Standard name
Human Ortholog
Description Double-stranded DNA-dependent helicase of the DExH/D-box family; required for maintenance of the mitochondrial (mt) genome; null mutant accumulates double-stranded breaks in mt DNA; localizes to the mt matrix
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.49 0.55 0.83 0.75 0.75 0.56 0.57 – 0.41 0.34 0.28 0.36 0.96 0.92 0.95 0.55 0.5 0.54 0.1 0.09 0.12 0.27 0.3 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Endosome 0.07 0.11 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.13 0.1 0.1 0.11 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.21 0 0.12 0.16 0.39 0.34 – 0.55 0.55 0.77 0.62 0 0 0 0.11 0.06 0.06 0.61 0.57 0.6 0.5 0.49 0.54
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0 0.05 0.07 0.06 0.11 – 0.13 0.11 0.06 0.12 0 0 0 0.13 0.14 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.1 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.14 0.12 0.05 0 0.05 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 3 1 0 0 – 0 0 1 0 1 4 0 1 1 2 3 15 8 2 7 16
Bud 3 3 0 3 3 3 6 – 8 6 10 7 2 0 0 2 4 4 3 2 0 0 2 5
Bud Neck 2 3 8 6 4 3 6 – 3 5 1 4 3 3 0 3 9 3 1 5 2 2 6 7
Bud Site 0 1 0 0 0 0 0 – 1 0 7 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 2 2 1 2 1 – 0 2 0 1 5 6 4 7 2 1 0 2 3 2 2 6
Cytoplasm 93 147 135 109 149 129 134 – 82 76 65 75 230 347 360 91 98 45 24 38 25 64 107 105
Endoplasmic Reticulum 4 8 0 2 0 1 1 – 3 4 9 0 1 3 1 10 12 4 3 7 5 3 3 6
Endosome 13 29 6 4 7 7 6 – 5 5 0 3 2 11 3 16 25 8 24 45 13 7 13 15
Golgi 1 0 1 2 0 0 1 – 0 0 0 0 1 0 1 2 1 0 3 4 3 0 0 0
Mitochondria 83 57 1 17 31 91 80 – 110 123 181 128 2 2 1 18 12 5 154 234 131 118 175 244
Nucleus 0 1 2 2 0 3 5 – 3 4 3 4 1 0 0 2 3 4 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 2 2 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 0 2 0 0 1 0 1
Peroxisomes 6 6 1 1 5 3 3 – 0 2 0 1 2 5 18 4 6 0 3 4 0 0 0 2
SpindlePole 3 13 7 8 13 15 26 – 26 25 15 25 1 10 6 21 27 12 6 17 3 6 8 5
Vac/Vac Membrane 11 27 9 0 4 5 9 – 3 7 3 3 2 4 3 18 28 10 13 17 10 13 16 19
Unique Cell Count 191 268 163 146 198 232 234 199 224 235 206 240 377 380 166 197 83 254 410 218 239 357 451
Labelled Cell Count 220 303 173 159 218 262 279 244 259 296 253 253 395 397 196 228 98 254 410 218 239 357 451


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.6 5.3 5.0 5.2 4.2 4.7 – 3.7 4.1 3.4 3.6 5.3 5.3 5.0 5.7 6.2 5.6 4.8 4.9 5.1
Std Deviation (1e-4) 0.7 0.9 1.1 1.7 1.9 1.6 2.1 – 1.3 1.5 1.2 1.1 1.2 1.4 1.2 1.4 2.1 1.4 1.0 1.2 1.3
Intensity Change (Log2) – – – -0.08 -0.02 -0.34 -0.17 – -0.53 -0.38 -0.65 -0.58 -0.01 -0.01 -0.07 0.1 0.22 0.07 -0.14 -0.12 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.8 2.7 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2459 -1.1324 -1.0923 -1.4909 -1.1602 -1.3691 0.0081 0.0041 0.3912 0.32 0.728 0.0454 -0.4122 -0.2262 -0.4139 0.1053 -0.582 -0.288
