Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.98 0.99 0.98 0.96 0.94 0.95 0.87 0.87 0.75 0.78 0.99 0.99 0.99 0.94 0.93 – 0.81 0.77 0.74 0.69 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.07 0.25 0.26 0.37 0.27 0 0 0 0.06 0 – 0.06 0.1 0.12 0.16 0.16 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 – 3 2 3 8 10 8
Bud 0 2 0 0 1 0 1 1 4 4 8 1 1 0 0 0 0 – 2 3 2 2 9 18
Bud Neck 1 0 10 8 13 1 16 13 6 8 6 13 0 0 0 0 0 – 1 0 1 3 5 5
Bud Site 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0 – – – – – – –
Cell Periphery 0 2 4 5 1 0 1 0 1 2 0 0 0 0 0 3 0 – 0 1 4 5 7 6
Cytoplasm 247 239 299 270 285 245 424 382 279 275 140 181 381 478 472 105 115 – 209 201 208 252 269 285
Endoplasmic Reticulum 1 2 1 1 1 0 0 0 0 0 0 0 1 0 2 10 9 – 3 0 0 4 3 5
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 – 4 8 2 2 3 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 – 1 2 0 1 1 1
Mitochondria 4 10 0 1 0 11 31 27 80 81 68 63 3 2 1 7 3 – 16 25 33 58 67 59
Nucleus 0 0 2 1 2 0 2 8 2 4 0 6 0 1 2 0 2 – 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 4 2 1 1 4 2 7 2 6 7 0 1 1 0 1 – 3 2 7 2 9 6
Vac/Vac Membrane 0 3 1 0 0 4 6 2 0 3 0 1 0 1 0 4 7 – 5 5 4 14 16 13
Unique Cell Count 251 252 305 274 290 254 450 403 322 317 186 233 384 481 477 112 123 257 263 283 369 420 422
Labelled Cell Count 253 259 321 288 304 263 487 435 379 379 229 276 387 483 479 130 138 257 263 283 369 420 422


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.9 4.9 4.4 4.7 4.9 3.7 4.2 3.1 3.2 3.0 3.5 3.6 3.8 3.8 6.0 5.9 – 3.6 3.6 4.0
Std Deviation (1e-4) 0.9 1.0 2.6 2.4 1.6 5.4 1.4 2.0 1.4 1.4 1.5 1.2 1.1 1.3 0.9 1.9 2.1 – 1.4 1.6 2.0
Intensity Change (Log2) – – – -0.17 -0.08 -0.0 -0.43 -0.24 -0.65 -0.63 -0.73 -0.5 -0.46 -0.37 -0.38 0.28 0.26 – -0.45 -0.44 -0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8222 1.1172 0.7908 2.0054 0.4275 0.9733 -1.0975 -0.7453 -0.8875 -1.1008 -0.662 -0.8634 -1.3105 -0.918 -1.1054 -0.3705 -0.7248 -1.0977
Actin 0.0145 0.0001 0.0019 0.0332 0.0365 0.0067 0.0255 0.003 0.0102 0 0.0033 0.005 0.0247 0.0004 0.0109 0.0527 0.0117 0.0045
Bud 0.0007 0.0011 0.0004 0.0006 0.0007 0.0007 0.0012 0.0029 0.0051 0.0006 0.0007 0.0012 0.0093 0.0401 0.0353 0.0028 0.0156 0.0213
Bud Neck 0.0007 0.0001 0.0005 0.0112 0.0007 0.0004 0.0012 0.0002 0.001 0.0001 0.0002 0.0007 0.0005 0.0008 0.0011 0.0007 0.0006 0.0053
Bud Periphery 0.0014 0.0003 0.0013 0.0011 0.0011 0.0034 0.0027 0.0047 0.002 0.0008 0.0029 0.002 0.0059 0.0113 0.0151 0.0023 0.0121 0.0122
Bud Site 0.002 0.0052 0.0025 0.0297 0.0019 0.0001 0.0056 0.007 0.0097 0.0002 0.001 0.0008 0.0098 0.0476 0.0199 0.001 0.0024 0.0036
