Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.09 0 0 0 0.05 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 – 0 0.07 0 0 0 0 0 0
Cytoplasm 0.09 0.21 0.16 0.14 0.14 0.05 0.05 0.05 0 0.05 0 0 0.25 0.45 0.53 – 0.19 0.21 0 0 0 0 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.08 0 0 0 0 0 0
Endosome 0.06 0 0.21 0.12 0.06 0.09 0.07 0.12 0 0 0 0 0.25 0.15 0.13 – 0.29 0.24 0.13 0.08 0.09 0.09 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.77 0.78 0.28 0.73 0.75 0.79 0.79 0.74 0.94 0.91 0.91 0.93 0.35 0.18 0.15 – 0.1 0.12 0.72 0.74 0.74 0.65 0.6 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.14 0.16 0 0.06 0 0 0 0 0 0 0 0 0.16 – 0.28 0.14 0 0 0.05 0 0 0
SpindlePole 0 0 0.28 0.07 0.11 0.07 0.09 0.12 0 0.08 0.05 0.07 0.05 0.08 0 – 0.17 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.06 0.09 0 0.06 0.12 0.07 0.09 0 0 0.05 0 0.07 0.11 0.1 – 0.16 0.23 0 0.05 0 0.05 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 4 2 4 1 0 0 0 1 0 0 2 3 1 – 0 0 1 9 8 2 4 4
Bud 2 12 10 6 22 10 18 10 24 20 24 28 1 1 1 – 3 2 0 2 1 1 1 2
Bud Neck 1 4 3 0 4 0 0 1 1 2 0 3 0 0 0 – 0 0 1 2 1 2 3 1
Bud Site 0 2 0 0 1 3 3 2 9 6 10 12 0 0 0 – 0 1 – – – – – –
Cell Periphery 2 9 3 3 1 4 2 4 1 0 3 2 4 3 0 – 2 7 0 0 1 0 0 0
Cytoplasm 13 64 40 32 33 18 27 25 20 23 13 25 14 55 77 – 22 21 1 11 2 5 9 5
Endoplasmic Reticulum 6 14 0 1 0 1 5 3 8 4 4 3 1 3 4 – 7 8 0 2 2 1 0 1
Endosome 9 1 53 28 14 34 36 53 1 1 13 1 14 19 19 – 34 24 23 29 35 14 13 16
Golgi 2 1 0 0 0 2 3 2 0 0 2 0 1 1 0 – 1 1 4 1 7 0 3 1
Mitochondria 115 243 73 169 180 289 415 340 463 424 485 617 20 22 22 – 12 12 128 276 294 108 115 131
Nucleus 1 2 1 1 5 9 11 10 12 16 7 10 0 1 1 – 3 3 0 0 0 2 6 5
Nuclear Periphery 4 0 0 0 0 6 14 6 9 10 8 12 0 0 0 – 0 0 1 1 0 0 0 2
Nucleolus 0 0 1 1 0 5 5 2 8 4 7 5 0 0 0 – 0 1 0 0 2 1 4 1
Peroxisomes 3 0 35 36 6 21 16 10 1 7 14 9 0 4 23 – 33 14 5 7 21 5 6 4
SpindlePole 3 9 71 17 27 25 49 56 17 38 29 49 3 10 5 – 20 19 1 2 5 5 6 6
Vac/Vac Membrane 27 18 23 8 14 42 38 43 21 11 27 25 4 13 14 – 18 23 5 16 6 8 11 13
Unique Cell Count 149 312 258 230 241 365 523 457 495 468 531 663 57 123 144 116 98 180 373 399 166 191 205
Labelled Cell Count 189 384 317 304 311 470 642 567 595 567 646 801 64 135 167 155 136 180 373 399 166 191 205


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.5 5.5 5.2 4.8 5.1 4.9 5.2 4.0 4.2 4.4 4.3 5.7 5.3 5.1 4.9 6.1 5.8 5.6 6.2 6.5
Std Deviation (1e-4) 0.6 0.5 1.3 1.4 1.4 1.5 1.3 1.5 1.2 1.1 1.1 1.0 1.7 1.4 1.4 2.3 2.5 1.9 1.5 1.7 2.1
Intensity Change (Log2) – – – -0.06 -0.18 -0.09 -0.16 -0.08 -0.45 -0.38 -0.32 -0.35 0.06 -0.04 -0.09 -0.17 0.15 0.08 0.05 0.19 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 1.9 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0111 6.1984 6.1005 6.2475 5.3265 6.2604 6.7229 7.5102 6.7502 6.2936 6.5698 7.13 5.3323 3.8487 3.7621 3.6863 3.3163 3.5039
