Standard name | |
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Human Ortholog | |
Description | Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 7 | 4 | 0 | 0 | 0 | 0 | – | 0 | 1 | 5 | 4 | 4 | 1 | – | 4 | 9 | 1 | 3 | 14 | 10 | 14 | 12 |
Bud | 0 | 3 | 1 | 4 | 2 | 6 | 5 | 13 | – | 11 | 20 | 14 | 0 | 0 | 1 | – | 0 | 2 | 0 | 1 | 2 | 2 | 3 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 2 | 2 | 3 | 3 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 12 | 13 | 11 | 9 | 16 | 9 | – | 19 | 14 | 23 | 10 | 18 | 26 | – | 10 | 16 | 2 | 2 | 6 | 9 | 12 | 24 |
Cytoplasm | 44 | 66 | 128 | 89 | 141 | 196 | 218 | 300 | – | 246 | 189 | 262 | 66 | 53 | 107 | – | 112 | 56 | 13 | 26 | 18 | 13 | 19 | 42 |
Endoplasmic Reticulum | 77 | 84 | 100 | 41 | 35 | 44 | 27 | 33 | – | 27 | 15 | 66 | 135 | 115 | 127 | – | 3 | 3 | 144 | 134 | 309 | 263 | 340 | 390 |
Endosome | 0 | 1 | 11 | 6 | 7 | 5 | 7 | 4 | – | 0 | 1 | 2 | 1 | 0 | 0 | – | 2 | 0 | 1 | 0 | 1 | 1 | 2 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 |
Mitochondria | 11 | 18 | 57 | 81 | 84 | 179 | 208 | 320 | – | 354 | 306 | 331 | 0 | 0 | 0 | – | 20 | 16 | 12 | 13 | 31 | 43 | 46 | 41 |
Nucleus | 2 | 1 | 0 | 0 | 1 | 2 | 2 | 10 | – | 2 | 4 | 9 | 1 | 5 | 2 | – | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 1 | 2 | 1 | 3 | 2 | 5 | 4 | – | 6 | 7 | 11 | 0 | 0 | 0 | – | 1 | 0 | 5 | 0 | 3 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | – | 2 | 1 | 0 | 0 | 0 | 1 | – | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 11 | 45 | 21 | 51 | 31 | 68 | 71 | – | 50 | 42 | 32 | 5 | 4 | 6 | – | 15 | 15 | 6 | 9 | 7 | 9 | 16 | 21 |
Unique Cell Count | 136 | 168 | 305 | 213 | 269 | 386 | 451 | 630 | 562 | 460 | 583 | 205 | 180 | 241 | 158 | 102 | 191 | 197 | 402 | 365 | 465 | 557 | ||
Labelled Cell Count | 160 | 193 | 364 | 262 | 337 | 477 | 557 | 768 | 721 | 603 | 757 | 222 | 199 | 271 | 177 | 123 | 191 | 197 | 402 | 365 | 465 | 557 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.1 | 5.2 | 5.5 | 4.9 | 4.3 | 4.4 | 4.4 | – | 3.7 | 3.8 | 3.8 | 7.2 | 7.2 | 7.7 | 4.7 | 5.6 | 6.8 | 7.2 | 7.7 | 7.8 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.3 | 1.6 | 1.4 | 1.2 | 1.7 | 1.1 | – | 0.9 | 0.8 | 0.7 | 1.1 | 1.0 | 1.1 | 0.9 | 1.0 | 1.2 | 1.5 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.09 | -0.28 | -0.24 | -0.24 | – | -0.49 | -0.47 | -0.45 | 0.46 | 0.46 | 0.55 | -0.17 | 0.11 | 0.37 | 0.46 | 0.56 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 1.1 | 0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.5 | 2.7 | 3.1 | – | 1.1 | 4.1 |
Cytoplasm | 0 | 2.5 | 2.3 | 1.7 | 1.6 | – | 0.5 | -0.2 | 0.8 | -2.2 | -2.8 | 0.6 | – | 5.9 | 2.3 |
Endoplasmic Reticulum | -3.4 | -5.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 7.3 | 6.7 | 4.7 | – | -7.6 | -6.0 |
