Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.08 0.05 0.1 0.06 0 0 0 0 0 0.06 0.05 – – – 0 0 0 0 0.05 0.05
Golgi 0 0 0.15 0.13 0.26 0 0 0 0 0 0 0 0.13 0.2 0.19 – – – 0 0 0 0.08 0.07 0.06
Mitochondria 0.94 0.93 0.76 0.88 0.65 0.72 0.76 0.71 0.89 0.86 0.86 0.86 0.76 0.82 0.73 – – – 0.83 0.85 0.81 0.8 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.05 0 0.07 0.1 0.1 0.11 0.13 0.13 0.11 0.12 0.12 0.1 0.05 – – – 0 0 0 0 0 0
Nucleolus 0.07 0 0.05 0 0.06 0.11 0.14 0.11 0.13 0.09 0.13 0.09 0.09 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0.08 0 0 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.18 0.12 0.09 0.17 0.16 0.06 0.09 0 0 0 0 0.06 0.11 0.22 – – – 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 4 1 4 0 3 0 2 1 0 1 0 2 1 – – – 1 0 0 0 1 4
Bud 1 1 2 5 2 3 3 3 5 9 6 12 0 1 0 – – – 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – – 1 0 0 0 1 1
Bud Site 0 0 0 1 0 1 0 1 5 1 4 3 0 0 0 – – – – – – – – –
Cell Periphery 1 5 7 3 3 3 1 5 2 2 0 4 2 4 6 – – – 0 0 0 0 0 0
Cytoplasm 1 3 1 4 5 7 8 14 3 7 6 2 6 5 5 – – – 0 0 0 0 1 1
Endoplasmic Reticulum 1 1 0 0 1 3 2 0 0 1 2 1 1 0 2 – – – 0 0 0 0 0 0
Endosome 7 4 11 5 16 19 38 20 1 7 10 15 4 8 9 – – – 7 7 7 3 9 10
Golgi 6 0 32 28 54 10 5 13 0 4 1 3 15 28 31 – – – 7 6 8 5 14 13
Mitochondria 211 169 160 186 136 250 291 241 362 336 321 371 85 117 121 – – – 197 157 203 62 135 159
Nucleus 0 0 2 1 2 5 2 6 2 1 4 4 1 1 0 – – – 0 0 1 0 1 0
Nuclear Periphery 14 4 11 9 14 34 37 37 53 52 42 50 13 14 8 – – – 2 0 2 1 2 5
Nucleolus 16 5 11 3 12 38 53 36 54 34 50 39 10 3 3 – – – 1 0 0 0 2 3
Peroxisomes 7 2 17 5 3 4 9 5 2 6 3 10 0 0 6 – – – 10 3 19 0 8 17
SpindlePole 1 1 7 2 7 6 11 4 14 10 6 15 3 6 0 – – – 0 1 2 0 1 2
Vac/Vac Membrane 30 33 26 18 36 55 23 31 16 17 8 14 7 15 37 – – – 2 7 2 2 11 5
Unique Cell Count 225 181 211 211 208 346 384 341 405 391 373 433 112 142 165 237 186 251 78 198 232
Labelled Cell Count 297 228 291 271 295 438 486 416 521 489 463 544 147 204 229 237 186 251 78 198 232


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 6.5 8.6 8.5 9.6 8.1 7.8 8.3 6.7 7.3 6.9 7.5 7.8 8.0 8.3 – – – 9.1 10.4 9.8
Std Deviation (1e-4) 1.6 1.1 1.5 1.4 1.9 1.6 1.6 1.5 1.5 1.7 1.6 1.7 1.9 1.5 1.6 – – – 2.0 2.7 1.9
Intensity Change (Log2) – – – -0.02 0.16 -0.09 -0.15 -0.06 -0.37 -0.24 -0.33 -0.2 -0.15 -0.1 -0.06 – – – 0.08 0.27 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery -1.3 0 0 0 0 0 0 0 0 0 0 0.2 – – –
Cytoplasm 0 0 0 0 2.6 0 0 0 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -1.5 1.0 0.1 2.0 0.3 0 -2.4 -1.6 -1.1 -0.7 0.2 0.1 – – –
Golgi -0.6 2.7 -5.3 -6.7 -4.7 -8.0 -7.0 -7.5 -7.6 -0.4 1.1 0.9 – – –
Mitochondria 3.3 -2.3 -0.9 0 -1.3 4.4 3.1 3.1 3.1 0 1.5 -0.6 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery -0.5 0.7 1.9 1.9 2.3 3.0 3.1 2.4 2.6 2.1 1.7 -0.2 – – –
Nucleolus -2.2 0.2 2.3 3.2 2.2 3.1 1.6 3.1 1.7 1.3 -1.5 -1.7 – – –
