Standard name
Human Ortholog
Description Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.09 0 0 0 0 0 – 0 0 0 0 0 0.08 0.11 0.12 0 0.06 0.06 0.05 0.11 0.1
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.28 0.19 0.27 0 0.06 0.1 0 – 0 0.09 0 0.05 0 0.62 0.53 0.62 0.06 0.18 0.14 0.11 0.09 0.07
Mitochondria 0.94 0.88 0.78 0.89 0.79 0.86 0.82 0.67 0.89 – 0.88 0.87 0.97 0.94 0.92 0.05 0.05 0.07 0.85 0.63 0.73 0.66 0.65 0.64
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0.17 0.09 0.08 0.12 0.19 0.21 0.25 0.18 – 0.13 0.1 0.13 0.06 0.06 0.17 0.21 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.08 0.08 0.07 0.09 0.06 0 0.08 0 – 0 0 0.06 0.08 0.05 0.16 0.11 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 25 34 16 7 14 12 3 – 18 12 5 8 10 5 2 7 5 13 25 5 39 41
Bud 1 0 2 5 7 1 8 4 5 – 10 9 1 2 0 0 0 1 1 0 0 0 3 7
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 1 0 0 2 – 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 4 5 6 4 1 3 2 2 – 9 9 3 2 3 1 1 5 0 0 1 0 0 1
Cytoplasm 0 0 1 5 6 5 9 5 3 – 9 8 1 3 9 0 0 2 0 2 0 0 0 0
Endoplasmic Reticulum 1 1 1 1 1 1 1 1 2 – 8 5 2 1 5 0 0 1 0 1 1 0 2 1
Endosome 0 0 0 0 0 1 0 0 0 – 2 3 0 0 0 1 0 0 0 2 2 2 0 2
Golgi 3 8 103 72 153 20 47 65 10 – 24 67 2 22 19 40 10 35 14 42 58 11 30 30
Mitochondria 222 182 285 343 442 499 662 417 504 – 724 629 345 446 488 3 1 4 206 147 300 63 228 263
Nucleus 0 1 1 0 1 3 3 3 1 – 2 1 1 2 1 0 1 1 0 0 0 0 0 0
Nuclear Periphery 2 1 2 1 2 12 21 20 17 – 43 34 0 0 1 0 0 1 1 0 2 0 1 4
Nucleolus 53 36 32 32 70 111 171 155 100 – 106 75 46 27 32 11 4 7 4 4 4 4 7 8
Peroxisomes 0 3 3 1 4 1 4 3 0 – 4 5 0 0 3 0 0 0 3 6 7 1 30 36
SpindlePole 1 0 0 0 0 0 1 1 1 – 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 14 16 31 28 51 34 36 53 22 – 19 20 20 36 26 10 2 8 2 6 5 1 2 8
Unique Cell Count 235 207 367 385 561 581 804 627 567 827 724 356 475 528 64 19 56 242 233 410 97 353 413
Labelled Cell Count 302 255 491 528 757 697 980 741 672 978 882 426 549 597 71 21 72 242 233 410 97 353 413


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 46.9 56.0 46.0 48.9 44.0 33.5 31.0 28.8 24.8 – 20.9 22.3 59.2 57.2 49.5 59.2 56.4 59.7 42.0 46.7 46.3
Std Deviation (1e-4) 12.3 17.0 12.0 13.9 11.5 10.0 9.5 8.0 7.6 – 6.5 6.5 14.3 14.2 13.6 19.9 15.5 20.9 15.0 16.9 17.8
Intensity Change (Log2) – – – 0.09 -0.07 -0.46 -0.57 -0.68 -0.89 – -1.14 -1.05 0.36 0.31 0.1 0.36 0.29 0.37 -0.13 0.02 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -2.9 -4.7 -4.5 -3.9 -5.5 – -4.0 -4.4 -3.6 -3.8 -3.7 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.2 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi -3.0 -0.3 -11.0 -10.6 -7.2 -12.0 – -13.0 -8.1 -10.5 -9.5 -10.5 5.4 2.3 5.1
Mitochondria 4.2 0.4 3.3 1.9 -3.7 4.6 – 4.4 3.9 7.7 6.9 6.3 -11.4 -7.0 -10.6
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.9 2.4 2.7 2.6 – 3.9 3.6 0 0 0 0 0 0
