Standard name | |
---|---|
Human Ortholog | |
Description | DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 4 | 1 | 3 | 5 | 9 | 17 | 2 | 4 | 9 | 12 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 6 | 0 | 0 | 6 | 8 |
Bud Neck | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 7 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 2 | 8 | 10 | 20 | 20 | 9 | 14 | 15 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 15 | 19 | 12 | 33 | 34 | 69 | 73 | 46 | 40 | 36 | 47 | 17 | 34 | 48 | 54 | 40 | 41 | 3 | 3 | 4 | 0 | 1 | 8 |
Endoplasmic Reticulum | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 13 | 15 | 12 | 0 | 1 | 0 | 1 | 2 | 1 |
Endosome | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 17 | 13 | 6 | 5 | 7 | 1 | 1 | 6 | 8 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 4 |
Mitochondria | 3 | 29 | 190 | 137 | 148 | 192 | 379 | 370 | 219 | 279 | 264 | 311 | 5 | 3 | 16 | 18 | 4 | 22 | 4 | 7 | 7 | 0 | 1 | 6 |
Nucleus | 401 | 398 | 207 | 127 | 177 | 127 | 243 | 200 | 103 | 110 | 130 | 135 | 119 | 140 | 141 | 379 | 235 | 234 | 383 | 409 | 214 | 67 | 162 | 141 |
Nuclear Periphery | 1 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 4 | 0 | 0 | 1 | 1 | 1 | 4 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 3 | 1 | 0 | 23 | 12 | 8 | 6 | 20 | 15 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 3 | 7 | 2 | 11 | 4 | 19 | 11 | 7 | 7 | 7 | 13 | 1 | 6 | 2 | 9 | 9 | 6 | 8 | 7 | 6 | 1 | 6 | 13 |
Vac/Vac Membrane | 15 | 23 | 0 | 0 | 2 | 1 | 2 | 2 | 1 | 4 | 3 | 9 | 3 | 2 | 6 | 38 | 28 | 26 | 29 | 32 | 55 | 9 | 35 | 57 |
Unique Cell Count | 428 | 456 | 289 | 171 | 285 | 257 | 503 | 483 | 281 | 330 | 322 | 392 | 145 | 182 | 204 | 480 | 318 | 310 | 472 | 499 | 313 | 92 | 251 | 275 |
Labelled Cell Count | 440 | 483 | 431 | 283 | 385 | 375 | 744 | 704 | 389 | 462 | 467 | 545 | 148 | 190 | 219 | 537 | 347 | 348 | 472 | 499 | 313 | 92 | 251 | 275 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.2 | 3.3 | 3.3 | 3.6 | 3.3 | 3.0 | 3.0 | 2.7 | 2.6 | 2.7 | 2.7 | 5.4 | 5.4 | 4.7 | 7.4 | 7.5 | 7.3 | 5.3 | 5.7 | 6.1 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.4 | 0.7 | 1.3 | 1.4 | 1.0 | 1.0 | 1.3 | 1.1 | 1.1 | 1.2 | 1.3 | 1.5 | 1.4 | 2.2 | 1.7 | 1.6 | 1.8 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.12 | -0.02 | -0.13 | -0.15 | -0.27 | -0.33 | -0.29 | -0.28 | 0.71 | 0.71 | 0.51 | 1.16 | 1.18 | 1.14 | 0.68 | 0.79 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 2.1 | 2.6 | 3.1 | 3.5 | 3.7 | 2.3 | 2.0 | 2.4 | 1.8 | 4.0 | 5.4 | 2.1 | 2.5 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.5 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -12.3 | -13.8 | 0 | -18.7 | -17.0 | -15.0 |
