Standard name | |
---|---|
Human Ortholog | |
Description | Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 10 | 3 | 15 | 17 | 18 | 22 | – | 22 | 20 | 24 | 16 | 6 | 7 | 3 | 11 | 20 | 14 | 16 | 4 | 3 | 3 | 4 | 8 |
Bud | 3 | 2 | 0 | 3 | 5 | 8 | 13 | – | 26 | 10 | 16 | 11 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 1 | 1 | 2 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 2 | 0 | 5 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 4 | 8 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 2 | 0 | 2 | 4 | – | 5 | 1 | 5 | 4 | 3 | 6 | 2 | 3 | 15 | 12 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 145 | 100 | 105 | 51 | 42 | 28 | 32 | – | 20 | 21 | 18 | 22 | 103 | 213 | 248 | 74 | 113 | 103 | 101 | 80 | 62 | 32 | 81 | 74 |
Endoplasmic Reticulum | 11 | 22 | 4 | 8 | 13 | 28 | 8 | – | 9 | 9 | 5 | 6 | 0 | 6 | 3 | 4 | 10 | 8 | 7 | 6 | 3 | 1 | 2 | 2 |
Endosome | 8 | 8 | 7 | 12 | 58 | 66 | 100 | – | 55 | 58 | 20 | 50 | 2 | 16 | 28 | 8 | 12 | 13 | 55 | 26 | 9 | 5 | 20 | 22 |
Golgi | 2 | 9 | 1 | 8 | 25 | 34 | 40 | – | 25 | 29 | 28 | 33 | 0 | 2 | 9 | 22 | 48 | 20 | 11 | 16 | 1 | 0 | 2 | 3 |
Mitochondria | 45 | 7 | 1 | 15 | 40 | 130 | 136 | – | 179 | 167 | 270 | 200 | 0 | 0 | 1 | 5 | 4 | 5 | 54 | 24 | 48 | 6 | 20 | 28 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 4 | 2 | – | 2 | 5 | 3 | 3 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 6 | 6 | – | 13 | 14 | 21 | 15 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 6 | 0 | 8 | – | 1 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 8 | 1 | 4 | 10 | 32 | 13 | 31 | – | 20 | 21 | 12 | 23 | 0 | 1 | 3 | 2 | 1 | 7 | 13 | 3 | 3 | 0 | 4 | 2 |
Vac/Vac Membrane | 1 | 6 | 3 | 3 | 30 | 16 | 30 | – | 39 | 39 | 26 | 30 | 1 | 4 | 10 | 19 | 28 | 26 | 7 | 21 | 13 | 2 | 8 | 10 |
Unique Cell Count | 213 | 151 | 124 | 112 | 233 | 297 | 354 | 321 | 314 | 362 | 325 | 112 | 240 | 295 | 131 | 196 | 175 | 284 | 199 | 155 | 56 | 160 | 167 | |
Labelled Cell Count | 236 | 166 | 130 | 128 | 275 | 354 | 434 | 417 | 403 | 458 | 419 | 117 | 256 | 310 | 151 | 251 | 208 | 284 | 199 | 155 | 56 | 160 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 7.8 | 6.7 | 6.3 | 7.6 | 7.3 | 7.7 | – | 7.3 | 7.7 | 7.4 | 8.1 | 7.2 | 8.5 | 9.4 | 15.3 | 15.5 | 14.2 | 5.4 | 6.9 | 7.4 |
Std Deviation (1e-4) | 0.6 | 1.4 | 0.9 | 1.2 | 1.3 | 1.0 | 1.2 | – | 1.5 | 1.5 | 1.9 | 1.9 | 1.3 | 1.3 | 1.6 | 3.6 | 3.9 | 3.0 | 0.9 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.2 | 0.12 | 0.21 | – | 0.14 | 0.21 | 0.16 | 0.28 | 0.12 | 0.35 | 0.5 | 1.2 | 1.22 | 1.09 | -0.29 | 0.05 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.2 | 1.9 | 1.6 | 1.6 | – | 1.8 | 1.7 | 1.8 | 1.2 | 0 | 0 | 0 | 2.1 | 2.6 | 2.1 |
Bud | 0 | 0 | 0 | 0 | – | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.5 |