Actin 0.0623 0.0229 0.0339 0.0061 0.0046 0.0264 0.027 0.0095 0.0102 0.0033 0.0175 0.0032 0.0949 0.0079 0.029 0.0927 0.177 0.0109
Bud 0.0007 0.0025 0.0006 0.0015 0.0003 0.0003 0.0005 0.0006 0.0007 0.0002 0.0002 0.0008 0.0023 0.0012 0.0012 0.0002 0.0008 0.0002
Bud Neck 0.0023 0.0003 0.0013 0.0002 0.0001 0.0014 0.0004 0.0003 0.0001 0.0002 0.0002 0.0002 0.0029 0.0016 0.0006 0.0001 0.0004 0.001
Bud Periphery 0.0013 0.0025 0.0014 0.0105 0.0007 0.0005 0.0011 0.001 0.0035 0.0013 0.0003 0.0014 0.004 0.0019 0.0051 0.0004 0.0012 0.0005
Bud Site 0.0083 0.0076 0.0043 0.001 0.0003 0.0045 0.0012 0.0028 0.0016 0.0006 0.0005 0.0007 0.0205 0.0316 0.0041 0.0005 0.0012 0.0006
Cell Periphery 0.0005 0.0005 0.0015 0.0003 0.0003 0.0001 0.0002 0.0002 0.0019 0.0033 0 0.0012 0.0008 0.0045 0.0005 0.0002 0.0002 0.0005
Cytoplasm 0.0022 0.0036 0.0011 0.0006 0.0006 0.0003 0.0037 0.0006 0.0007 0.0002 0 0.0003 0.0143 0.003 0.0256 0.0152 0.0005 0.0039
Cytoplasmic Foci 0.0389 0.0221 0.0242 0.0241 0.0181 0.0082 0.0171 0.0401 0.0027 0.0038 0.0062 0.0044 0.0559 0.0699 0.0296 0.0018 0.0063 0.0096
Eisosomes 0.0007 0.0003 0.0013 0.0002 0.0003 0.0003 0.0003 0.0002 0.0018 0.0035 0.0002 0.0007 0.0025 0.0018 0.0003 0.001 0.0005 0.0019
Endoplasmic Reticulum 0.0119 0.0067 0.0011 0.0002 0.0004 0.0002 0.0047 0.0005 0.0002 0.0001 0 0.0002 0.0037 0.0004 0.0011 0.0023 0.0027 0.0019
Endosome 0.0654 0.0253 0.0315 0.0224 0.0577 0.0193 0.0591 0.0342 0.0069 0.0095 0.0049 0.0143 0.0338 0.0279 0.0227 0.0018 0.0159 0.0106
Golgi 0.0985 0.0543 0.0389 0.0506 0.0669 0.0604 0.033 0.0665 0.0088 0.0396 0.0132 0.0329 0.0234 0.0325 0.0231 0.0031 0.0382 0.0198
Lipid Particles 0.0337 0.0101 0.0133 0.0086 0.0449 0.0083 0.0318 0.0096 0.0046 0.0118 0.0103 0.0192 0.1068 0.0242 0.0123 0.0017 0.0103 0.028
Mitochondria 0.5722 0.7956 0.7923 0.8498 0.7728 0.8237 0.7481 0.6775 0.9215 0.9065 0.8473 0.8929 0.4864 0.6637 0.7332 0.7957 0.7333 0.8396
None 0.009 0.0007 0.0097 0.0007 0.0003 0.0002 0.0061 0.0001 0.0013 0.0001 0.0001 0.0013 0.0383 0.0446 0.0081 0.0777 0.0003 0.0062
Nuclear Periphery 0.0057 0.0012 0.0004 0.0001 0.0004 0.0003 0.0134 0.0001 0 0 0 0.0001 0.0116 0.0003 0.0004 0.0009 0.0012 0.0013
Nucleolus 0.0011 0.0003 0.0004 0.0001 0.0003 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0003 0.0014 0.0019 0.0004 0.0002 0.0001 0.0001
Nucleus 0.0007 0.0003 0.0003 0.0001 0.0002 0.0001 0.002 0.0001 0.0001 0.0001 0 0.0002 0.0035 0.0004 0.0005 0.0006 0.0001 0.0004
Peroxisomes 0.0739 0.0319 0.0357 0.0204 0.0223 0.0381 0.0355 0.1528 0.0306 0.014 0.098 0.0216 0.0579 0.0535 0.0982 0.0027 0.0074 0.0577
Punctate Nuclear 0.0009 0.0001 0.0029 0.0008 0.0003 0.002 0.0058 0.0001 0 0 0 0 0.0296 0.0003 0.0004 0.0006 0.0007 0.0001
Vacuole 0.0049 0.0081 0.0021 0.0007 0.0019 0.0019 0.0037 0.0012 0.0011 0.0006 0.0003 0.0018 0.0017 0.0151 0.0029 0.0005 0.0006 0.0012