Actin 0.0212 0.0189 0.032 0.0015 0.0176 0.0059 0.0242 0.0136 0.0405 0.0262 0.0807 0.0134 0.0053 0.0055 0.0074 0.0087 0.0018 0.0022
Bud 0.0007 0.0056 0.0016 0.0006 0.0027 0.0005 0.0027 0.0044 0.0139 0.0031 0.0041 0.004 0.0047 0.0116 0.0062 0.0016 0.0058 0.001
Bud Neck 0.009 0.0023 0.0023 0.0012 0.0033 0.0114 0.0068 0.0023 0.0032 0.0067 0.0167 0.0098 0.0023 0.0013 0.0019 0.0008 0.0028 0.0103
Bud Periphery 0.0023 0.014 0.0067 0.0014 0.0087 0.0016 0.0083 0.0077 0.0147 0.0092 0.016 0.0061 0.0045 0.0125 0.0102 0.004 0.0193 0.0025
Bud Site 0.0065 0.0188 0.0107 0.0005 0.0093 0.0007 0.0112 0.0268 0.0148 0.0078 0.0172 0.0038 0.0058 0.0182 0.0117 0.0011 0.0081 0.0014
Cell Periphery 0.0014 0.002 0.0016 0.0002 0.0056 0.0008 0.0013 0.0016 0.0011 0.0032 0.003 0.0009 0.0014 0.001 0.0012 0.0002 0.0005 0.0003
Cytoplasm 0.0666 0.0856 0.0845 0.0489 0.1384 0.1236 0.0678 0.1107 0.0598 0.0441 0.0467 0.0821 0.1292 0.1735 0.1277 0.0998 0.1536 0.1689
Cytoplasmic Foci 0.1234 0.178 0.1296 0.1309 0.1332 0.1606 0.1185 0.0959 0.0907 0.1272 0.1095 0.1104 0.16 0.1512 0.1335 0.1334 0.1458 0.1521
Eisosomes 0.0025 0.0016 0.0019 0.0003 0.0019 0.0005 0.0017 0.0016 0.0012 0.006 0.001 0.0004 0.0008 0.0005 0.0005 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0093 0.0083 0.0041 0.0032 0.0103 0.0024 0.0056 0.0038 0.0041 0.0028 0.0061 0.0019 0.0048 0.0021 0.0035 0.003 0.0021 0.0022
Endosome 0.0908 0.099 0.0681 0.089 0.1456 0.0704 0.0543 0.0496 0.0482 0.0342 0.0817 0.0422 0.0829 0.0773 0.0772 0.0974 0.0835 0.0797
Golgi 0.0204 0.0287 0.0223 0.03 0.0353 0.0178 0.0153 0.0096 0.0193 0.0254 0.0203 0.0145 0.0155 0.0147 0.0183 0.008 0.0115 0.0138
Lipid Particles 0.0782 0.0248 0.0405 0.0049 0.0374 0.041 0.0316 0.0154 0.0238 0.0123 0.0235 0.015 0.0162 0.008 0.0135 0.0054 0.0103 0.0135
Mitochondria 0.0528 0.0338 0.084 0.0547 0.0533 0.0778 0.0443 0.0543 0.0763 0.0703 0.0334 0.0438 0.0728 0.0489 0.0891 0.0415 0.0768 0.0448
None 0.4454 0.4218 0.4674 0.5868 0.3099 0.4137 0.5298 0.5592 0.5125 0.5 0.4248 0.5887 0.4409 0.406 0.4383 0.5075 0.4183 0.4516
Nuclear Periphery 0.0088 0.0012 0.0015 0.0009 0.0051 0.0019 0.0144 0.0018 0.0043 0.002 0.0064 0.001 0.0033 0.0007 0.0015 0.0112 0.0017 0.0015
Nucleolus 0.0026 0.001 0.0012 0.0006 0.0014 0.0012 0.0017 0.0023 0.0022 0.0019 0.0012 0.0015 0.0021 0.0013 0.0022 0.001 0.0007 0.0011
Nucleus 0.0034 0.0009 0.0015 0.0008 0.0021 0.0024 0.0101 0.0035 0.0049 0.002 0.0019 0.0028 0.0036 0.0034 0.0037 0.0038 0.002 0.0037
Peroxisomes 0.0342 0.0448 0.0285 0.0333 0.0505 0.0558 0.0358 0.0197 0.0526 0.1045 0.0891 0.0409 0.0235 0.0444 0.0324 0.0503 0.0452 0.0327
Punctate Nuclear 0.0093 0.003 0.0038 0.0056 0.0033 0.0042 0.0054 0.007 0.0045 0.0051 0.0085 0.0112 0.0082 0.0023 0.005 0.006 0.0023 0.0095
Vacuole 0.0088 0.005 0.0048 0.003 0.0202 0.0039 0.0067 0.0078 0.0054 0.0039 0.006 0.0042 0.0101 0.0135 0.0121 0.0114 0.0058 0.0052