Cell Periphery 0.0004 0.0001 0.0005 0.0009 0.0001 0.0001 0.0004 0.0002 0.0003 0 0.0002 0.0001 0.0006 0.001 0.0006 0.0002 0.0003 0.0006
Cytoplasm 0.0229 0.0187 0.0151 0.0081 0.0124 0.0292 0.0347 0.0279 0.0301 0.0202 0.0149 0.059 0.0256 0.0285 0.0251 0.0304 0.0253 0.0883
Cytoplasmic Foci 0.0133 0.0153 0.0073 0.0276 0.0149 0.0079 0.0355 0.0165 0.0061 0.0044 0.011 0.0109 0.0355 0.008 0.0358 0.0087 0.0076 0.0263
Eisosomes 0.0016 0.0002 0.0009 0.0004 0.0004 0.0001 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0005 0.0002 0.0003 0.0005 0.0003 0.0002
Endoplasmic Reticulum 0.0023 0.0002 0.0007 0.0087 0.0002 0.0002 0.0047 0.0006 0.0009 0.0001 0.0009 0.0007 0.0015 0.0005 0.0024 0.0009 0.0017 0.0015
Endosome 0.0154 0.0001 0.0059 0.0212 0.0024 0.0036 0.016 0.0078 0.0054 0.0002 0.0792 0.0073 0.0036 0.0002 0.0015 0.0036 0.0118 0.0023
Golgi 0.0064 0 0.0019 0.0104 0.0098 0.0013 0.0059 0.001 0.0017 0 0.0162 0.0077 0.0046 0.0001 0.0011 0.0158 0.005 0.0008
Lipid Particles 0.0422 0.0003 0.0094 0.0454 0.0014 0.0081 0.0193 0.0038 0.001 0.0001 0.005 0.0102 0.008 0.0002 0.0026 0.0057 0.0161 0.0034
Mitochondria 0.0066 0.0002 0.0074 0.0076 0.0053 0.0013 0.017 0.0133 0.0016 0.0001 0.0237 0.003 0.0073 0.0025 0.0073 0.0385 0.0065 0.0038
None 0.809 0.9545 0.9308 0.6839 0.9013 0.926 0.773 0.8872 0.9056 0.9721 0.8192 0.8729 0.815 0.7967 0.7559 0.8079 0.8311 0.7459
Nuclear Periphery 0.0062 0.0001 0.0011 0.0026 0.0001 0.0006 0.0017 0.0006 0.0005 0.0001 0.0005 0.0014 0.0016 0.0015 0.0024 0.0021 0.0065 0.0045
Nucleolus 0.0029 0.001 0.0019 0.0216 0.0006 0.0001 0.0007 0.0007 0.0011 0.0001 0.0002 0.0002 0.0034 0.0042 0.0067 0.0013 0.0026 0.0034
Nucleus 0.0082 0.0006 0.0016 0.0029 0.0003 0.0002 0.001 0.0008 0.0023 0.0002 0.0003 0.0012 0.0026 0.0269 0.0254 0.0062 0.0267 0.0244
Peroxisomes 0.0176 0.0001 0.0026 0.0756 0.0082 0.0084 0.0473 0.0149 0.0094 0 0.0025 0.0136 0.0244 0.0002 0.0023 0.0041 0.0033 0.0023
Punctate Nuclear 0.0232 0.0018 0.0043 0.0017 0.0016 0.0013 0.0021 0.0034 0.003 0.0001 0.0002 0.0012 0.0135 0.0279 0.0462 0.0133 0.0087 0.0428
Vacuole 0.0016 0.0002 0.0016 0.0045 0.0002 0.0001 0.0033 0.002 0.0023 0.0004 0.0143 0.0006 0.0015 0.001 0.0015 0.0009 0.003 0.0019
Vacuole Periphery 0.0007 0 0.0004 0.0012 0.0001 0.0001 0.0009 0.0014 0.0006 0 0.0035 0.0004 0.0006 0.0002 0.0004 0.0005 0.0012 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.4924 22.6369 19.2759 27.4308 22.0674 21.5746 24.9519 26.844 26.6367 31.2955
Translational Efficiency 0.8605 0.8572 0.9953 0.6149 0.8869 0.8162 0.7792 0.6462 0.7161 0.6365

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1208 1229 516 1367 1922 1570 153 170 3130 2799 669 1537

