Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 10 | 3 | 0 | 2 | 4 | 0 | 0 | 0 | 2 | 2 | 5 | 27 | 7 | 1 | 10 | 6 | 15 | 7 | 14 | 8 | 6 | 8 | 8 |
Bud | 9 | 5 | 22 | 13 | 24 | 28 | 26 | 37 | 35 | 35 | 42 | 59 | 4 | 7 | 3 | 14 | 10 | 10 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 2 | 7 | 4 | 5 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 3 | 3 | 2 | 3 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 27 | 49 | 48 | 38 | 35 | 52 | 34 | 24 | 28 | 23 | 22 | 7 | 24 | 34 | 27 | 16 | 22 | 0 | 5 | 7 | 1 | 4 | 4 |
Endoplasmic Reticulum | 15 | 22 | 8 | 3 | 3 | 9 | 5 | 1 | 4 | 2 | 3 | 6 | 13 | 4 | 8 | 12 | 6 | 16 | 1 | 0 | 4 | 2 | 2 | 1 |
Endosome | 58 | 40 | 87 | 56 | 81 | 9 | 17 | 24 | 3 | 13 | 3 | 2 | 76 | 175 | 179 | 146 | 196 | 163 | 92 | 112 | 148 | 108 | 64 | 62 |
Golgi | 56 | 65 | 4 | 3 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 155 | 87 | 46 | 74 | 65 | 84 | 247 | 222 | 176 | 197 | 121 | 186 |
Mitochondria | 304 | 266 | 307 | 212 | 353 | 430 | 488 | 529 | 464 | 489 | 480 | 546 | 101 | 134 | 81 | 70 | 37 | 38 | 40 | 90 | 81 | 56 | 31 | 32 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 3 | 8 | 4 | 1 | 5 | 0 | 2 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 1 | 3 | 6 | 1 | 1 | 5 | 5 | 6 | 0 | 0 | 2 | 1 | 4 | 3 | 0 | 1 | 1 | 1 | 1 | 0 |
Nucleolus | 4 | 1 | 0 | 1 | 2 | 7 | 12 | 10 | 13 | 13 | 6 | 10 | 0 | 2 | 3 | 10 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 10 | 16 | 48 | 42 | 56 | 16 | 32 | 53 | 20 | 25 | 24 | 40 | 16 | 80 | 89 | 26 | 56 | 40 | 39 | 31 | 57 | 33 | 27 | 25 |
SpindlePole | 9 | 9 | 32 | 32 | 43 | 18 | 28 | 34 | 29 | 56 | 24 | 33 | 17 | 40 | 43 | 103 | 82 | 78 | 2 | 2 | 5 | 0 | 1 | 1 |
Vac/Vac Membrane | 21 | 17 | 10 | 5 | 21 | 10 | 16 | 15 | 10 | 6 | 15 | 5 | 10 | 25 | 30 | 24 | 21 | 22 | 7 | 10 | 19 | 14 | 6 | 10 |
Unique Cell Count | 376 | 384 | 443 | 320 | 477 | 487 | 583 | 622 | 514 | 559 | 524 | 601 | 310 | 418 | 385 | 376 | 364 | 383 | 445 | 498 | 517 | 429 | 270 | 340 |
Labelled Cell Count | 505 | 481 | 573 | 416 | 628 | 580 | 697 | 745 | 612 | 684 | 633 | 755 | 429 | 588 | 523 | 522 | 507 | 500 | 445 | 498 | 517 | 429 | 270 | 340 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.0 | 4.7 | 5.0 | 4.8 | 4.6 | 4.4 | 4.5 | 4.3 | 4.1 | 4.2 | 4.4 | 7.7 | 7.3 | 7.5 | 7.3 | 7.0 | 7.2 | 7.8 | 8.1 | 8.2 |
Std Deviation (1e-4) | 0.8 | 1.2 | 0.9 | 1.4 | 1.1 | 1.3 | 1.6 | 1.3 | 1.0 | 1.0 | 1.1 | 1.1 | 1.0 | 1.1 | 1.3 | 1.2 | 1.2 | 1.1 | 1.2 | 2.1 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.03 | -0.03 | -0.08 | -0.05 | -0.12 | -0.19 | -0.14 | -0.08 | 0.72 | 0.65 | 0.69 | 0.65 | 0.59 | 0.63 | 0.74 | 0.8 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.5 | 0 | 0 | 2.3 | 0 | 3.2 |