Vacuole Periphery 0.0024 0.0011 0.0015 0.0015 0.0049 0.0019 0.0024 0.0016 0.002 0.0023 0.0022 0.0013 0.002 0.0018 0.0028 0.0035 0.0018 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6631 3.3356 10.6077 7.0397 8.9845 4.262 8.311 9.1051 8.4022 6.093
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1580 1750 1522 1557 1201 2169 117 2557 2781 3919 1639 4114

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 594.47 703.20 881.19 819.35 634.84 676.44 880.15 859.67 611.90 688.39 881.12 844.41
Standard Deviation 66.28 89.98 726.53 120.28 70.92 87.51 90.99 130.03 71.19 89.61 700.54 127.93
Intensity Change Log 2 — 0.242331 0.567849 0.462876 — 0.091569 0.471356 0.437390 — 0.166443 0.518825 0.449771

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000247 0.001160 0.001913 0.009922 0.000499 0.000775 0.001469 0.002132 0.000356 0.000947 0.001881 0.005080
Bud Neck 0.063200 0.062942 0.020110 0.039897 0.046813 0.056413 0.007006 0.079192 0.056123 0.059328 0.019175 0.064320
Bud Site 0.012980 0.036644 0.024965 0.117829* 0.012677 0.028168 0.008480 0.071183 0.012849 0.031953 0.023788 0.088837
Cell Periphery 0.000237 0.000116 0.000370 0.000284 0.000196 0.000173 0.000315 0.000108 0.000220 0.000147 0.000366 0.000175
Cytoplasm 0.236724* 0.165844* 0.205252* 0.296204* 0.189001* 0.119546* 0.183061* 0.367932* 0.216114* 0.140220* 0.203668* 0.340786*
Cytoplasmic Foci 0.325103* 0.268422* 0.040433 0.084426 0.370157* 0.269680* 0.109659 0.053466 0.344560* 0.269118* 0.045375 0.065183
Eisosomes 0.000292 0.000134 0.000096 0.000130 0.000271 0.000164 0.000238 0.000059 0.000283 0.000151 0.000106 0.000086
Endoplasmic Reticulum 0.001523 0.001452 0.005666 0.004781 0.001236 0.000715 0.008217 0.000994 0.001399 0.001044 0.005848 0.002427
Endosome 0.049277 0.053505 0.006665 0.015276 0.047776 0.082037 0.039813 0.009989 0.048628 0.069296 0.009031 0.011990
Golgi 0.013166 0.028591 0.003912 0.010351 0.024085 0.043052 0.008230 0.004675 0.017881 0.036595 0.004220 0.006823
Lipid Particles 0.029647 0.007765 0.004108 0.003380 0.024191 0.012105 0.016339 0.002000 0.027291 0.010167 0.004981 0.002523
Mitochondria 0.066643 0.119099* 0.018577 0.014605 0.083265 0.175218* 0.000981 0.040179 0.073821 0.150158* 0.017321 0.030500
Mitotic Spindle 0.003287 0.034298 0.026798 0.107669* 0.007572 0.032937 0.001786 0.116494* 0.005138 0.033545 0.025013 0.113154*
None 0.006150 0.002333 0.006526 0.002161 0.002208 0.001787 0.003054 0.003339 0.004448 0.002031 0.006279 0.002893
Nuclear Periphery 0.000679 0.000585 0.004780 0.001402 0.001028 0.000534 0.002651 0.001768 0.000829 0.000557 0.004628 0.001629
Nuclear Periphery Foci 0.000448 0.000345 0.001243 0.001240 0.000504 0.000326 0.003904 0.000287 0.000472 0.000335 0.001433 0.000648
Nucleolus 0.005252 0.003776 0.006397 0.001396 0.002835 0.002346 0.009701 0.002610 0.004208 0.002984 0.006632 0.002150
Nucleus 0.094398 0.056326 0.575744* 0.106639 0.079285 0.030879 0.361662* 0.109802 0.087871 0.042242 0.560462* 0.108605
Peroxisomes 0.030494 0.061570 0.006710 0.052845 0.060064 0.059860 0.027888 0.029550 0.043264 0.060624 0.008221 0.038366
