Standard name
Human Ortholog
Description Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0.05 0.06 0.05 0.06 0.07 0.11 0.14 0.08 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.09 0.22 0.46 0.63 0.56 0.66 0.72 0.69 0.75 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.96 0.94 0.92 0.87 0.82 0.74 0.72 0.71 0.66 0.71 0.62 0.9 0.81 0.74 0.85 0.87 0.82 0.93 0.95 0.93 0.73 0.53 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0 0 0 0.08 0 0 0 0 0 0.06 0.09 0 0 0.07 0 0 0 0 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.26 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0
Bud 0 0 0 1 1 0 9 8 10 9 7 9 0 0 0 0 1 0 1 1 0 1 5 3
Bud Neck 0 1 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 0 4 10 23 17 21 19 26 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 6 10 18 16 28 34 25 28 23 27 17 30 42 18 12 18 0 0 0 3 4 0
Endoplasmic Reticulum 0 0 1 0 1 1 0 0 0 0 0 1 1 1 0 6 2 4 0 0 0 2 1 3
Endosome 0 0 0 0 1 0 0 1 0 0 1 0 3 2 3 1 2 2 0 2 1 3 6 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 4 2
Mitochondria 4 5 43 29 89 213 384 453 328 362 308 364 0 1 2 3 3 2 0 1 1 2 6 8
Nucleus 273 314 341 283 360 383 454 587 355 335 315 303 231 221 215 193 223 195 263 309 338 172 149 136
Nuclear Periphery 2 2 1 6 18 7 10 14 4 8 12 3 2 2 4 5 7 6 0 0 1 0 2 5
Nucleolus 2 0 3 17 12 13 12 62 11 13 11 14 5 16 26 7 11 16 0 1 7 9 17 30
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 3 4 2 12 2 4 1 7 8 1 0 0 0 1 0 1 0 1
Vac/Vac Membrane 8 6 3 1 9 8 8 13 14 4 6 4 4 8 7 8 10 9 9 5 9 35 72 98
Unique Cell Count 284 327 362 309 413 468 611 811 500 506 445 487 258 272 290 226 256 238 282 327 364 238 279 303
Labelled Cell Count 291 332 400 347 513 652 931 1194 770 791 712 739 264 289 308 242 271 253 282 327 364 238 279 303


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.7 6.1 6.0 5.0 4.1 3.6 3.6 3.4 3.1 3.2 3.2 9.5 9.0 8.5 11.5 13.2 12.8 11.0 10.9 11.1
Std Deviation (1e-4) 1.7 2.0 1.2 1.4 1.2 0.8 1.1 1.0 0.9 0.8 0.8 1.0 2.4 2.4 2.2 3.8 4.8 4.8 2.5 2.6 2.3
Intensity Change (Log2) – – – -0.02 -0.3 -0.57 -0.77 -0.76 -0.85 -0.98 -0.92 -0.92 0.64 0.56 0.48 0.91 1.12 1.06 0.86 0.84 0.86