Nucleolus -0.2 1.8 4.4 5.3 6.2 3.8 – 2.0 0.9 1.8 -1.7 -1.5 2.1 0 0.9
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -0.6 0.3 -1.5 -2.7 0 -2.9 – -4.9 -4.2 -1.5 -0.5 -2.1 1.8 0 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 84.1282 95.5795 84.3185 90.4368 78.2061 87.5043 96.5007 106.6251 101.804 68.031 91.2089 108.577 86.0092 103.8651 93.9794 90.1416 95.659 91.0307
Actin 0.0025 0.0004 0.0113 0.0007 0.0022 0.0003 0.0084 0.0011 0.0008 0.0004 0.0143 0.0002 0.016 0.0016 0.0095 0.0148 0.0011 0.008
Bud 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0 0.0002 0.0015 0 0.0001 0.0004 0.0003 0.0002 0 0.0001
Bud Neck 0.0005 0 0.0015 0 0.008 0 0.0001 0 0 0.0001 0.0005 0 0.0002 0 0.0005 0.0017 0 0
Bud Periphery 0.0001 0 0.0002 0 0.0007 0 0.0001 0 0 0.0002 0.0014 0 0.0001 0.0003 0.0003 0.0004 0 0.0002
Bud Site 0.0003 0 0.0059 0 0.0006 0 0.0002 0.0001 0.0001 0.0008 0.0234 0 0.0004 0.0007 0.0043 0.022 0 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0 0.0011 0.0001 0.0003 0.0002 0.0003 0.0004 0.0001 0 0.0006 0.0003 0.0003 0.0008 0.0001 0.0001
Cytoplasm 0.0012 0 0.0006 0 0.001 0 0.0003 0 0.0001 0.0012 0.0004 0 0.0021 0 0.0005 0.0028 0 0.0001
Cytoplasmic Foci 0.0047 0 0.0028 0 0.0181 0 0.0065 0.0001 0.006 0.022 0.0197 0 0.0185 0.0032 0.0087 0.005 0.002 0.0015
Eisosomes 0.0028 0.0013 0.0066 0.0023 0.0019 0.0005 0.0021 0.0021 0.0029 0.0035 0.0005 0.0006 0.0019 0.0013 0.0041 0.001 0.0005 0.0005
Endoplasmic Reticulum 0.0011 0 0.0003 0 0.0002 0.0001 0.0003 0 0 0.0004 0.0003 0 0.002 0 0.001 0.0023 0 0.0004
Endosome 0.0066 0.0001 0.0041 0 0.0157 0.0004 0.0016 0.0001 0.0002 0.0238 0.0042 0 0.0064 0.0001 0.0044 0.0041 0 0.0038
Golgi 0.0023 0.0003 0.0016 0.0001 0.0044 0.0002 0.003 0.0006 0.0005 0.0038 0.0045 0.0001 0.0034 0.0007 0.0019 0.0035 0 0.0043
Lipid Particles 0.0082 0.0055 0.0076 0.001 0.0048 0.0059 0.007 0.0023 0.0051 0.0071 0.016 0.0058 0.011 0.0039 0.01 0.0274 0.0013 0.0049
Mitochondria 0.9242 0.9833 0.935 0.9942 0.9277 0.95 0.9415 0.987 0.9529 0.8929 0.8742 0.9814 0.8863 0.9707 0.93 0.8919 0.9781 0.9694
None 0.0065 0 0.0029 0 0.0003 0 0.0011 0 0.0017 0.0059 0.0005 0 0.0033 0.0001 0.0006 0.0009 0.0001 0.0004
Nuclear Periphery 0.0033 0 0.0014 0 0.0002 0.0121 0.0005 0 0.0002 0.0007 0.0009 0 0.0091 0 0.0019 0.0023 0 0.0014
Nucleolus 0.0135 0.0002 0.0016 0 0.0007 0.0002 0.0166 0.0004 0.0089 0.0229 0.0081 0.0001 0.0197 0.0022 0.0015 0.0003 0.0124 0.001
Nucleus 0.004 0 0.0007 0 0.0004 0.0009 0.0011 0 0.0004 0.0015 0.0003 0 0.0018 0.0001 0.0006 0.0008 0.0003 0.0002
Peroxisomes 0.0034 0.0013 0.006 0.0006 0.0051 0.0003 0.0034 0.0014 0.0081 0.0018 0.0186 0.0004 0.0075 0.0029 0.0049 0.0029 0.0023 0.0016
Punctate Nuclear 0.0037 0 0.0033 0 0.0024 0.0002 0.0018 0 0.0002 0.001 0.0087 0 0.0036 0 0.0018 0.0029 0 0.0005
Vacuole 0.001 0.0004 0.0005 0 0.002 0.0003 0.0005 0.0006 0.0015 0.005 0.0004 0.0001 0.0015 0.0011 0.0011 0.0008 0.0002 0.0002
