Standard name
Human Ortholog
Description Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.07 0 0.08 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.28 0.35 0.33 0.26 0.23 0.13 – 0.05 0.08 0.06 0.06 0.32 0.24 0.21 0.33 0.25 0.19 0.06 0.09 0.06 0.12 0.08 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.4 0.31 0.15 0.14 0.14 0.06 0.27 – 0.2 0.23 0.26 0.2 0.34 0.35 0.45 0.3 0.27 0.29 0.44 0.45 0.39 0.29 0.29 0.34
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.19 0 0.05 0.05 0.3 0.12 – 0.41 0.35 0.46 0.42 0 0 0 0.11 0.09 0.06 0.1 0.12 0.17 0.14 0.11 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.07 0.12 – 0.22 0.26 0.2 0.28 0.06 0 0.05 0 0.11 0.07 0 0 0 0 0 0
Peroxisomes 0.19 0 0 0 0.07 0 0.09 – 0.06 0 0 0 0.08 0.05 0.21 0 0 0.11 0.08 0 0.07 0.09 0.09 0.14
SpindlePole 0.31 0.3 0.52 0.44 0.55 0.37 0.51 – 0.31 0.27 0.19 0.21 0.42 0.51 0.53 0.42 0.59 0.55 0.18 0.18 0.18 0.23 0.26 0.21
Vac/Vac Membrane 0.05 0 0 0 0 0 0 – 0.05 0.05 0.08 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 9 0 0 5 2 – 0 0 2 1 2 0 0 0 0 0 7 6 6 7 8 5
Bud 5 1 5 8 4 16 9 – 8 7 6 6 1 2 3 1 2 4 3 3 2 3 6 6
Bud Neck 1 3 12 6 2 4 4 – 0 0 2 0 0 9 1 1 5 0 2 2 2 1 1 2
Bud Site 1 0 1 0 0 6 1 – 3 6 5 8 0 1 0 0 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 2 3 0 0 0 0 0 0 0
Cytoplasm 39 46 67 37 33 46 44 – 14 26 18 19 39 44 41 101 48 37 14 18 15 25 19 19
Endoplasmic Reticulum 1 2 1 4 0 2 0 – 2 1 1 3 4 2 1 15 5 1 3 0 1 1 4 5
Endosome 80 50 28 16 18 12 93 – 53 72 76 60 41 64 90 92 51 56 108 92 93 60 73 88
Golgi 1 1 1 0 0 0 1 – 0 0 2 0 0 0 0 0 0 0 6 2 4 1 6 3
Mitochondria 44 30 2 6 6 60 41 – 109 108 136 128 3 6 3 32 17 11 25 24 41 27 27 20
Nucleus 0 0 0 0 1 4 8 – 3 7 4 9 0 0 0 7 4 1 0 1 0 1 0 1
Nuclear Periphery 2 5 0 0 0 0 5 – 9 11 11 17 2 0 1 3 0 0 1 1 1 1 2 2
Nucleolus 10 3 1 2 3 15 43 – 57 82 58 84 7 3 9 13 21 13 1 3 1 0 0 1
Peroxisomes 37 6 1 2 9 6 31 – 17 12 3 9 10 10 42 8 7 20 20 6 16 17 21 34
SpindlePole 61 48 100 49 69 74 178 – 81 85 56 64 51 93 105 126 112 104 43 37 42 45 65 53
Vac/Vac Membrane 9 7 4 3 5 7 9 – 14 16 25 12 2 0 1 11 3 13 2 3 1 2 11 5
Unique Cell Count 199 162 191 111 125 202 347 265 313 295 304 122 182 198 302 189 190 248 208 239 204 256 258
Labelled Cell Count 291 203 232 133 150 258 469 370 433 405 420 162 234 297 412 278 263 248 208 239 204 256 258


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.6 5.0 5.3 6.0 4.4 5.5 – 5.6 6.0 6.0 6.2 5.4 4.7 5.5 6.6 6.8 6.3 4.4 5.4 5.1
Std Deviation (1e-4) 0.8 1.0 1.3 2.1 2.1 1.5 1.4 – 1.3 1.6 1.5 1.5 1.7 0.9 1.7 1.9 1.9 1.5 1.5 2.4 1.8
Intensity Change (Log2) – – – 0.07 0.27 -0.18 0.12 – 0.15 0.25 0.26 0.31 0.11 -0.08 0.13 0.39 0.43 0.32 -0.18 0.1 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 1.0 – 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4646 0.1999 -0.0729 -0.4098 -0.1163 -0.0984 1.6111 1.8702 0.9201 1.7812 0.7164 0.9522 3.199 4.253 3.6281 4.1481 4.0932 3.7786