Endosome | -0.5 | -0.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 4.9 | 3.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | -6.6 | -6.2 | -7.1 | – | -1.7 | -0.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -1.6 | 1.3 | -2.8 | 0.1 | -1.5 | – | -2.6 | -2.4 | -4.7 | -4.6 | -4.4 | -4.9 | – | -1.6 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3921 | 3.4908 | 3.5288 | 2.849 | 3.0547 | 3.4682 | 2.1085 | 2.9246 | 2.6067 | 2.6575 | 2.2794 | 2.7262 | 3.5284 | 3.1804 | 2.8 | 2.7011 | 3.5754 | 2.7912 |
Actin | 0.0105 | 0.002 | 0.0093 | 0.006 | 0.0184 | 0.0018 | 0.008 | 0.0011 | 0.0029 | 0.003 | 0.0014 | 0.0005 | 0.0434 | 0.0012 | 0.009 | 0.0012 | 0.0001 | 0.0006 |
Bud | 0.0026 | 0.009 | 0.0037 | 0.0027 | 0.0331 | 0.0029 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0034 | 0 | 0 | 0 |
Bud Neck | 0.0008 | 0.001 | 0.0055 | 0.0037 | 0.0028 | 0.0023 | 0.0004 | 0.0003 | 0.0009 | 0.0007 | 0.001 | 0.0006 | 0.0024 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0008 |
Bud Periphery | 0.0021 | 0.0032 | 0.0021 | 0.0025 | 0.0084 | 0.0018 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0043 | 0.0001 | 0 | 0 |
Bud Site | 0.0025 | 0.0387 | 0.0136 | 0.0023 | 0.0213 | 0.001 | 0.0014 | 0.0037 | 0.0079 | 0.0004 | 0 | 0 | 0.0017 | 0.0007 | 0.0031 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0019 | 0.002 | 0.0025 | 0.0011 | 0.0014 | 0.0008 | 0.0046 | 0.0024 | 0.0037 | 0.0011 | 0.0012 | 0.0005 | 0.0045 | 0.0058 | 0.003 | 0.0021 | 0.0002 | 0.0008 |
Cytoplasm | 0.223 | 0.3176 | 0.1992 | 0.2593 | 0.1755 | 0.3209 | 0.162 | 0.2312 | 0.1685 | 0.1608 | 0.231 | 0.1217 | 0.0924 | 0.2507 | 0.225 | 0.1337 | 0.0056 | 0.1861 |
Cytoplasmic Foci | 0.0102 | 0.0113 | 0.0176 | 0.0112 | 0.0127 | 0.0175 | 0.0133 | 0.0066 | 0.0097 | 0.0312 | 0.0071 | 0.0045 | 0.0026 | 0.0075 | 0.0076 | 0.0039 | 0 | 0.0021 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.6015 | 0.442 | 0.4815 | 0.4407 | 0.4537 | 0.4381 | 0.6977 | 0.6889 | 0.723 | 0.7239 | 0.6552 | 0.7584 | 0.7055 | 0.6579 | 0.6577 | 0.7824 | 0.9822 | 0.7694 |
Endosome | 0.0267 | 0.0238 | 0.0261 | 0.0231 | 0.0163 | 0.0167 | 0.0248 | 0.0114 | 0.0166 | 0.0126 | 0.0256 | 0.0172 | 0.0091 | 0.0134 | 0.0084 | 0.0058 | 0.0005 | 0.0085 |
Golgi | 0.0033 | 0.0012 | 0.0042 | 0.0028 | 0.0049 | 0.0008 | 0.0048 | 0.0012 | 0.0047 | 0.0113 | 0.0021 | 0.002 | 0.0158 | 0.0021 | 0.0026 | 0.0043 | 0 | 0.0046 |
Lipid Particles | 0.0086 | 0.0057 | 0.01 | 0.0134 | 0.0161 | 0.0037 | 0.0242 | 0.0119 | 0.0112 | 0.0047 | 0.0059 | 0.0077 | 0.0051 | 0.0151 | 0.0152 | 0.0212 | 0.0001 | 0.0027 |
Mitochondria | 0.0025 | 0.0014 | 0.0038 | 0.0025 | 0.0083 | 0.001 | 0.0006 | 0.0004 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.0036 | 0.0005 | 0.0014 | 0.0143 | 0 | 0.0014 |
