Standard name
Human Ortholog
Description GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 – 0.05 0 0.05 0 0.07 – – – 0 0 0 0 0 0
Cytoplasm 0.92 0.98 0.99 0.96 0.97 0.9 0.88 0.82 0.74 – 0.67 0.62 0.99 0.99 1.0 – – – 0.85 0.91 0.88 0.93 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0 0.21 0.2 0.32 0.42 – 0.5 0.53 0 0 0 – – – 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 0 2 0 1 0 1 0 – 3 3 0 0 0 – – – 3 3 4 0 1 1
Bud 1 0 3 1 7 2 5 13 7 – 7 7 1 0 8 – – – 0 0 0 4 1 4
Bud Neck 0 0 0 0 0 0 1 0 0 – 0 0 0 0 1 – – – 2 3 4 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 – 1 2 0 0 0 – – – – – – – – –
Cell Periphery 12 8 4 5 2 9 13 7 5 – 15 13 16 14 32 – – – 0 0 2 0 0 0
Cytoplasm 190 276 351 295 367 393 445 497 277 – 211 209 335 415 434 – – – 184 274 322 311 375 340
Endoplasmic Reticulum 7 2 1 0 3 2 3 2 7 – 6 10 0 0 2 – – – 5 1 4 3 8 11
Endosome 2 0 1 0 3 1 0 6 0 – 1 0 0 0 0 – – – 4 3 0 0 2 2
Golgi 2 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 3 0 0 0 2 3
Mitochondria 3 5 4 31 15 91 101 195 159 – 159 178 0 0 3 – – – 7 4 17 4 5 10
Nucleus 3 1 2 2 0 1 2 1 3 – 0 1 1 2 1 – – – 0 1 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 1 1 2 – 0 1 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 – – – 0 0 0 1 0 0
SpindlePole 0 0 0 1 0 0 1 2 0 – 0 3 0 1 0 – – – 0 0 0 0 1 2
Vac/Vac Membrane 1 0 0 0 4 6 4 13 5 – 1 6 2 0 0 – – – 2 7 5 4 6 4
Unique Cell Count 207 281 356 306 377 436 505 609 376 317 335 338 418 436 218 302 367 336 411 389
Labelled Cell Count 223 293 367 338 401 507 576 738 466 404 434 355 432 481 218 302 367 336 411 389


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.3 4.6 4.5 4.9 4.4 4.3 4.2 3.9 – 3.9 3.8 5.7 5.9 5.2 – – – 5.6 5.6 6.6
Std Deviation (1e-4) 0.8 0.7 0.9 1.0 1.1 1.0 1.1 1.0 1.1 – 1.0 1.2 1.0 1.2 1.0 – – – 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.05 0.08 -0.08 -0.12 -0.13 -0.25 – -0.24 -0.27 0.3 0.35 0.16 – – – 0.27 0.28 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 2.8 2.0 4.2 – – –
Cytoplasm -1.8 -1.2 -5.0 -5.7 -7.8 -9.7 – -11.2 -12.1 0.6 0.9 1.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Mitochondria 0 2.4 0 0 0 0 – 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4416 3.2672 3.3092 2.336 2.9144 2.9579 0.8264 0.746 1.1468 0.5683 -0.2413 1.0605 4.3731 4.9584 4.9929 5.4178 4.4412 4.9328
Actin 0.0588 0.0106 0.0198 0.0006 0.0045 0.0017 0.0286 0.002 0.0052 0.0093 0.0029 0.0188 0.0044 0.0071 0.004 0.0007 0.0024 0.0107
Bud 0.001 0.0041 0.0055 0.0003 0.0008 0.0002 0.0023 0.0013 0.0062 0.0112 0.0002 0.003 0.0005 0.0009 0.0069 0.0152 0.0207 0.0013
Bud Neck 0.0181 0.0005 0.0028 0.0004 0.0009 0.0328 0.0128 0.0005 0.0011 0.0011 0.0058 0.0174 0.0016 0.0012 0.001 0.0008 0.0493 0.0167
