Standard name | |
---|---|
Human Ortholog | |
Description | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 3 | – | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | – | 2 | 5 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 9 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 6 | 13 | 6 | 12 | 24 | 16 | – | 17 | 11 | 8 | 8 | 0 | 5 | 0 | 3 | 5 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Cytoplasm | 69 | 276 | 205 | 182 | 157 | 165 | 208 | – | 203 | 176 | 114 | 105 | 171 | 226 | 240 | 217 | 157 | 140 | 79 | 278 | 193 | 124 | 252 | 282 |
Endoplasmic Reticulum | 0 | 1 | 2 | 6 | 10 | 12 | 7 | – | 2 | 2 | 2 | 0 | 1 | 3 | 1 | 10 | 6 | 9 | 1 | 2 | 5 | 14 | 30 | 27 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 1 | 1 | 3 | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 2 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 11 | 25 | 27 | 20 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 2 | – | 2 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 1 | 0 | 4 | 16 | – | 11 | 18 | 7 | 16 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 4 | 3 | 7 | 6 |
Unique Cell Count | 69 | 277 | 208 | 190 | 173 | 196 | 243 | 226 | 215 | 147 | 142 | 173 | 232 | 242 | 222 | 161 | 143 | 86 | 287 | 220 | 159 | 322 | 342 | |
Labelled Cell Count | 69 | 284 | 222 | 196 | 182 | 212 | 266 | 254 | 240 | 166 | 158 | 173 | 236 | 242 | 233 | 168 | 152 | 86 | 287 | 220 | 159 | 322 | 342 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 176.9 | 139.0 | 100.3 | 112.9 | 74.3 | 35.6 | 15.0 | – | 8.5 | 8.2 | 8.3 | 7.9 | 144.0 | 132.4 | 111.2 | 200.9 | 215.6 | 177.0 | 115.5 | 110.7 | 107.3 |
Std Deviation (1e-4) | 46.2 | 36.2 | 32.7 | 36.5 | 25.6 | 18.3 | 7.1 | – | 3.9 | 3.2 | 3.2 | 4.7 | 48.8 | 37.7 | 35.7 | 68.2 | 68.7 | 71.4 | 48.5 | 42.1 | 44.0 |
Intensity Change (Log2) | – | – | – | 0.17 | -0.43 | -1.5 | -2.74 | – | -3.56 | -3.61 | -3.6 | -3.67 | 0.52 | 0.4 | 0.15 | 1.0 | 1.1 | 0.82 | 0.2 | 0.14 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | 0.3 | 2.1 | 0.1 | – | 0.5 | -0.5 | -0.3 | -0.2 | -3.3 | -2.2 | -3.9 | -2.7 | -1.4 | -3.0 |
Cytoplasm | -1.7 | -3.5 | -5.2 | -4.9 | – | -3.8 | -5.7 | -6.5 | -7.1 | 0.2 | -0.8 | 0.6 | -0.6 | -0.7 | -0.5 |
Endoplasmic Reticulum | 0 | 2.7 | 2.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.2 | 5.1 | 6.4 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.0 | – | 2.4 | 3.6 | 0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 149.163 | 229.5001 | 201.1202 | 191.2439 | 164.1852 | 162.0249 | 174.9533 | 286.1682 | 260.2753 | 259.748 | 221.6409 | 240.4365 | 173.8485 | 258.4984 | 228.3718 | 205.6086 | 176.7723 | 244.644 |
Actin | 0.0131 | 0.0006 | 0.0047 | 0.0549 | 0.004 | 0.0137 | 0.0139 | 0.0006 | 0.0014 | 0.0005 | 0.0069 | 0.0017 | 0.0136 | 0.0005 | 0.0118 | 0.0004 | 0.0004 | 0.0015 |
Bud | 0.0035 | 0.0015 | 0.0023 | 0.0028 | 0.0043 | 0.0201 | 0.0079 | 0.0021 | 0.0034 | 0.0086 | 0.007 | 0.0088 | 0.0026 | 0.0025 | 0.0023 | 0.0027 | 0.0043 | 0.0035 |
Bud Neck | 0.0014 | 0.0012 | 0.0019 | 0.0025 | 0.0019 | 0.0063 | 0.0011 | 0.0009 | 0.001 | 0.0013 | 0.0022 | 0.003 | 0.0011 | 0.0007 | 0.0014 | 0.0005 | 0.0008 | 0.0024 |
