Standard name | |
---|---|
Human Ortholog | |
Description | Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 14 | 18 | 21 | 15 | 20 | 23 | – | 23 | 13 | 28 | 8 | 9 | 10 | 8 | 7 | 12 | 0 | 2 | 4 | 3 | 2 | 4 |
Bud | 0 | 0 | 0 | 2 | 7 | 14 | 6 | 19 | – | 13 | 14 | 23 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 2 | 6 | 17 | 19 | 21 | 27 | 17 | – | 26 | 22 | 43 | 21 | 24 | 51 | 43 | 33 | 32 | 0 | 1 | 1 | 0 | 2 | 4 |
Cytoplasm | 20 | 11 | 17 | 36 | 58 | 75 | 81 | 117 | – | 124 | 102 | 131 | 21 | 20 | 22 | 23 | 26 | 28 | 1 | 2 | 1 | 0 | 6 | 5 |
Endoplasmic Reticulum | 97 | 54 | 198 | 138 | 203 | 228 | 198 | 149 | – | 168 | 72 | 239 | 192 | 210 | 184 | 2 | 1 | 3 | 228 | 82 | 232 | 161 | 195 | 227 |
Endosome | 1 | 0 | 0 | 5 | 10 | 1 | 5 | 7 | – | 6 | 8 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Golgi | 5 | 0 | 1 | 6 | 7 | 1 | 3 | 0 | – | 3 | 2 | 0 | 1 | 6 | 1 | 24 | 17 | 19 | 0 | 0 | 1 | 0 | 2 | 2 |
Mitochondria | 4 | 1 | 18 | 59 | 52 | 69 | 159 | 121 | – | 109 | 156 | 234 | 0 | 0 | 1 | 9 | 10 | 6 | 2 | 0 | 7 | 4 | 2 | 4 |
Nucleus | 1 | 0 | 1 | 0 | 4 | 3 | 6 | 7 | – | 7 | 4 | 2 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 1 | 35 | 15 | 13 | 12 | 28 | 20 | – | 25 | 6 | 39 | 4 | 3 | 5 | 0 | 5 | 1 | 7 | 1 | 6 | 5 | 3 | 2 |
Nucleolus | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | – | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 43 | 3 | 11 | 40 | 124 | 77 | 178 | 216 | – | 165 | 141 | 135 | 19 | 13 | 17 | 7 | 9 | 14 | 2 | 0 | 1 | 8 | 7 | 11 |
Unique Cell Count | 159 | 67 | 228 | 263 | 418 | 418 | 558 | 578 | 546 | 461 | 703 | 222 | 252 | 241 | 105 | 95 | 101 | 249 | 94 | 258 | 190 | 232 | 272 | |
Labelled Cell Count | 185 | 73 | 301 | 337 | 518 | 516 | 713 | 698 | 672 | 545 | 880 | 268 | 291 | 298 | 117 | 108 | 116 | 249 | 94 | 258 | 190 | 232 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.7 | 14.1 | 9.5 | 10.2 | 10.9 | 10.4 | 9.5 | 9.7 | – | 9.0 | 9.1 | 8.8 | 14.5 | 13.2 | 12.7 | 19.0 | 20.0 | 20.4 | 14.5 | 14.9 | 14.4 |
Std Deviation (1e-4) | 6.2 | 4.0 | 2.4 | 2.4 | 2.6 | 2.4 | 2.4 | 2.1 | – | 2.3 | 2.2 | 2.0 | 3.0 | 3.3 | 2.9 | 4.6 | 4.5 | 5.4 | 3.1 | 3.4 | 3.2 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.19 | 0.12 | -0.0 | 0.03 | – | -0.09 | -0.08 | -0.12 | 0.6 | 0.47 | 0.41 | 0.99 | 1.07 | 1.1 | 0.6 | 0.65 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | -0.6 | -1.5 | -1.6 | -1.3 | – | -1.1 | -2.1 | -1.4 | -1.2 | -1.3 | -1.0 | 0.5 | 0.4 | 1.8 |
Bud | 0 | 0 | 0 | 0 | 2.8 | – | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.0 | 1.2 | 1.5 | 1.4 | 0.2 | – | 1.4 | 1.3 | 2.0 | 3.0 | 3.1 | 6.1 | 9.2 | 8.1 | 7.6 |
Cytoplasm | 2.2 | 2.4 | 3.6 | 2.7 | 4.4 | – | 5.0 | 4.8 | 4.0 | 0.8 | 0.2 | 0.7 | 3.8 | 4.8 | 4.9 |
