Standard name
Human Ortholog
Description ER membrane glycoprotein subunit of the GPI transamidase complex; adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.07 – 0.16 0.16 0 0 0 0 0 0
Cytoplasm 0 0.14 0.05 0.07 0.1 0.11 0.11 0.09 0.13 0.14 0.19 0.17 0.09 0.09 0.11 – 0.41 0.45 0 0 0 0 0 0
Endoplasmic Reticulum 0.86 0.76 0.85 0.61 0.53 0.38 0.4 0.42 0.27 0.34 0.29 0.27 0.85 0.85 0.83 – 0 0 0.86 0.8 0.89 0.86 0.87 0.84
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Mitochondria 0.06 0.1 0.08 0.12 0.1 0.31 0.24 0.29 0.45 0.45 0.51 0.49 0 0 0 – 0.15 0.19 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0.1 0.07 0.19 0.15 0.08 0.09 0.1 0.1 0.06 0.05 0 0 0 0 0 – 0 0.06 0.07 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.05 0.1 0.24 0.33 0.33 0.34 0.29 0.28 0.21 0.16 0.19 0.08 0.08 0.07 – 0.28 0.11 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 14 11 3 8 11 26 6 15 12 19 10 11 15 – 7 6 2 0 13 4 25 19
Bud 1 1 0 0 0 6 5 6 8 7 24 15 1 1 2 – 0 2 0 0 1 2 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 1 1 3 4
Bud Site 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 2 15 7 10 20 30 31 24 28 22 48 29 29 43 – 15 10 0 0 3 3 19 29
Cytoplasm 2 8 25 21 43 59 86 75 71 99 106 125 48 48 66 – 39 29 0 2 1 7 11 13
Endoplasmic Reticulum 60 45 465 189 229 206 313 346 146 243 162 195 441 433 518 – 2 1 87 67 526 355 695 616
Endosome 0 0 3 6 9 12 14 19 7 4 6 6 1 0 0 – 1 0 0 1 3 1 2 3
Golgi 0 0 5 11 5 3 3 4 0 0 0 0 1 1 1 – 1 3 0 0 1 1 5 3
Mitochondria 4 6 44 38 43 169 190 240 243 319 291 357 0 0 0 – 14 12 2 3 8 11 19 15
Nucleus 0 0 2 0 2 5 7 5 9 5 4 5 5 5 5 – 3 0 0 0 0 0 0 0
Nuclear Periphery 7 4 104 46 34 50 77 80 33 39 24 31 8 6 6 – 0 4 6 2 24 9 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 2 1 1 0 0 1 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 – 1 0 0 0 0 0 0 0
Vac/Vac Membrane 9 3 57 73 144 181 265 236 153 153 90 141 41 42 42 – 27 7 2 4 4 12 13 24
Unique Cell Count 70 59 546 310 431 542 787 826 537 712 567 724 518 511 627 96 64 102 84 594 415 800 738
Labelled Cell Count 85 70 735 402 522 720 1001 1068 702 916 743 945 585 577 700 110 74 102 84 594 415 800 738


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.8 9.5 9.9 9.2 7.9 7.6 7.6 7.1 6.8 6.9 6.7 10.1 10.1 10.6 – 7.8 9.1 11.1 11.2 11.0
Std Deviation (1e-4) 1.7 1.5 1.9 2.1 1.8 1.6 1.7 1.6 1.4 1.5 1.5 1.4 1.4 1.4 1.5 – 1.5 1.8 1.7 1.8 1.8
Intensity Change (Log2) – – – 0.06 -0.05 -0.27 -0.32 -0.32 -0.42 -0.48 -0.46 -0.49 0.1 0.1 0.16 – -0.29 -0.07 0.22 0.24 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 -2.2 -1.3 -1.5 0.6 -1.8 -0.5 -0.5 0.1 -0.7 -0.4 -0.2 – 0 0
Bud 0 0 0 0 0 0 0 4.9 3.4 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery -0.4 -0.4 0.9 1.1 1.0 1.5 1.1 1.1 3.2 2.3 2.4 3.2 – 0 0
Cytoplasm 1.4 3.3 3.9 4.1 3.1 5.0 5.5 7.3 6.9 3.0 3.1 3.8 – 10.9 10.9