Bud Periphery | 0.0037 | 0.0015 | 0.0017 | 0.0022 | 0.0072 | 0.0102 | 0.0102 | 0.0012 | 0.0044 | 0.0094 | 0.0102 | 0.0134 | 0.0027 | 0.0017 | 0.0031 | 0.0027 | 0.0031 | 0.0029 |
Bud Site | 0.0053 | 0.0014 | 0.0066 | 0.0033 | 0.0012 | 0.0022 | 0.0036 | 0.0016 | 0.0015 | 0.0026 | 0.001 | 0.0005 | 0.0013 | 0.0013 | 0.0012 | 0.0008 | 0.0013 | 0.0004 |
Cell Periphery | 0.0587 | 0.0558 | 0.0662 | 0.0554 | 0.0337 | 0.0127 | 0.0665 | 0.0365 | 0.0536 | 0.0371 | 0.0214 | 0.0336 | 0.0751 | 0.047 | 0.0578 | 0.0796 | 0.0354 | 0.0218 |
Cytoplasm | 0.3798 | 0.7639 | 0.5588 | 0.4864 | 0.3806 | 0.4497 | 0.4477 | 0.8342 | 0.7273 | 0.4611 | 0.6178 | 0.5573 | 0.4902 | 0.8119 | 0.6558 | 0.6183 | 0.4742 | 0.7597 |
Cytoplasmic Foci | 0.0517 | 0.0006 | 0.0223 | 0.003 | 0.006 | 0.0122 | 0.0169 | 0.0007 | 0.0058 | 0.0018 | 0.0025 | 0.0056 | 0.0201 | 0.001 | 0.0041 | 0.0014 | 0.0052 | 0.0034 |
Eisosomes | 0.0027 | 0 | 0.0014 | 0.001 | 0.0001 | 0.0006 | 0.0039 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0016 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0142 | 0.0051 | 0.0138 | 0.0016 | 0.0118 | 0.0251 | 0.0229 | 0.0024 | 0.0064 | 0.0043 | 0.0025 | 0.007 | 0.024 | 0.009 | 0.0138 | 0.0078 | 0.0029 | 0.0272 |
Endosome | 0.0096 | 0.002 | 0.0046 | 0.0046 | 0.0217 | 0.0354 | 0.0122 | 0.001 | 0.0029 | 0.0044 | 0.0071 | 0.0152 | 0.0088 | 0.0011 | 0.0057 | 0.0018 | 0.0058 | 0.0083 |
Golgi | 0.0026 | 0.0003 | 0.0012 | 0.0063 | 0.006 | 0.0067 | 0.0023 | 0.0001 | 0.0005 | 0.0002 | 0.0027 | 0.001 | 0.0039 | 0.0003 | 0.0024 | 0.0002 | 0.0005 | 0.0013 |
Lipid Particles | 0.008 | 0.0012 | 0.0068 | 0.0044 | 0.0377 | 0.0045 | 0.0077 | 0.001 | 0.0032 | 0.0014 | 0.0026 | 0.0031 | 0.0137 | 0.0022 | 0.0038 | 0.0061 | 0.0025 | 0.0031 |
Mitochondria | 0.0277 | 0.0018 | 0.0105 | 0.0114 | 0.0274 | 0.0589 | 0.0268 | 0.0009 | 0.0062 | 0.0048 | 0.0471 | 0.0075 | 0.0145 | 0.0023 | 0.0056 | 0.0063 | 0.0264 | 0.0043 |
None | 0.0307 | 0.0004 | 0.0063 | 0.0019 | 0.0014 | 0.0111 | 0.0142 | 0.0008 | 0.0051 | 0.0018 | 0.0259 | 0.004 | 0.0128 | 0.0009 | 0.0067 | 0.0023 | 0.0034 | 0.0014 |
Nuclear Periphery | 0.0483 | 0.0111 | 0.0199 | 0.0148 | 0.0099 | 0.0628 | 0.0677 | 0.0051 | 0.0094 | 0.016 | 0.0123 | 0.0115 | 0.0275 | 0.0062 | 0.0125 | 0.0073 | 0.0038 | 0.0134 |
Nucleolus | 0.0197 | 0.0013 | 0.0101 | 0.0212 | 0.005 | 0.0533 | 0.0236 | 0.0033 | 0.0036 | 0.0053 | 0.02 | 0.0411 | 0.0401 | 0.0023 | 0.0095 | 0.0196 | 0.0763 | 0.0037 |
Nucleus | 0.0467 | 0.0081 | 0.0116 | 0.0272 | 0.017 | 0.0696 | 0.0389 | 0.0116 | 0.0117 | 0.0199 | 0.0221 | 0.014 | 0.0206 | 0.0051 | 0.0083 | 0.012 | 0.0165 | 0.0135 |
Peroxisomes | 0.0032 | 0.0001 | 0.0023 | 0.0009 | 0.0173 | 0.0014 | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0073 | 0.0001 | 0.0036 | 0.0002 | 0.0009 | 0.0002 |
Punctate Nuclear | 0.0189 | 0 | 0.0025 | 0.0004 | 0.0019 | 0.0039 | 0.0064 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0014 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0003 |
Vacuole | 0.196 | 0.121 | 0.2087 | 0.223 | 0.3083 | 0.0911 | 0.1692 | 0.087 | 0.1372 | 0.3347 | 0.14 | 0.1989 | 0.1877 | 0.0947 | 0.1706 | 0.1837 | 0.2407 | 0.0978 |