None | 0.0471 | 0.0754 | 0.1084 | 0.1244 | 0.116 | 0.1274 | 0.0349 | 0.0167 | 0.0197 | 0.0097 | 0.0515 | 0.07 | 0.059 | 0.0246 | 0.0161 | 0.0023 | 0 | 0.016 |
Nuclear Periphery | 0.0286 | 0.0269 | 0.0396 | 0.0372 | 0.0379 | 0.021 | 0.0104 | 0.0081 | 0.0183 | 0.0104 | 0.01 | 0.0085 | 0.0262 | 0.0128 | 0.0097 | 0.0125 | 0.0095 | 0.004 |
Nucleolus | 0.0007 | 0.0014 | 0.0035 | 0.0035 | 0.0023 | 0.001 | 0.0001 | 0.0003 | 0.001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 |
Nucleus | 0.0105 | 0.0178 | 0.0318 | 0.023 | 0.0249 | 0.0154 | 0.0021 | 0.0055 | 0.0022 | 0.001 | 0.0014 | 0.0012 | 0.0148 | 0.0012 | 0.0023 | 0.0011 | 0.0007 | 0.0008 |
Peroxisomes | 0.0038 | 0.0003 | 0.0056 | 0.003 | 0.0201 | 0.0003 | 0.0026 | 0.0001 | 0.0004 | 0.0234 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0073 | 0.009 | 0 | 0.0001 |
Punctate Nuclear | 0.0033 | 0.0096 | 0.0219 | 0.0293 | 0.0183 | 0.0192 | 0.0011 | 0.001 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0049 | 0.0005 | 0.0183 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.008 | 0.0086 | 0.0085 | 0.0063 | 0.0058 | 0.0055 | 0.0062 | 0.0083 | 0.0068 | 0.0042 | 0.0048 | 0.0056 | 0.0054 | 0.005 | 0.0041 | 0.0045 | 0.001 | 0.0016 |
Vacuole Periphery | 0.0018 | 0.001 | 0.0014 | 0.0016 | 0.0016 | 0.0008 | 0.0005 | 0.0004 | 0.0007 | 0.0005 | 0.0005 | 0.0005 | 0.0009 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.3622 | 8.9671 | 21.7362 | 17.4831 | 25.1441 | 12.6988 | 15.7154 | 21.8256 | 23.9568 | 19.7319 |
Translational Efficiency | 1.2444 | 1.2334 | 0.7262 | 1.1598 | 0.6644 | 0.9144 | 0.9267 | 0.7782 | 0.7049 | 0.8351 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
77 | 429 | 1654 | 1170 | 57 | 1032 | 2447 | 978 | 134 | 1461 | 4101 | 2148 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1139.50 | 826.71 | 1013.08 | 1021.05 | 817.41 | 864.76 | 954.39 | 1012.47 | 1002.49 | 853.59 | 978.06 | 1017.14 |
Standard Deviation | 191.63 | 132.88 | 163.24 | 156.79 | 122.45 | 118.13 | 139.95 | 150.50 | 229.86 | 123.86 | 152.52 | 154.02 |
Intensity Change Log 2 | — | -0.462948 | -0.169653 | -0.158347 | — | 0.081240 | 0.223519 | 0.308747 | — | -0.210300 | 0.007764 | 0.055402 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000796 | 0.001131 | 0.001078 | 0.004738 | 0.000588 | 0.000526 | 0.001900 | 0.004501 | 0.000707 | 0.000704 | 0.001568 | 0.004630 |
Bud Neck | 0.004900 | 0.004525 | 0.003394 | 0.007911 | 0.002757 | 0.003351 | 0.004118 | 0.007098 | 0.003988 | 0.003696 | 0.003826 | 0.007541 |
Bud Site | 0.014855 | 0.007606 | 0.009217 | 0.031163 | 0.001208 | 0.004684 | 0.009368 | 0.023996 | 0.009050 | 0.005542 | 0.009307 | 0.027900 |