Actin 0.0357 0.0003 0.0119 0.0102 0.0313 0.0024 0.0194 0.0008 0.0001 0.0024 0.0017 0.0005 0.0019 0.0022 0.0057 0.018 0.0014 0.0023
Bud 0.0003 0 0.0011 0.0009 0.0006 0 0.0015 0.0052 0 0 0.0008 0.0005 0.0007 0.0003 0.0011 0.001 0.0001 0
Bud Neck 0.0007 0.0003 0.0038 0.0003 0.0021 0.0002 0.0035 0.0015 0.0002 0.0002 0.0004 0.0005 0.0003 0.0031 0.0083 0.0017 0.0003 0.0009
Bud Periphery 0.0006 0 0.0037 0.0022 0.0009 0.0001 0.0014 0.0041 0 0.0001 0.0011 0.0002 0.0018 0.0006 0.0027 0.0011 0.0001 0.0001
Bud Site 0.0015 0.0004 0.0049 0.0013 0.0014 0.0001 0.0093 0.0108 0.0002 0.0002 0.0009 0.0003 0.0004 0.005 0.0016 0.0088 0.0002 0.0003
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0005 0.0002 0 0.0001 0.0002 0.0001 0.0004 0.0005 0.0002 0.0002 0 0.0001
Cytoplasm 0.0089 0.0063 0.0039 0.0016 0.0043 0.0055 0.002 0.013 0.0085 0.003 0.0476 0.0262 0.004 0.0187 0.0057 0.0071 0.002 0.0059
Cytoplasmic Foci 0.3022 0.2387 0.2862 0.2219 0.2277 0.2084 0.2513 0.3151 0.3765 0.2862 0.1432 0.2681 0.2712 0.2028 0.2403 0.2436 0.284 0.2485
Eisosomes 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0014 0.0001 0 0.0001 0.0001 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0045 0.001 0.0009 0.0012 0.004 0.0021 0.0007 0.0068 0.001 0.0034 0.0028 0.0021 0.0018 0.005 0.0029 0.0055 0.0002 0.0006
Endosome 0.0734 0.0828 0.0766 0.0572 0.0963 0.0379 0.0822 0.0551 0.0607 0.1158 0.1878 0.0503 0.0445 0.057 0.0562 0.062 0.0285 0.0272
Golgi 0.0188 0.0135 0.0142 0.0131 0.044 0.0111 0.0322 0.0127 0.013 0.037 0.0379 0.0119 0.0083 0.0168 0.015 0.0186 0.0386 0.0101
Lipid Particles 0.3981 0.5403 0.4594 0.5466 0.4687 0.5882 0.3267 0.3802 0.3847 0.3599 0.4205 0.4324 0.5163 0.4622 0.481 0.5054 0.4423 0.578
Mitochondria 0.008 0.0006 0.0023 0.0265 0.0244 0.0026 0.0312 0.0083 0.0004 0.0046 0.0206 0.0253 0.0013 0.0019 0.0034 0.0073 0.006 0.0082
None 0.0759 0.0304 0.0146 0.0012 0.0436 0.0086 0.0023 0.0341 0.0157 0.0302 0.0676 0.0141 0.0549 0.0425 0.0201 0.0063 0.0021 0.0057
Nuclear Periphery 0.0049 0.0013 0.0012 0.0019 0.003 0.0035 0.0013 0.0041 0.003 0.0022 0.0012 0.0015 0.018 0.0026 0.0037 0.0085 0.002 0.0008
Nucleolus 0.0007 0.0027 0.0032 0.0007 0.0003 0.0007 0.0173 0.0064 0.0016 0.0005 0.0006 0.0015 0.0026 0.0078 0.0029 0.0016 0.0008 0.0022
Nucleus 0.0007 0.0004 0.0006 0.0007 0.0004 0.0006 0.0092 0.0018 0.0004 0.0002 0.0007 0.0019 0.0041 0.002 0.0048 0.0034 0.0011 0.0004
Peroxisomes 0.0406 0.0327 0.0704 0.092 0.0232 0.0766 0.1683 0.0728 0.1005 0.0761 0.051 0.1102 0.0352 0.0811 0.0855 0.0761 0.0941 0.0673
Punctate Nuclear 0.0182 0.0456 0.0369 0.0177 0.0188 0.0502 0.0233 0.0643 0.0309 0.0748 0.0053 0.0475 0.027 0.0837 0.0566 0.021 0.0953 0.0389
Vacuole 0.0035 0.0021 0.003 0.0016 0.0023 0.0008 0.013 0.0022 0.0019 0.0012 0.0053 0.0035 0.0041 0.0033 0.0018 0.002 0.0004 0.0023
