48 -3.23 -8.23 -7.89 -7.26 -4.13 -1.42 -7.03 -3.03 -6.24 -4.53 -3.00 -10.80 -7.86 -9.44
None -4.02 -4.45 -4.00 -1.61 -1.57 4.95 2.15 2.97 -8.85 1.71 2.37 0.06 -0.13 -3.33 -0.26
Nuclear Periphery -0.23 -13.63 -13.59 -13.69 1.68 3.28 -16.08 -8.47 -10.71 8.92 2.31 -19.77 -16.04 -17.40 7.32
Nuclear Periphery Foci -7.50 -4.87 -9.98 -6.73 -0.67 7.18 -5.76 7.90 1.32 9.05 -0.13 -7.22 -4.61 -4.53 5.12
Nucleolus -3.28 2.35 -0.06 2.21 -0.98 1.11 5.95 5.85 4.52 -0.88 -0.26 6.35 5.52 5.47 -0.81
Nucleus -0.92 -33.27 -13.13 -12.50 23.43 14.85 -21.35 -7.16 -23.71 17.49 10.02 -37.71 -14.51 -24.14 29.06
Peroxisomes -2.61 9.27 7.97 6.54 -2.49 -8.97 5.25 5.04 14.40 -1.20 -8.56 9.02 8.42 15.09 -2.34
Vacuole 2.27 -3.43 -22.36 -23.35 -16.37 -4.33 -8.86 -8.29 -4.16 3.90 -1.56 -8.88 -20.83 -19.65 -7.33
Vacuole Periphery -1.69 11.29 -1.05 -0.78 -1.94 -6.20 12.77 6.57 8.11 -2.55 -6.35 17.02 0.32 3.51 -2.31
Standard name
Human Ortholog
Description Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Plp1

Plp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Plp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available