Peroxisomes -2.6 -3.2 -4.1 -3.3 -3.8 -5.2 -4.0 -4.6 -3.4 -3.1 -3.5 -1.8 – – –
SpindlePole 0 0 0 -0.3 0 0.1 -0.5 0 0.1 0 0.4 0 – – –
Vacuole -1.3 1.4 1.2 -2.7 -1.2 -3.9 -3.6 -5.0 -4.5 -1.7 -0.5 2.6 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5876 6.375 6.6714 6.375 6.8287 6.5182 9.2851 10.2189 9.8022 9.6986 8.7654 9.9833 7.4875 8.1554 7.5831 7.6206 7.3607 7.4055
Actin 0.0231 0.0024 0.0055 0.0018 0.0044 0.0005 0.0104 0.0035 0.012 0.002 0.0281 0.0005 0.0087 0.0015 0.0048 0.0265 0.0104 0.0097
Bud 0.0003 0.0001 0 0.0001 0.0006 0 0.0003 0.0002 0.0002 0 0.0004 0 0.0004 0.0001 0.0003 0.0002 0.0004 0.0001
Bud Neck 0.0002 0.0001 0 0 0.0004 0 0.0005 0.0001 0.0005 0 0.0002 0 0.0005 0 0.0001 0.0008 0.0002 0.0001
Bud Periphery 0.0005 0.0003 0.0001 0.0002 0.0012 0.0001 0.0007 0.0003 0.0005 0.0001 0.0006 0 0.001 0.0001 0.0008 0.0007 0.0006 0.0001
Bud Site 0.0007 0.0006 0.0001 0.0001 0.0004 0.0001 0.0024 0.0003 0.0052 0 0.0015 0 0.0009 0.0001 0.0004 0.0003 0.0035 0.0002
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0007 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.0032 0.0001 0 0 0.004 0 0.0074 0 0.0021 0 0.0004 0 0.0033 0 0.0039 0 0.0001 0.0001
Cytoplasmic Foci 0.0123 0.0023 0.002 0.0007 0.0299 0.0004 0.02 0.0029 0.0049 0.0003 0.0041 0.0001 0.0111 0.0014 0.0072 0.0013 0.0042 0.0033
Eisosomes 0.0007 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0008 0.0001 0.0002 0.0004 0.0002 0.0002 0.0004 0.0006 0.0002 0.0002
Endoplasmic Reticulum 0.0012 0.0001 0 0 0.0019 0 0.0021 0 0.0007 0 0.0002 0 0.0019 0 0.0017 0.0001 0.0002 0.0002
Endosome 0.0148 0.0148 0.0038 0.0016 0.0184 0.0012 0.0257 0.0037 0.0105 0.0012 0.0048 0.0018 0.0108 0.0066 0.01 0.0024 0.004 0.0056
Golgi 0.0145 0.0317 0.0178 0.0043 0.0612 0.0046 0.029 0.0086 0.0165 0.0028 0.0085 0.006 0.0103 0.0399 0.0117 0.0091 0.0153 0.0103
Lipid Particles 0.0524 0.0193 0.0037 0.0008 0.0225 0.0033 0.0348 0.0333 0.0063 0.0018 0.0063 0.0121 0.0217 0.0018 0.0088 0.0026 0.0081 0.0047
Mitochondria 0.8244 0.904 0.9555 0.985 0.7502 0.9826 0.8281 0.929 0.9188 0.9831 0.8908 0.9675 0.8896 0.9414 0.9323 0.9529 0.9392 0.9498
None 0.0082 0 0 0 0.0017 0 0.0033 0 0.0067 0 0.0001 0 0.0011 0 0.0007 0.0001 0.0001 0.0001
Nuclear Periphery 0.0093 0.0001 0 0 0.0185 0 0.0024 0 0.0004 0.0001 0.0001 0.0001 0.0017 0 0.001 0 0.0002 0.0002
Nucleolus 0.0096 0.0002 0 0 0.0014 0.0001 0.0011 0.0001 0.0003 0 0.0001 0.0001 0.0007 0 0.0001 0 0.0002 0.0001
Nucleus 0.0026 0.0001 0 0 0.0344 0 0.0007 0 0.0003 0 0.0001 0 0.0006 0 0.0001 0 0.0002 0.0001
Peroxisomes 0.0127 0.0111 0.0094 0.0041 0.0048 0.0031 0.0134 0.0154 0.0087 0.0029 0.052 0.0012 0.0231 0.004 0.0081 0.0013 0.0056 0.0072
Punctate Nuclear 0.0044 0 0 0 0.0158 0 0.0053 0 0.0005 0 0.0001 0 0.0019 0 0.0008 0 0.0005 0.0017
Vacuole 0.0014 0.0033 0.0003 0.0003 0.0163 0.0004 0.0036 0.0005 0.0014 0.0006 0.0004 0.0005 0.0066 0.0006 0.0014 0.0002 0.003 0.0017
