Standard name
Human Ortholog
Description Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0
Bud – – 0.06 0 0.06 0 0.06 0.06 0.06 0.07 0.08 0.06 0 0 0 0 0.05 0 – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm – – 0.34 0.38 0.4 0.23 0.19 0.14 0.11 0.09 0.09 0.08 0.92 0.94 0.94 0.53 0.54 0.5 – – 0 0.17 0.19 0.15
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – 0 0 0 0
Endosome – – 0.1 0 0.06 0 0 0 0 0 0 0 0 0 0 0.21 0.15 0.14 – – 0.07 0 0.07 0.05
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria – – 0.43 0.6 0.57 0.84 0.84 0.84 0.89 0.93 0.92 0.94 0 0 0 0.07 0.12 0.1 – – 0.68 0.56 0.53 0.56
Nucleus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Peroxisomes – – 0.12 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 – – 0 0 0 0
SpindlePole – – 0.1 0 0.06 0.05 0.07 0.11 0.1 0.09 0.07 0.1 0 0 0 0.16 0.13 0.17 – – 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.12 – – 0 0.07 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 2 0 0 0 0 0 0 0 0 0 5 0 0 2 2 0 – – 13 3 6 9
Bud – – 12 6 13 13 29 22 19 30 27 25 3 0 0 5 7 5 – – 0 1 1 4
Bud Neck – – 2 0 0 0 2 1 1 2 0 0 3 1 1 3 1 0 – – 2 3 1 7
Bud Site – – 0 2 0 3 6 12 6 18 14 19 0 0 0 0 1 0 – – – – – –
Cell Periphery – – 3 1 0 0 1 1 0 0 0 3 3 0 0 2 2 3 – – 1 3 0 1
Cytoplasm – – 68 59 91 81 86 55 39 38 32 33 261 234 244 78 76 78 – – 7 33 40 40
Endoplasmic Reticulum – – 7 5 2 3 2 1 0 0 3 0 2 3 1 8 5 6 – – 6 1 1 5
Endosome – – 19 1 13 1 1 5 0 1 0 0 4 4 7 31 21 22 – – 20 6 14 14
Golgi – – 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 – – 2 0 0 1
Mitochondria – – 87 93 129 292 385 337 305 380 329 368 1 0 3 10 17 16 – – 191 110 110 151
Nucleus – – 0 1 2 3 3 4 6 8 4 8 1 0 0 0 2 1 – – 0 2 0 1
Nuclear Periphery – – 1 0 0 1 0 0 2 1 3 6 0 0 0 0 0 1 – – 1 0 0 0
Nucleolus – – 0 0 0 2 0 1 0 3 0 2 0 0 0 0 1 0 – – 2 0 1 0
Peroxisomes – – 23 2 14 4 5 4 2 8 3 4 0 0 0 8 8 13 – – 8 0 1 3
SpindlePole – – 19 6 14 16 30 43 33 38 26 40 3 2 0 23 18 27 – – 12 6 5 8
Vac/Vac Membrane – – 6 2 6 6 17 8 5 14 5 7 6 8 9 11 9 19 – – 1 14 14 11
Unique Cell Count 200 155 226 349 458 400 341 407 356 393 283 248 260 148 142 157 282 199 208 268
Labelled Cell Count 250 178 284 425 567 494 418 541 446 515 293 252 265 182 171 194 282 199 208 268


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 3.9 3.6 4.0 3.0 3.0 3.1 2.8 2.8 2.5 2.5 5.0 5.2 4.9 5.7 5.6 5.7 5.0 5.8 5.8
Std Deviation (1e-4) – – 1.4 1.4 2.1 1.4 1.3 1.7 1.0 0.9 0.8 0.4 1.0 1.4 1.2 1.7 1.9 2.2 1.1 1.9 1.4
Intensity Change (Log2) – – – -0.11 0.06 -0.34 -0.37 -0.34 -0.47 -0.46 -0.65 -0.64 0.38 0.42 0.33 0.55 0.53 0.57 0.36 0.58 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -3.1 -3.9 0 -1.1 -0.4 -1.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 1.3 -2.7 -4.2 -5.8 -6.4 -7.5 -7.4 -7.8 13.5 13.5 13.6 3.5 3.6 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0 0.2
Endosome 0 0 0 0 0 0 0 0 0 -4.1 -3.8 0 3.0 1.5 1.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -12.1 -11.6 0 -7.6 -6.2 -6.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -5.8 -5.5 0 -2.0 -1.9 -1.0