Vacuole Periphery 0.0022 0.0003 0.0011 0.0009 0.0019 0.0003 0.0031 0.0004 0.0005 0.0006 0.0027 0.0012 0.0011 0.0008 0.0004 0.0007 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.481 15.3251 9.3042 14.7017 12.1238 14.0186 20.4254 10.9336 13.3996 18.0627
Translational Efficiency 0.7995 0.8373 0.9733 0.5798 0.9461 1.0786 0.9115 0.771 0.8354 0.6483

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1350 751 88 1521 1117 2497 2137 1141 2467 3248 2225 2662

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 680.53 738.83 1008.96 853.09 691.14 761.36 920.12 876.96 685.33 756.15 923.63 863.32
Standard Deviation 85.42 99.91 110.27 132.77 73.86 98.72 111.41 124.98 80.56 99.45 112.71 130.02
Intensity Change Log 2 — 0.118584 0.568138 0.326039 — 0.139601 0.412844 0.343533 — 0.129212 0.491980 0.334880

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001152 0.001694 0.001902 0.023538 0.000582 0.000967 0.019580 0.013916 0.000894 0.001135 0.018880 0.019414
Bud Neck 0.004856 0.006882 0.018184 0.017070 0.005712 0.013042 0.008900 0.014188 0.005244 0.011617 0.009267 0.015835
Bud Site 0.001530 0.004856 0.030513 0.057178 0.000822 0.005493 0.016210 0.056728 0.001209 0.005345 0.016776 0.056985
Cell Periphery 0.000105 0.000103 0.000293 0.000275 0.000107 0.000104 0.000135 0.000218 0.000106 0.000104 0.000141 0.000250
Cytoplasm 0.037613 0.043823 0.052835 0.108295* 0.072028 0.050590 0.024212 0.148588* 0.053195 0.049025 0.025344 0.125566*
Cytoplasmic Foci 0.268137* 0.295424* 0.113182 0.159740 0.272186* 0.275725* 0.204452* 0.164833 0.269970* 0.280280* 0.200842* 0.161923
Eisosomes 0.000203 0.000103 0.000107 0.000190 0.000161 0.000095 0.000092 0.000151 0.000184 0.000097 0.000093 0.000174
Endoplasmic Reticulum 0.000951 0.001798 0.008710 0.002736 0.001378 0.001131 0.003321 0.002279 0.001144 0.001285 0.003534 0.002540
Endosome 0.053463 0.161460 0.129146 0.069646 0.064736 0.121572 0.102755 0.066388 0.058567 0.130795 0.103799 0.068250
Golgi 0.043311 0.046737 0.001472 0.008781 0.020273 0.027434 0.007989 0.005342 0.032880 0.031897 0.007731 0.007307
Lipid Particles 0.460355* 0.251792* 0.057509 0.031109 0.397318* 0.322741* 0.179585* 0.035429 0.431813* 0.306336* 0.174757* 0.032961
Mitochondria 0.011334 0.008334 0.000587 0.004826 0.008134 0.016820 0.001574 0.003441 0.009885 0.014858 0.001535 0.004232
Mitotic Spindle 0.012147 0.055655 0.078171 0.196591* 0.028677 0.046459 0.166441* 0.164509* 0.019632 0.048585 0.162949* 0.182840*
None 0.002636 0.001949 0.001472 0.000803 0.002149 0.004089 0.000537 0.001230 0.002415 0.003594 0.000574 0.000986
Nuclear Periphery 0.000633 0.001044 0.003558 0.001361 0.001956 0.001683 0.001817 0.001508 0.001232 0.001535 0.001885 0.001424
Nuclear Periphery Foci 0.008330 0.019118 0.227240* 0.012203 0.017817 0.017830 0.046187 0.016454 0.012626 0.018128 0.053348 0.014025
Nucleolus 0.003646 0.004277 0.018899 0.001184 0.007294 0.009289 0.002095 0.001047 0.005298 0.008130 0.002759 0.001125