Endoplasmic Reticulum -8.0 -11.0 -16.0 -16.5 -16.0 -19.2 -18.1 -19.0 -20.6 0 -0.2 -1.2 – -16.9 -14.9
Endosome 0 2.2 2.4 2.0 2.5 0 0 0 0 0 0 0 – 0 0
Golgi 2.7 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 2.0 1.0 9.6 7.6 9.4 13.9 14.3 15.7 15.7 -6.6 -6.6 -7.2 – 2.1 2.8
Nucleus 0 0 0 0 0 2.1 0 0 0 0 0 0 – 0 0
Nuclear Periphery -1.6 -5.0 -4.6 -4.9 -5.0 -6.4 -7.5 -7.7 -8.5 -9.3 -9.5 -10.6 – -4.7 -2.5
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 5.1 8.8 9.2 9.7 8.0 7.5 5.2 2.7 4.4 -1.4 -1.2 -2.3 – 4.7 0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0251 0.0004 0.0145 0.0155 0.0246 0.0049 0.0441 0.0002 0.001 0.01 0.0002 0.0003 – – – – – –
Bud 0.0002 0.0001 0.0001 0.0008 0.0008 0.0001 0.0002 0 0.0001 0.0016 0 0 – – – – – –
Bud Neck 0.0015 0.0001 0.0036 0.0007 0.0438 0.0011 0.002 0.0001 0.0001 0.0288 0.0001 0.0008 – – – – – –
Bud Periphery 0.0004 0.0002 0.0004 0.0006 0.0023 0.0002 0.0003 0.0001 0.0002 0.0027 0 0 – – – – – –
Bud Site 0.0008 0.0012 0.0007 0.0008 0.0011 0.0003 0.0055 0.0011 0.0002 0.0037 0 0.0001 – – – – – –
Cell Periphery 0.0009 0.0011 0.0035 0.0002 0.0019 0.0003 0.0007 0.0009 0.0009 0.0008 0.0001 0.0001 – – – – – –
Cytoplasm 0.0498 0.0852 0.0746 0.0545 0.0441 0.0588 0.0549 0.0832 0.0351 0.0572 0.0028 0.0318 – – – – – –
Cytoplasmic Foci 0.0076 0.0024 0.0033 0.0248 0.0097 0.0045 0.0039 0.0021 0.0113 0.0097 0.0003 0.0075 – – – – – –
Eisosomes 0.0003 0.0001 0.0002 0.0002 0.0002 0 0.0002 0 0 0.0002 0 0 – – – – – –
Endoplasmic Reticulum 0.7873 0.8339 0.8422 0.6817 0.7358 0.851 0.8351 0.8602 0.8684 0.702 0.9656 0.8796 – – – – – –
Endosome 0.0209 0.0059 0.009 0.0281 0.01 0.0129 0.0107 0.0071 0.0191 0.0778 0.0115 0.0114 – – – – – –
Golgi 0.0049 0.0018 0.0026 0.0128 0.0023 0.0021 0.0038 0.0003 0.0067 0.0191 0.0008 0.0013 – – – – – –
Lipid Particles 0.008 0.0053 0.006 0.0299 0.0042 0.0053 0.0064 0.0028 0.0097 0.0085 0.0005 0.0089 – – – – – –
Mitochondria 0.0018 0.0142 0.0013 0.0043 0.0043 0.0006 0.0007 0.0001 0.0034 0.0411 0.0004 0.0004 – – – – – –
None 0.024 0.0275 0.0091 0.053 0.0176 0.004 0.0055 0.0097 0.0216 0.0078 0.0001 0.0207 – – – – – –
Nuclear Periphery 0.0327 0.0121 0.0201 0.0205 0.0623 0.0396 0.0182 0.0194 0.0113 0.0112 0.012 0.0305 – – – – – –
Nucleolus 0.0022 0.0003 0.0007 0.0021 0.0008 0.0004 0.0001 0.0001 0.0001 0.0004 0 0.0001 – – – – – –
Nucleus 0.0052 0.0025 0.0024 0.0102 0.0129 0.0047 0.001 0.0024 0.0006 0.002 0.0004 0.0014 – – – – – –
Peroxisomes 0.0082 0.0001 0.0004 0.0045 0.0008 0.0011 0.001 0 0.0027 0.0016 0 0.001 – – – – – –
Punctate Nuclear 0.0117 0.002 0.0019 0.0509 0.012 0.004 0.0029 0.0004 0.0003 0.0016 0 0.0017 – – – – – –
Vacuole 0.0048 0.0031 0.0025 0.0025 0.0055 0.0029 0.0025 0.009 0.0056 0.0081 0.0042 0.0017 – – – – – –
Vacuole Periphery 0.0017 0.0004 0.0008 0.0013 0.003 0.0011 0.0003 0.0008 0.0015 0.004 0.001 0.0006 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.7779 31.1235 17.1732 32.5224 43.4045 26.7668 31.8724 27.23 26.5799 41.2441