Cytoplasm | -6.3 | -12.2 | -15.1 | -16.0 | – | -16.5 | -16.2 | -17.6 | -16.4 | 1.7 | 1.1 | -0.2 | -4.9 | -5.1 | -4.8 |
Endoplasmic Reticulum | 1.4 | 1.0 | 2.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 |
Endosome | 1.4 | 4.5 | 4.1 | 5.2 | – | 3.1 | 3.4 | -0.1 | 2.8 | 0 | 0.4 | 1.3 | 0.2 | 0.2 | 0.6 |
Golgi | 0 | 3.4 | 3.6 | 3.6 | – | 2.8 | 3.1 | 2.8 | 3.3 | 0 | 0 | 0 | 4.5 | 5.7 | 3.5 |
Mitochondria | 3.8 | 4.6 | 8.7 | 8.0 | – | 10.6 | 10.2 | 14.3 | 11.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.9 | 3.1 | 0.5 | 2.0 | – | 1.3 | 1.4 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.2 | 1.3 | 2.3 | – | 3.1 | 3.2 | 1.9 | 2.5 | 0 | 0 | 0 | 3.4 | 3.5 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.569 | 2.8717 | 2.4642 | 2.5876 | 2.0593 | 3.033 | 1.5205 | 2.5369 | 1.9555 | 1.993 | 1.4392 | 1.818 | 1.0317 | 1.8875 | 1.4266 | 1.0907 | 0.9563 | 1.4021 |
Actin | 0.0172 | 0.0024 | 0.0033 | 0.0207 | 0.001 | 0.0033 | 0.0225 | 0.0062 | 0.0042 | 0.0019 | 0.0013 | 0.005 | 0.0382 | 0.0027 | 0.0122 | 0.0075 | 0.052 | 0.0024 |
Bud | 0.0015 | 0.0043 | 0.0041 | 0.0025 | 0.0017 | 0.0008 | 0.0017 | 0.0015 | 0.0014 | 0.0063 | 0.0016 | 0.0005 | 0.0004 | 0.0006 | 0.0014 | 0.0017 | 0.0062 | 0.0047 |
Bud Neck | 0.0042 | 0.0009 | 0.0017 | 0.0009 | 0.0008 | 0.0022 | 0.0041 | 0.0005 | 0.002 | 0.0027 | 0.0006 | 0.0052 | 0.001 | 0.0004 | 0.0007 | 0.0011 | 0.0117 | 0.0023 |
Bud Periphery | 0.001 | 0.0064 | 0.0059 | 0.0043 | 0.0014 | 0.0007 | 0.0017 | 0.0014 | 0.0013 | 0.0045 | 0.0014 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.001 | 0.0044 | 0.0046 |
Bud Site | 0.0162 | 0.0155 | 0.0099 | 0.0049 | 0.0017 | 0.0007 | 0.0056 | 0.009 | 0.0094 | 0.0022 | 0.0023 | 0.0006 | 0.0031 | 0.0037 | 0.0072 | 0.0019 | 0.0308 | 0.0004 |
Cell Periphery | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0014 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 |
Cytoplasm | 0.3308 | 0.4855 | 0.4657 | 0.4084 | 0.4354 | 0.3937 | 0.4211 | 0.6474 | 0.6413 | 0.4926 | 0.6648 | 0.476 | 0.372 | 0.6114 | 0.5817 | 0.5545 | 0.4878 | 0.5039 |
Cytoplasmic Foci | 0.1445 | 0.1452 | 0.1392 | 0.1366 | 0.1305 | 0.1663 | 0.1412 | 0.1039 | 0.0769 | 0.085 | 0.0809 | 0.1637 | 0.1484 | 0.1223 | 0.094 | 0.0756 | 0.0709 | 0.12 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0134 | 0.0032 | 0.0046 | 0.0036 | 0.0065 | 0.0059 | 0.0193 | 0.0059 | 0.006 | 0.0028 | 0.0015 | 0.0058 | 0.0165 | 0.0037 | 0.0072 | 0.0038 | 0.0053 | 0.0065 |
Endosome | 0.294 | 0.2237 | 0.2141 | 0.2673 | 0.3423 | 0.2882 | 0.21 | 0.149 | 0.1569 | 0.2252 | 0.1115 | 0.2444 | 0.2834 | 0.1881 | 0.2167 | 0.2624 | 0.2202 | 0.2777 |
Golgi | 0.0392 | 0.0284 | 0.0286 | 0.024 | 0.0277 | 0.0528 | 0.0255 | 0.029 | 0.0243 | 0.0518 | 0.0112 | 0.0343 | 0.0416 | 0.0193 | 0.0278 | 0.0302 | 0.0353 | 0.0396 |
