Standard name
Human Ortholog
Description Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.08 0.07 0.09 0 0 0 0 0 0.07
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0.12 0.47 0.24 0.35 0.05 0 – 0 0 0 0 0.3 0.45 0.39 0.65 0.45 0.32 0.06 0.11 0.09 0.15 0.23 0.18
Mitochondria 0.94 0.86 0.71 0.85 0.78 0.9 0.8 – 0.88 0.85 0.92 0.88 0.89 0.87 0.74 0.1 0.15 0.08 0.84 0.78 0.77 0.73 0.64 0.61
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 – 0.06 0.06 0.05 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0.1 0 0.06 0.05 0.1 0.08 0.11 – 0.1 0.08 0.11 0.08 0 0 0 0.06 0.1 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0.05 – 0 0 0 0 0 0 0.07 0.05 0 0.06 0 0 0.06 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.15 0.18 0 0.06 0 0 – 0.06 0 0 0 0.1 0.05 0.06 0.09 0.21 0.19 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 2 3 10 1 3 – 6 2 4 3 4 13 4 14 11 9 1 6 2 3 10 22
Bud 0 1 0 4 3 5 10 – 7 8 9 10 1 3 4 0 5 5 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 – 1 0 3 1 0 0 0 0 0 1 – – – – – –
Cell Periphery 2 7 2 5 1 1 4 – 1 3 0 4 4 6 9 8 9 9 0 0 0 0 0 0
Cytoplasm 1 0 2 2 4 4 14 – 0 7 2 7 2 1 8 7 4 7 0 0 0 0 1 1
Endoplasmic Reticulum 2 0 0 0 0 1 0 – 0 2 1 1 0 1 1 4 2 0 0 0 0 0 0 1
Endosome 0 0 3 0 3 2 3 – 2 7 1 10 3 4 3 6 1 5 1 1 6 1 3 7
Golgi 2 21 72 49 95 13 15 – 11 14 7 10 55 128 112 111 74 31 11 20 15 21 66 58
Mitochondria 194 145 109 171 211 240 307 – 259 293 302 355 161 246 212 17 24 8 176 144 133 100 186 200
Nucleus 1 1 0 0 0 1 0 – 1 2 0 1 0 0 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 9 3 1 6 3 11 25 – 19 20 15 19 3 4 7 3 10 3 0 0 0 0 1 3
Nucleolus 21 4 9 10 27 22 41 – 30 28 35 31 4 6 9 11 16 13 1 0 0 0 1 2
Peroxisomes 4 1 3 6 13 10 20 – 7 5 6 15 2 10 19 8 5 6 7 2 10 3 5 4
SpindlePole 0 1 1 0 2 1 2 – 1 3 4 3 0 0 0 2 0 2 2 0 0 1 1 0
Vac/Vac Membrane 9 25 27 9 17 8 13 – 17 13 8 16 19 15 18 16 35 18 1 5 2 5 10 19
Unique Cell Count 207 168 153 200 271 267 383 293 343 327 404 181 283 285 170 163 96 209 185 175 138 292 327
Labelled Cell Count 245 212 231 265 389 320 458 362 407 397 486 258 437 406 208 197 118 209 185 175 138 292 327