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 619.63 712.18 877.75 788.69 665.51 713.70 852.61 795.90 647.80 713.03 872.00 789.49
Standard Deviation 63.49 87.53 111.13 105.05 70.19 85.69 81.04 104.01 71.27 86.51 105.54 104.96
Intensity Change Log 2 — 0.200835 0.502403 0.348051 — 0.100857 0.357426 0.258127 — 0.149927 0.429147 0.302184

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000281 0.000512 0.000924 0.000073 0.000999 0.000168 0.000512 0.000079 0.000684 0.000433 0.000878
Bud Neck 0.022151 0.028145 0.004691 0.010166 0.012134 0.056440 0.001514 0.002624 0.016000 0.044016 0.003964 0.009332
Bud Site 0.004329 0.008614 0.010426 0.050824 0.002056 0.024593 0.001018 0.017339 0.002933 0.017577 0.008274 0.047121
Cell Periphery 0.000185 0.000138 0.000167 0.000187 0.000151 0.000199 0.000172 0.000358 0.000164 0.000172 0.000168 0.000206
Cytoplasm 0.482937* 0.450097* 0.100709* 0.428760* 0.485796* 0.363416* 0.129722* 0.465589* 0.484693* 0.401476* 0.107344* 0.432833*
Cytoplasmic Foci 0.212629* 0.237454* 0.005593 0.017140 0.217500* 0.283682* 0.017315 0.038421 0.215620* 0.263384* 0.008274 0.019494
Eisosomes 0.000305 0.000143 0.000060 0.000050 0.000175 0.000201 0.000097 0.000093 0.000225 0.000175 0.000068 0.000055
Endoplasmic Reticulum 0.000405 0.000399 0.007975 0.002292 0.000943 0.000406 0.002949 0.001832 0.000736 0.000403 0.006825 0.002241
Endosome 0.012000 0.017989 0.001800 0.003627 0.006938 0.024643 0.003052 0.004518 0.008892 0.021721 0.002086 0.003725
Golgi 0.002036 0.003991 0.000093 0.002733 0.001414 0.008789 0.000074 0.000113 0.001654 0.006682 0.000089 0.002443
Lipid Particles 0.012183 0.006971 0.002103 0.001362 0.009475 0.006899 0.003103 0.002434 0.010520 0.006931 0.002332 0.001481
Mitochondria 0.007595 0.010379 0.000701 0.001635 0.004798 0.020185 0.000371 0.000340 0.005877 0.015880 0.000626 0.001492
Mitotic Spindle 0.000333 0.002743 0.004198 0.025110 0.001181 0.011845 0.000289 0.018671 0.000854 0.007849 0.003304 0.024398
None 0.004658 0.002763 0.003859 0.005280 0.003294 0.002836 0.005023 0.029744 0.003820 0.002804 0.004125 0.007986
Nuclear Periphery 0.000410 0.000318 0.002332 0.001760 0.000468 0.000291 0.001854 0.001297 0.000446 0.000303 0.002222 0.001709
Nuclear Periphery Foci 0.000183 0.000277 0.001011 0.000894 0.000218 0.000194 0.000694 0.001268 0.000205 0.000230 0.000938 0.000935
Nucleolus 0.001435 0.001508 0.001498 0.000529 0.000934 0.001543 0.001857 0.001165 0.001128 0.001528 0.001580 0.000599
Nucleus 0.181206* 0.153294* 0.816804* 0.349609* 0.216453* 0.097368 0.774208* 0.259621* 0.202850* 0.121924 0.807062* 0.339656*