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 2.2 1.6 2.4 2.2 2.6 2.9 2.7 2.9 3.2 5.0 6.2 3.8 2.2 3.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 3.6 0 0 0 0 0 0 0 -5.7 -5.6 -5.6 -4.6 -5.0 -5.0
Nucleus -1.3 -3.3 -5.3 -7.8 -8.5 -8.5 -9.8 -8.5 -10.8 -2.1 -5.1 -7.2 -3.6 -3.1 -4.8
Nuclear Periphery 0 3.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.5 2.1 0 0 0 0 0 0 0 0 3.7 5.0 0 2.9 4.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.5 0 0 0 2.1 0 0 0 0 0 0 0 2.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.5202 44.7911 41.0558 34.9742 27.4332 36.2738 32.5503 49.0017 42.4401 46.6171 39.3824 43.3455 34.9474 49.877 42.0898 42.3013 42.2855 42.7011
Actin 0.025 0.0212 0.0096 0.009 0.0739 0.0205 0.0113 0.0023 0.0047 0.0001 0.003 0.0168 0.0039 0.0008 0.0117 0.0019 0.0005 0.0099
Bud 0.0007 0.0001 0.0004 0.0001 0.0004 0.0001 0.0002 0 0 0 0 0.0001 0 0 0 0 0 0
Bud Neck 0.0011 0.0002 0.0007 0.0002 0.0005 0.0001 0.0002 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001
Bud Periphery 0.0008 0.0002 0.0005 0.0001 0.0006 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001
Bud Site 0.0022 0.0004 0.0044 0.0006 0.0013 0.0001 0.0014 0.0001 0.0002 0 0.0002 0.0001 0.0003 0 0.0001 0 0 0.0001
Cell Periphery 0.0004 0.0008 0.0003 0.0003 0.0002 0.0001 0.0002 0 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.001 0 0.0009 0.0027 0.0008 0 0.0002 0 0 0 0.0002 0 0.0003 0 0.0005 0 0.0013 0
Cytoplasmic Foci 0.0151 0.0036 0.0061 0.0129 0.0138 0.0033 0.0209 0.0008 0.0069 0.0001 0.0134 0.0008 0.0086 0.0005 0.0022 0.0001 0.0031 0.0011
Eisosomes 0.0015 0.002 0.0034 0.0012 0.0009 0.0031 0.0007 0.0006 0.001 0.0001 0.0003 0.0009 0.0009 0.0003 0.0016 0.0004 0.0004 0.0009
Endoplasmic Reticulum 0.0003 0 0.0007 0.002 0.0021 0.0002 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0
Endosome 0.0043 0.0016 0.0063 0.0068 0.0327 0.0025 0.0035 0.0001 0.0001 0 0.0055 0.0006 0.0011 0.0004 0.0015 0.0001 0.0024 0.0004
Golgi 0.0064 0.0105 0.0054 0.005 0.0229 0.0289 0.0104 0.0006 0.0009 0.0001 0.0329 0.0018 0.0022 0.0038 0.0039 0.0009 0.0005 0.0054
Lipid Particles 0.0555 0.0492 0.0617 0.1024 0.0339 0.0543 0.0413 0.0032 0.0299 0.007 0.0941 0.0138 0.0559 0.0348 0.0369 0.0018 0.0361 0.0696
Mitochondria 0.8178 0.8495 0.8282 0.7968 0.797 0.8427 0.8396 0.9684 0.9283 0.9786 0.7576 0.9251 0.8697 0.9362 0.889 0.9777 0.9 0.8539
None 0.0045 0.0001 0.0081 0.0059 0.0015 0.0002 0.0003 0 0.0005 0 0.0002 0.0001 0.0007 0 0.0025 0 0.0017 0
Nuclear Periphery 0.0019 0.0003 0.0047 0.0085 0.0025 0.0005 0.0004 0 0 0 0.0006 0.0001 0.0013 0 0.0012 0 0.0064 0.0001
Nucleolus 0.0142 0.0065 0.0012 0.0061 0.0012 0.0024 0.002 0.0002 0.0011 0 0.0025 0.0009 0.0043 0.0003 0.0013 0 0.0018 0.0003
Nucleus 0.0025 0.0009 0.0011 0.0031 0.0006 0.0002 0.0003 0 0.0001 0 0.0002 0 0.0003 0 0.0019 0 0.0159 0
Peroxisomes 0.0323 0.0304 0.0418 0.0083 0.0066 0.0355 0.0375 0.0231 0.0225 0.0132 0.0649 0.0322 0.0418 0.011 0.0365 0.0168 0.0198 0.0487
Punctate Nuclear 0.0009 0 0.0028 0.0144 0.0017 0.0001 0.0012 0 0.0001 0 0.0079 0 0.0014 0 0.0022 0 0.008 0
Vacuole 0.0019 0.0058 0.002 0.0014 0.0014 0.0003 0.0018 0.0001 0.0002 0 0.0012 0.0003 0.0005 0.0005 0.0005 0 0.0003 0.0003
Vacuole Periphery 0.0097 0.0166 0.0097 0.0123 0.0035 0.0045 0.0262 0.0006 0.0033 0.0006 0.0148 0.0059 0.0065 0.0111 0.0059 0.0003 0.0016 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 120.8737 114.2282 103.0893 96.2612 94.0308 119.081 102.2296 102.1804 115.7475 95.0167
Translational Efficiency 1.6925 1.9552 1.9111 2.3364 2.3599 1.852 1.9624 1.9758 1.8277 1.9406