Bud | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -2.7 | -3.5 | -0.9 | -1.6 | -1.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | -1.6 | -2.1 | -1.1 | -3.4 | -3.7 | -3.6 | -3.9 | -4.7 | -4.5 | -2.8 | -1.1 | -1.9 | -3.5 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | -1.1 | 0.3 | 1.3 | -0.2 | 2.0 |
Endosome | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 7.1 | 8.3 | 6.1 | 10.1 | 7.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16.2 | 9.5 | 6.7 | 9.1 | 8.6 | 9.8 |
Mitochondria | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.0 | -10.9 | -13.9 | -14.5 | -16.9 | -17.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | 3.4 | 4.7 | -2.0 | 1.9 | -0.2 |
SpindlePole | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | 1.2 | 2.0 | 7.8 | 6.2 | 5.5 |
Vacuole | -0.7 | 1.8 | -0.2 | 0.5 | 0.2 | -0.3 | -1.5 | 0.6 | -1.9 | 0.8 | 2.8 | 3.7 | 2.9 | 2.6 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5416 | 4.0813 | 3.8781 | 3.2545 | 3.4453 | 3.9396 | 5.943 | 7.3177 | 6.468 | 6.6828 | 6.2006 | 6.8447 | 3.5431 | 4.6555 | 3.8698 | 4.9209 | 3.7205 | 4.4373 |
Actin | 0.0242 | 0.0167 | 0.016 | 0.0054 | 0.0044 | 0.0079 | 0.0301 | 0.024 | 0.014 | 0.0023 | 0.0037 | 0.0238 | 0.0283 | 0.0042 | 0.0103 | 0.0011 | 0.0042 | 0.005 |
Bud | 0.0005 | 0.0013 | 0.0091 | 0.0004 | 0.0001 | 0.0038 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0009 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0006 | 0.0005 | 0.0029 | 0.0003 | 0.0002 | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Site | 0.0013 | 0.0067 | 0.0043 | 0.0009 | 0.0002 | 0.0009 | 0.0004 | 0.0003 | 0.0028 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0051 | 0.0018 | 0.0028 | 0.002 | 0.0008 | 0.0091 | 0.0007 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0018 | 0.0005 | 0.0004 | 0.0004 | 0.0006 | 0.0004 |
Cytoplasmic Foci | 0.1118 | 0.157 | 0.1017 | 0.1522 | 0.0911 | 0.1026 | 0.0976 | 0.1181 | 0.0797 | 0.0832 | 0.108 | 0.0754 | 0.1034 | 0.0978 | 0.0872 | 0.1144 | 0.0913 | 0.0737 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.005 | 0.0002 | 0.0004 | 0.0011 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Endosome | 0.1858 | 0.175 | 0.1714 | 0.167 | 0.1968 | 0.1296 | 0.1235 | 0.0841 | 0.1105 | 0.1134 | 0.0762 | 0.0708 | 0.1339 | 0.1566 | 0.1378 | 0.1308 | 0.1573 | 0.1163 |
Golgi | 0.4299 | 0.5498 | 0.5598 | 0.5753 | 0.6064 | 0.5995 | 0.5765 | 0.6876 | 0.6989 | 0.6209 | 0.6303 | 0.6878 | 0.529 | 0.682 | 0.6721 | 0.6127 | 0.5967 | 0.7069 |
Lipid Particles | 0.0528 | 0.0096 | 0.0214 | 0.0281 | 0.0091 | 0.0314 | 0.052 | 0.0064 | 0.0127 | 0.0465 | 0.0499 | 0.0346 | 0.0662 | 0.0257 | 0.0199 | 0.0769 | 0.046 | 0.0275 |