Translational Efficiency 0.9751 1.0074 1.4788 0.8841 0.7182 1.6142 1.0126 0.9511 0.9757 0.8374

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 1272 1584 872 1334 347 700 567 1437 1619 2284 1439

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1102.59 1085.04 1283.00 1096.34 853.77 976.53 1102.36 1100.69 871.60 1061.78 1227.64 1098.05
Standard Deviation 155.93 195.56 184.87 211.20 131.32 157.12 285.56 215.05 147.89 193.18 235.86 212.74
Intensity Change Log 2 — -0.023148 0.218625 -0.008201 — 0.193817 0.368676 0.366489 — 0.075572 0.286035 0.167383

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007309 0.000720 0.000448 0.002038 0.001379 0.000921 0.001013 0.001315 0.001804 0.000763 0.000622 0.001753
Bud Neck 0.003043 0.000822 0.000669 0.007780 0.000393 0.001285 0.001611 0.003975 0.000583 0.000921 0.000958 0.006281
Bud Site 0.028685 0.004950 0.001337 0.016056 0.002777 0.005170 0.006641 0.014924 0.004634 0.004997 0.002963 0.015610
Cell Periphery 0.006619 0.001640 0.000383 0.001570 0.003024 0.001623 0.000778 0.000994 0.003281 0.001637 0.000504 0.001343
Cytoplasm 0.083442 0.040063 0.010213 0.087843 0.022587 0.084537 0.073343 0.078633 0.026949 0.049595 0.029561 0.084214
Cytoplasmic Foci 0.032532 0.008884 0.000551 0.012411 0.006518 0.039299 0.011445 0.011630 0.008383 0.015403 0.003890 0.012103
Eisosomes 0.000666 0.000115 0.000012 0.000192 0.000265 0.000407 0.000018 0.000102 0.000294 0.000178 0.000014 0.000157
Endoplasmic Reticulum 0.535197* 0.877501* 0.869968* 0.592066* 0.921119* 0.726625* 0.721009* 0.685278* 0.893457* 0.845164* 0.824315* 0.628794*
Endosome 0.015623 0.005698 0.007554 0.019316 0.003597 0.012165 0.018656 0.017144 0.004459 0.007084 0.010957 0.018460
Golgi 0.005684 0.003433 0.000184 0.008002 0.004886 0.013281 0.004506 0.001398 0.004944 0.005544 0.001509 0.005400
Lipid Particles 0.025479 0.007221 0.001367 0.016179 0.006443 0.023248 0.006309 0.014176 0.007807 0.010656 0.002882 0.015389
Mitochondria 0.009317 0.002222 0.000364 0.004893 0.004983 0.004384 0.000990 0.001518 0.005294 0.002685 0.000556 0.003563
Mitotic Spindle 0.018894 0.000147 0.000809 0.017748 0.000318 0.000885 0.001316 0.001602 0.001650 0.000305 0.000964 0.011386
None 0.000186 0.002451 0.000481 0.001190 0.002163 0.001329 0.003384 0.000485 0.002021 0.002210 0.001371 0.000912
Nuclear Periphery 0.035466 0.009127 0.045253 0.035472 0.002003 0.019401 0.019894 0.016012 0.004402 0.011329 0.037481 0.027804
Nuclear Periphery Foci 0.050170 0.021949 0.051307 0.080073 0.008981 0.034950 0.089802 0.073362 0.011933 0.024736 0.063105 0.077428
Nucleolus 0.000092 0.000059 0.000120 0.000991 0.000126 0.000148 0.001212 0.000309 0.000124 0.000078 0.000455 0.000722