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
220 1778 241 1329 1750 1374 2729 2000 1970 3152 2970 3329

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2098.44 2420.57 3492.62 3746.25 1943.65 2162.69 3216.83 3975.48 1960.94 2308.16 3239.21 3883.97
Standard Deviation 665.77 744.06 1014.65 1143.35 616.09 693.78 1010.06 1168.83 623.74 733.80 1013.23 1164.15
Intensity Change Log 2 — 0.206030 0.734992 0.836130 — 0.154058 0.726871 1.032361 — 0.181273 0.731093 0.933823

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009292 0.028448 0.024054 0.024624 0.013609 0.023504 0.006131 0.009220 0.013127 0.026293 0.007585 0.015370
Bud Neck 0.010015 0.002520 0.017885 0.003249 0.003166 0.002676 0.001101 0.001243 0.003931 0.002588 0.002463 0.002043
Bud Site 0.008344 0.001923 0.007209 0.004948 0.002775 0.004138 0.001169 0.002920 0.003397 0.002889 0.001659 0.003729
Cell Periphery 0.000114 0.000096 0.000146 0.000234 0.000086 0.000141 0.000045 0.000202 0.000089 0.000115 0.000053 0.000215
Cytoplasm 0.002161 0.001259 0.001112 0.000519 0.001660 0.001426 0.000572 0.000639 0.001716 0.001332 0.000616 0.000591
Cytoplasmic Foci 0.170759 0.066796 0.176397 0.017314 0.082436 0.061500 0.017126 0.010335 0.092300 0.064488 0.030050 0.013121
Eisosomes 0.006946 0.017215 0.007922 0.005923 0.014382 0.020067 0.006914 0.003168 0.013551 0.018458 0.006996 0.004268
Endoplasmic Reticulum 0.000245 0.000126 0.000216 0.000307 0.000170 0.000199 0.000023 0.000134 0.000178 0.000158 0.000038 0.000203
Endosome 0.001512 0.000930 0.003212 0.001515 0.000827 0.001195 0.000236 0.000863 0.000903 0.001046 0.000478 0.001123
Golgi 0.013167 0.041538 0.030728 0.029252 0.021850 0.028469 0.004412 0.020318 0.020881 0.035842 0.006548 0.023885
Lipid Particles 0.020556 0.028668 0.044842 0.007258 0.015531 0.017687 0.002849 0.006037 0.016092 0.023881 0.006256 0.006525
Mitochondria 0.445283* 0.580473* 0.490198* 0.869257* 0.499589* 0.658042* 0.892306* 0.921079* 0.493524* 0.614287* 0.859677* 0.900391*
Mitotic Spindle 0.014154 0.002090 0.047798 0.002436 0.002171 0.001156 0.000624 0.001084 0.003510 0.001683 0.004452 0.001624
None 0.002756 0.002204 0.003546 0.004407 0.001919 0.003131 0.001499 0.003554 0.002013 0.002608 0.001665 0.003895
Nuclear Periphery 0.000953 0.000275 0.003982 0.000653 0.000189 0.000213 0.000091 0.000250 0.000274 0.000248 0.000406 0.000411
Nuclear Periphery Foci 0.001175 0.000159 0.001453 0.000263 0.000306 0.000224 0.000050 0.000087 0.000403 0.000187 0.000164 0.000158
Nucleolus 0.014566 0.001539 0.008705 0.001024 0.003538 0.001867 0.000981 0.000567 0.004770 0.001682 0.001608 0.000750
Nucleus 0.000650 0.000103 0.000420 0.000496 0.000262 0.000903 0.000070 0.000450 0.000305 0.000452 0.000099 0.000468
Peroxisomes 0.265986* 0.220539* 0.108448* 0.011219 0.331200* 0.168562* 0.062062 0.006819 0.323917* 0.197882* 0.065826 0.008576
Vacuole 0.006688 0.001804 0.014362 0.004458 0.002979 0.003582 0.001019 0.003601 0.003393 0.002579 0.002102 0.003943
Vacuole Periphery 0.004677 0.001293 0.007365 0.010644 0.001354 0.001319 0.000720 0.007430 0.001725 0.001304 0.001259 0.008713

