Standard name
Human Ortholog
Description Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0.09 0.08 0.14 0.14 0.11 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.07 0.07 0.08 0.07 0.06 0.08 0.06 0.09 0.06 0 0.12 0.12 0.17 0.34 0.32 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0.63 0.5 0.39 0.37 0.4 0.12 0.07 0 0 0 0 0 0.45 0.45 0.38 0.47 0.45 0.46 0.82 0.76 0.71 0.76 0.75 0.78
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.23 0 0.18 0.15 0.47 0.55 0.46 0.62 0.69 0.66 0.57 0 0 0 0.11 0.07 0 0 0 0 0 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0 0.1 0.22 0.21 0.26 0.19 0.18 0.16 0.15 0.16 0.26 0 0 0.11 0 0 0 0 0 0.06 0 0 0
SpindlePole 0.47 0.34 0.7 0.54 0.49 0.43 0.41 0.5 0.37 0.32 0.36 0.42 0.54 0.56 0.52 0.25 0.34 0.37 0.1 0.09 0.15 0.12 0.06 0
Vac/Vac Membrane 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 6 2 0 1 0 0 0 1 0 0 0 10 10 6 1 4 4 0 3 6 0 4 5
Bud 9 13 11 7 21 50 52 84 73 72 78 73 8 8 4 0 4 4 0 1 1 1 1 1
Bud Neck 0 1 16 4 1 0 6 9 4 3 5 1 18 18 9 0 2 0 1 4 1 1 2 2
Bud Site 0 0 3 0 0 5 6 13 13 23 28 24 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 20 39 21 25 38 36 47 32 60 40 25 54 54 65 49 66 48 5 2 2 2 10 4
Endoplasmic Reticulum 0 3 2 2 1 3 0 2 0 0 1 0 10 5 3 2 12 11 0 0 1 2 4 2
Endosome 307 185 211 108 134 72 48 26 13 6 6 27 209 206 143 68 92 79 455 355 431 275 444 425
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 3 3 2 5 6
Mitochondria 60 84 16 53 51 276 358 286 323 457 463 393 1 1 0 16 15 6 9 17 10 11 34 32
Nucleus 2 2 0 2 0 3 3 4 1 2 0 1 0 0 3 0 0 0 0 1 0 0 0 0
Nuclear Periphery 1 3 2 0 1 3 1 0 1 2 1 3 2 2 1 2 0 0 1 1 0 1 0 1
Nucleolus 25 21 8 5 4 16 19 16 12 18 16 22 4 4 5 0 0 1 2 4 3 1 3 1
Peroxisomes 80 10 54 66 68 153 124 111 83 99 113 175 2 3 41 2 1 1 11 14 36 12 16 18
SpindlePole 229 126 380 159 163 249 270 308 191 215 253 290 249 260 196 37 71 63 55 40 92 43 34 23
Vac/Vac Membrane 21 24 4 4 3 5 4 2 1 0 1 3 9 6 16 6 14 9 4 12 3 5 21 12
Unique Cell Count 487 369 540 294 331 582 652 622 518 666 702 685 460 461 377 146 206 171 555 471 603 363 591 544
Labelled Cell Count 757 498 750 431 473 873 928 908 748 957 1007 1037 576 577 492 183 282 226 555 471 603 363 591 544


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.9 4.4 4.0 3.8 3.1 3.0 3.3 3.0 2.7 2.8 2.9 5.8 5.8 5.8 5.9 6.3 6.6 5.7 5.9 6.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.2 0.8 1.1 1.0 0.9 0.9 0.8 0.6 0.9 0.9 1.3 1.7 1.8 2.0 1.3 1.4 1.5
Intensity Change (Log2) – – – -0.13 -0.2 -0.47 -0.53 -0.41 -0.52 -0.69 -0.66 -0.6 0.42 0.42 0.41 0.43 0.53 0.6 0.38 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.6 2.6 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -0.3 -0.3 -1.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0.8 0.8 -0.5 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.2 -0.5 -1.2 0.2 -0.7 1.1 -1.1 -2.8 2.5 2.4 4.7 8.4 8.7 7.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.6 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 2.0 1.8 -0.3 1.6 1.4 1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.3 -3.4 -3.4 4.1 2.6 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 -0.9 -0.9 -0.2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -6.6 -6.4 0.4 -3.4 -4.4 -4.0