Nucleus | 0.6 | -2.4 | -5.3 | -6.4 | -8.1 | -8.4 | -9.5 | -7.8 | -9.6 | 2.4 | 1.3 | -0.6 | 2.3 | 0.6 | 1.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | -0.5 | 0.3 | -0.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 5.2 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0187 | 1.8034 | 1.4883 | 1.2437 | 1.2946 | 1.4753 | 1.6282 | 2.7194 | 2.0068 | 2.1793 | 1.7845 | 2.1974 | 3.8364 | 4.2215 | 3.9643 | 4.1842 | 3.197 | 4.2882 |
Actin | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0226 | 0.0011 | 0.0032 | 0.0006 | 0.0046 | 0.0011 | 0.0089 | 0 | 0 | 0.0012 | 0.0004 | 0.0001 |
Bud | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0005 |
Bud Neck | 0.0004 | 0.0005 | 0.0003 | 0.0012 | 0.0014 | 0.0051 | 0.0012 | 0.0003 | 0.002 | 0.0066 | 0.0008 | 0.0014 | 0.0009 | 0.0002 | 0.0002 | 0.0114 | 0.008 | 0.0018 |
Bud Periphery | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0029 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0.0004 | 0 | 0 | 0.0011 | 0.0004 | 0.0001 |
Bud Site | 0.0002 | 0.0007 | 0.0001 | 0.0008 | 0.0005 | 0.0002 | 0.0026 | 0.0002 | 0.0014 | 0.005 | 0.0004 | 0.0004 | 0.003 | 0.0023 | 0.0002 | 0.0119 | 0.0006 | 0.0001 |
Cell Periphery | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0 |
Cytoplasm | 0.0023 | 0.008 | 0.002 | 0.0007 | 0.015 | 0.0036 | 0.0087 | 0.0126 | 0.0118 | 0.0085 | 0.0066 | 0.0055 | 0.0038 | 0.0034 | 0.0044 | 0.0019 | 0.0046 | 0.007 |
Cytoplasmic Foci | 0.0001 | 0.0003 | 0 | 0.0012 | 0.0037 | 0.0004 | 0.0109 | 0.0023 | 0.0085 | 0.0029 | 0.0032 | 0.0004 | 0.0011 | 0.0043 | 0.0001 | 0.0001 | 0.0003 | 0.0007 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0031 | 0.0002 | 0.0009 | 0.0004 | 0.0028 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0009 |
Endosome | 0.0001 | 0.0011 | 0.0001 | 0.0013 | 0.0067 | 0.0004 | 0.0174 | 0.0005 | 0.007 | 0.0087 | 0.0186 | 0.0009 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0013 |
Golgi | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0055 | 0.0002 | 0.0014 | 0.0027 | 0.0041 | 0.0004 | 0.0011 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Lipid Particles | 0 | 0.0001 | 0 | 0.0017 | 0.0018 | 0.0001 | 0.0311 | 0.0017 | 0.0079 | 0.0128 | 0.0047 | 0.0007 | 0.0016 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 |
Mitochondria | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0067 | 0.0003 | 0.0007 | 0.0048 | 0.0022 | 0.0021 | 0.0008 | 0.0002 | 0.0001 | 0.0007 | 0.0014 | 0.0001 |
None | 0.016 | 0.0111 | 0.0121 | 0.0004 | 0.0099 | 0.001 | 0.0698 | 0.0576 | 0.0887 | 0.1072 | 0.1319 | 0.0937 | 0.0031 | 0.0191 | 0.011 | 0.0011 | 0.0048 | 0.018 |
