Standard name
Description Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.06 0.1 0.07 0.09 0.1 0.1 0.11 – 0.05 0.07 0.11 0.17 0.17 – – 0.29 0 0 0 0 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.12 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.32 0.58 0.66 0.39 0.71 0.7 0.75 0.75 – 0.86 0.84 0.12 0 0.22 – – 0.12 0 0 0.06 0 0 0
Nucleus 0.92 0.69 0.61 0.64 0.71 0.57 0.5 0.45 0.43 – 0.47 0.46 0.82 0.78 0.71 – – 0.59 0.56 0.63 0.54 0.65 0.56 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 – – 0.06 0.19 0.08 0.11 0.12 0.11 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.07 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.12 0.11 0.14 0.15 0.09 0.15 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 – – 0 0 1 0 0 0 1
Bud 0 0 0 1 2 2 6 12 4 – 6 9 0 0 5 – – 1 0 5 0 2 6 7
Bud Neck 1 0 1 0 1 0 2 1 1 – 2 0 0 0 0 – – 0 1 2 3 1 1 3
Bud Site 6 0 0 4 4 7 17 12 8 – 17 10 0 0 3 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 2 30 9 13 16 20 37 40 20 – 15 19 16 45 52 – – 5 1 7 3 1 16 6
Endoplasmic Reticulum 0 2 1 0 0 0 1 0 0 – 0 0 0 1 0 – – 1 0 1 0 2 0 1
Endosome 0 2 0 1 4 0 0 0 0 – 0 0 0 1 0 – – 2 10 11 4 2 2 7
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 1 3 0 0 0 2
Mitochondria 6 107 93 86 92 165 268 304 139 – 242 227 18 8 68 – – 2 2 8 9 1 7 7
Nucleus 167 228 97 84 165 132 192 182 79 – 132 123 122 211 218 – – 10 119 243 90 107 152 143
Nuclear Periphery 0 2 0 0 1 0 2 1 0 – 1 0 0 0 0 – – 0 1 2 0 0 0 2
Nucleolus 15 11 1 1 7 3 5 8 4 – 4 10 4 8 15 – – 1 40 30 17 20 29 54
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 1
SpindlePole 4 2 9 9 10 3 16 10 3 – 4 4 2 5 1 – – 1 7 10 7 7 11 25
Vac/Vac Membrane 1 12 0 1 2 4 1 5 2 – 2 8 3 9 8 – – 2 24 53 24 15 39 37
Unique Cell Count 182 331 159 131 233 232 381 408 185 282 269 148 270 307 17 215 386 168 166 272 308
Labelled Cell Count 202 397 211 200 304 336 547 576 260 425 410 165 288 370 25 215 386 168 166 272 308


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.1 3.5 3.7 3.8 3.3 3.2 3.1 2.8 – 2.8 2.6 4.9 5.1 4.2 – – 6.3 4.9 4.2 5.0
Std Deviation (1e-4) 0.8 1.0 1.0 1.8 1.6 1.1 1.2 1.0 0.9 – 0.9 0.9 1.1 1.2 0.9 – – 2.1 1.5 1.0 1.3
Intensity Change (Log2) – – – 0.09 0.13 -0.1 -0.13 -0.16 -0.34 – -0.31 -0.41 0.48 0.54 0.27 – – 0.83 0.47 0.27 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available