Nucleus 0.026090 0.004416 0.003211 0.022395 0.001233 0.010787 0.016060 0.017315 0.003015 0.005781 0.007149 0.020393
Peroxisomes 0.001191 0.000855 0.000029 0.000615 0.003650 0.002452 0.000334 0.000370 0.003474 0.001197 0.000123 0.000519
Vacuole 0.111532 0.006622 0.005345 0.067882 0.002512 0.015608 0.019810 0.055666 0.010326 0.008548 0.009778 0.063069
Vacuole Periphery 0.002783 0.001106 0.000394 0.005289 0.001042 0.001497 0.001869 0.003793 0.001167 0.001190 0.000846 0.004700

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.87 1.94 1.33 -2.82 -3.06 0.87 0.78 -0.29 -1.12 -1.06 2.21 2.40 -0.62 -3.12 -3.28
Bud Neck 2.58 2.84 -3.84 -6.16 -6.38 -2.26 -5.23 -5.53 -3.93 -3.69 -1.13 -2.37 -7.88 -7.22 -7.37
Bud Site 2.42 2.80 1.01 -5.73 -8.00 -1.37 -3.69 -4.27 -3.20 -2.94 -0.29 1.55 -6.64 -6.32 -7.94
Cell Periphery 0.89 1.13 0.91 0.16 -5.22 1.27 2.77 2.45 0.78 -1.07 1.80 3.29 2.26 0.76 -5.26
Cytoplasm 2.94 4.73 -0.86 -8.81 -13.88 -6.37 -8.35 -8.51 -0.46 -1.58 -5.73 -1.05 -13.08 -8.25 -12.53
Cytoplasmic Foci 3.45 4.71 2.79 -2.55 -10.28 -5.32 -2.72 -3.53 4.04 -0.64 -3.50 3.60 -3.56 0.89 -8.05
Eisosomes 1.78 2.13 1.51 -1.45 -3.79 -0.91 4.67 2.69 1.99 -4.28 1.76 5.19 2.14 0.33 -4.86
Endoplasmic Reticulum -9.15 -8.96 -0.61 22.34 22.37 9.60 14.51 16.02 3.23 3.44 5.13 8.36 25.54 20.95 19.99
Endosome 3.11 2.47 -1.06 -9.51 -7.97 -3.50 -11.25 -8.70 -1.68 0.91 -3.65 -10.16 -13.08 -9.35 -6.19
Golgi 0.60 1.61 -0.47 -1.98 -3.88 -2.52 0.31 4.91 3.65 3.22 -0.58 4.77 0.05 0.50 -2.95
Lipid Particles 2.96 3.98 1.32 -4.29 -7.72 -4.99 -0.04 -4.06 2.27 -3.94 -2.22 5.90 -5.11 -2.94 -8.96
Mitochondria 0.84 1.08 0.53 -1.43 -2.72 0.35 4.98 3.77 1.74 -0.94 2.36 5.09 1.42 -0.62 -2.91
Mitotic Spindle 1.62 1.55 -0.09 -5.62 -5.34 -1.47 -2.53 -2.09 -1.61 -1.22 1.56 0.61 -4.93 -5.89 -5.46
None -3.46 -1.38 -3.08 1.64 -2.00 1.05 -1.39 3.81 1.11 3.54 -0.29 1.38 2.43 2.16 1.13
Nuclear Periphery 2.79 -1.25 0.05 -6.43 2.62 -4.14 -8.06 -5.79 0.81 1.35 -4.63 -14.69 -8.98 -5.55 3.54
Nuclear Periphery Foci 2.82 -0.36 -2.71 -9.99 -4.25 -4.67 -12.33 -10.01 -4.58 1.99 -5.21 -16.27 -15.31 -11.37 -2.32
Nucleolus 1.36 -0.45 -1.86 -1.93 -1.77 -0.25 -2.36 -1.49 -1.47 1.77 0.61 -2.06 -2.04 -2.25 -0.82
Nucleus 2.96 3.07 -0.52 -9.48 -9.75 -4.99 -6.39 -7.69 -3.36 -1.43 -2.82 -4.05 -11.77 -10.45 -9.29
Peroxisomes 0.93 3.93 1.47 0.69 -3.63 1.48 5.47 5.56 3.73 0.01 3.98 6.17 5.29 2.88 -3.49
Vacuole 5.45 5.51 1.52 -14.25 -14.67 -4.19 -8.88 -10.66 -7.99 -7.82 1.26 0.74 -15.23 -16.72 -16.74
Vacuole Periphery 1.07 1.60 -1.25 -3.57 -4.51 -0.92 -2.55 -1.97 -1.55 -1.31 -0.10 1.29 -3.93 -3.78 -4.45
Standard name
Human Ortholog
Description ER membrane glycoprotein subunit of the GPI transamidase complex; adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gpi8

Gpi8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpi8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available