Peroxisomes 0.005544 0.014447 0.000341 0.000627 0.005735 0.026254 0.000379 0.000840 0.005661 0.021069 0.000350 0.000650
Vacuole 0.048449 0.058205 0.034929 0.095473 0.029447 0.066194 0.055850 0.152965 0.036781 0.062686 0.039714 0.101832
Vacuole Periphery 0.000939 0.001844 0.000200 0.001017 0.000816 0.003023 0.000291 0.000255 0.000864 0.002506 0.000221 0.000932

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.27 -9.12 -4.06 -2.38 1.24 -1.75 -5.07 -1.79 0.55 -1.24 -2.02 -9.82 -4.49 0.20 0.42
Bud Neck -1.84 7.59 7.22 8.50 -0.81 -15.13 11.23 9.93 18.72 -1.58 -13.34 9.62 8.12 18.41 -1.53
Bud Site -3.39 -1.76 -6.59 -5.17 -3.61 -10.92 1.23 -2.04 2.88 -2.15 -11.44 -2.15 -7.51 -1.73 -4.39
Cell Periphery 3.53 -0.37 3.76 -0.12 3.43 -2.20 -1.13 -1.91 -0.94 -1.46 -0.58 -1.70 1.18 1.48 2.57
Cytoplasm 3.02 40.70 15.64 11.89 -24.65 13.60 25.24 8.06 2.51 -7.20 11.97 52.90 20.12 9.33 -25.30
Cytoplasmic Foci -2.85 36.79 35.51 35.33 -5.83 -8.59 37.44 32.19 36.71 -3.01 -8.30 55.71 54.08 55.65 -5.32
Eisosomes 4.93 6.85 7.59 10.08 3.83 -1.63 4.78 2.46 3.34 -0.92 3.23 9.60 11.48 12.51 3.88
Endoplasmic Reticulum -0.00 -17.37 -19.26 -19.71 11.77 1.38 -5.76 -5.29 -8.18 0.82 1.38 -16.18 -10.82 -21.21 11.36
Endosome -3.47 9.24 9.40 11.47 0.56 -12.33 4.95 3.60 14.65 -1.83 -11.81 10.99 11.09 19.70 0.30
Golgi -2.81 7.45 2.17 3.94 -1.83 -8.20 8.03 7.82 9.84 -1.97 -8.56 10.68 1.95 8.44 -1.86
Lipid Particles 4.32 8.99 10.47 7.68 3.55 3.04 8.16 8.97 7.41 0.79 5.12 12.31 15.16 12.60 4.21
Mitochondria -1.17 4.21 4.03 6.46 -1.95 -8.50 5.78 5.93 12.16 1.34 -7.29 6.61 6.20 13.27 -2.35
Mitotic Spindle -3.10 -2.28 -7.58 -6.52 -3.84 -5.61 -0.24 -1.72 0.53 -1.61 -6.16 -2.11 -7.54 -4.33 -4.47
None 1.97 1.39 -0.20 -4.31 -2.80 0.87 -0.49 -2.20 -2.30 -1.89 2.08 0.64 -2.73 -4.57 -3.00
Nuclear Periphery 1.12 -16.31 -17.24 -19.42 -0.90 2.63 -10.78 -9.12 -10.24 0.59 2.81 -19.21 -19.06 -20.62 -1.40
Nuclear Periphery Foci -3.64 -8.21 -11.14 -9.76 2.58 0.48 -5.39 -5.80 -5.85 -3.50 -1.50 -8.96 -12.33 -11.84 1.42
Nucleolus -0.23 -1.04 4.30 5.82 2.53 -3.88 -1.05 1.41 4.46 1.54 -3.00 -1.64 4.76 7.59 2.80
Nucleus 3.80 -52.30 -23.81 -26.71 29.83 21.14 -26.19 -3.22 -9.46 17.04 17.88 -58.47 -23.16 -33.72 33.21
Peroxisomes -5.67 6.49 6.70 10.79 0.64 -10.99 9.36 5.28 13.27 -0.96 -12.29 11.02 11.08 17.81 0.00
Vacuole -3.40 -3.03 -20.53 -18.67 -14.47 -12.43 -5.60 -12.59 -11.00 -8.92 -12.13 -7.07 -25.81 -21.19 -16.23
Vacuole Periphery -2.79 6.31 2.79 4.20 -1.42 -4.84 3.45 4.41 6.53 0.63 -5.57 6.18 2.83 6.85 -1.36
Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rsc3

Rsc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available