Vacuole 0.054159 0.087656 0.036047 0.126163 0.041666 0.073158 0.204328* 0.101527 0.048764 0.079632 0.048060 0.110851
Vacuole Periphery 0.006093 0.007436 0.003688 0.003403 0.004672 0.010126 0.001217 0.002712 0.005479 0.008924 0.003512 0.002973

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.51 -3.64 -9.00 -7.66 -6.12 -2.69 -2.50 -6.27 -4.57 -0.31 -6.36 -3.60 -10.83 -8.69 -4.00
Bud Neck 0.08 11.62 6.95 7.97 -6.19 -2.55 12.13 -6.60 -4.55 -25.98* -1.19 13.37 -0.97 0.30 -18.22
Bud Site -10.58 -4.49 -16.75 -10.64 -12.57 -8.43 1.73 -15.06 -8.53 -14.82 -13.39 -4.58 -22.44 -13.23 -13.19
Cell Periphery 8.62 -3.16 0.47 -7.52 3.31 1.21 -3.96 7.71 5.77 7.89 5.92 -3.69 7.20 0.38 5.32
Cytoplasm 8.60 4.39 -5.60 -14.20 -9.92 9.20 0.53 -20.28 -35.41 -9.92 13.61 2.54 -18.83 -35.80 -19.15
Cytoplasmic Foci 7.41 43.69 38.96 33.02 -7.03 12.42 20.25 46.68 44.44 4.48 13.55 57.89 61.16 55.56 -3.72
Eisosomes 8.67 9.74 9.21 1.64 -2.41 8.01 0.81 18.17 14.92 9.16 11.05 12.22 17.48 13.94 2.75
Endoplasmic Reticulum 0.13 -11.32 -5.10 -5.18 2.31 3.22 -7.56 -0.27 -5.92 7.59 1.38 -15.37 -4.23 -5.90 11.00
Endosome -1.08 17.46 15.36 17.18 -4.28 -9.19 2.30 15.25 26.97 4.02 -8.06 21.31 22.07 31.88 -0.53
Golgi -8.24 8.37 5.39 12.08 -2.25 -7.42 4.28 12.47 20.18 0.55 -11.76 12.35 13.95 23.76 -0.95
Lipid Particles 12.52 14.95 15.62 6.85 1.77 7.43 2.65 15.44 12.13 4.01 13.89 18.57 21.76 14.12 5.53
Mitochondria -8.21 11.48 14.09 22.22 2.44 -12.57 16.11 10.01 26.51 -19.40* -15.75 15.95 15.66 33.69 -3.39
Mitotic Spindle -11.46 -8.47 -16.50 -9.43 -10.53 -8.87 3.26 -20.66 -13.64 -23.36* -14.65 -7.67 -27.12 -16.60 -16.83
None 3.62 0.25 4.01 0.32 5.69 0.95 -0.70 -2.18 -3.69 -0.96 3.90 -1.43 2.83 -2.54 4.74
Nuclear Periphery 0.50 -12.13 -9.56 -10.65 7.19 1.49 -3.23 -4.50 -10.82 -0.36 1.61 -11.43 -8.99 -14.57 6.90
Nuclear Periphery Foci 1.54 -5.73 -5.38 -5.95 1.77 1.92 -6.95 1.70 -0.49 7.26 2.48 -6.79 -3.25 -5.35 5.57
Nucleolus 1.68 -1.78 5.72 4.69 7.07 1.21 -1.39 -0.43 -2.16 1.34 2.48 -3.25 3.94 1.47 5.86
Nucleus 7.59 -50.87 -7.92 -14.83 43.51 10.39 -9.23 -11.38 -27.19 6.93 13.62 -52.78 -13.65 -29.53 46.51
Peroxisomes -9.21 12.57 -1.90 6.11 -10.43 0.00 4.94 10.24 12.64 -0.01 -6.75 18.28 6.64 13.71 -12.27
Vacuole -8.67 1.66 -22.93 -16.73 -24.23 -9.54 -9.42 -25.25 -16.85 4.84 -12.01 -4.14 -32.81 -23.23 -23.79
Vacuole Periphery -1.63 3.57 5.75 7.10 1.25 -8.60 9.85 7.19 14.18 -3.18 -6.81 3.58 9.14 15.43 1.59
Standard name
Human Ortholog
Description Double-stranded DNA-dependent helicase of the DExH/D-box family; required for maintenance of the mitochondrial (mt) genome; null mutant accumulates double-stranded breaks in mt DNA; localizes to the mt matrix
Localization
Cell Percentages mitochondrion (28%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Irc3

Irc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Irc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available