Mitochondria | 0.0562 | 0.0219 | 0.0415 | 0.0475 | 0.0621 | 0.0254 | 0.0224 | 0.0256 | 0.0314 | 0.0266 | 0.0027 | 0.0152 | 0.0331 | 0.0113 | 0.0257 | 0.0428 | 0.0208 | 0.0164 |
None | 0.0116 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0016 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0113 | 0 | 0.0001 | 0 | 0 | 0 |
Nuclear Periphery | 0.0029 | 0.0005 | 0.0007 | 0.0004 | 0.0005 | 0.0025 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleolus | 0.0066 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0936 | 0.0545 | 0.061 | 0.0135 | 0.0141 | 0.0766 | 0.0912 | 0.0523 | 0.0465 | 0.1051 | 0.1282 | 0.0897 | 0.0854 | 0.0191 | 0.0427 | 0.0191 | 0.0793 | 0.0511 |
Punctate Nuclear | 0.0034 | 0.0013 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0023 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0024 | 0.0011 | 0.0029 | 0.0012 | 0.0041 | 0.002 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.001 | 0.0008 | 0.0003 | 0.0006 | 0.0006 |
Vacuole Periphery | 0.0023 | 0.0009 | 0.0025 | 0.0016 | 0.0087 | 0.0015 | 0.001 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0006 | 0.0011 | 0.0011 | 0.0015 | 0.0008 | 0.0022 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.9465 | 78.4428 | 66.258 | 54.0105 | 61.1218 | 56.9124 | 76.7821 | 65.2056 | 62.2769 | 75.09 |
Translational Efficiency | 1.0007 | 0.9141 | 0.776 | 0.9589 | 0.8844 | 0.8679 | 0.8563 | 0.7219 | 0.6569 | 0.661 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1665 | 1236 | 1447 | 1036 | 1747 | 2131 | 1127 | 1051 | 3412 | 3367 | 2574 | 2087 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 671.34 | 856.52 | 915.71 | 885.36 | 773.68 | 815.70 | 917.19 | 860.30 | 723.74 | 830.68 | 916.36 | 872.74 |
Standard Deviation | 81.83 | 124.05 | 114.28 | 173.18 | 95.64 | 316.69 | 106.88 | 168.76 | 102.80 | 263.65 | 111.10 | 171.43 |
Intensity Change Log 2 | — | 0.351443 | 0.447847 | 0.399221 | — | 0.076302 | 0.245484 | 0.153103 | — | 0.210675 | 0.343039 | 0.272683 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000544 | 0.003156 | 0.004448 | 0.022274 | 0.003367 | 0.003381 | 0.004913 | 0.004703 | 0.001990 | 0.003299 | 0.004651 | 0.013425 |
Bud Neck | 0.001767 | 0.000184 | 0.000468 | 0.003503 | 0.000067 | 0.000264 | 0.000098 | 0.003014 | 0.000897 | 0.000235 | 0.000306 | 0.003257 |
Bud Site | 0.000532 | 0.001804 | 0.004646 | 0.040535 | 0.000202 | 0.000834 | 0.001201 | 0.018258 | 0.000363 | 0.001190 | 0.003138 | 0.029316 |
Cell Periphery | 0.000046 | 0.000038 | 0.000043 | 0.000174 | 0.000026 | 0.000048 | 0.000025 | 0.000255 | 0.000036 | 0.000044 | 0.000035 | 0.000215 |
Cytoplasm | 0.003257 | 0.002018 | 0.001966 | 0.026340 | 0.000270 | 0.001350 | 0.001203 | 0.014188 | 0.001728 | 0.001595 | 0.001632 | 0.020220 |