Endoplasmic Reticulum | -8.2 | -9.6 | -8.3 | -13.1 | -15.8 | – | -14.2 | -18.0 | -13.9 | -0.1 | -1.1 | -2.9 | -14.7 | -14.4 | -14.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 7.2 | 6.2 | 6.4 |
Mitochondria | 4.4 | 1.8 | 3.1 | 6.3 | 4.4 | – | 4.1 | 7.4 | 7.5 | -4.3 | -4.5 | -4.1 | 0.2 | 0.8 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -3.5 | -5.7 | -5.8 | -4.8 | -6.0 | – | -5.1 | -7.3 | -4.8 | -5.1 | -5.7 | -5.1 | -4.2 | -2.5 | -3.8 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.8 | 7.4 | 4.8 | 8.1 | 9.3 | – | 7.7 | 7.7 | 5.2 | 1.6 | 0.2 | 1.0 | 0.7 | 1.6 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1233 | 8.7279 | 9.0223 | 6.0302 | 7.7175 | 8.0239 | 10.1247 | 12.9079 | 12.0196 | 10.6782 | 8.9904 | 11.0424 | 7.8062 | 9.327 | 10.0423 | 8.2171 | 8.3668 | 8.9623 |
Actin | 0.0092 | 0.001 | 0.0104 | 0.004 | 0.003 | 0.0013 | 0.0118 | 0.0003 | 0.0025 | 0.0002 | 0.001 | 0.0001 | 0.0076 | 0.0001 | 0.0001 | 0.002 | 0.0045 | 0.0017 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0.0027 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 |
Bud Neck | 0.0005 | 0.0011 | 0.0003 | 0.0002 | 0.0008 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0016 |
Bud Periphery | 0.0005 | 0.0001 | 0.0009 | 0.0036 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0009 | 0 | 0 | 0 | 0.0005 | 0.0001 |
Bud Site | 0.0057 | 0.0038 | 0.0012 | 0.0007 | 0.0016 | 0.0002 | 0.0006 | 0.0007 | 0.0053 | 0.0001 | 0.0002 | 0 | 0.0041 | 0.0006 | 0 | 0.0002 | 0.0005 | 0 |
Cell Periphery | 0.0009 | 0.0039 | 0.0028 | 0.0009 | 0.001 | 0.0034 | 0.0007 | 0.0025 | 0.0017 | 0.0032 | 0.0018 | 0.0003 | 0.0011 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0.0005 |
Cytoplasm | 0.0249 | 0.0674 | 0.0249 | 0.0115 | 0.0173 | 0.0523 | 0.0122 | 0.0129 | 0.0094 | 0.0026 | 0.0157 | 0.0057 | 0.0116 | 0.0302 | 0.0052 | 0.0094 | 0.0274 | 0.0115 |
Cytoplasmic Foci | 0.0105 | 0.002 | 0.0074 | 0.0082 | 0.0106 | 0.0077 | 0.0064 | 0.0007 | 0.0035 | 0.0034 | 0.0027 | 0.0003 | 0.0039 | 0.001 | 0.0002 | 0.0114 | 0.004 | 0.0003 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.8089 | 0.8643 | 0.8804 | 0.7969 | 0.7663 | 0.8549 | 0.8724 | 0.947 | 0.9374 | 0.93 | 0.8916 | 0.9755 | 0.8831 | 0.9449 | 0.9856 | 0.9259 | 0.801 | 0.9657 |
Endosome | 0.0295 | 0.0102 | 0.0177 | 0.0453 | 0.0779 | 0.0181 | 0.0218 | 0.0045 | 0.0083 | 0.029 | 0.0182 | 0.0017 | 0.0103 | 0.0023 | 0.0013 | 0.0116 | 0.0435 | 0.0019 |
Golgi | 0.0093 | 0.0019 | 0.003 | 0.0169 | 0.0104 | 0.0066 | 0.007 | 0.0005 | 0.0066 | 0.0042 | 0.0151 | 0.0003 | 0.0031 | 0.0002 | 0.0003 | 0.0039 | 0.0139 | 0.0008 |
Lipid Particles | 0.0108 | 0.0089 | 0.009 | 0.0059 | 0.0227 | 0.0079 | 0.0164 | 0.0072 | 0.0053 | 0.0062 | 0.018 | 0.0017 | 0.0044 | 0.0036 | 0.0012 | 0.0127 | 0.0236 | 0.0014 |