SpindlePole 0 0 0 0 0 0 0 0 0 -4.4 -4.3 0 1.7 0.9 2.2
Vacuole 0 -0.2 0 0.5 0 0 0.3 0 0 0 0.1 0.3 1.9 1.5 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.066 0.0772 -0.1378 0.1787 -0.8721 0.0237 0.3963 0.0635 0.3581 0.7388 0.2361 0.2597 -0.4383 -0.4772 -0.9018 -0.4858 -0.5719 -0.8964
Actin 0.0311 0.0078 0.0049 0.0342 0.0019 0.0087 0.0292 0.0055 0.0124 0.0314 0.0509 0.0051 0.0441 0.0345 0.0319 0.0717 0.0234 0.0053
Bud 0.0013 0.0072 0.008 0.0022 0.0003 0.0009 0.0013 0.004 0.0032 0.0071 0.0083 0.0015 0.0011 0.0015 0.0071 0.0011 0.0041 0.001
Bud Neck 0.008 0.0013 0.0059 0.0005 0.0042 0.0083 0.0018 0.0028 0.005 0.0015 0.0043 0.0208 0.0013 0.0011 0.0022 0.0007 0.0017 0.0133
Bud Periphery 0.0018 0.0067 0.0088 0.0088 0.0009 0.0026 0.0025 0.0091 0.0066 0.0348 0.0189 0.0039 0.0014 0.0025 0.0087 0.0027 0.0113 0.0024
Bud Site 0.0147 0.0335 0.0128 0.0032 0.0009 0.001 0.0077 0.0208 0.022 0.0077 0.0132 0.0014 0.0043 0.0123 0.0124 0.002 0.0081 0.0009
Cell Periphery 0.002 0.002 0.0018 0.0006 0.0003 0.0007 0.0007 0.0015 0.0014 0.001 0.0021 0.0005 0.0007 0.0017 0.001 0.0005 0.001 0.0005
Cytoplasm 0.0512 0.0589 0.0519 0.0288 0.0329 0.0679 0.0649 0.1538 0.0761 0.1437 0.077 0.1498 0.0582 0.1505 0.0866 0.0289 0.1027 0.1231
Cytoplasmic Foci 0.151 0.1897 0.1285 0.1644 0.1661 0.1604 0.1367 0.146 0.142 0.1304 0.0876 0.1658 0.1726 0.1431 0.1589 0.1723 0.1357 0.1704
Eisosomes 0.0007 0.0014 0.0019 0.0013 0.0002 0.0005 0.0004 0.0004 0.0006 0.0003 0.0015 0.0002 0.0006 0.0018 0.001 0.0005 0.0004 0.0002
Endoplasmic Reticulum 0.0034 0.0039 0.002 0.0037 0.0013 0.003 0.0147 0.0068 0.0058 0.0036 0.0158 0.007 0.0149 0.0066 0.0068 0.002 0.011 0.0087
Endosome 0.1567 0.121 0.0976 0.079 0.0794 0.1177 0.242 0.1859 0.1548 0.1688 0.2396 0.1824 0.1451 0.1742 0.101 0.2303 0.1593 0.1774
Golgi 0.0391 0.0492 0.0351 0.0448 0.0902 0.0402 0.0455 0.0369 0.0315 0.0226 0.0474 0.0426 0.0383 0.0332 0.0322 0.0571 0.0353 0.0636
Lipid Particles 0.0665 0.0325 0.0341 0.0336 0.009 0.0487 0.0487 0.0116 0.0308 0.0265 0.0244 0.0278 0.082 0.031 0.0333 0.0233 0.0382 0.0274
Mitochondria 0.1618 0.157 0.29 0.2296 0.3355 0.1735 0.1148 0.1319 0.2132 0.0985 0.168 0.1823 0.0919 0.092 0.1297 0.3275 0.1013 0.1919
None 0.2109 0.2728 0.2629 0.2773 0.2121 0.2726 0.1874 0.2389 0.2063 0.2461 0.1488 0.131 0.2461 0.2752 0.3111 0.0152 0.2764 0.1552
Nuclear Periphery 0.0058 0.0011 0.0013 0.0028 0.0018 0.0022 0.0191 0.0034 0.0041 0.002 0.0101 0.0049 0.015 0.0021 0.0023 0.0007 0.0072 0.0025
Nucleolus 0.0052 0.0018 0.0016 0.0044 0.0009 0.001 0.0044 0.0009 0.0018 0.0017 0.0012 0.0011 0.0007 0.0006 0.0014 0.0002 0.001 0.0006
Nucleus 0.0058 0.0018 0.0021 0.0025 0.0013 0.0026 0.0077 0.0018 0.0068 0.0024 0.0069 0.0034 0.0025 0.0013 0.0013 0.0005 0.0024 0.0019
Peroxisomes 0.0479 0.0379 0.038 0.0621 0.0529 0.0718 0.0303 0.0216 0.0426 0.0365 0.0398 0.0459 0.0619 0.0187 0.0571 0.0502 0.0411 0.0387
Punctate Nuclear 0.0212 0.0028 0.0021 0.0089 0.004 0.0047 0.0123 0.0025 0.0049 0.0017 0.0059 0.004 0.0033 0.0013 0.0029 0.0019 0.007 0.0014