Nucleus 0.013619 0.013532 0.152654* 0.036662 0.041161 0.019108 0.015905 0.055702 0.026089 0.017819 0.021314 0.044823
Peroxisomes 0.070956 0.061770 0.042527 0.185898* 0.049229 0.054927 0.176951* 0.143336* 0.061118 0.056509 0.171635* 0.167655*
Vacuole 0.004231 0.018029 0.060344 0.079287 0.007549 0.009710 0.021062 0.099960 0.005733 0.011634 0.022616 0.088148
Vacuole Periphery 0.000794 0.001621 0.000694 0.002628 0.000731 0.001192 0.000201 0.004752 0.000765 0.001291 0.000221 0.003538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.38 -1.71 -8.80 -8.34 -7.71 -1.00 -10.39 -5.63 -5.36 3.63 -0.84 -10.28 -10.44 -10.13 1.85
Bud Neck -1.51 -3.16 -10.08 -7.26 -0.30 -5.71 -5.63 -6.72 -1.66 -2.60 -6.47 -7.43 -12.07 -5.15 -4.93
Bud Site -5.03 -2.67 -14.93 -13.60 -1.24 -6.01 -14.33 -12.96 -11.47 -7.66 -6.61 -13.93 -19.79 -17.58 -10.89
Cell Periphery -0.25 -4.77 -4.94 -4.96 1.37 0.16 -4.09 -4.28 -4.73 -1.71 0.07 -5.76 -6.36 -6.54 -3.09
Cytoplasm -1.65 -1.46 -15.46 -12.30 -5.45 3.61 6.81 -11.88 -16.01 -18.54 0.76 4.81 -18.95 -20.20 -23.34
Cytoplasmic Foci -1.76 11.09 16.51 17.20 -3.36 -0.06 11.30 15.51 19.67 6.79 -0.99 14.97 22.67 26.83 7.84
Eisosomes 6.32 3.77 0.90 -2.67 -1.67 3.49 3.00 0.56 -5.22 -4.19 7.32 6.45 1.00 -4.31 -3.77
Endoplasmic Reticulum -2.83 -4.86 -6.16 -3.34 3.83 0.95 -8.70 -6.82 -6.75 4.35 -0.74 -10.51 -8.37 -6.81 4.30
Endosome -14.76 -2.93 -0.69 14.15 2.77 -11.26 -6.22 2.72 14.58 9.46 -18.77 -9.46 1.39 19.85 10.56
Golgi -0.79 18.43 15.58 12.23 -4.55 -3.69 8.21 10.44 17.32 5.61 0.63 17.39 18.33 19.82 3.10
Lipid Particles 14.26 27.18 43.27* 20.27 1.50 5.84 19.77 33.90 41.31* 23.58* 13.71 31.19 54.65 46.76* 24.86
Mitochondria 1.56 8.21 5.41 3.05 -5.40 -5.09 5.39 4.53 10.48 -1.59 -3.61 8.97 6.98 10.47 -3.48
Mitotic Spindle -7.68 -2.88 -20.49 -12.27 -3.73 -4.63 -21.29* -13.49 -11.41 2.82 -10.10 -24.26 -24.25 -18.21 1.13
None 0.78 2.40 2.92 1.72 -0.13 -2.23 2.93 1.71 4.28 -3.07 -1.75 4.51 3.36 4.85 -3.35
Nuclear Periphery -1.55 -4.69 -7.93 -4.38 2.43 1.07 -3.38 -2.68 -3.86 0.69 -2.08 -7.23 -7.31 -5.14 1.69
Nuclear Periphery Foci -3.96 -6.97 -4.20 1.01 6.70 0.64 -9.23 1.50 1.11 10.40 -3.21 -13.80 -1.53 1.51 12.77
Nucleolus -0.59 -1.44 2.01 2.56 1.66 -1.12 3.53 5.66 9.05 4.13 -2.93 1.94 5.69 8.98 3.75
Nucleus -0.60 -4.92 -10.99 -9.55 3.60 5.41 2.60 -5.74 -11.35 -8.63 3.11 -2.77 -11.15 -14.60 -7.21
Peroxisomes 1.67 4.27 -8.81 -9.59 -9.94 -1.51 -17.83 -7.72 -7.21 6.96 1.63 -16.06 -11.77 -13.29 4.81
Vacuole -6.64 -5.91 -26.32 -22.01 -3.66 -2.25 -13.76 -24.26 -23.95 -18.74 -7.26 -15.87 -35.74 -34.00 -24.65
Vacuole Periphery -2.78 0.58 -1.42 0.03 -1.74 -2.32 4.14 -2.30 -1.78 -2.86 -2.86 4.05 -2.68 -1.64 -3.80
Standard name
Human Ortholog
Description Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Bsc2

Bsc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bsc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available