Vacuole Periphery 0.0048 0.0032 0.0019 0.0011 0.0065 0.0033 0.0044 0.0017 0.0013 0.001 0.0007 0.0023 0.0041 0.012 0.0006 0.0003 0.001 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.0192 14.893 22.1025 25.3872 22.4312 15.3699 22.8771 27.3629 30.8109 29.1609
Translational Efficiency 1.7131 1.559 1.1759 1.3255 1.2614 2.1134 1.4466 1.3795 1.3769 1.4624

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1676 877 1954 1541 1978 2044 204 1649 3654 2921 2158 3190

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.44 692.11 855.12 867.71 641.16 820.23 916.61 873.91 664.22 781.76 860.93 870.91
Standard Deviation 80.71 119.49 100.83 154.54 78.73 102.45 106.99 115.88 83.49 122.80 103.01 135.97
Intensity Change Log 2 — 0.001397 0.306523 0.327609 — 0.355344 0.515624 0.446800 — 0.182539 0.410915 0.386186

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001007 0.004732 0.006641 0.038618 0.000934 0.001130 0.004095 0.032242 0.000967 0.002211 0.006401 0.035322
Bud Neck 0.012046 0.019933 0.009231 0.008580 0.007876 0.007906 0.020473 0.006411 0.009789 0.011517 0.010293 0.007459
Bud Site 0.005852 0.025016 0.033208 0.102722* 0.006290 0.006698 0.021979 0.085573 0.006089 0.012198 0.032146 0.093857
Cell Periphery 0.000552 0.001516 0.000196 0.000887 0.000232 0.000223 0.000453 0.000778 0.000379 0.000612 0.000220 0.000831
Cytoplasm 0.014918 0.013447 0.016286 0.032936 0.008921 0.006463 0.016208 0.028227 0.011672 0.008560 0.016279 0.030502
Cytoplasmic Foci 0.123442 0.088746 0.114222 0.086519 0.084513 0.082978 0.126871 0.091201 0.102369 0.084710 0.115418 0.088940
Eisosomes 0.000327 0.000200 0.000114 0.000229 0.000115 0.000132 0.000184 0.000244 0.000212 0.000152 0.000120 0.000237
Endoplasmic Reticulum 0.000917 0.000620 0.004550 0.001768 0.000607 0.000490 0.003646 0.002042 0.000749 0.000529 0.004465 0.001910
Endosome 0.066604 0.105615 0.087502 0.069728 0.073059 0.092355 0.164263 0.072418 0.070099 0.096336 0.094758 0.071118
Golgi 0.171002* 0.156355* 0.128180* 0.116851* 0.143734* 0.198029* 0.076464 0.131171* 0.156241* 0.185517* 0.123291* 0.124253*
Lipid Particles 0.016496 0.006804 0.003741 0.000980 0.010696 0.011782 0.032046 0.001755 0.013356 0.010287 0.006417 0.001380
Mitochondria 0.497353* 0.453552* 0.318350* 0.348860* 0.593295* 0.519061* 0.206342* 0.375473* 0.549289* 0.499392* 0.307761* 0.362617*
Mitotic Spindle 0.003708 0.010207 0.111740* 0.043722 0.004793 0.008535 0.057868 0.039260 0.004295 0.009037 0.106647* 0.041415
None 0.000914 0.005684 0.000365 0.000392 0.000565 0.000503 0.000633 0.000221 0.000725 0.002058 0.000390 0.000304
Nuclear Periphery 0.000205 0.000190 0.001050 0.000099 0.000311 0.000148 0.002825 0.000093 0.000262 0.000161 0.001218 0.000096
Nuclear Periphery Foci 0.000192 0.000497 0.001494 0.000214 0.000299 0.000243 0.006817 0.000258 0.000250 0.000319 0.001998 0.000236
Nucleolus 0.000553 0.002746 0.001052 0.000153 0.001358 0.001040 0.008516 0.000149 0.000989 0.001552 0.001758 0.000151