SpindlePole 0 0 0 0 0 0 0 0 0 -5.3 -4.6 -5.7 -9.9 -9.0 -7.9
Vacuole 0 0 0 0 0 0 0 0 0 1.7 0 3.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0073 0.0006 0.0009 0.0008 0.0008 0.0025 0.001 0.0014 0.0009 0.0001 0.0019 0.0003
Bud – – – – – – 0.0002 0.0002 0.0005 0.0001 0 0.0001 0.0004 0.001 0.0033 0.0009 0.0002 0.0013
Bud Neck – – – – – – 0.0006 0.0007 0.0009 0.0003 0.0002 0.0007 0.0005 0.0005 0.0008 0.0003 0.0002 0.0007
Bud Periphery – – – – – – 0.0003 0.0003 0.0007 0.0001 0 0.0001 0.0005 0.0004 0.0018 0.0001 0.0001 0.0008
Bud Site – – – – – – 0.0007 0.0015 0.001 0.0002 0.0001 0.0002 0.0021 0.0046 0.0021 0.0006 0.0001 0.0014
Cell Periphery – – – – – – 0.0001 0.0003 0.0001 0 0 0.0001 0.0004 0.0001 0.0001 0.0002 0 0.0002
Cytoplasm – – – – – – 0.0051 0.0223 0.0048 0.002 0.0039 0.0129 0.0114 0.0123 0.0086 0.0039 0.0061 0.0114
Cytoplasmic Foci – – – – – – 0.2559 0.2791 0.2746 0.2139 0.2788 0.2934 0.2637 0.2882 0.2744 0.2919 0.3151 0.2847
Eisosomes – – – – – – 0.0002 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum – – – – – – 0.0008 0.0013 0.0003 0.0002 0.0001 0.001 0.0011 0.0006 0.0004 0.0001 0.0003 0.0005
Endosome – – – – – – 0.2739 0.257 0.2366 0.242 0.2379 0.1996 0.2518 0.3248 0.2369 0.2169 0.2043 0.2086
Golgi – – – – – – 0.0292 0.0299 0.034 0.0349 0.0306 0.0249 0.0247 0.0277 0.0311 0.0289 0.033 0.0232
Lipid Particles – – – – – – 0.2242 0.1327 0.1692 0.2268 0.1373 0.2359 0.2007 0.1293 0.1641 0.1785 0.1308 0.2048
Mitochondria – – – – – – 0.0059 0.011 0.005 0.0384 0.005 0.0032 0.0038 0.004 0.0065 0.0067 0.0129 0.0023
None – – – – – – 0.01 0.0573 0.0429 0.0313 0.0219 0.036 0.0429 0.03 0.0451 0.0441 0.0415 0.0427
Nuclear Periphery – – – – – – 0.0009 0.0006 0.0003 0.0003 0.0002 0.0016 0.0009 0.0007 0.0006 0.0001 0.0003 0.0006
Nucleolus – – – – – – 0.0004 0.0012 0.0005 0.0001 0.0002 0.0008 0.0009 0.0022 0.0013 0.006 0.0003 0.0005
Nucleus – – – – – – 0.0004 0.0005 0.0004 0.0001 0.0002 0.0008 0.0009 0.0016 0.0008 0.001 0.0002 0.0005
Peroxisomes – – – – – – 0.1754 0.1862 0.2168 0.2054 0.2575 0.1663 0.1795 0.1559 0.2076 0.2143 0.2454 0.2019
Punctate Nuclear – – – – – – 0.0038 0.0071 0.0041 0.0013 0.0236 0.0161 0.007 0.005 0.0084 0.002 0.0047 0.0093
Vacuole – – – – – – 0.0041 0.0089 0.0054 0.0012 0.0012 0.0033 0.0048 0.0081 0.004 0.0026 0.0016 0.0034
Vacuole Periphery – – – – – – 0.0008 0.0011 0.0007 0.0006 0.0004 0.0007 0.0009 0.0014 0.0009 0.0007 0.001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4151 6.3719 6.6196 4.5467 4.1588 6.4116 6.6037 7.3922 5.6745 6.6696
