| Standard name | |
|---|---|
| Description | Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
| Bud | 0 | 0 | 0 | 1 | 2 | 2 | 6 | 12 | 4 | – | 6 | 9 | 0 | 0 | 5 | – | – | 1 | 0 | 5 | 0 | 2 | 6 | 7 |
| Bud Neck | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | – | 2 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 2 | 3 | 1 | 1 | 3 |
| Bud Site | 6 | 0 | 0 | 4 | 4 | 7 | 17 | 12 | 8 | – | 17 | 10 | 0 | 0 | 3 | – | – | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 2 | 30 | 9 | 13 | 16 | 20 | 37 | 40 | 20 | – | 15 | 19 | 16 | 45 | 52 | – | – | 5 | 1 | 7 | 3 | 1 | 16 | 6 |
| Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | – | 1 | 0 | 1 | 0 | 2 | 0 | 1 |
| Endosome | 0 | 2 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | – | 2 | 10 | 11 | 4 | 2 | 2 | 7 |
| Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 3 | 0 | 0 | 0 | 2 |
| Mitochondria | 6 | 107 | 93 | 86 | 92 | 165 | 268 | 304 | 139 | – | 242 | 227 | 18 | 8 | 68 | – | – | 2 | 2 | 8 | 9 | 1 | 7 | 7 |
| Nucleus | 167 | 228 | 97 | 84 | 165 | 132 | 192 | 182 | 79 | – | 132 | 123 | 122 | 211 | 218 | – | – | 10 | 119 | 243 | 90 | 107 | 152 | 143 |
| Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 2 | 0 | 0 | 0 | 2 |
| Nucleolus | 15 | 11 | 1 | 1 | 7 | 3 | 5 | 8 | 4 | – | 4 | 10 | 4 | 8 | 15 | – | – | 1 | 40 | 30 | 17 | 20 | 29 | 54 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| SpindlePole | 4 | 2 | 9 | 9 | 10 | 3 | 16 | 10 | 3 | – | 4 | 4 | 2 | 5 | 1 | – | – | 1 | 7 | 10 | 7 | 7 | 11 | 25 |
| Vac/Vac Membrane | 1 | 12 | 0 | 1 | 2 | 4 | 1 | 5 | 2 | – | 2 | 8 | 3 | 9 | 8 | – | – | 2 | 24 | 53 | 24 | 15 | 39 | 37 |
| Unique Cell Count | 182 | 331 | 159 | 131 | 233 | 232 | 381 | 408 | 185 | 282 | 269 | 148 | 270 | 307 | 17 | 215 | 386 | 168 | 166 | 272 | 308 | |||
| Labelled Cell Count | 202 | 397 | 211 | 200 | 304 | 336 | 547 | 576 | 260 | 425 | 410 | 165 | 288 | 370 | 25 | 215 | 386 | 168 | 166 | 272 | 308 | |||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.9 | 4.1 | 3.5 | 3.7 | 3.8 | 3.3 | 3.2 | 3.1 | 2.8 | – | 2.8 | 2.6 | 4.9 | 5.1 | 4.2 | – | – | 6.3 | 4.9 | 4.2 | 5.0 |
| Std Deviation (1e-4) | 0.8 | 1.0 | 1.0 | 1.8 | 1.6 | 1.1 | 1.2 | 1.0 | 0.9 | – | 0.9 | 0.9 | 1.1 | 1.2 | 0.9 | – | – | 2.1 | 1.5 | 1.0 | 1.3 |
| Intensity Change (Log2) | – | – | – | 0.09 | 0.13 | -0.1 | -0.13 | -0.16 | -0.34 | – | -0.31 | -0.41 | 0.48 | 0.54 | 0.27 | – | – | 0.83 | 0.47 | 0.27 | 0.52 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