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 22.7 16.3 17.4 18.3 17.1 15.7 – 13.9 15.3 13.0 13.4 18.5 18.5 17.8 22.1 22.8 21.1 16.7 18.4 19.4
Std Deviation (1e-4) 3.7 4.6 3.3 4.3 4.1 5.3 4.0 – 4.0 4.7 3.7 3.8 3.8 3.6 3.7 6.4 6.9 6.3 3.4 4.2 4.6
Intensity Change (Log2) – – – 0.1 0.17 0.07 -0.05 – -0.23 -0.09 -0.32 -0.28 0.18 0.18 0.13 0.44 0.49 0.37 0.04 0.18 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 1.8 0 2.9 2.4 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 2.0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -4.4 -2.4 -10.4 -12.2 – -11.2 -11.7 -12.4 -13.3 -3.1 -0.4 -1.6 3.3 -0.3 -2.3
Mitochondria 3.3 1.5 4.9 2.2 – 4.5 3.7 6.1 4.7 4.1 4.0 0.7 -11.3 -10.2 -9.7
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2.9 – 2.8 2.6 2.2 2.3 0 0 0 0 2.7 0
Nucleolus -0.4 1.4 0.9 1.7 – 1.5 0.9 1.7 0.7 -1.7 -2.1 -1.4 0.2 1.3 2.1
Peroxisomes 0 1.5 0 1.7 – 0 0 0 0 0 0 2.1 1.4 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -3.7 -5.2 -5.7 – -4.0 -5.2 -6.0 -5.4 -1.9 -4.2 -3.7 -2.2 0.9 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.4332 19.8308 19.9968 18.2544 16.9516 19.0451 23.6214 23.3738 22.2494 22.7208 18.3989 22.974 21.4635 23.1207 22.952 21.1477 16.9689 21.8111
Actin 0.0244 0.0034 0.011 0.0004 0.0201 0.0009 0.0105 0.005 0.0089 0.0047 0.0323 0.0009 0.0177 0.0088 0.0089 0.0002 0.0174 0.0005
Bud 0.0003 0.0002 0.0001 0 0.0003 0 0.0001 0.0002 0.0001 0 0.0016 0 0.0001 0.0001 0.0002 0 0.0045 0
Bud Neck 0.001 0.0001 0.0005 0 0.0001 0 0.0006 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0003 0 0.0003 0
Bud Periphery 0.0005 0.0006 0.0002 0 0.0004 0 0.0002 0.0004 0.0001 0 0.0035 0 0.0001 0.0002 0.0003 0 0.0196 0
Bud Site 0.0034 0.0002 0.0011 0 0.0008 0 0.0005 0.0004 0.0002 0 0.0002 0 0.0014 0.0003 0.0048 0 0.0045 0.0002
Cell Periphery 0.0003 0.0003 0.0001 0 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0005 0.0002 0 0.0005 0.0001
Cytoplasm 0.0023 0 0.0037 0 0.0003 0 0.0002 0 0.0001 0 0.0002 0 0.0025 0 0.0006 0 0.0003 0.0002
Cytoplasmic Foci 0.0142 0.0007 0.0033 0.0001 0.0068 0.0013 0.0055 0.0004 0.0021 0.0002 0.0068 0 0.0194 0.0003 0.0007 0 0.0022 0.0105
Eisosomes 0.0009 0.0003 0.0017 0.0002 0.0007 0.0002 0.001 0.0006 0.0013 0.0008 0.0004 0.0005 0.0009 0.0005 0.0004 0.0001 0.0007 0.0001
Endoplasmic Reticulum 0.0017 0.0001 0.0006 0 0.0005 0 0.0004 0.0002 0.0005 0 0 0 0.0007 0 0.0023 0 0.0008 0.0001
Endosome 0.0172 0.0011 0.0047 0.0002 0.0052 0.0008 0.0044 0.0023 0.0028 0.0001 0.0102 0.0003 0.0054 0.0009 0.0012 0 0.0072 0.0026
Golgi 0.0159 0.0037 0.006 0.0011 0.02 0.0083 0.0098 0.0093 0.0053 0.0006 0.0115 0.0103 0.0094 0.0132 0.0021 0.0002 0.0053 0.0036
Lipid Particles 0.0577 0.0198 0.0143 0.001 0.0237 0.0173 0.0203 0.0014 0.0079 0.0113 0.0143 0.0003 0.0235 0.0103 0.0072 0.0004 0.0139 0.0098
Mitochondria 0.8331 0.9514 0.9299 0.9944 0.9043 0.9633 0.9228 0.9709 0.9456 0.9733 0.9068 0.986 0.8817 0.9505 0.9627 0.9979 0.9025 0.955
None 0.0007 0 0.0001 0 0.0011 0 0.0008 0 0.0001 0 0 0 0.0053 0 0.0005 0 0.0009 0.0004
Nuclear Periphery 0.0015 0.0001 0.002 0 0.0017 0 0.0029 0.0003 0.0002 0 0 0 0.0028 0 0.0009 0 0.0028 0.0023
Nucleolus 0.0011 0.0004 0.0002 0 0.0005 0.0012 0.0056 0.0002 0.0001 0.0002 0.0001 0 0.0057 0.0002 0.0001 0 0.0016 0.0004
Nucleus 0.0011 0.0002 0.0006 0 0.0004 0.0001 0.0013 0.0002 0 0 0 0 0.0011 0.0001 0.0002 0 0.0008 0.0003
Peroxisomes 0.0149 0.0062 0.0075 0.002 0.0077 0.0021 0.0077 0.0024 0.017 0.0053 0.0101 0.001 0.0094 0.0022 0.002 0.0007 0.0051 0.0024
Punctate Nuclear 0.0016 0 0.0005 0 0.0031 0.0001 0.0017 0 0.0001 0 0.0001 0 0.0043 0 0.0003 0 0.001 0.0005
Vacuole 0.002 0.0034 0.0015 0.0001 0.0004 0.0002 0.0007 0.0025 0.0011 0.0002 0.0004 0 0.001 0.0015 0.0004 0 0.0028 0.0004
Vacuole Periphery 0.0041 0.0077 0.0103 0.0004 0.0018 0.0042 0.0029 0.003 0.0064 0.0032 0.0013 0.0006 0.0071 0.0102 0.0036 0.0004 0.0054 0.0106