Bud Periphery 0.0027 0.003 0.0086 0.0005 0.0008 0.0003 0.0019 0.0018 0.0065 0.0177 0.0002 0.0061 0.0008 0.0008 0.0019 0.0102 0.0299 0.0011
Bud Site 0.0078 0.0146 0.0204 0.0006 0.0029 0.0002 0.0143 0.0097 0.0087 0.0036 0.0012 0.0113 0.0087 0.0344 0.0061 0.0026 0.0015 0.0008
Cell Periphery 0.0019 0.0027 0.0012 0.0012 0.0003 0.0009 0.0013 0.0011 0.0005 0.0006 0.0001 0.0005 0.0014 0.001 0.001 0.0003 0.0014 0.0006
Cytoplasm 0.5433 0.7759 0.7093 0.822 0.5455 0.7724 0.5421 0.7376 0.7103 0.7491 0.7845 0.7701 0.6204 0.6925 0.6829 0.762 0.711 0.7247
Cytoplasmic Foci 0.0222 0.0102 0.0322 0.007 0.0346 0.0152 0.0342 0.0219 0.0186 0.0171 0.0309 0.0141 0.0104 0.0132 0.0229 0.0216 0.0055 0.0153
Eisosomes 0.001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0141 0.0065 0.0043 0.0191 0.0047 0.0078 0.0158 0.0079 0.0064 0.0063 0.0037 0.0076 0.0049 0.0031 0.0055 0.005 0.0019 0.0029
Endosome 0.0417 0.0052 0.0133 0.0086 0.0315 0.0072 0.0244 0.0249 0.0195 0.0199 0.0203 0.0208 0.0064 0.0069 0.0067 0.0119 0.0028 0.0054
Golgi 0.0082 0.0017 0.0022 0.0008 0.0078 0.002 0.0026 0.0028 0.0023 0.0049 0.0032 0.006 0.0021 0.0012 0.0012 0.0008 0.0002 0.0009
Lipid Particles 0.0065 0.0016 0.0028 0.0006 0.0138 0.0022 0.002 0.0013 0.0016 0.0275 0.0108 0.0023 0.0018 0.0003 0.0009 0.0002 0.0002 0.0004
Mitochondria 0.0062 0.0014 0.0011 0.0006 0.0014 0.0006 0.0023 0.0011 0.005 0.004 0.0009 0.0013 0.0013 0.0006 0.0005 0.0008 0.0002 0.0004
None 0.2196 0.1511 0.1491 0.126 0.3313 0.1505 0.2419 0.1724 0.1763 0.0931 0.076 0.0984 0.3264 0.2289 0.248 0.1578 0.1697 0.2135
Nuclear Periphery 0.016 0.0017 0.0023 0.0013 0.004 0.0007 0.0241 0.0016 0.0079 0.0069 0.0011 0.0045 0.0014 0.0008 0.001 0.0007 0.0003 0.0004
Nucleolus 0.0009 0.0004 0.0003 0.0002 0.0006 0.0001 0.0015 0.0003 0.0008 0.0005 0.0034 0.0004 0.0005 0.0002 0.0003 0.0002 0.0001 0.0001
Nucleus 0.0162 0.0018 0.0117 0.0022 0.0018 0.0008 0.0248 0.0026 0.0073 0.0045 0.0028 0.0022 0.0015 0.0023 0.0018 0.0014 0.001 0.0008
Peroxisomes 0.0027 0.0005 0.0069 0.0001 0.0022 0.0018 0.0033 0.0008 0.0009 0.0117 0.0123 0.0011 0.0005 0.0006 0.0015 0.0011 0.0001 0.001
Punctate Nuclear 0.0042 0.0014 0.0034 0.0002 0.0076 0.0008 0.0078 0.0011 0.0057 0.0047 0.0363 0.0094 0.0014 0.001 0.0027 0.0012 0.0002 0.001
Vacuole 0.0047 0.0045 0.0024 0.007 0.0023 0.0017 0.0098 0.0066 0.0062 0.0045 0.0029 0.0039 0.0029 0.0027 0.0025 0.0047 0.0017 0.0014
Vacuole Periphery 0.0022 0.0004 0.0003 0.0007 0.0005 0.0002 0.0018 0.0008 0.0029 0.0017 0.0004 0.0007 0.0003 0.0002 0.0003 0.0005 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2832 7.8129 7.9716 13.8718 7.0822 10.1908 10.2036 13.3292 11.8282 11.9067
Translational Efficiency 0.7867 0.7607 1.0753 0.5964 1.0807 0.7722 0.8444 0.718 0.8745 0.7795