Nuclear Periphery | 0.0026 | 0.0089 | 0.0064 | 0.0023 | 0.0123 | 0.0017 | 0.0154 | 0.0034 | 0.0056 | 0.0047 | 0.0056 | 0.0036 | 0.003 | 0.0021 | 0.0023 | 0.0039 | 0.0047 | 0.0074 |
Nucleolus | 0.0082 | 0.0124 | 0.0108 | 0.0273 | 0.0288 | 0.029 | 0.0167 | 0.012 | 0.01 | 0.0329 | 0.0333 | 0.0221 | 0.0143 | 0.0168 | 0.0139 | 0.0208 | 0.0782 | 0.0269 |
Nucleus | 0.9609 | 0.946 | 0.9505 | 0.9042 | 0.8327 | 0.9352 | 0.7132 | 0.8743 | 0.8107 | 0.7724 | 0.6973 | 0.8148 | 0.9391 | 0.9154 | 0.9319 | 0.9282 | 0.8344 | 0.894 |
Peroxisomes | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0096 | 0.0051 | 0.0063 | 0.002 | 0.0012 | 0.0005 | 0.0009 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Punctate Nuclear | 0.0085 | 0.0079 | 0.0172 | 0.0568 | 0.0829 | 0.0224 | 0.0559 | 0.0276 | 0.0317 | 0.0201 | 0.0813 | 0.0509 | 0.0148 | 0.0351 | 0.0355 | 0.0157 | 0.0598 | 0.0403 |
Vacuole | 0.0002 | 0.0017 | 0.0001 | 0.0005 | 0.0019 | 0.0004 | 0.0035 | 0.0002 | 0.0015 | 0.005 | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0007 | 0.0004 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0004 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.5998 | 8.0316 | 9.6846 | 14.8192 | 15.289 | 15.9947 | 13.323 | 14.2162 | 14.4838 | 18.9272 |
Translational Efficiency | 1.243 | 1.3915 | 1.0691 | 0.6794 | 0.8144 | 1.1384 | 0.6999 | 0.5754 | 0.6425 | 0.7139 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1939 | 1459 | 1022 | 1100 | 1502 | 1854 | 405 | 3167 | 3441 | 3313 | 1427 | 4267 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 665.09 | 765.84 | 946.24 | 849.74 | 726.32 | 723.08 | 935.02 | 927.32 | 691.82 | 741.91 | 943.06 | 907.32 |
Standard Deviation | 79.97 | 111.76 | 121.50 | 133.52 | 81.76 | 106.41 | 104.31 | 136.07 | 86.28 | 110.85 | 116.98 | 139.60 |
Intensity Change Log 2 | — | 0.203493 | 0.508657 | 0.353472 | — | -0.006450 | 0.364392 | 0.352462 | — | 0.097719 | 0.435152 | 0.352945 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000041 | 0.000519 | 0.000599 | 0.001150 | 0.000114 | 0.000557 | 0.000495 | 0.000437 | 0.000073 | 0.000540 | 0.000570 | 0.000621 |
Bud Neck | 0.001496 | 0.007339 | 0.006513 | 0.019331 | 0.003017 | 0.011685 | 0.006152 | 0.042090 | 0.002160 | 0.009771 | 0.006411 | 0.036223 |
Bud Site | 0.000204 | 0.003305 | 0.004869 | 0.016221 | 0.000413 | 0.003666 | 0.001529 | 0.008449 | 0.000295 | 0.003507 | 0.003921 | 0.010452 |
Cell Periphery | 0.000104 | 0.000100 | 0.000113 | 0.000229 | 0.000071 | 0.000199 | 0.000054 | 0.000173 | 0.000090 | 0.000155 | 0.000096 | 0.000188 |
Cytoplasm | 0.039025 | 0.040478 | 0.074339 | 0.106127* | 0.018688 | 0.043721 | 0.007520 | 0.064519 | 0.030148 | 0.042293 | 0.055375 | 0.075245 |