Cell Periphery | 0.001226 | 0.000868 | 0.000960 | 0.002977 | 0.005444 | 0.001675 | 0.002227 | 0.001939 | 0.003020 | 0.001438 | 0.001716 | 0.002504 |
Cytoplasm | 0.123078* | 0.215510* | 0.178527* | 0.170648* | 0.188319* | 0.196744* | 0.157754* | 0.178995* | 0.150830* | 0.202254* | 0.166132* | 0.174449* |
Cytoplasmic Foci | 0.069835 | 0.102391 | 0.012387 | 0.035639 | 0.069054 | 0.043268 | 0.012513 | 0.047306 | 0.069503 | 0.060629 | 0.012462 | 0.040951 |
Eisosomes | 0.000448 | 0.000560 | 0.000094 | 0.000950 | 0.001333 | 0.000899 | 0.000439 | 0.000687 | 0.000824 | 0.000799 | 0.000300 | 0.000830 |
Endoplasmic Reticulum | 0.161596* | 0.416249* | 0.599510* | 0.467230* | 0.515993* | 0.602129* | 0.700416* | 0.442104* | 0.312347* | 0.547548* | 0.659719* | 0.455790* |
Endosome | 0.040490 | 0.014235 | 0.008363 | 0.014463 | 0.011569 | 0.010258 | 0.005341 | 0.013754 | 0.028188 | 0.011426 | 0.006560 | 0.014140 |
Golgi | 0.021357 | 0.010881 | 0.001473 | 0.004757 | 0.009689 | 0.013665 | 0.002648 | 0.005391 | 0.016394 | 0.012848 | 0.002174 | 0.005046 |
Lipid Particles | 0.034663 | 0.064717 | 0.005960 | 0.033677 | 0.068913 | 0.044703 | 0.010497 | 0.036294 | 0.049232 | 0.050580 | 0.008667 | 0.034868 |
Mitochondria | 0.002311 | 0.007564 | 0.001290 | 0.002078 | 0.016630 | 0.007865 | 0.002696 | 0.001299 | 0.008402 | 0.007777 | 0.002129 | 0.001723 |
Mitotic Spindle | 0.003415 | 0.000245 | 0.001297 | 0.009822 | 0.000374 | 0.001062 | 0.000448 | 0.005060 | 0.002121 | 0.000822 | 0.000791 | 0.007654 |
None | 0.000050 | 0.000906 | 0.002095 | 0.000550 | 0.003545 | 0.001909 | 0.004930 | 0.001178 | 0.001537 | 0.001614 | 0.003786 | 0.000836 |
Nuclear Periphery | 0.010317 | 0.011257 | 0.025449 | 0.006323 | 0.010951 | 0.004756 | 0.002733 | 0.006063 | 0.010587 | 0.006665 | 0.011894 | 0.006204 |
Nuclear Periphery Foci | 0.046236 | 0.016701 | 0.046442 | 0.044345 | 0.009459 | 0.005980 | 0.013316 | 0.043954 | 0.030592 | 0.009128 | 0.026677 | 0.044167 |
Nucleolus | 0.000222 | 0.000835 | 0.000155 | 0.000270 | 0.000768 | 0.000190 | 0.000163 | 0.000210 | 0.000454 | 0.000379 | 0.000159 | 0.000243 |
Nucleus | 0.033507 | 0.072583 | 0.072145 | 0.041822 | 0.044246 | 0.028969 | 0.044767 | 0.043679 | 0.038075 | 0.041776 | 0.055809 | 0.042667 |
Peroxisomes | 0.002196 | 0.005363 | 0.000371 | 0.001303 | 0.003658 | 0.003170 | 0.000866 | 0.002179 | 0.002818 | 0.003814 | 0.000667 | 0.001702 |
Vacuole | 0.422819* | 0.044037 | 0.029179 | 0.117476 | 0.033292 | 0.021968 | 0.022289 | 0.132638 | 0.257124* | 0.028448 | 0.025068 | 0.124379 |
Vacuole Periphery | 0.005684 | 0.001835 | 0.000615 | 0.001859 | 0.002210 | 0.002230 | 0.000571 | 0.001674 | 0.004206 | 0.002114 | 0.000589 | 0.001775 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.95 | -1.96 | -5.02 | -4.43 | -4.56 | 0.22 | -3.99 | -5.01 | -5.35 | -3.25 | 0.07 | -4.67 | -7.04 | -7.13 | -5.36 |