Mitochondria | 0.0152 | 0.0009 | 0.0074 | 0.0568 | 0.0047 | 0.0045 | 0.0057 | 0.0003 | 0.0054 | 0.0007 | 0.012 | 0.0001 | 0.002 | 0.0001 | 0 | 0.0006 | 0.0509 | 0.0001 |
None | 0.003 | 0.0005 | 0.0059 | 0.0012 | 0.0037 | 0.0017 | 0.0036 | 0.0002 | 0.0003 | 0.0002 | 0.0018 | 0.0006 | 0.0098 | 0.0001 | 0 | 0.0005 | 0.001 | 0.0001 |
Nuclear Periphery | 0.0458 | 0.0226 | 0.0166 | 0.022 | 0.0459 | 0.0211 | 0.0258 | 0.0148 | 0.0049 | 0.005 | 0.0104 | 0.011 | 0.0375 | 0.0101 | 0.0041 | 0.0138 | 0.0131 | 0.0108 |
Nucleolus | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0021 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0 |
Nucleus | 0.0111 | 0.0025 | 0.0017 | 0.0024 | 0.0052 | 0.0045 | 0.0023 | 0.0013 | 0.0003 | 0.0003 | 0.0013 | 0.0004 | 0.0026 | 0.001 | 0.0001 | 0.0013 | 0.0037 | 0.001 |
Peroxisomes | 0.0039 | 0.0001 | 0.0006 | 0.0023 | 0.0032 | 0.0015 | 0.0032 | 0 | 0.0042 | 0.0002 | 0.0003 | 0 | 0.0058 | 0 | 0 | 0.0022 | 0.0025 | 0 |
Punctate Nuclear | 0.0027 | 0.0004 | 0.0017 | 0.0048 | 0.0055 | 0.0042 | 0.0043 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0 | 0.001 | 0.0021 | 0.0001 |
Vacuole | 0.004 | 0.0076 | 0.0066 | 0.0098 | 0.0125 | 0.0063 | 0.003 | 0.0053 | 0.0037 | 0.0133 | 0.0062 | 0.0018 | 0.0078 | 0.0035 | 0.0012 | 0.0029 | 0.0046 | 0.0022 |
Vacuole Periphery | 0.0021 | 0.0007 | 0.0011 | 0.0039 | 0.0048 | 0.0014 | 0.0016 | 0.0012 | 0.0006 | 0.001 | 0.0021 | 0.0003 | 0.0016 | 0.0003 | 0.0001 | 0.0005 | 0.0017 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 157.1038 | 101.4558 | 50.8868 | 55.5134 | 115.8115 | 115.5308 | 88.8093 | 56.7349 | 61.9074 | 120.7888 |
Translational Efficiency | 1.1624 | 1.2535 | 1.2121 | 1.2886 | 1.2819 | 1.1302 | 0.9672 | 1.0703 | 0.9295 | 0.9936 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1273 | 1216 | 733 | 1135 | 1504 | 776 | 1702 | 1478 | 2777 | 1992 | 2435 | 2613 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1218.35 | 1243.59 | 1615.63 | 2149.55 | 1198.33 | 1177.24 | 1701.67 | 1876.13 | 1207.51 | 1217.74 | 1675.77 | 1994.89 |
Standard Deviation | 221.61 | 271.03 | 263.14 | 455.02 | 289.58 | 238.10 | 307.43 | 399.48 | 260.82 | 260.72 | 297.43 | 445.61 |
Intensity Change Log 2 | — | 0.029582 | 0.407168 | 0.819106 | — | -0.025617 | 0.505926 | 0.646735 | — | 0.002475 | 0.456983 | 0.736206 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000610 | 0.001962 | 0.000939 | 0.000899 | 0.001175 | 0.001318 | 0.001328 | 0.000797 | 0.000916 | 0.001711 | 0.001211 | 0.000841 |
Bud Neck | 0.000881 | 0.000413 | 0.002900 | 0.002452 | 0.000414 | 0.000556 | 0.000847 | 0.001628 | 0.000628 | 0.000469 | 0.001466 | 0.001986 |
Bud Site | 0.004048 | 0.005281 | 0.005069 | 0.008193 | 0.005793 | 0.007193 | 0.003529 | 0.007941 | 0.004993 | 0.006026 | 0.003993 | 0.008051 |