Nucleus 0.002036 0.004283 0.009994 0.003737 0.001677 0.001242 0.023517 0.001174 0.001841 0.002155 0.011272 0.002412
Peroxisomes 0.059534 0.074521 0.047945 0.097471 0.034456 0.039677 0.115701* 0.083251 0.045959 0.050139 0.054350 0.090120
Vacuole 0.010504 0.012181 0.077710 0.035496 0.011615 0.010240 0.078327 0.034170 0.011105 0.010823 0.077768 0.034810
Vacuole Periphery 0.011839 0.013154 0.026430 0.010040 0.014652 0.011125 0.032772 0.013889 0.013362 0.011734 0.027029 0.012030

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -6.65 -12.36 -10.49 -10.09 -0.55 -2.29 -12.65 -12.56 -9.92 -3.09 -6.97 -17.62 -16.73 -13.76
Bud Neck -2.74 1.67 2.25 4.18 0.82 -0.03 -4.20 1.16 1.23 4.78 -1.35 -0.75 2.38 3.48 3.39
Bud Site -7.89 -16.31 -22.49 -15.94 -15.15 -0.40 -4.67 -21.56 -21.24 -12.92 -6.00 -17.02 -31.10 -27.80 -19.41
Cell Periphery -2.38 4.43 -2.01 1.41 -4.45 0.25 -2.45 -6.07 -6.28 -2.71 -1.83 3.84 -4.74 -1.53 -6.90
Cytoplasm 0.62 -1.43 -7.66 -7.54 -6.71 2.06 -1.80 -10.20 -12.43 -2.40 2.91 -3.96 -12.42 -14.75 -7.72
Cytoplasmic Foci 6.49 2.28 8.09 0.50 6.60 0.41 -4.66 -1.71 -2.12 3.90 5.73 -3.58 4.47 -1.40 7.64
Eisosomes 2.08 3.77 1.73 -1.07 -9.85 -1.76 -4.70 -9.64 -8.00 -3.01 2.18 3.44 -0.87 -6.99 -12.76
Endoplasmic Reticulum 1.45 -8.20 -3.32 -4.57 6.00 0.63 -5.10 -4.42 -4.67 2.46 1.53 -9.34 -5.57 -6.45 6.13
Endosome -6.91 -5.25 -0.66 6.44 4.55 -4.62 -7.29 0.17 4.96 7.37 -8.00 -7.07 -0.27 7.90 6.95
Golgi 1.80 6.91 8.18 5.16 1.76 -8.18 7.78 2.16 10.13 -6.27 -5.54 7.09 7.25 12.21 -0.46
Lipid Particles 5.07 8.65 11.04 4.89 6.09 -0.70 -2.98 7.72 7.99 4.27 2.27 5.71 13.31 9.49 6.29
Mitochondria 3.07 16.78 12.66 7.29 -2.79 6.90 19.13 19.22 12.70 -8.23 5.84 28.10 22.77 15.70 -6.27
Mitotic Spindle -4.42 -23.10* -13.10 -10.38 12.40 -3.66 -5.74 -12.64 -10.75 1.98 -5.50 -23.80 -18.21 -15.13 13.78
None -2.40 1.40 1.09 2.65 -1.06 0.61 -0.88 4.52 3.50 2.48 -2.16 1.73 2.09 2.89 1.22
Nuclear Periphery 0.03 -5.81 1.35 1.15 6.73 1.28 -2.58 1.76 1.39 2.82 1.24 -5.80 2.14 1.65 7.23
Nuclear Periphery Foci -2.34 -5.80 -0.76 2.02 5.62 0.52 -2.44 0.21 -0.46 2.45 -1.05 -5.42 -0.20 1.06 5.43
Nucleolus -2.94 -2.17 2.17 3.54 4.59 0.65 -3.14 2.54 2.80 3.70 -1.32 -2.21 3.07 4.49 5.73
Nucleus -1.92 -6.82 -2.92 -0.54 3.81 0.89 -4.74 0.92 0.06 4.83 -0.78 -8.19 -2.29 -1.44 6.83
Peroxisomes -2.44 2.85 -6.63 -3.15 -9.01 -1.63 -6.35 -10.36 -9.18 2.41 -1.40 -2.51 -12.04 -10.26 -8.53
Vacuole -1.10 -22.01 -10.52 -9.53 11.27 1.32 -7.76 -10.76 -11.57 4.93 0.32 -23.66 -15.02 -15.27 13.59
Vacuole Periphery -0.73 -9.10 1.69 1.84 10.58 2.98 -3.35 0.59 -2.30 3.50 1.75 -9.18 1.57 -0.26 10.24
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (90%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrps28

Mrps28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrps28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available