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1213 316 144 1014 1524 1610 750 2116 2737 1926 894 3130

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.38 745.76 976.16 852.39 656.44 745.23 862.41 838.29 644.45 745.32 880.73 842.86
Standard Deviation 85.97 103.60 105.66 118.27 131.26 110.74 129.61 107.37 114.23 109.60 132.81 111.21
Intensity Change Log 2 — 0.244780 0.633186 0.437582 — 0.183023 0.393711 0.352786 — 0.213582 0.515895 0.394915

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000153 0.000581 0.000559 0.002563 0.000192 0.000697 0.000074 0.001125 0.000175 0.000678 0.000152 0.001591
Bud Neck 0.024239 0.015525 0.007226 0.005775 0.012121 0.029479 0.002222 0.013872 0.017491 0.027190 0.003028 0.011249
Bud Site 0.014873 0.034285 0.018959 0.035597 0.010713 0.032250 0.001713 0.039246 0.012556 0.032584 0.004491 0.038064
Cell Periphery 0.000215 0.000116 0.000392 0.000260 0.000176 0.000153 0.000653 0.000133 0.000193 0.000147 0.000611 0.000174
Cytoplasm 0.524268* 0.460463* 0.468510* 0.541928* 0.579198* 0.491494* 0.572075* 0.631515* 0.554854* 0.486402* 0.555394* 0.602492*
Cytoplasmic Foci 0.221303* 0.286469* 0.088121 0.046201 0.203983* 0.229281* 0.039015 0.040506 0.211659* 0.238664* 0.046925 0.042351
Eisosomes 0.000216 0.000105 0.000144 0.000100 0.000137 0.000182 0.000668 0.000057 0.000172 0.000169 0.000584 0.000071
Endoplasmic Reticulum 0.002466 0.000548 0.003648 0.001764 0.001377 0.000698 0.000780 0.000959 0.001860 0.000673 0.001242 0.001220
Endosome 0.025281 0.044785 0.018275 0.008927 0.019111 0.039924 0.002937 0.005363 0.021845 0.040721 0.005407 0.006518
Golgi 0.007306 0.006156 0.000301 0.004958 0.005315 0.010113 0.000090 0.001992 0.006197 0.009464 0.000124 0.002953
Lipid Particles 0.010069 0.003607 0.005461 0.001351 0.007109 0.004465 0.001893 0.001063 0.008421 0.004324 0.002468 0.001156
Mitochondria 0.005143 0.001805 0.000203 0.004735 0.003292 0.005753 0.000189 0.002699 0.004112 0.005105 0.000192 0.003358
Mitotic Spindle 0.001889 0.000859 0.001083 0.043616 0.001138 0.007432 0.000724 0.014255 0.001471 0.006354 0.000782 0.023767
None 0.006287 0.002636 0.000904 0.005365 0.011453 0.001501 0.010067 0.002014 0.009164 0.001687 0.008591 0.003100
Nuclear Periphery 0.000341 0.000089 0.000821 0.000995 0.000310 0.000155 0.000626 0.000463 0.000324 0.000144 0.000657 0.000635
Nuclear Periphery Foci 0.000623 0.000741 0.010232 0.001408 0.000709 0.000478 0.000952 0.000824 0.000671 0.000521 0.002447 0.001013
Nucleolus 0.001626 0.000488 0.000482 0.000233 0.002957 0.001102 0.000368 0.000261 0.002367 0.001001 0.000386 0.000252
Nucleus 0.049265 0.013476 0.104254 0.065189 0.056505 0.021964 0.250038* 0.071103 0.053296 0.020571 0.226556* 0.069187