Cell Periphery | 0.001858 | 0.001791 | 0.002445 | 0.018631 | 0.002345 | 0.003260 | 0.003014 | 0.010929 | 0.002121 | 0.002363 | 0.002843 | 0.014274 |
Cytoplasm | 0.004145 | 0.013814 | 0.010629 | 0.004674 | 0.007544 | 0.012621 | 0.002363 | 0.004083 | 0.005986 | 0.013349 | 0.004851 | 0.004340 |
Cytoplasmic Foci | 0.002123 | 0.005717 | 0.000906 | 0.001812 | 0.011276 | 0.005242 | 0.000379 | 0.001633 | 0.007080 | 0.005532 | 0.000537 | 0.001710 |
Eisosomes | 0.000019 | 0.000035 | 0.000041 | 0.000204 | 0.000032 | 0.000076 | 0.000070 | 0.000106 | 0.000026 | 0.000051 | 0.000061 | 0.000149 |
Endoplasmic Reticulum | 0.964995* | 0.898647* | 0.884916* | 0.885663* | 0.908128* | 0.928044* | 0.972344* | 0.931542* | 0.934196* | 0.910099* | 0.946026* | 0.911613* |
Endosome | 0.004673 | 0.017466 | 0.012514 | 0.009465 | 0.015198 | 0.009407 | 0.003821 | 0.007004 | 0.010373 | 0.014326 | 0.006438 | 0.008073 |
Golgi | 0.001469 | 0.006132 | 0.000885 | 0.008379 | 0.008178 | 0.003779 | 0.000622 | 0.004456 | 0.005103 | 0.005215 | 0.000701 | 0.006160 |
Lipid Particles | 0.001050 | 0.009454 | 0.008355 | 0.018024 | 0.005253 | 0.003731 | 0.002562 | 0.009013 | 0.003326 | 0.007224 | 0.004306 | 0.012927 |
Mitochondria | 0.001230 | 0.001233 | 0.000178 | 0.001535 | 0.001803 | 0.002894 | 0.000215 | 0.001345 | 0.001540 | 0.001880 | 0.000204 | 0.001427 |
Mitotic Spindle | 0.000113 | 0.000152 | 0.000503 | 0.001664 | 0.000170 | 0.000391 | 0.000116 | 0.000794 | 0.000144 | 0.000246 | 0.000232 | 0.001172 |
None | 0.001576 | 0.003871 | 0.000186 | 0.001875 | 0.002743 | 0.000945 | 0.000181 | 0.001030 | 0.002208 | 0.002731 | 0.000183 | 0.001397 |
Nuclear Periphery | 0.000905 | 0.003164 | 0.018723 | 0.002888 | 0.002260 | 0.001442 | 0.001235 | 0.000955 | 0.001639 | 0.002493 | 0.006499 | 0.001795 |
Nuclear Periphery Foci | 0.004379 | 0.019302 | 0.037444 | 0.010739 | 0.013227 | 0.008719 | 0.003536 | 0.003928 | 0.009171 | 0.015179 | 0.013743 | 0.006886 |
Nucleolus | 0.000111 | 0.000095 | 0.000009 | 0.000024 | 0.000176 | 0.000045 | 0.000003 | 0.000015 | 0.000147 | 0.000075 | 0.000004 | 0.000018 |
Nucleus | 0.001730 | 0.000610 | 0.001002 | 0.000476 | 0.001150 | 0.000961 | 0.000281 | 0.000294 | 0.001416 | 0.000747 | 0.000498 | 0.000373 |
Peroxisomes | 0.000127 | 0.000398 | 0.000018 | 0.000043 | 0.000446 | 0.000367 | 0.000048 | 0.000156 | 0.000300 | 0.000386 | 0.000039 | 0.000107 |
Vacuole | 0.003153 | 0.007925 | 0.011863 | 0.018781 | 0.008007 | 0.005756 | 0.003259 | 0.009699 | 0.005782 | 0.007080 | 0.005849 | 0.013644 |
Vacuole Periphery | 0.000805 | 0.002539 | 0.000475 | 0.003579 | 0.004679 | 0.003255 | 0.000248 | 0.002655 | 0.002904 | 0.002818 | 0.000316 | 0.003056 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.75 | -0.99 | -1.34 | 1.12 | -0.01 | -0.25 | -0.35 | 0.65 | 0.94 | 1.02 | -1.48 | -0.91 | -0.20 | 1.46 | 0.86 |