Lipid Particles | 0.009 | 0.0016 | 0.0033 | 0.0058 | 0.0007 | 0.0011 | 0.0225 | 0.0012 | 0.0051 | 0.0065 | 0.0064 | 0.0064 | 0.0168 | 0.0008 | 0.0022 | 0.002 | 0.0107 | 0.0011 |
Mitochondria | 0.0138 | 0.009 | 0.0077 | 0.0056 | 0.0063 | 0.0355 | 0.0103 | 0.0159 | 0.0057 | 0.0814 | 0.0027 | 0.0069 | 0.0061 | 0.001 | 0.0035 | 0.0188 | 0.0057 | 0.006 |
None | 0.0813 | 0.0477 | 0.0902 | 0.0728 | 0.0151 | 0.0314 | 0.0563 | 0.0177 | 0.031 | 0.01 | 0.101 | 0.0328 | 0.0358 | 0.0313 | 0.0324 | 0.0163 | 0.0225 | 0.0172 |
Nuclear Periphery | 0.0069 | 0.0014 | 0.0014 | 0.0029 | 0.0018 | 0.0006 | 0.0079 | 0.0006 | 0.001 | 0.0009 | 0.0007 | 0.0007 | 0.0083 | 0.0007 | 0.0011 | 0.0011 | 0.0013 | 0.0007 |
Nucleolus | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0025 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0027 | 0 |
Nucleus | 0.005 | 0.0022 | 0.0019 | 0.0021 | 0.0017 | 0.001 | 0.0087 | 0.0008 | 0.0017 | 0.0013 | 0.0008 | 0.0006 | 0.0021 | 0.0008 | 0.0007 | 0.0013 | 0.0024 | 0.0009 |
Peroxisomes | 0.0069 | 0.0035 | 0.0037 | 0.0089 | 0.0015 | 0.0039 | 0.0133 | 0.0016 | 0.0068 | 0.0027 | 0.005 | 0.0078 | 0.0057 | 0.0012 | 0.0015 | 0.0026 | 0.0051 | 0.0027 |
Punctate Nuclear | 0.0019 | 0.0025 | 0.0011 | 0.0072 | 0.0005 | 0.0005 | 0.0067 | 0.0004 | 0.0004 | 0.0009 | 0.0009 | 0.001 | 0.0102 | 0.0004 | 0.0006 | 0.0004 | 0.0006 | 0.0005 |
Vacuole | 0.0092 | 0.0127 | 0.0102 | 0.0157 | 0.016 | 0.0076 | 0.0163 | 0.0064 | 0.0174 | 0.0153 | 0.0038 | 0.0053 | 0.0061 | 0.0092 | 0.0062 | 0.0123 | 0.0184 | 0.0061 |
Vacuole Periphery | 0.0029 | 0.003 | 0.0029 | 0.0047 | 0.0072 | 0.0036 | 0.0033 | 0.0013 | 0.0032 | 0.0053 | 0.0013 | 0.0024 | 0.0022 | 0.0019 | 0.0023 | 0.0054 | 0.0044 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.9323 | 9.4277 | 8.3075 | 14.9822 | 4.7003 | 11.1992 | 10.7239 | 15.2801 | 18.261 | 12.966 |
Translational Efficiency | 1.2901 | 1.1282 | 1.7348 | 1.7978 | 2.6229 | 1.8544 | 1.8156 | 2.0 | 1.9531 | 1.792 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
331 | 199 | 1323 | 1037 | 1608 | 1266 | 446 | 1402 | 1939 | 1465 | 1769 | 2439 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 753.27 | 868.59 | 1257.15 | 1168.58 | 807.33 | 801.75 | 1198.43 | 1129.88 | 798.10 | 810.83 | 1242.35 | 1146.33 |
Standard Deviation | 99.84 | 123.27 | 183.83 | 198.32 | 101.02 | 115.42 | 171.07 | 181.29 | 102.85 | 118.74 | 182.49 | 189.69 |
Intensity Change Log 2 | — | 0.205508 | 0.738918 | 0.633518 | — | -0.010006 | 0.569915 | 0.484939 | — | 0.098041 | 0.653963 | 0.558567 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000991 | 0.001440 | 0.002609 | 0.001393 | 0.000965 | 0.000996 | 0.006466 | 0.001631 | 0.000969 | 0.001056 | 0.003582 | 0.001530 |
Bud Neck | 0.018626 | 0.016951 | 0.001853 | 0.004460 | 0.012054 | 0.014049 | 0.004663 | 0.003120 | 0.013176 | 0.014443 | 0.002562 | 0.003690 |