Vacuole 0.0107 0.0073 0.006 0.0043 0.003 0.0076 0.0185 0.0098 0.022 0.0231 0.0194 0.0128 0.0091 0.0104 0.0072 0.0056 0.0242 0.0084
Vacuole Periphery 0.0036 0.0023 0.0028 0.0029 0.0009 0.0035 0.0096 0.0041 0.0063 0.0086 0.0089 0.0058 0.0046 0.0044 0.004 0.0051 0.0072 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5446 6.7445 12.5117 11.0569 7.3365 7.5405 11.7207 12.0818 9.7602 14.4901
Translational Efficiency 0.9404 0.7394 0.39 0.6608 0.6736 0.8644 0.8098 0.5694 0.8361 0.6617

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1191 306 1357 725 1574 915 1390 1021 2765 1221 2747 1746

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.90 762.44 792.29 806.24 674.22 762.98 777.48 839.60 679.25 762.84 784.80 825.75
Standard Deviation 79.94 100.43 88.57 107.35 77.18 90.93 93.54 111.35 78.59 93.41 91.42 110.93
Intensity Change Log 2 — 0.152626 0.208030 0.233211 — 0.178426 0.205586 0.316483 — 0.165473 0.206819 0.275090

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000406 0.001419 0.000459 0.008205 0.000512 0.000926 0.000358 0.012823 0.000466 0.001050 0.000408 0.010905
Bud Neck 0.034674 0.043621 0.015879 0.040250 0.033078 0.053837 0.023809 0.032361 0.033766 0.051276 0.019891 0.035637
Bud Site 0.008557 0.031806 0.007360 0.089348 0.011363 0.021709 0.011795 0.097030 0.010155 0.024239 0.009604 0.093840
Cell Periphery 0.000213 0.000246 0.000205 0.000261 0.000214 0.000379 0.000826 0.000332 0.000214 0.000345 0.000519 0.000302
Cytoplasm 0.120366* 0.101304* 0.302149* 0.160038* 0.143203* 0.115372* 0.309722* 0.203926* 0.133366* 0.111846* 0.305981* 0.185702*
Cytoplasmic Foci 0.356976* 0.328450* 0.109861 0.128147 0.386289* 0.287058* 0.136704 0.167690 0.373663* 0.297431* 0.123443 0.151270
Eisosomes 0.000298 0.000262 0.000116 0.000214 0.000327 0.000243 0.000798 0.000270 0.000315 0.000248 0.000461 0.000247
Endoplasmic Reticulum 0.002375 0.000861 0.003759 0.005467 0.002853 0.001128 0.001833 0.003620 0.002647 0.001061 0.002784 0.004387
Endosome 0.081827 0.092049 0.021276 0.032107 0.066865 0.135997 0.022106 0.031002 0.073309 0.124983 0.021696 0.031461
Golgi 0.041828 0.043163 0.001110 0.010815 0.042648 0.044091 0.002022 0.011943 0.042295 0.043858 0.001571 0.011475
Lipid Particles 0.042321 0.010930 0.012335 0.004387 0.037085 0.021413 0.016193 0.005371 0.039340 0.018786 0.014287 0.004962
Mitochondria 0.140104* 0.093591 0.004340 0.017782 0.119200* 0.090470 0.005783 0.018697 0.128204* 0.091252 0.005070 0.018317
Mitotic Spindle 0.005495 0.026652 0.010571 0.069510 0.005466 0.011227 0.008683 0.045496 0.005478 0.015093 0.009616 0.055467
None 0.001521 0.001147 0.007220 0.001815 0.001336 0.002009 0.011410 0.000903 0.001416 0.001793 0.009341 0.001281
Nuclear Periphery 0.001087 0.000452 0.003952 0.001200 0.000648 0.000847 0.001673 0.000700 0.000837 0.000748 0.002799 0.000908
Nuclear Periphery Foci 0.000475 0.000396 0.001899 0.002100 0.000382 0.000500 0.001343 0.001225 0.000422 0.000474 0.001618 0.001588