Bud Neck | 1.92 | -3.44 | -3.45 | -4.97 | 0.78 | -1.09 | -2.17 | -3.92 | -3.45 | -2.21 | 1.30 | -3.59 | -5.14 | -6.02 | -1.55 |
Bud Site | -0.89 | -0.83 | -2.60 | -2.08 | -1.52 | -0.76 | 1.55 | -1.80 | -0.82 | -3.05 | -0.95 | 0.63 | -3.08 | -2.20 | -3.52 |
Cell Periphery | 0.22 | -1.48 | -8.20 | -8.28 | -7.86 | -0.84 | -1.03 | -6.66 | -4.79 | -5.63 | -0.48 | -1.55 | -10.55 | -9.91 | -9.35 |
Cytoplasm | -5.18 | -3.91 | -0.82 | 3.98 | 2.92 | -2.32 | 5.04 | 2.43 | 3.68 | -1.79 | -5.15 | 0.74 | 1.19 | 5.65 | 0.52 |
Cytoplasmic Foci | -3.08 | 1.85 | 0.47 | 3.40 | -1.30 | 3.42 | 7.67 | 6.34 | 3.03 | -2.24 | 1.41 | 7.96 | 5.99 | 4.55 | -2.81 |
Eisosomes | -2.68 | -2.32 | -4.54 | -4.15 | -3.93 | -2.44 | -1.83 | -2.96 | -1.09 | -1.01 | -3.16 | -2.34 | -5.40 | -4.17 | -3.11 |
Endoplasmic Reticulum | 9.38 | 9.80 | 10.36 | 2.01 | -0.14 | -2.35 | -10.39 | -2.66 | 0.03 | 7.54 | 4.29 | -1.94 | 4.92 | 0.49 | 6.84 |
Endosome | -10.63 | -7.33 | -6.01 | 6.20 | 2.79 | 4.29 | 11.57 | 7.34 | 1.91 | -4.60 | -4.17 | 5.39 | 2.92 | 6.83 | -2.73 |
Golgi | -4.57 | 1.26 | -4.85 | -1.36 | -5.47 | 3.28 | 6.52 | 2.84 | -0.77 | -6.10 | -0.13 | 6.60 | -1.14 | -1.03 | -7.85 |
Lipid Particles | -5.24 | -4.55 | -7.89 | -3.33 | -3.29 | 1.49 | 2.36 | -2.77 | -4.09 | -4.66 | -3.57 | -1.52 | -7.87 | -3.92 | -6.21 |
Mitochondria | -0.01 | 2.44 | -0.41 | -0.44 | -1.69 | -1.14 | 5.24 | 0.72 | 1.45 | -2.30 | -0.74 | 5.18 | 0.10 | 0.65 | -2.73 |
Mitotic Spindle | -0.89 | -2.67 | -2.22 | -2.17 | -1.46 | -1.78 | 0.57 | -2.98 | -1.84 | -3.07 | -1.82 | -1.58 | -3.22 | -2.89 | -2.77 |
None | -1.94 | 3.07 | -0.34 | 1.35 | -1.87 | 2.02 | 3.02 | 1.65 | -0.13 | -1.40 | -0.61 | 4.03 | 1.08 | 1.53 | -2.33 |
Nuclear Periphery | -3.11 | -7.11 | -6.10 | 0.26 | 6.27 | 1.88 | 2.47 | 3.43 | 1.66 | 1.29 | -1.74 | -6.03 | -0.82 | 1.40 | 5.90 |
Nuclear Periphery Foci | -7.13 | -10.48 | -4.07 | 3.38 | 7.93 | 2.12 | 5.96 | 5.30 | 2.62 | -0.61 | -3.74 | -3.49 | 1.56 | 5.25 | 5.25 |
Nucleolus | 0.34 | 2.80 | 2.38 | 1.61 | -2.95 | 1.45 | 1.86 | 1.74 | 2.05 | -5.86 | 1.29 | 2.60 | 2.35 | 2.08 | -6.03 |
Nucleus | 1.81 | 0.84 | 1.85 | 0.12 | 2.16 | 0.31 | 1.35 | 1.45 | 2.13 | 0.17 | 1.57 | 1.85 | 2.34 | 1.98 | 1.14 |
Peroxisomes | -1.16 | 4.22 | 3.16 | 1.52 | -2.33 | 0.46 | 2.46 | 1.72 | 2.70 | -2.03 | -0.53 | 2.93 | 2.09 | 1.89 | -2.17 |
Vacuole | -3.34 | -5.90 | -7.35 | -5.14 | -2.88 | 1.54 | 4.05 | -1.53 | -2.76 | -4.86 | -1.24 | -0.56 | -6.53 | -5.34 | -6.32 |
Vacuole Periphery | -4.12 | 1.41 | -4.06 | -1.43 | -4.59 | 1.27 | 6.17 | 2.33 | 0.56 | -5.23 | 0.15 | 6.42 | -0.37 | -0.51 | -7.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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