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 79.0911 62.2974 66.406 70.9322 58.085 64.1223 81.7356 74.9043 69.3046 86.744
Translational Efficiency 0.712 0.8838 0.7479 0.6519 0.7768 0.7957 0.7588 0.6863 0.7752 0.7088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1965 891 138 1454 1905 2062 1498 161 3870 2953 1636 1615

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1122.88 1504.57 2371.19 1943.52 1548.61 1536.62 1880.03 2258.89 1332.44 1526.95 1921.46 1974.96
Standard Deviation 207.23 381.24 409.41 362.32 338.66 351.56 341.10 497.80 351.51 361.07 373.24 389.64
Intensity Change Log 2 — 0.422147 1.078408 0.791468 — -0.011213 0.279782 0.544640 — 0.186991 0.670232 0.653572

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002526 0.013765 0.016345 0.012762 0.002398 0.007905 0.006458 0.020010 0.002463 0.009673 0.007292 0.013485
Bud Neck 0.000976 0.002281 0.008888 0.001606 0.000262 0.000718 0.002842 0.001864 0.000625 0.001190 0.003352 0.001632
Bud Site 0.001835 0.001626 0.003016 0.004001 0.000613 0.000829 0.002048 0.002090 0.001234 0.001070 0.002130 0.003810
Cell Periphery 0.000191 0.000105 0.000136 0.000135 0.000045 0.000071 0.000071 0.000164 0.000119 0.000081 0.000077 0.000138
Cytoplasm 0.000623 0.000439 0.000503 0.000415 0.000131 0.000269 0.000474 0.000317 0.000381 0.000321 0.000477 0.000405
Cytoplasmic Foci 0.015465 0.031280 0.084355 0.009314 0.006089 0.012545 0.033373 0.018567 0.010849 0.018198 0.037673 0.010237
Eisosomes 0.000256 0.002937 0.001404 0.001449 0.001682 0.001671 0.000832 0.001506 0.000958 0.002053 0.000880 0.001455
Endoplasmic Reticulum 0.000147 0.000217 0.000256 0.000117 0.000029 0.000090 0.000108 0.000251 0.000089 0.000128 0.000120 0.000130
Endosome 0.009309 0.002980 0.002578 0.001362 0.000361 0.001434 0.003102 0.003166 0.004905 0.001900 0.003057 0.001542
Golgi 0.025506 0.035577 0.036630 0.023831 0.008253 0.029040 0.034712 0.038099 0.017013 0.031012 0.034874 0.025254
Lipid Particles 0.009789 0.012794 0.013691 0.003380 0.003030 0.006813 0.012236 0.004275 0.006462 0.008618 0.012358 0.003469
Mitochondria 0.903476* 0.850140* 0.732032* 0.921474* 0.963329* 0.921146* 0.865123* 0.876245* 0.932939* 0.899721* 0.853896* 0.916965*
Mitotic Spindle 0.000800 0.000713 0.018929 0.001600 0.000068 0.000296 0.004805 0.001655 0.000440 0.000422 0.005996 0.001605
None 0.001276 0.003169 0.004397 0.001510 0.002445 0.001968 0.002438 0.003137 0.001851 0.002330 0.002603 0.001673
Nuclear Periphery 0.000308 0.000576 0.006945 0.000204 0.000079 0.000137 0.001504 0.000524 0.000195 0.000269 0.001963 0.000236
Nuclear Periphery Foci 0.000386 0.000322 0.001912 0.000146 0.000041 0.000083 0.000590 0.000231 0.000216 0.000155 0.000702 0.000155
Nucleolus 0.000886 0.001095 0.002960 0.000224 0.000109 0.000147 0.000909 0.000578 0.000503 0.000433 0.001082 0.000260
Nucleus 0.000094 0.000157 0.000169 0.000037 0.000028 0.000024 0.000098 0.000049 0.000062 0.000065 0.000104 0.000039
Peroxisomes 0.012455 0.030879 0.033190 0.011704 0.009441 0.010871 0.013591 0.014171 0.010972 0.016908 0.015244 0.011950
Vacuole 0.007452 0.003235 0.012156 0.001579 0.000510 0.001497 0.006110 0.005130 0.004035 0.002021 0.006620 0.001933
Vacuole Periphery 0.006244 0.005713 0.019506 0.003147 0.001057 0.002447 0.008578 0.007968 0.003690 0.003433 0.009500 0.003628