Cytoplasmic Foci | 0.002104 | 0.004803 | 0.000645 | 0.008128 | 0.002541 | 0.006079 | 0.000211 | 0.000901 | 0.002295 | 0.005517 | 0.000522 | 0.002765 |
Eisosomes | 0.000012 | 0.000023 | 0.000022 | 0.000043 | 0.000016 | 0.000027 | 0.000032 | 0.000021 | 0.000013 | 0.000025 | 0.000025 | 0.000027 |
Endoplasmic Reticulum | 0.000923 | 0.003891 | 0.005573 | 0.006354 | 0.002200 | 0.003738 | 0.005324 | 0.002904 | 0.001481 | 0.003805 | 0.005502 | 0.003793 |
Endosome | 0.000165 | 0.001495 | 0.000529 | 0.015947 | 0.000330 | 0.001118 | 0.000380 | 0.000852 | 0.000237 | 0.001284 | 0.000487 | 0.004744 |
Golgi | 0.000036 | 0.000709 | 0.000065 | 0.003664 | 0.000133 | 0.000540 | 0.000056 | 0.000210 | 0.000078 | 0.000615 | 0.000062 | 0.001100 |
Lipid Particles | 0.000462 | 0.001198 | 0.000429 | 0.001545 | 0.001150 | 0.002335 | 0.000395 | 0.000207 | 0.000762 | 0.001834 | 0.000420 | 0.000552 |
Mitochondria | 0.000367 | 0.004148 | 0.001058 | 0.008737 | 0.003017 | 0.006724 | 0.000715 | 0.006671 | 0.001524 | 0.005589 | 0.000961 | 0.007204 |
Mitotic Spindle | 0.000028 | 0.006004 | 0.002618 | 0.083958 | 0.001462 | 0.007511 | 0.001139 | 0.021336 | 0.000654 | 0.006847 | 0.002198 | 0.037480 |
None | 0.013464 | 0.006739 | 0.006493 | 0.020285 | 0.006575 | 0.011633 | 0.004804 | 0.008644 | 0.010457 | 0.009478 | 0.006014 | 0.011645 |
Nuclear Periphery | 0.000468 | 0.002018 | 0.001153 | 0.004600 | 0.001492 | 0.002334 | 0.000667 | 0.002457 | 0.000915 | 0.002195 | 0.001015 | 0.003009 |
Nuclear Periphery Foci | 0.000163 | 0.000667 | 0.000843 | 0.004142 | 0.000429 | 0.000827 | 0.000199 | 0.000404 | 0.000279 | 0.000757 | 0.000661 | 0.001368 |
Nucleolus | 0.006404 | 0.007748 | 0.000875 | 0.008855 | 0.010955 | 0.011789 | 0.003366 | 0.006493 | 0.008390 | 0.010009 | 0.001582 | 0.007102 |
Nucleus | 0.933745* | 0.903653* | 0.881763* | 0.641234* | 0.945603* | 0.878571* | 0.962034* | 0.822345* | 0.938921* | 0.889617* | 0.904545* | 0.775656* |
Peroxisomes | 0.000129 | 0.001293 | 0.000128 | 0.001031 | 0.000691 | 0.002762 | 0.000187 | 0.000438 | 0.000374 | 0.002115 | 0.000145 | 0.000591 |
Vacuole | 0.000599 | 0.003287 | 0.011264 | 0.035971 | 0.000848 | 0.003282 | 0.004617 | 0.009131 | 0.000708 | 0.003284 | 0.009378 | 0.016050 |
Vacuole Periphery | 0.000062 | 0.000584 | 0.000107 | 0.012447 | 0.000254 | 0.000903 | 0.000122 | 0.001318 | 0.000146 | 0.000762 | 0.000111 | 0.004187 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.63 | -15.44 | -14.68 | -5.23 | -6.10 | -2.73 | -9.91 | -6.02 | 0.53 | 3.80 | -4.32 | -17.95 | -12.58 | -1.28 | 1.02 |
Bud Neck | -7.81 | -5.55 | -15.00 | -10.04 | -9.35 | -7.10 | -4.42 | -26.89 | -17.65 | -16.16 | -10.19 | -6.75 | -30.67 | -21.38 | -21.38 |