Translational Efficiency 0.5999 0.7213 0.5032 0.5418 0.8924 0.5702 0.9295 0.7344 0.9734 0.8069

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
133 1409 373 1216 2336 1173 470 1023 2469 2582 843 2239

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 915.45 756.14 923.36 815.11 665.06 743.44 873.20 814.15 678.55 750.37 895.39 814.67
Standard Deviation 146.42 111.15 96.41 122.80 82.95 99.92 104.35 109.57 104.21 106.39 103.94 116.94
Intensity Change Log 2 — -0.275828 0.012412 -0.167486 — 0.160732 0.392828 0.291810 — -0.075832 0.184847 0.043827

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004401 0.000289 0.001017 0.002685 0.000174 0.000163 0.000542 0.000632 0.000402 0.000232 0.000752 0.001747
Bud Neck 0.012740 0.005566 0.017426 0.010079 0.007864 0.008301 0.009848 0.014295 0.008127 0.006808 0.013201 0.012005
Bud Site 0.040089 0.002777 0.012207 0.026591 0.001252 0.001749 0.008487 0.018658 0.003344 0.002310 0.010133 0.022967
Cell Periphery 0.000127 0.000037 0.000061 0.000047 0.000057 0.000055 0.000034 0.000040 0.000061 0.000045 0.000046 0.000044
Cytoplasm 0.007677 0.006364 0.004906 0.011622 0.006809 0.007843 0.003954 0.017306 0.006856 0.007036 0.004375 0.014219
Cytoplasmic Foci 0.145410 0.191881* 0.148027 0.218007* 0.167100 0.146951 0.146239 0.166847 0.165931 0.171469 0.147030 0.194632*
Eisosomes 0.000051 0.000042 0.000058 0.000029 0.000063 0.000067 0.000039 0.000040 0.000063 0.000053 0.000047 0.000034
Endoplasmic Reticulum 0.000925 0.000176 0.001465 0.000425 0.000324 0.000198 0.000760 0.000245 0.000357 0.000186 0.001072 0.000343
Endosome 0.507483* 0.585964* 0.489819* 0.394088* 0.569324* 0.577225* 0.492086* 0.496104* 0.565993* 0.581994* 0.491083* 0.440699*
Golgi 0.031368 0.064559 0.015590 0.017239 0.077848 0.060695 0.006881 0.015158 0.075344 0.062804 0.010735 0.016288
Lipid Particles 0.005508 0.037707 0.018884 0.014430 0.066408 0.066210 0.021513 0.016077 0.063127 0.050656 0.020350 0.015182
Mitochondria 0.007741 0.008461 0.001289 0.003156 0.015278 0.021492 0.000636 0.003353 0.014872 0.014381 0.000925 0.003246
Mitotic Spindle 0.046638 0.011111 0.042924 0.083073 0.002278 0.002667 0.107646* 0.056788 0.004668 0.007275 0.079008 0.071063
None 0.000033 0.000282 0.000131 0.000098 0.000784 0.000333 0.000079 0.000473 0.000743 0.000305 0.000102 0.000269
Nuclear Periphery 0.000251 0.000133 0.001262 0.000404 0.000139 0.000211 0.001296 0.000330 0.000145 0.000169 0.001281 0.000370
Nuclear Periphery Foci 0.002822 0.001892 0.024194 0.008774 0.000666 0.001076 0.035923 0.007181 0.000782 0.001521 0.030733 0.008046
Nucleolus 0.001098 0.000929 0.010170 0.001414 0.000929 0.002520 0.005929 0.004395 0.000938 0.001651 0.007805 0.002776
Nucleus 0.000926 0.001791 0.016662 0.002469 0.003000 0.004537 0.004484 0.006474 0.002889 0.003038 0.009872 0.004299