Vacuole Periphery 0.0096 0.0068 0.0058 0.0009 0.0024 0.0283 0.0035 0.004 0.0098 0.0045 0.0015 0.0112 0.0044 0.0103 0.0117 0.0112 0.0013 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 177.2761 179.5565 144.8399 124.771 147.0466 149.3697 162.628 134.9809 131.6359 124.369
Translational Efficiency 1.0441 1.0834 0.9994 1.1468 0.9672 1.3005 1.0461 1.1795 0.9165 1.0022

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1161 683 1845 1218 213 294 311 430 1374 977 2156 1648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2634.05 3691.46 5080.53 2881.91 3328.45 3354.28 3878.52 2658.44 2741.70 3590.00 4907.14 2823.60
Standard Deviation 798.70 1261.48 1218.06 740.93 931.33 1213.96 781.78 596.83 858.28 1256.92 1239.42 712.96
Intensity Change Log 2 — 0.486909 0.947696 0.129743 — 0.011153 0.220656 -0.324271 — 0.240834 0.587428 -0.105940

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009242 0.009128 0.003689 0.008099 0.012614 0.016829 0.011547 0.010739 0.009765 0.011445 0.004823 0.008788
Bud Neck 0.001150 0.002230 0.001199 0.001965 0.004544 0.003872 0.005462 0.004129 0.001677 0.002724 0.001814 0.002530
Bud Site 0.000838 0.001189 0.000588 0.003172 0.001567 0.003853 0.002502 0.008181 0.000951 0.001991 0.000864 0.004479
Cell Periphery 0.000116 0.000115 0.000074 0.000338 0.000133 0.000415 0.000124 0.002005 0.000118 0.000205 0.000081 0.000773
Cytoplasm 0.000288 0.000204 0.000132 0.000354 0.000267 0.000531 0.000291 0.000460 0.000285 0.000302 0.000155 0.000382
Cytoplasmic Foci 0.030384 0.030039 0.019831 0.010821 0.053273 0.045743 0.037842 0.015122 0.033933 0.034765 0.022429 0.011944
Eisosomes 0.010710 0.007877 0.003187 0.001651 0.014348 0.010821 0.002814 0.002224 0.011274 0.008763 0.003133 0.001801
Endoplasmic Reticulum 0.000141 0.000120 0.000026 0.000142 0.000258 0.000170 0.000092 0.000600 0.000159 0.000135 0.000036 0.000261
Endosome 0.000211 0.000380 0.000118 0.001305 0.000844 0.000856 0.001004 0.001789 0.000309 0.000523 0.000246 0.001431
Golgi 0.009083 0.010807 0.005560 0.016921 0.006241 0.017480 0.016642 0.027139 0.008643 0.012815 0.007158 0.019587
Lipid Particles 0.008706 0.004940 0.006674 0.003800 0.005180 0.007576 0.009646 0.017058 0.008159 0.005733 0.007102 0.007260
Mitochondria 0.879432* 0.892220* 0.929835* 0.916110* 0.838736* 0.823782* 0.857624* 0.845124* 0.873123* 0.871625* 0.919418* 0.897588*
Mitotic Spindle 0.000531 0.000642 0.001720 0.001978 0.000617 0.002477 0.005030 0.002354 0.000544 0.001194 0.002197 0.002076
None 0.009485 0.004840 0.006097 0.004388 0.006062 0.006303 0.004762 0.005550 0.008954 0.005280 0.005905 0.004691
Nuclear Periphery 0.000239 0.000262 0.001017 0.000614 0.000540 0.000524 0.001520 0.002832 0.000286 0.000341 0.001089 0.001193
Nuclear Periphery Foci 0.000162 0.000158 0.000166 0.000188 0.000431 0.000407 0.000360 0.000768 0.000204 0.000233 0.000194 0.000339