Bud Site | -4.13 | -4.19 | -8.90 | -5.71 | -4.59 | -4.93 | -3.55 | -10.38 | -2.88 | -3.40 | -6.48 | -4.96 | -13.79 | -5.69 | -3.93 |
Cell Periphery | 0.17 | -1.03 | -4.35 | -5.20 | -3.61 | -2.60 | 0.22 | -4.21 | 1.76 | -4.67 | -2.33 | -1.52 | -4.07 | 1.03 | -2.33 |
Cytoplasm | -0.45 | -6.05 | -6.99 | -6.45 | -1.65 | -8.68 | 5.09 | -10.82 | -0.63 | -16.16 | -5.05 | -6.13 | -9.44 | -4.11 | -1.22 |
Cytoplasmic Foci | -2.91 | 3.83 | -0.23 | 2.71 | -4.00 | -3.82 | 4.62 | 3.70 | 6.88 | -2.05 | -4.85 | 5.67 | 3.65 | 7.79 | -2.96 |
Eisosomes | -6.07 | -9.07 | -21.09 | -13.58 | -11.86 | -6.00 | -7.00 | -5.66 | 2.48 | 5.40 | -9.02 | -10.99 | -19.21 | -5.46 | 0.07 |
Endoplasmic Reticulum | -10.07 | -15.71 | -22.30 | -12.33 | -5.06 | -3.62 | -7.89 | -3.46 | 1.19 | 6.93 | -9.29 | -16.01 | -17.57 | -5.32 | 6.20 |
Endosome | -3.07 | -9.39 | -5.06 | -3.33 | -4.45 | -5.04 | -3.81 | -5.95 | 2.14 | -0.23 | -4.97 | -9.20 | -6.41 | -1.77 | -4.35 |
Golgi | -2.99 | -3.32 | -4.30 | -1.05 | -4.14 | -2.36 | 0.11 | -1.29 | 2.00 | -2.06 | -3.86 | -0.52 | -4.99 | 1.36 | -5.03 |
Lipid Particles | -4.14 | -0.66 | -4.26 | 0.41 | -4.07 | -2.71 | 4.38 | 6.51 | 4.88 | 3.58 | -4.12 | 3.37 | 4.01 | 5.41 | 1.10 |
Mitochondria | -4.26 | -3.56 | -5.46 | -0.25 | -4.43 | -3.16 | 2.57 | -2.30 | 1.67 | -8.90 | -5.68 | 1.37 | -6.63 | 0.90 | -9.65 |
Mitotic Spindle | -4.10 | -2.82 | -11.98 | -10.49 | -11.06 | -3.77 | -1.46 | -12.51 | -8.41 | -10.82 | -5.72 | -2.85 | -17.71 | -13.36 | -15.22 |
None | 4.94 | 6.56 | 1.24 | -3.02 | -4.34 | -3.34 | 2.03 | -2.21 | 2.25 | -6.07 | 1.03 | 6.44 | 1.21 | -0.00 | -7.80 |
Nuclear Periphery | -8.70 | -10.47 | -12.14 | -7.56 | -8.80 | -2.78 | 0.68 | -9.16 | -5.09 | -11.60 | -7.35 | -5.55 | -17.90 | -8.95 | -14.11 |
Nuclear Periphery Foci | -2.87 | -6.36 | -5.18 | -3.48 | -2.51 | -2.09 | -1.38 | -1.16 | 1.22 | 1.13 | -3.84 | -3.99 | -5.25 | -1.52 | 0.85 |
Nucleolus | -0.48 | 7.65 | -4.03 | -3.77 | -7.34 | -0.35 | 0.97 | -4.73 | -4.83 | -4.07 | -1.05 | 6.02 | -7.40 | -6.74 | -11.87 |
Nucleus | 4.99 | 8.89 | 23.76 | 19.37 | 16.85 | 11.16 | 1.29 | 23.34 | 9.10 | 15.51 | 11.42 | 9.15 | 31.88 | 18.74 | 18.20 |
Peroxisomes | -4.01 | 0.58 | -3.36 | 3.00 | -3.56 | -3.90 | 2.61 | 2.73 | 4.85 | 0.27 | -5.44 | 3.05 | 1.05 | 5.74 | -1.90 |
Vacuole | -4.64 | -10.77 | -15.16 | -14.31 | -8.70 | -5.89 | -4.99 | -12.07 | -7.93 | 0.66 | -7.35 | -11.82 | -18.30 | -15.64 | -1.92 |
Vacuole Periphery | -3.80 | -3.80 | -3.07 | -2.53 | -3.01 | -2.33 | 1.08 | -1.75 | 0.69 | -2.23 | -3.74 | 0.29 | -3.76 | -1.58 | -3.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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