Cytoplasmic Foci | 0.203382* | 0.284370* | 0.387610* | 0.273560* | 0.214942* | 0.211625* | 0.333994* | 0.261023* | 0.209301* | 0.238329* | 0.364135* | 0.267246* |
Eisosomes | 0.000105 | 0.000040 | 0.000033 | 0.000087 | 0.000066 | 0.000052 | 0.000032 | 0.000066 | 0.000085 | 0.000048 | 0.000032 | 0.000076 |
Endoplasmic Reticulum | 0.000071 | 0.000304 | 0.000919 | 0.000524 | 0.000055 | 0.000112 | 0.000379 | 0.001052 | 0.000062 | 0.000183 | 0.000683 | 0.000790 |
Endosome | 0.134197 | 0.153372 | 0.145533 | 0.213460* | 0.225546* | 0.232757* | 0.197330 | 0.318318* | 0.180969 | 0.203616* | 0.168212 | 0.266266* |
Golgi | 0.541142* | 0.502773* | 0.368078* | 0.253259* | 0.506961* | 0.498638* | 0.428472* | 0.216931* | 0.523641* | 0.500156* | 0.394521* | 0.234964* |
Lipid Particles | 0.039690 | 0.012943 | 0.007509 | 0.005683 | 0.013142 | 0.011547 | 0.006280 | 0.016128 | 0.026097 | 0.012059 | 0.006971 | 0.010943 |
Mitochondria | 0.037110 | 0.014876 | 0.004525 | 0.018593 | 0.017227 | 0.021477 | 0.002789 | 0.020018 | 0.026930 | 0.019054 | 0.003765 | 0.019311 |
Mitotic Spindle | 0.003720 | 0.004133 | 0.050679 | 0.049955 | 0.000832 | 0.001149 | 0.006955 | 0.052239 | 0.002241 | 0.002245 | 0.031535 | 0.051105 |
None | 0.000678 | 0.000091 | 0.000022 | 0.001300 | 0.000012 | 0.000394 | 0.000008 | 0.004351 | 0.000337 | 0.000283 | 0.000016 | 0.002837 |
Nuclear Periphery | 0.000017 | 0.000031 | 0.000177 | 0.000233 | 0.000008 | 0.000011 | 0.000039 | 0.000189 | 0.000012 | 0.000019 | 0.000117 | 0.000211 |
Nuclear Periphery Foci | 0.000144 | 0.000429 | 0.002415 | 0.001270 | 0.000040 | 0.000046 | 0.000378 | 0.001803 | 0.000091 | 0.000187 | 0.001523 | 0.001539 |
Nucleolus | 0.000265 | 0.000050 | 0.000020 | 0.000119 | 0.000034 | 0.000012 | 0.000003 | 0.000485 | 0.000147 | 0.000026 | 0.000013 | 0.000303 |
Nucleus | 0.000237 | 0.000315 | 0.000090 | 0.000286 | 0.000019 | 0.002726 | 0.000009 | 0.000525 | 0.000125 | 0.001841 | 0.000055 | 0.000407 |
Peroxisomes | 0.030666 | 0.014568 | 0.007845 | 0.046081 | 0.015372 | 0.010385 | 0.008805 | 0.027160 | 0.022835 | 0.011920 | 0.008265 | 0.036552 |
Vacuole | 0.001288 | 0.002311 | 0.009933 | 0.037203 | 0.000786 | 0.001435 | 0.004814 | 0.031508 | 0.001031 | 0.001757 | 0.007692 | 0.034335 |
Vacuole Periphery | 0.001142 | 0.002192 | 0.003039 | 0.005561 | 0.001026 | 0.001758 | 0.002274 | 0.007785 | 0.001082 | 0.001917 | 0.002704 | 0.006681 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.92 | -5.25 | -7.53 | -6.31 | -5.95 | -0.01 | -1.36 | -1.32 | -1.27 | 0.14 | -2.07 | -3.97 | -7.44 | -6.33 | -5.41 |
Bud Neck | 4.58 | 3.60 | -2.63 | -5.17 | -4.71 | -1.23 | -2.13 | -7.12 | -6.27 | -7.04 | 3.37 | 3.28 | -5.85 | -7.69 | -7.65 |