Bud Site | 0.036355 | 0.043084 | 0.041356 | 0.049287 | 0.028761 | 0.032409 | 0.060163 | 0.053067 | 0.030057 | 0.033859 | 0.046098 | 0.051460 |
Cell Periphery | 0.000223 | 0.000107 | 0.000156 | 0.000096 | 0.000123 | 0.000136 | 0.000088 | 0.000122 | 0.000140 | 0.000132 | 0.000139 | 0.000111 |
Cytoplasm | 0.101553* | 0.346884* | 0.082565 | 0.377597* | 0.239769* | 0.252605* | 0.130756* | 0.512290* | 0.216174* | 0.265412* | 0.094715 | 0.455022* |
Cytoplasmic Foci | 0.393322* | 0.383925* | 0.235050* | 0.210367* | 0.450484* | 0.421461* | 0.333627* | 0.206064* | 0.440726* | 0.416363* | 0.259903* | 0.207894* |
Eisosomes | 0.000157 | 0.000102 | 0.000017 | 0.000015 | 0.000143 | 0.000121 | 0.000032 | 0.000017 | 0.000146 | 0.000119 | 0.000021 | 0.000016 |
Endoplasmic Reticulum | 0.000878 | 0.000499 | 0.002142 | 0.000593 | 0.002027 | 0.000735 | 0.000602 | 0.000300 | 0.001831 | 0.000703 | 0.001753 | 0.000424 |
Endosome | 0.109693 | 0.051438 | 0.098473 | 0.081376 | 0.070471 | 0.092728 | 0.126145 | 0.055188 | 0.077166 | 0.087119 | 0.105450 | 0.066322 |
Golgi | 0.181684* | 0.022899 | 0.273891* | 0.021811 | 0.093019 | 0.052831 | 0.048706 | 0.020193 | 0.108155 | 0.048765 | 0.217117* | 0.020881 |
Lipid Particles | 0.013620 | 0.001908 | 0.003465 | 0.003606 | 0.008160 | 0.005746 | 0.004436 | 0.002773 | 0.009092 | 0.005225 | 0.003710 | 0.003127 |
Mitochondria | 0.044937 | 0.004504 | 0.002339 | 0.001284 | 0.017733 | 0.014994 | 0.000687 | 0.001757 | 0.022377 | 0.013569 | 0.001923 | 0.001556 |
Mitotic Spindle | 0.022895 | 0.007125 | 0.150197* | 0.050893 | 0.011677 | 0.012128 | 0.140487* | 0.027287 | 0.013592 | 0.011449 | 0.147749* | 0.037323 |
None | 0.000729 | 0.000162 | 0.000077 | 0.000116 | 0.000541 | 0.000629 | 0.000146 | 0.000093 | 0.000573 | 0.000566 | 0.000095 | 0.000103 |
Nuclear Periphery | 0.000080 | 0.000071 | 0.000620 | 0.000399 | 0.000110 | 0.000100 | 0.000237 | 0.000090 | 0.000105 | 0.000096 | 0.000524 | 0.000221 |
Nuclear Periphery Foci | 0.000447 | 0.000362 | 0.009906 | 0.001366 | 0.000552 | 0.000304 | 0.002152 | 0.000727 | 0.000534 | 0.000312 | 0.007951 | 0.000999 |
Nucleolus | 0.002319 | 0.000187 | 0.000045 | 0.000166 | 0.000393 | 0.000473 | 0.000195 | 0.000110 | 0.000721 | 0.000434 | 0.000083 | 0.000134 |
Nucleus | 0.003858 | 0.007444 | 0.000558 | 0.002624 | 0.006401 | 0.005469 | 0.001675 | 0.001749 | 0.005967 | 0.005737 | 0.000840 | 0.002121 |
Peroxisomes | 0.048783 | 0.018095 | 0.002729 | 0.009547 | 0.012298 | 0.016778 | 0.023642 | 0.010529 | 0.018526 | 0.016957 | 0.008002 | 0.010112 |
Vacuole | 0.011523 | 0.088942 | 0.070819 | 0.169486 | 0.035593 | 0.065770 | 0.110239 | 0.096698 | 0.031484 | 0.068918 | 0.080757 | 0.127646 |
Vacuole Periphery | 0.007327 | 0.003870 | 0.021132 | 0.013517 | 0.008727 | 0.009537 | 0.004856 | 0.006196 | 0.008488 | 0.008767 | 0.017028 | 0.009309 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.72 | -3.47 | -0.68 | 0.25 | 2.56 | -0.08 | -2.76 | -1.43 | -1.24 | 2.42 | -0.29 | -4.39 | -1.52 | -1.06 | 3.47 |