Bud Neck | 0.42 | 1.10 | -2.66 | -4.03 | -5.62 | -0.41 | -1.25 | -3.63 | -3.93 | -4.07 | 0.39 | -0.04 | -4.30 | -5.43 | -6.69 |
Bud Site | 1.10 | 0.77 | -2.56 | -7.14 | -7.08 | -3.46 | -8.80 | -8.29 | -6.75 | -5.06 | 0.93 | -0.18 | -4.69 | -10.39 | -8.73 |
Cell Periphery | 0.71 | 0.38 | -3.17 | -4.60 | -4.34 | 0.90 | 0.76 | 0.86 | -0.37 | 0.89 | 0.84 | 0.68 | 0.32 | -2.79 | -1.96 |
Cytoplasm | -4.21 | -2.94 | -2.71 | 3.13 | 0.53 | -0.28 | 0.98 | 0.19 | 1.43 | -2.88 | -2.96 | -1.12 | -1.72 | 3.09 | -1.94 |
Cytoplasmic Foci | -2.84 | 6.30 | 3.60 | 9.15 | -9.70 | 1.64 | 3.58 | 1.27 | -1.49 | -12.37 | 1.04 | 6.72 | 3.19 | 5.39 | -15.68 |
Eisosomes | -0.88 | 2.89 | -2.19 | -1.66 | -4.31 | 0.54 | 1.12 | 0.84 | 1.28 | -2.19 | 0.04 | 1.49 | 0.05 | 0.02 | -4.35 |
Endoplasmic Reticulum | -8.34 | -16.00 | -9.81 | -0.62 | 11.44 | -1.60 | -3.32 | 2.12 | 12.10 | 21.03 | -7.40 | -10.94 | -3.43 | 10.20 | 23.95 |
Endosome | 3.59 | 4.54 | 3.61 | -0.06 | -5.52 | 0.23 | 1.11 | -0.36 | -2.45 | -8.41 | 3.34 | 4.43 | 2.79 | -2.45 | -10.23 |
Golgi | 0.81 | 1.79 | 1.49 | 4.09 | -3.79 | -0.79 | 1.43 | 0.96 | 5.55 | -2.16 | 0.34 | 2.15 | 1.74 | 7.27 | -3.87 |
Lipid Particles | -4.54 | 5.93 | -0.05 | 5.51 | -12.33 | 1.38 | 3.36 | 1.87 | 2.56 | -10.91 | -0.26 | 4.99 | 1.70 | 5.62 | -15.89 |
Mitochondria | -2.05 | 0.41 | 0.02 | 2.58 | -0.97 | 0.88 | 1.42 | 1.57 | 4.29 | 4.13 | 0.10 | 1.42 | 1.53 | 4.73 | 1.17 |
Mitotic Spindle | 2.12 | 1.38 | -2.19 | -4.73 | -4.07 | -1.17 | -0.29 | -3.00 | -2.51 | -2.99 | 1.49 | 1.49 | -3.16 | -5.07 | -5.16 |
None | -6.50 | -4.48 | -3.83 | 1.96 | 3.21 | 0.85 | -0.66 | 1.26 | 1.31 | 4.70 | -0.10 | -2.39 | 0.87 | 2.25 | 6.05 |
Nuclear Periphery | -0.48 | -4.21 | 0.84 | 1.89 | 9.89 | 2.00 | 2.71 | 1.58 | -1.31 | -4.50 | 1.34 | -0.75 | 1.58 | 0.43 | 6.12 |
Nuclear Periphery Foci | 3.42 | -0.37 | -0.04 | -8.92 | 0.75 | 1.52 | -1.80 | -10.07 | -13.91 | -10.78 | 4.16 | 0.44 | -2.90 | -17.53 | -7.78 |
Nucleolus | -2.84 | 0.67 | -0.96 | 2.54 | -2.52 | 1.02 | 1.07 | 0.95 | -1.12 | -2.46 | 0.21 | 1.17 | 0.74 | 1.68 | -3.34 |
Nucleus | -2.90 | -3.28 | -1.05 | 4.07 | 7.06 | 1.39 | -0.22 | -0.38 | -5.57 | -0.51 | -0.53 | -2.37 | -1.10 | -1.62 | 4.23 |
Peroxisomes | -2.79 | 2.63 | 1.33 | 4.65 | -4.56 | 0.49 | 3.18 | 1.52 | 1.79 | -3.69 | -1.44 | 3.93 | 1.96 | 4.94 | -5.20 |
Vacuole | 11.51 | 11.96 | 8.62 | -13.45 | -17.72 | 1.29 | 0.99 | -11.75 | -19.27 | -19.23 | 8.99 | 9.05 | 4.37 | -24.96 | -26.39 |
Vacuole Periphery | 1.44 | 1.99 | 1.47 | 0.25 | -2.35 | -0.03 | 1.99 | 0.67 | 1.05 | -1.46 | 1.28 | 2.45 | 1.61 | 1.17 | -2.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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