Bud Site | -1.80 | -6.46 | -11.70 | -11.08 | -10.30 | -1.56 | -5.87 | -7.98 | -7.59 | -7.51 | -2.27 | -7.53 | -14.06 | -13.45 | -12.48 |
Cell Periphery | 0.65 | 0.33 | -3.97 | -4.13 | -4.10 | -1.81 | 0.40 | -3.13 | -2.78 | -3.15 | -1.03 | 0.09 | -4.47 | -4.15 | -4.48 |
Cytoplasm | 1.24 | 1.70 | -9.45 | -9.74 | -9.97 | -2.84 | -1.88 | -8.60 | -7.82 | -7.64 | 0.19 | -0.11 | -12.50 | -12.46 | -12.47 |
Cytoplasmic Foci | -13.41 | -30.07 | -8.88 | 2.34 | 15.50 | 0.68 | -17.97 | -6.57 | -7.24 | 9.38 | -7.62 | -34.22 | -10.92 | -4.89 | 18.12 |
Eisosomes | 8.12 | 9.67 | 1.11 | -3.00 | -3.52 | 2.38 | 6.47 | -0.03 | -1.60 | -4.04 | 7.51 | 11.59 | 0.91 | -3.22 | -5.06 |
Endoplasmic Reticulum | -2.71 | -5.59 | -7.97 | -2.46 | 2.22 | -1.11 | -3.23 | -2.98 | -2.78 | -1.95 | -2.64 | -6.45 | -4.34 | -3.53 | -0.65 |
Endosome | -3.51 | -2.37 | -10.10 | -7.24 | -9.04 | -1.25 | 4.69 | -10.24 | -9.68 | -13.64 | -5.48 | 3.27 | -13.73 | -9.81 | -16.65 |
Golgi | 4.24 | 20.43 | 30.52* | 26.87 | 13.56 | 1.20 | 9.46 | 33.84 | 33.96* | 22.64* | 4.16 | 21.77 | 45.24* | 42.97* | 25.08* |
Lipid Particles | 8.44 | 11.29 | 11.83 | 3.79 | 1.09 | 0.81 | 3.64 | -1.35 | -2.04 | -4.41 | 7.58 | 11.32 | 7.96 | 0.44 | -3.58 |
Mitochondria | 7.05 | 12.04 | 5.79 | -0.96 | -5.48 | -1.71 | 7.92 | -0.78 | 0.67 | -7.02 | 3.90 | 14.10 | 3.73 | 0.22 | -8.70 |
Mitotic Spindle | -0.35 | -11.67 | -10.65 | -10.57 | -0.78 | -0.58 | -3.91 | -10.93 | -10.90 | -9.25 | 0.02 | -12.24 | -15.27 | -15.33 | -5.69 |
None | 2.27 | 2.56 | -0.76 | -1.61 | -1.71 | -2.00 | 1.75 | -3.25 | -2.94 | -3.25 | 0.32 | 2.57 | -3.22 | -3.29 | -3.67 |
Nuclear Periphery | -1.10 | -3.56 | -2.18 | -2.03 | -0.63 | -1.94 | -4.37 | -5.48 | -5.39 | -4.50 | -1.24 | -4.12 | -3.88 | -3.76 | -1.84 |
Nuclear Periphery Foci | -1.67 | -3.60 | -2.99 | -2.19 | 1.15 | -0.66 | -2.89 | -5.49 | -5.47 | -4.28 | -1.51 | -4.02 | -5.79 | -5.33 | -0.57 |
Nucleolus | 2.61 | 2.99 | 1.37 | -3.11 | -4.43 | 0.71 | 1.00 | -4.48 | -4.89 | -4.97 | 2.81 | 3.11 | -2.61 | -5.70 | -5.96 |
Nucleus | -0.27 | 1.93 | -2.38 | -0.69 | -4.18 | -3.00 | 0.22 | -4.12 | 2.23 | -4.16 | -2.95 | 1.72 | -4.77 | 2.13 | -5.62 |
Peroxisomes | 6.78 | 10.66 | -4.03 | -8.54 | -10.74 | 2.91 | 3.13 | -4.72 | -7.40 | -7.19 | 7.65 | 9.91 | -5.99 | -11.48 | -12.98 |
Vacuole | -2.14 | -9.48 | -12.97 | -12.65 | -10.31 | -4.00 | -5.52 | -12.00 | -11.79 | -10.28 | -3.40 | -11.02 | -17.62 | -17.30 | -14.31 |
Vacuole Periphery | -3.05 | -5.78 | -6.65 | -4.47 | -3.26 | -3.76 | -4.91 | -5.97 | -5.27 | -4.75 | -4.73 | -7.55 | -8.46 | -6.97 | -5.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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