Peroxisomes 0.104101* 0.068689 0.119221* 0.156586* 0.070438 0.081964 0.109805* 0.124466* 0.072252 0.074720 0.113972* 0.141910*
Vacuole 0.072014 0.009652 0.072249 0.047317 0.007980 0.013177 0.043108 0.048484 0.011430 0.011254 0.056002 0.047851
Vacuole Periphery 0.008598 0.001697 0.002436 0.001466 0.001285 0.002567 0.000711 0.002653 0.001679 0.002092 0.001474 0.002009

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.40 1.11 0.49 -4.39 -2.46 0.23 -3.45 -3.69 -3.86 0.14 0.98 -1.75 -4.06 -4.99 -2.61
Bud Neck 2.43 -1.41 0.03 -5.15 2.15 -0.38 -2.90 -4.27 -3.70 -0.94 1.17 -4.59 -4.82 -6.09 0.74
Bud Site 3.32 2.39 1.05 -9.65 -5.01 -1.41 -7.41 -7.92 -7.63 -3.00 1.39 -7.11 -11.16 -12.27 -5.94
Cell Periphery 3.15 1.99 2.63 -2.10 1.92 0.11 0.68 0.87 1.62 0.48 0.96 0.32 0.85 -0.36 1.53
Cytoplasm 0.81 0.67 -3.73 -5.42 -5.01 -0.75 -0.58 -5.61 -5.01 -4.55 -0.25 -0.38 -7.37 -7.34 -6.49
Cytoplasmic Foci -4.21 -0.15 -5.58 -2.57 -7.62 3.54 3.95 0.80 -2.33 -2.99 -1.44 4.29 -4.78 -3.40 -8.08
Eisosomes 1.18 -0.74 1.85 2.26 5.30 -0.65 1.37 2.51 3.40 1.24 1.49 0.60 4.05 4.06 4.50
Endoplasmic Reticulum 2.88 -1.45 1.32 -3.59 2.92 0.90 -3.96 -0.31 -2.99 4.40 1.36 -4.84 -1.02 -4.40 4.72
Endosome -3.76 0.25 4.28 19.47 6.74 -0.47 7.87 9.49 8.55 -0.70 -1.75 8.95 17.91 19.77 4.46
Golgi -5.34 2.09 2.07 17.40 -0.16 4.61 28.40 23.37 15.07 -5.52 4.13 26.17 24.25 23.01 -3.91
Lipid Particles -9.80 -3.74 -4.36 7.32 1.12 -0.18 10.57 13.91 10.58 2.16 2.95 11.57 14.71 12.78 2.42
Mitochondria -0.38 1.44 0.99 4.32 -2.05 -2.45 12.22 9.29 8.00 -4.30 0.29 12.06 9.45 9.01 -4.03
Mitotic Spindle 3.29 0.83 -1.98 -13.25 -5.09 -0.78 -10.81 -12.34 -12.22 3.98 -1.56 -11.92 -18.43 -17.83 0.31
None -2.42 -2.07 -2.55 1.21 -0.00 1.46 2.22 0.70 -1.13 -2.54 1.51 2.16 1.42 -0.24 -2.32
Nuclear Periphery 3.17 -2.99 -2.54 -6.37 2.30 -1.36 -5.03 -6.74 -5.40 3.50 -0.48 -6.11 -8.04 -8.35 4.11
Nuclear Periphery Foci 1.72 -4.42 -2.32 -5.82 3.39 -1.82 -7.83 -5.30 -4.81 6.04 -2.49 -9.65 -8.37 -7.55 6.82
Nucleolus 1.34 -3.71 0.01 -2.86 3.88 -2.11 -4.59 -4.32 -2.97 1.44 -1.19 -6.17 -4.48 -3.70 3.99
Nucleus -0.79 -3.80 -2.76 -2.03 3.08 -1.19 -3.01 -2.78 -1.63 0.47 -0.21 -4.36 -2.65 -2.55 3.03
Peroxisomes 2.03 -0.97 -2.85 -11.23 -2.86 -2.26 -3.91 -7.49 -4.89 -1.50 -0.75 -5.81 -12.14 -11.66 -3.39
Vacuole 5.27 0.64 1.03 -13.90 0.60 -3.16 -9.23 -12.34 -11.03 -1.20 0.60 -11.70 -16.95 -17.65 -0.51
Vacuole Periphery 1.77 1.56 1.86 0.97 2.49 -2.41 2.53 -1.52 0.16 -2.22 -1.26 0.43 -0.51 0.53 -0.89
Standard name
Human Ortholog
Description Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
Localization
Cell Percentages endosome (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pep7

Pep7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pep7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available