Vacuole Periphery 0.0032 0.009 0.0013 0.0006 0.0117 0.0035 0.0081 0.0018 0.0018 0.0046 0.0011 0.0093 0.0035 0.0019 0.0051 0.0006 0.0037 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.4921 57.0083 39.3513 42.0375 47.1102 42.9852 53.9547 44.7846 63.756 49.2008
Translational Efficiency 0.8381 0.7576 0.9847 1.1964 0.8447 1.2013 1.0026 1.7749 1.1966 0.9793

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
601 587 341 1985 1786 2008 219 1910 2387 2595 560 3895

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 747.84 991.12 1418.52 1153.83 866.14 1037.73 1241.25 1280.46 836.35 1027.19 1349.19 1215.93
Standard Deviation 138.07 154.57 204.19 170.76 172.34 152.78 161.73 204.19 172.22 154.42 207.61 198.28
Intensity Change Log 2 — 0.406330 0.923585 0.625629 — 0.260759 0.519122 0.563990 — 0.330040 0.720679 0.592878

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001731 0.005832 0.008786 0.002250 0.001479 0.004795 0.011856 0.003497 0.001543 0.005030 0.009987 0.002862
Bud Neck 0.006769 0.001601 0.007047 0.001638 0.002474 0.000924 0.009818 0.000621 0.003555 0.001077 0.008131 0.001139
Bud Site 0.006713 0.004567 0.003388 0.008092 0.005108 0.002101 0.012847 0.002553 0.005513 0.002659 0.007087 0.005376
Cell Periphery 0.000952 0.000318 0.000169 0.000524 0.000579 0.000167 0.000255 0.000121 0.000673 0.000201 0.000202 0.000326
Cytoplasm 0.001862 0.000791 0.000698 0.001260 0.000772 0.000531 0.001573 0.000538 0.001046 0.000590 0.001040 0.000906
Cytoplasmic Foci 0.011657 0.027982 0.071251 0.011601 0.011647 0.017516 0.090422 0.008400 0.011650 0.019884 0.078748 0.010031
Eisosomes 0.000091 0.000149 0.000303 0.000074 0.000066 0.000084 0.000180 0.000165 0.000073 0.000099 0.000255 0.000119
Endoplasmic Reticulum 0.000470 0.000290 0.000660 0.000627 0.000481 0.000210 0.000688 0.000154 0.000479 0.000228 0.000671 0.000395
Endosome 0.036422 0.035589 0.023875 0.016320 0.019277 0.026751 0.050333 0.004926 0.023594 0.028750 0.034222 0.010733
Golgi 0.019002 0.043148 0.111040* 0.031880 0.014340 0.030334 0.063549 0.030294 0.015514 0.033232 0.092468 0.031102
Lipid Particles 0.008103 0.011035 0.013880 0.002580 0.010085 0.004545 0.012174 0.002287 0.009586 0.006013 0.013212 0.002436
Mitochondria 0.854620* 0.806374* 0.660389* 0.876967* 0.898144* 0.873985* 0.596932* 0.928790* 0.887186* 0.858691* 0.635573* 0.902380*
Mitotic Spindle 0.002436 0.002888 0.022847 0.006220 0.001645 0.002687 0.046602 0.002257 0.001844 0.002732 0.032137 0.004276
None 0.002305 0.000383 0.001460 0.000773 0.000559 0.000212 0.000376 0.000553 0.000999 0.000250 0.001036 0.000665
Nuclear Periphery 0.000099 0.000110 0.003110 0.001095 0.000440 0.000095 0.002035 0.000238 0.000354 0.000098 0.002690 0.000675
Nuclear Periphery Foci 0.000408 0.000234 0.002158 0.000498 0.000880 0.000147 0.002254 0.000160 0.000761 0.000167 0.002195 0.000332