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.67 -2.76 -4.71 1.18 -0.11 -4.50 6.03 3.08 6.65 -2.73 -7.65 4.59 -1.48 5.94 -5.69
Bud Neck 5.90 -3.09 4.86 -1.33 6.28 1.17 6.63 5.30 3.87 -0.62 3.86 3.90 4.67 1.55 1.07
Bud Site 3.58 0.51 1.57 -2.88 1.32 -1.43 3.36 -0.30 0.99 -2.39 0.77 3.59 -0.62 -1.25 -3.39
Cell Periphery 0.41 -1.27 -2.41 -2.39 -1.89 -2.18 4.19 -3.24 -1.48 -4.37 -1.12 3.90 -4.37 -2.80 -5.66
Cytoplasm 2.39 2.75 4.23 7.56 5.07 0.75 7.75 3.20 2.03 -0.75 2.43 8.26 5.46 3.59 -0.36
Cytoplasmic Foci 8.05 -0.36 11.96 16.06 13.74 5.09 20.91 23.24 16.62 4.41 7.81 18.21 24.95 24.24 9.05
Eisosomes -7.02 -0.71 0.84 10.13 2.07 -3.53 7.22 12.58 12.11 5.70 -4.41 6.97 11.44 16.58 4.44
Endoplasmic Reticulum 3.68 0.77 -1.10 -3.70 -1.75 -0.96 8.94 1.30 1.90 -5.41 1.01 9.22 -0.95 -1.74 -7.15
Endosome 1.80 -2.05 -0.06 -1.64 1.98 -1.72 7.50 -0.24 1.15 -3.35 -1.08 4.21 -1.22 -0.44 -3.44
Golgi -8.81 -3.95 -4.61 3.68 0.32 -2.79 12.87 0.70 3.07 -8.79 -7.81 11.19 -1.58 5.69 -11.19
Lipid Particles -2.00 -3.10 3.54 9.64 5.33 -1.12 10.51 6.40 6.48 -3.15 -4.62 7.60 7.31 11.83 -0.28
Mitochondria -5.27 -1.33 -16.83 -27.37* -16.35 -12.74 -42.03* -44.28* -26.40* -4.98 -11.90 -39.82* -45.96* -39.95* -7.01
Mitotic Spindle 3.74 -4.31 3.53 -0.48 6.32 3.39 5.63 1.94 -0.03 -1.22 3.91 -1.24 3.01 -0.03 3.66
None 2.15 -2.00 -5.02 -9.77 -2.33 -2.44 3.97 -8.33 -0.80 -11.58 -2.55 3.40 -11.66 -5.00 -15.56
Nuclear Periphery 3.80 -5.06 1.53 -4.62 5.76 -1.15 6.20 -2.51 -1.51 -7.36 0.97 -2.37 -3.29 -4.51 -0.09
Nuclear Periphery Foci 3.98 -0.75 3.40 -1.28 4.08 0.98 4.25 3.52 2.21 -3.30 3.23 3.65 3.48 0.60 0.06
Nucleolus 3.49 1.37 3.62 1.89 4.02 3.39 5.52 6.67 5.19 2.19 5.25 5.15 6.88 5.54 3.53
Nucleus 4.19 1.45 0.30 -1.67 -0.47 -1.52 4.52 -1.41 0.93 -2.59 -0.83 4.98 -1.44 -0.22 -2.86
Peroxisomes 2.29 7.46 13.47 31.93* 10.10 16.45 33.68* 42.82* 24.86 19.12* 15.04 34.47* 44.68* 40.56* 20.84*
Vacuole 4.32 -3.04 1.71 -3.86 4.14 -0.66 4.37 -0.86 -0.06 -3.82 1.46 2.77 -1.00 -2.30 -3.51
Vacuole Periphery 2.56 -1.58 -3.37 -7.68 -2.02 0.12 2.31 -7.43 -7.73 -8.59 1.38 1.55 -9.68 -10.86 -10.95
Standard name
Human Ortholog
Description Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
Localization
Cell Percentages mitochondrion (84%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp5

Atp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available