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.29 -2.72 -6.84 0.39 0.67 -4.59 -3.62 -2.66 -1.81 -2.03 -6.99 -4.61 -7.36 -2.17 -3.48
Bud Neck -3.17 -6.13 -2.10 1.25 5.48 -3.16 -7.91 -4.20 -2.94 2.04 -3.42 -8.48 -3.76 -1.63 4.06
Bud Site 0.27 -1.39 -1.96 -2.01 -0.93 -0.61 -2.90 -1.08 -0.90 0.03 0.35 -2.05 -2.58 -2.70 -1.64
Cell Periphery 2.21 1.33 1.21 -1.21 -0.07 -1.06 -1.52 -2.72 -2.10 -2.24 1.58 1.97 -0.73 -2.34 -2.78
Cytoplasm 0.92 0.55 0.93 0.10 0.60 -1.66 -3.93 -2.52 -0.30 1.57 0.43 -0.87 -0.31 -0.96 0.70
Cytoplasmic Foci -5.51 -4.87 4.18 7.71 5.30 -7.04 -12.68 -2.86 -1.36 3.12 -6.41 -11.40 0.43 5.70 11.05
Eisosomes -9.18 -5.70 -11.31 4.81 -0.19 0.05 4.24 0.36 0.35 -1.48 -6.72 0.71 -3.51 3.57 -4.88
Endoplasmic Reticulum -1.85 -2.45 1.40 2.63 3.12 -4.49 -6.40 -2.62 -1.89 -1.68 -2.51 -2.46 -2.27 -0.12 -0.54
Endosome 5.96 6.37 8.04 3.24 2.49 -5.05 -7.11 -1.97 -1.20 -0.04 5.64 3.04 6.09 1.14 3.58
Golgi -2.77 -1.88 0.58 3.13 2.13 -9.51 -10.62 -3.76 -1.11 -0.41 -7.00 -7.31 -3.50 2.15 3.17
Lipid Particles -1.59 -1.05 5.52 5.36 2.83 -4.15 -7.15 -0.79 1.48 4.10 -2.34 -4.64 3.72 5.37 6.85
Mitochondria 5.50 6.41 -2.57 -7.22 -7.05 9.20 15.03 4.87 2.47 -0.62 7.16 11.96 3.06 -2.76 -8.18
Mitotic Spindle 0.24 -3.18 -1.70 -2.05 3.02 -2.91 -5.34 -2.90 -2.46 3.04 0.10 -5.80 -3.02 -3.22 4.26
None -7.64 -5.34 -2.38 6.60 4.93 1.35 0.05 -1.03 -2.03 -1.22 -2.35 -3.70 0.94 4.84 6.82
Nuclear Periphery -1.72 -2.96 1.65 2.42 3.01 -2.98 -6.07 -3.53 -3.05 3.68 -1.41 -6.11 -1.02 0.53 5.94
Nuclear Periphery Foci 0.75 -3.56 2.65 1.96 4.10 -2.89 -6.69 -3.10 -2.38 3.52 1.62 -5.44 0.86 -0.08 5.36
Nucleolus -0.35 -1.82 1.90 1.74 2.51 -1.38 -6.32 -2.78 -2.56 1.61 0.30 -2.56 1.32 1.07 5.29
Nucleus -1.18 -2.40 2.76 2.33 5.03 0.22 -2.79 -0.90 -2.16 2.61 -0.14 -2.16 1.49 1.50 4.14
Peroxisomes -4.75 -2.50 0.37 4.90 2.58 -0.85 -2.19 -0.94 -0.65 -0.10 -3.56 -2.37 -0.60 2.49 1.57
Vacuole 4.07 -2.21 5.92 2.74 5.40 -4.07 -8.83 -2.33 -1.82 0.48 3.88 -3.41 3.42 0.14 6.61
Vacuole Periphery 0.43 -3.88 4.61 2.17 4.81 -4.08 -10.51 -3.67 -2.90 0.31 0.53 -7.67 0.11 -0.35 7.14
Standard name
Human Ortholog
Description Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mss116

Mss116


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mss116-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available