Peroxisomes 0.003190 0.009907 0.001151 0.002609 0.003010 0.008364 0.000263 0.002427 0.003090 0.008617 0.000406 0.002486
Vacuole 0.098181 0.114774 0.268752* 0.219686* 0.078772 0.111471 0.114535 0.168392 0.087374 0.112013 0.139375 0.185009
Vacuole Periphery 0.003068 0.002585 0.000520 0.006738 0.002422 0.003044 0.000118 0.001730 0.002708 0.002969 0.000183 0.003353

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.07 -5.55 -2.77 -1.84 -1.66 -3.56 1.86 -4.31 -0.38 -4.83 -4.24 -1.80 -4.60 -1.51 -4.33
Bud Neck 3.71 6.30 10.89 6.32 0.65 -8.53 8.61 2.22 10.33 -6.67 -5.35 12.94 8.61 12.06 -5.57
Bud Site -4.92 -0.42 -1.91 3.32 -0.47 -9.46 7.74 -6.29 2.74 -11.08 -10.02 6.19 -6.08 4.05 -10.30
Cell Periphery 7.31 -4.55 1.46 -2.58 4.82 1.01 -3.96 4.39 3.45 5.36 2.75 -4.28 5.24 1.57 5.62
Cytoplasm 4.13 4.53 7.01 0.85 -0.89 9.00 4.85 1.87 -7.39 -3.47 8.56 4.84 4.43 -4.39 -1.72
Cytoplasmic Foci -5.00 14.85 34.75 22.23 5.73 -3.73 33.88 35.01 39.22 -0.18 -4.64 42.08 49.22 44.96 3.16
Eisosomes 7.33 4.37 8.63 1.19 3.19 -2.18 -2.10 7.91 7.65 2.73 0.21 -1.81 12.66 7.21 2.74
Endoplasmic Reticulum 4.03 -2.70 1.45 -4.52 4.67 3.17 -0.38 2.41 -1.07 4.02 4.80 -0.24 2.87 -3.37 4.81
Endosome -3.85 3.83 14.48 8.30 6.65 -9.56 13.91 13.53 19.48 -0.24 -9.66 17.37 20.02 21.06 3.63
Golgi 1.36 13.94 6.29 3.91 -1.87 -5.56 13.91 8.39 10.78 -2.65 -4.40 19.86 10.93 11.12 -3.29
Lipid Particles 5.89 4.19 9.30 4.08 5.58 3.27 8.51 9.88 6.42 5.19 5.82 10.85 13.50 7.02 7.32
Mitochondria 3.54 5.71 3.15 -0.05 -2.55 -2.45 4.90 2.29 4.74 -3.02 -1.16 7.54 3.96 4.67 -3.94
Mitotic Spindle 1.20 0.60 -6.31 -6.79 -6.60 -4.06 -0.23 -6.28 -1.92 -6.22 -3.67 0.37 -8.76 -4.71 -9.02
None 1.93 5.47 2.17 -0.48 -3.29 6.43 1.90 6.19 -1.14 3.19 7.32 1.79 6.69 -1.57 2.65
Nuclear Periphery 2.06 -4.44 -5.73 -11.44 -1.92 3.84 -12.19 -6.70 -10.84 5.27 3.09 -10.04 -7.57 -15.12 2.75
Nuclear Periphery Foci -1.05 -8.29 -5.27 -3.11 7.73 1.84 -4.54 0.28 -1.40 4.43 1.43 -8.69 -2.14 -2.91 7.78
Nucleolus 3.01 4.85 5.91 1.65 2.23 2.33 3.56 3.69 3.34 2.03 2.75 4.76 5.04 3.60 2.97
Nucleus 12.71 -4.92 -10.47 -19.26 0.50 12.75 -20.88 -10.43 -25.10 16.27 16.34 -21.54 -15.08 -30.67 15.36
Peroxisomes -3.92 4.52 4.67 5.20 -0.06 -5.98 8.76 4.04 7.95 -4.72 -7.00 11.19 5.95 9.40 -4.71
Vacuole -2.67 -11.21 -25.28 -17.57 -1.30 -7.36 -12.46 -27.44 -21.22 -6.05 -6.78 -15.09 -36.34 -28.86 -7.80
Vacuole Periphery 0.93 10.03 0.93 0.28 -2.06 -1.44 6.43 3.35 5.87 -2.77 -0.78 11.19 3.41 4.08 -3.46
Standard name
Human Ortholog
Description GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (79%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rga2

Rga2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rga2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available