Standard name
Description Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.82 0.88 0.7 0.55 0.29 0.27 0.28 0.18 0.2 0.15 0.1 0.69 0.78 0.83 0.86 0.76 0.21 0.36 0.33 0.23 0.3 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0.08 0.2 0 0.13 0.11 0 0.12 0 0.09 0 0.12 0.1 0 0.05 0.12 0.06 0 0.06 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.16 0 0.17 0.11 0.71 0.41 0.56 0.78 0.66 0.79 0.76 0.15 0.05 0 0.05 0.13 0.49 0.39 0.48 0.48 0.42 0.35
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.06 0 0.19 0.11 0.08 0.09 0.09 0.13 0 0 0 0 0 0 0 0.05 0 0.07 0
Vac/Vac Membrane 0.11 0 0 0 0.11 0 0.13 0.08 0.05 0.12 0.06 0 0 0.08 0.06 0.07 0 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 0 1 0 0 0 1 0 0 0 0 4 0 4 0 1 7 10 1 8 13 10
Bud 2 3 0 1 2 11 2 8 13 13 19 18 2 1 3 1 1 2 0 1 3 4 10
Bud Neck 1 1 4 0 0 0 0 4 2 4 4 4 2 0 0 1 2 4 4 1 3 6 10
Bud Site 0 0 0 0 0 0 0 2 4 0 6 4 0 0 0 0 0
Cell Periphery 1 1 0 2 1 0 5 0 2 3 3 1 2 2 6 6 7 0 4 1 1 1 4
Cytoplasm 162 177 84 105 132 69 94 110 66 73 49 36 157 288 321 170 148 65 102 48 54 107 133
Endoplasmic Reticulum 4 3 1 0 0 0 1 0 1 0 1 1 24 0 3 10 12 5 1 2 2 3 4
Endosome 8 1 1 12 48 5 46 44 15 44 9 31 9 43 37 7 10 36 17 5 13 20 19
Golgi 3 0 1 0 1 0 0 1 0 0 1 0 0 0 0 0 0 4 0 0 1 0 0
Mitochondria 59 35 3 25 26 170 144 222 290 239 258 264 35 19 6 10 25 154 110 70 112 152 126
Nucleus 0 0 0 0 1 11 11 16 11 3 8 7 0 0 1 3 2 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 0 0 2 0 0 2 1 1
Nucleolus 0 0 0 0 0 4 1 0 2 4 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 7 21 0 10 3 1 15 6 15 0 18 8 3 4 0 0 1 2 1 0
SpindlePole 4 1 5 5 14 9 65 43 29 32 30 44 4 2 7 2 7 11 6 6 10 24 14
Vac/Vac Membrane 26 6 2 6 26 8 47 33 20 43 18 13 6 29 22 13 4 8 16 2 9 9 20
Unique Cell Count 241 215 95 149 239 238 350 397 373 361 326 347 229 368 387 198 194 315 284 148 235 359 365
Labelled Cell Count 275 231 101 164 272 287 428 487 456 475 414 438 245 402 418 226 223 315 284 148 235 359 365


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.2 5.1 5.8 5.9 4.1 5.2 4.9 4.3 4.5 4.2 4.4 4.8 5.2 5.3 4.7 5.5 4.9 4.4 4.5 4.5
Std Deviation (1e-4) 0.6 0.7 1.6 7.6 2.1 1.8 2.2 1.8 1.5 1.5 1.5 1.2 0.7 1.5 1.3 0.8 1.3 1.0 1.3 1.4 1.2
Intensity Change (Log2) 0.2 0.21 -0.31 0.04 -0.06 -0.25 -0.16 -0.26 -0.21 -0.08 0.02 0.05 -0.12 0.11 -0.05 -0.2 -0.19 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ups3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available