Nucleolus 0.002662 0.002556 0.007438 0.001792 0.001985 0.003985 0.006310 0.001610 0.002277 0.003627 0.006868 0.001685
Nucleus 0.035338 0.035752 0.398088* 0.118774 0.039950 0.042486 0.345733* 0.079123 0.037963 0.040798 0.371596* 0.095587
Peroxisomes 0.067955 0.111069* 0.011410 0.104712* 0.047549 0.065039 0.015615 0.115054* 0.056338 0.076574 0.013538 0.110759*
Vacuole 0.045575 0.065402 0.078507 0.197808 0.049585 0.089057 0.075286 0.167064 0.047858 0.083129 0.076877 0.179830
Vacuole Periphery 0.009949 0.008873 0.002065 0.005268 0.009462 0.012228 0.001999 0.003762 0.009671 0.011387 0.002032 0.004387

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.42 -1.38 -6.09 -4.84 -5.95 -2.18 1.79 -9.40 -8.90 -9.50 -3.63 0.11 -11.17 -10.19 -11.17
Bud Neck -1.42 6.96 0.31 1.59 -6.31 -4.94 3.44 1.04 5.74 -2.54 -5.06 7.24 0.99 5.72 -6.25
Bud Site -4.72 1.00 -11.91 -5.16 -12.09 -4.20 -0.16 -15.82 -12.06 -15.18 -6.37 0.64 -19.79 -13.16 -19.58
Cell Periphery -0.55 0.53 0.20 0.62 -0.20 -1.65 -7.08 -2.48 0.92 6.20 -1.74 -6.70 -2.16 1.19 5.70
Cytoplasm 1.45 -19.84 -5.56 -5.29 12.08 3.47 -19.52 -7.14 -9.48 10.16 3.20 -27.54 -9.09 -10.53 15.22
Cytoplasmic Foci 1.90 32.66 30.78 15.94 0.28 10.96 33.94 31.37 15.55 -1.67 10.11 47.45 43.34 22.59 -1.69
Eisosomes 0.79 9.82 4.16 1.36 -3.74 3.72 -1.68 3.37 -0.53 1.99 3.39 -0.95 5.22 1.01 1.66
Endoplasmic Reticulum 3.11 -3.55 -4.74 -9.16 -2.72 3.69 1.87 -2.27 -8.75 -6.37 4.60 -1.21 -4.91 -12.23 -5.57
Endosome -1.20 14.18 13.00 8.94 -1.03 -10.76 13.96 12.48 18.45 -1.37 -9.74 19.94 17.98 20.54 -1.77
Golgi -0.07 15.77 12.46 7.87 -4.78 -0.18 18.08 13.89 10.62 -6.39 -0.24 24.05 18.64 13.13 -8.10
Lipid Particles 9.59 11.44 15.18 3.27 8.82 5.97 9.33 15.51 9.47 9.23 9.83 14.75 21.67 10.12 12.51
Mitochondria 4.20 21.40 19.38 8.16 -3.71 4.09 22.90 20.03 13.05 -4.15 6.23 31.34 27.82 15.56 -5.64
Mitotic Spindle -3.56 -2.56 -8.49 -3.79 -7.47 -2.76 -1.98 -8.84 -6.91 -7.41 -4.48 -3.20 -12.22 -8.36 -10.50
None 0.60 -4.21 -0.42 -0.95 3.93 -1.09 -6.25 1.28 1.84 6.58 -0.81 -7.53 0.41 1.09 7.69
Nuclear Periphery 2.18 -8.11 -1.71 -4.65 7.89 -0.85 -6.36 -2.08 -0.69 3.53 0.39 -10.43 -2.61 -2.41 8.58
Nuclear Periphery Foci 0.57 -9.15 -3.18 -3.26 -0.45 -1.20 -4.75 -6.97 -4.88 0.18 -0.89 -9.10 -5.51 -4.89 -0.15
Nucleolus 0.27 -5.42 1.31 0.78 6.82 -3.14 -7.11 -0.04 2.27 5.12 -2.51 -8.67 0.60 2.66 8.06
Nucleus -0.08 -42.32 -13.34 -10.99 21.70 -1.03 -39.14 -10.17 -8.38 28.46 -1.20 -57.29 -16.60 -13.84 35.87
Peroxisomes -4.00 15.68 -0.68 3.22 -10.16 -3.77 11.34 -7.85 -4.39 -14.01 -4.50 19.24 -6.38 -1.61 -17.39
Vacuole -2.77 -9.27 -22.95 -17.33 -18.49 -7.89 -8.29 -22.59 -15.33 -17.23 -8.53 -12.38 -32.03 -22.52 -25.10
Vacuole Periphery 0.77 10.62 5.93 3.23 -1.48 -2.12 10.47 6.27 7.12 -0.97 -1.57 14.84 8.59 7.99 -1.66
Standard name
Human Ortholog
Description Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
Localization
Cell Percentages mitochondrion (62%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cbs2

Cbs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available