Bud Neck | 0.50 | 6.17 | 5.17 | 4.05 | -8.30 | -1.55 | 6.77 | 9.81 | 11.42 | 4.04 | -0.97 | 11.85 | 10.68 | 11.65 | -3.93 |
Bud Site | -0.86 | -1.19 | -2.11 | -0.92 | -1.68 | -1.36 | -6.24 | -8.40 | -6.32 | 1.48 | -1.47 | -6.71 | -8.67 | -6.35 | -1.25 |
Cell Periphery | 3.34 | 1.86 | 3.82 | 0.78 | 5.41 | -0.53 | 2.99 | 0.29 | 0.67 | -2.03 | 0.41 | -0.09 | 2.64 | 1.13 | 3.02 |
Cytoplasm | -12.04 | 1.36 | -21.07 | -1.92 | -28.39 | -1.39 | 8.05 | -25.79 | -23.24 | -27.91 | -5.70 | 16.55 | -28.48 | -20.71 | -46.12 |
Cytoplasmic Foci | 0.73 | 13.85 | 15.79 | 10.91 | 4.60 | 3.57 | 12.32 | 34.33 | 27.04 | 9.20 | 3.36 | 29.64 | 39.34 | 30.40 | 9.28 |
Eisosomes | 3.11 | 10.78 | 10.95 | 6.56 | 1.82 | 1.07 | 5.68 | 6.56 | 12.59 | 4.11 | 1.56 | 7.72 | 8.04 | 13.98 | 3.50 |
Endoplasmic Reticulum | 0.84 | -2.53 | 0.79 | -0.19 | 5.40 | 3.59 | 3.84 | 4.98 | 4.38 | 4.29 | 3.65 | 0.04 | 4.79 | 3.34 | 6.26 |
Endosome | 6.01 | 1.39 | 4.12 | -3.63 | 5.98 | -5.46 | -6.63 | 5.81 | 10.41 | 9.59 | -2.51 | -7.10 | 5.37 | 7.59 | 13.26 |
Golgi | 13.12 | -6.39 | 14.14 | 0.85 | 34.21* | 9.45 | 9.86 | 19.96 | 11.69 | 5.42 | 14.16 | -14.89 | 24.17 | 11.80 | 32.23* |
Lipid Particles | 5.23 | 4.45 | 4.27 | -4.33 | -0.67 | 2.58 | 3.36 | 6.62 | 3.63 | 3.06 | 4.42 | 7.12 | 7.81 | 2.92 | 1.70 |
Mitochondria | 6.90 | 7.62 | 7.84 | 1.77 | 2.52 | 1.55 | 13.36 | 11.17 | 8.87 | -1.25 | 4.79 | 13.97 | 14.04 | 9.67 | 1.02 |
Mitotic Spindle | 3.19 | -16.19 | -4.83 | -8.76 | 11.78 | -0.23 | -11.17 | -5.55 | -5.14 | 9.27 | 1.09 | -23.08 | -8.94 | -9.35 | 16.98 |
None | 3.90 | 4.59 | 4.26 | 0.79 | -2.84 | -0.61 | 5.45 | 7.86 | 3.48 | 0.58 | 0.11 | 9.76 | 9.18 | 3.51 | -1.12 |
Nuclear Periphery | 0.14 | -5.18 | -2.83 | -2.92 | 1.64 | 0.39 | -3.91 | -0.60 | -1.19 | 3.68 | 0.37 | -5.67 | -2.70 | -2.97 | 3.55 |
Nuclear Periphery Foci | 0.45 | -7.46 | -3.77 | -3.90 | 6.63 | 2.25 | -5.27 | -2.01 | -3.71 | 4.40 | 2.31 | -7.98 | -3.85 | -5.68 | 7.33 |
Nucleolus | 2.30 | 2.43 | 2.31 | 0.28 | -9.19 | -1.29 | 2.04 | 6.29 | 5.31 | 2.57 | 1.67 | 3.58 | 3.32 | 5.02 | -3.17 |
Nucleus | -2.92 | 4.25 | 0.74 | 3.53 | -3.26 | 1.03 | 6.65 | 6.30 | 3.81 | -0.25 | 0.18 | 11.12 | 5.60 | 4.22 | -4.14 |
Peroxisomes | 5.51 | 10.34 | 8.89 | 2.64 | -8.43 | -3.01 | -3.57 | 2.41 | 5.13 | 4.79 | 0.97 | 8.83 | 7.72 | 6.12 | -2.46 |
Vacuole | -10.19 | -18.02 | -27.75 | -8.93 | -15.92 | -9.09 | -11.13 | -17.39 | -8.32 | 3.31 | -12.30 | -16.17 | -29.13 | -15.26 | -9.91 |
Vacuole Periphery | 2.42 | -7.74 | -2.14 | -4.91 | 5.06 | -0.86 | 4.74 | 3.66 | 3.85 | -1.13 | -0.27 | -8.12 | 0.51 | 0.67 | 7.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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