Nucleolus 0.000362 0.000223 0.001097 0.000144 0.000510 0.000114 0.001023 0.000095 0.000472 0.000139 0.001068 0.000120
Nucleus 0.000331 0.000116 0.000347 0.000080 0.000274 0.000123 0.000566 0.000023 0.000288 0.000122 0.000433 0.000052
Peroxisomes 0.036960 0.047987 0.028440 0.004854 0.021245 0.027274 0.076352 0.006771 0.025201 0.031959 0.047177 0.005794
Vacuole 0.004395 0.006898 0.017861 0.013341 0.004975 0.003872 0.011192 0.003120 0.004829 0.004557 0.015253 0.008328
Vacuole Periphery 0.004310 0.003486 0.021194 0.019183 0.005018 0.003531 0.008974 0.004436 0.004840 0.003521 0.016416 0.011951

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.07 -6.33 -1.01 3.58 5.90 -3.84 -3.87 -3.21 1.35 3.08 -4.96 -6.74 -3.13 3.01 5.64
Bud Neck 2.73 -0.15 2.71 -0.10 5.47 2.76 -5.11 3.38 1.64 6.87 3.89 -4.64 3.77 -0.32 8.81
Bud Site 1.10 2.26 -0.83 -1.95 -3.51 2.76 -2.57 1.97 -0.56 3.46 3.03 -1.14 0.04 -3.27 1.22
Cell Periphery 2.10 2.67 1.37 -1.59 -3.28 3.08 2.39 3.42 2.24 4.73 3.76 3.77 2.55 -2.14 -2.18
Cytoplasm 1.11 1.21 0.56 -1.53 -1.99 0.88 -1.93 0.70 -0.08 2.48 1.43 0.00 0.33 -1.56 0.48
Cytoplasmic Foci -4.75 -9.99 0.04 5.32 10.34 -3.78 -8.94 2.47 6.42 9.34 -5.74 -13.55 1.44 7.55 13.97
Eisosomes -3.59 -7.47 1.39 5.88 8.62 -2.88 -4.72 -4.34 -3.54 0.51 -4.28 -9.61 -3.78 -1.60 6.29
Endoplasmic Reticulum 1.49 -1.42 -0.72 -1.69 0.16 2.21 -1.38 2.75 1.17 5.18 2.58 -1.76 0.62 -1.61 2.38
Endosome 0.16 2.67 4.93 5.00 2.56 -3.18 -5.58 8.59 12.04 8.48 -2.34 -3.43 7.57 10.43 8.44
Golgi -5.10 -9.54 -3.75 2.45 8.26 -7.16 -6.60 -5.76 0.01 4.28 -8.68 -11.80 -7.68 0.89 9.24
Lipid Particles -0.98 -2.18 2.82 3.73 6.06 3.93 -0.74 5.86 3.23 3.88 2.82 -1.98 6.43 4.79 7.16
Mitochondria 2.88 9.24 -1.81 -5.07 -11.48 3.13 11.12 -4.42 -7.86 -12.33 4.00 15.81 -2.46 -6.94 -17.14
Mitotic Spindle -0.37 -4.67 -3.15 -2.57 3.73 -1.99 -5.27 -1.20 0.61 5.19 -1.82 -7.08 -4.03 -2.34 6.46
None 1.31 0.59 1.05 -5.27 5.46 7.43 3.53 0.13 -12.15 -4.92 2.01 -0.08 0.90 -10.91 4.73
Nuclear Periphery -0.39 -5.98 -4.39 -4.36 3.66 2.17 -2.71 1.17 -2.09 3.15 2.15 -5.89 -1.90 -4.79 5.08
Nuclear Periphery Foci 1.12 -4.60 -0.55 -3.05 4.65 2.94 -1.72 2.82 -0.22 2.70 3.12 -3.46 2.21 -3.37 4.97
Nucleolus 1.16 -2.16 2.38 0.90 2.89 2.32 -0.83 2.44 0.61 1.48 2.55 -1.77 2.73 0.59 2.96
Nucleus 2.31 -0.13 2.98 0.78 2.48 2.10 -0.94 4.31 2.46 1.69 2.84 -1.02 4.84 2.06 2.66
Peroxisomes -1.55 1.27 7.06 7.74 4.68 -2.00 -5.13 6.47 8.45 6.56 -2.36 -3.97 9.76 11.79 7.89
Vacuole -1.41 -5.17 -6.71 -3.29 1.64 1.41 -3.00 2.13 0.89 3.86 0.38 -5.86 -4.37 -4.48 3.75
Vacuole Periphery 0.96 -6.30 -9.90 -10.91 0.69 2.52 -2.02 0.81 -1.50 2.30 2.68 -6.41 -8.50 -10.65 2.33
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rsm7

Rsm7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsm7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available