Nucleolus 0.002125 0.002899 0.001130 0.000846 0.002563 0.003435 0.002597 0.001195 0.002193 0.003060 0.001342 0.000937
Nucleus 0.000197 0.000168 0.000044 0.000163 0.000147 0.000581 0.000169 0.000216 0.000189 0.000292 0.000062 0.000177
Peroxisomes 0.024662 0.021532 0.009918 0.005090 0.039557 0.030488 0.020603 0.003978 0.026971 0.024227 0.011459 0.004800
Vacuole 0.002858 0.005277 0.002324 0.006978 0.005865 0.010280 0.007129 0.012848 0.003324 0.006782 0.003017 0.008510
Vacuole Periphery 0.009439 0.004973 0.006672 0.015075 0.006212 0.013578 0.012240 0.035688 0.008938 0.007563 0.007475 0.020454

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.06 3.82 0.58 0.50 -3.24 -0.71 0.22 0.39 1.20 0.21 -0.78 3.44 0.53 1.27 -3.11
Bud Neck -2.82 -0.23 -1.74 0.39 -1.64 0.48 -0.60 0.26 -0.25 0.88 -2.63 -0.48 -1.97 0.30 -1.61
Bud Site -1.08 0.96 -2.93 -2.49 -3.30 -1.51 -1.10 -2.47 -1.47 -2.13 -2.10 0.33 -3.88 -2.52 -4.02
Cell Periphery 0.04 0.91 -3.35 -3.20 -3.40 -1.07 0.16 -1.82 -1.50 -1.83 -1.06 0.93 -2.41 -2.01 -2.53
Cytoplasm 1.79 3.47 -0.91 -2.16 -3.19 -2.04 -0.44 -0.99 0.26 -0.87 -0.33 3.32 -1.28 -1.05 -3.12
Cytoplasmic Foci 0.09 3.76 7.19 5.79 4.53 0.74 1.61 4.53 4.71 4.07 -0.23 4.11 8.30 7.52 5.59
Eisosomes 2.54 10.06 12.29 7.30 6.81 0.94 3.48 3.65 4.57 1.12 2.21 9.99 11.69 8.48 6.13
Endoplasmic Reticulum 0.74 6.34 -0.06 -0.71 -5.00 0.55 1.03 -1.40 -2.30 -2.73 0.71 4.22 -1.75 -2.34 -4.39
Endosome -2.45 2.26 -3.41 -2.87 -3.72 -0.04 -0.31 -1.58 -2.23 -1.68 -2.04 0.67 -4.21 -3.44 -4.59
Golgi -0.86 2.78 -3.56 -2.49 -5.96 -3.48 -3.69 -4.86 -1.91 -2.18 -2.37 1.28 -5.42 -3.00 -6.83
Lipid Particles 2.30 1.30 3.29 1.09 3.10 -1.17 -1.35 -2.73 -2.11 -1.42 1.70 0.75 0.53 -1.08 -0.12
Mitochondria -1.30 -6.94 -4.40 -2.57 2.27 0.66 -0.84 -0.27 -1.06 0.70 0.17 -6.58 -3.03 -2.93 3.58
Mitotic Spindle -0.53 -3.42 -1.74 -1.62 -0.32 -1.02 -4.37 -2.62 0.08 2.25 -1.14 -4.99 -2.38 -1.04 0.14
None 7.26 5.64 8.70 1.11 4.99 -0.24 1.46 0.58 1.01 -1.27 6.54 5.78 8.27 1.63 3.96
Nuclear Periphery -0.42 -4.46 -4.30 -4.13 2.15 0.08 -2.73 -2.99 -3.04 -1.59 -0.96 -5.08 -4.33 -4.07 -0.40
Nuclear Periphery Foci 0.11 -0.09 -0.58 -0.85 -0.65 0.15 0.46 -1.65 -1.93 -2.18 -0.64 0.24 -2.27 -1.86 -2.76
Nucleolus -0.93 2.75 3.49 2.71 1.63 -0.94 -0.06 2.18 2.84 1.92 -1.34 2.55 3.86 3.67 2.20
Nucleus 0.59 4.09 0.33 -0.03 -1.72 -1.81 -0.42 -1.17 1.48 -0.64 -1.26 3.89 0.11 1.17 -2.13
Peroxisomes 0.78 5.06 6.78 5.32 3.42 0.94 2.08 4.47 4.67 3.57 0.74 5.48 8.16 7.04 4.96
Vacuole -1.88 1.03 -4.15 -1.16 -4.82 -1.65 -0.63 -3.06 -0.83 -2.16 -2.92 0.55 -5.57 -1.26 -5.92
Vacuole Periphery 2.71 1.72 -2.68 -6.04 -5.13 -1.92 -1.80 -5.97 -3.84 -4.29 0.80 1.01 -5.52 -6.38 -7.22
Standard name
Human Ortholog
Description Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Lys4

Lys4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available