Vacuole Periphery | 0.0544 | 0.0212 | 0.0358 | 0.0707 | 0.0955 | 0.0485 | 0.0348 | 0.0088 | 0.0145 | 0.0847 | 0.047 | 0.0718 | 0.0291 | 0.0091 | 0.0185 | 0.0457 | 0.0954 | 0.0299 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4233.2074 | 4459.9492 | 4689.122 | 3752.9901 | 3976.1852 | 4012.6163 | 3927.0862 | 3409.9483 | 3784.5883 | 3217.135 |
Translational Efficiency | 0.474 | 0.4979 | 0.5066 | 0.5992 | 0.5169 | 0.4444 | 0.5078 | 0.555 | 0.4662 | 0.5093 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1071 | 1283 | 808 | 1372 | 1469 | 2790 | 2035 | 462 | 2540 | 4073 | 2843 | 1834 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 614.50 | 670.54 | 807.28 | 783.91 | 658.49 | 696.19 | 776.78 | 798.33 | 639.94 | 688.11 | 785.45 | 787.54 |
Standard Deviation | 74.81 | 115.77 | 91.45 | 113.27 | 72.60 | 88.76 | 92.23 | 124.88 | 76.68 | 98.79 | 93.03 | 116.47 |
Intensity Change Log 2 | — | 0.125910 | 0.393656 | 0.351275 | — | 0.080320 | 0.238345 | 0.277824 | — | 0.102507 | 0.315406 | 0.313747 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000121 | 0.000668 | 0.001610 | 0.001988 | 0.000105 | 0.000511 | 0.000290 | 0.000483 | 0.000112 | 0.000560 | 0.000665 | 0.001608 |
Bud Neck | 0.015469 | 0.013122 | 0.008534 | 0.019561 | 0.011995 | 0.026828 | 0.017230 | 0.010821 | 0.013460 | 0.022511 | 0.014758 | 0.017359 |
Bud Site | 0.004399 | 0.008786 | 0.017643 | 0.068301 | 0.001767 | 0.012999 | 0.024531 | 0.020298 | 0.002877 | 0.011672 | 0.022574 | 0.056208 |
Cell Periphery | 0.000128 | 0.000135 | 0.000201 | 0.000269 | 0.000147 | 0.000102 | 0.000193 | 0.000194 | 0.000139 | 0.000112 | 0.000196 | 0.000250 |
Cytoplasm | 0.293429* | 0.236173* | 0.136228* | 0.359404* | 0.291184* | 0.177124* | 0.377922* | 0.414052* | 0.292131* | 0.195725* | 0.309231* | 0.373170* |
Cytoplasmic Foci | 0.413044* | 0.322396* | 0.011693 | 0.025904 | 0.368337* | 0.359900* | 0.021227 | 0.035933 | 0.387188* | 0.348086* | 0.018517 | 0.028431 |
Eisosomes | 0.000143 | 0.000123 | 0.000093 | 0.000050 | 0.000155 | 0.000102 | 0.000048 | 0.000060 | 0.000150 | 0.000109 | 0.000061 | 0.000052 |
Endoplasmic Reticulum | 0.000539 | 0.000896 | 0.004304 | 0.002639 | 0.000703 | 0.000456 | 0.001160 | 0.003383 | 0.000634 | 0.000594 | 0.002053 | 0.002826 |
Endosome | 0.023543 | 0.035811 | 0.005045 | 0.012380 | 0.018454 | 0.032614 | 0.004217 | 0.012802 | 0.020600 | 0.033621 | 0.004452 | 0.012487 |
Golgi | 0.006129 | 0.011223 | 0.000895 | 0.010604 | 0.005309 | 0.016277 | 0.001146 | 0.015382 | 0.005655 | 0.014685 | 0.001074 | 0.011808 |
Lipid Particles | 0.034176 | 0.036034 | 0.003648 | 0.004101 | 0.055283 | 0.038156 | 0.002069 | 0.003276 | 0.046383 | 0.037488 | 0.002518 | 0.003893 |
Mitochondria | 0.011467 | 0.016242 | 0.001161 | 0.007176 | 0.009491 | 0.027371 | 0.001495 | 0.007868 | 0.010324 | 0.023865 | 0.001400 | 0.007351 |
Mitotic Spindle | 0.025717 | 0.123261* | 0.192932* | 0.181207* | 0.030822 | 0.176617* | 0.104283* | 0.092031 | 0.028670 | 0.159810* | 0.129478* | 0.158743* |
None | 0.009833 | 0.015087 | 0.016483 | 0.005248 | 0.015893 | 0.006401 | 0.021394 | 0.008653 | 0.013338 | 0.009137 | 0.019998 | 0.006106 |
Nuclear Periphery | 0.000881 | 0.001395 | 0.002010 | 0.002791 | 0.001364 | 0.000874 | 0.001846 | 0.006377 | 0.001160 | 0.001038 | 0.001893 | 0.003694 |
Nuclear Periphery Foci | 0.003358 | 0.006730 | 0.010434 | 0.003029 | 0.003899 | 0.002069 | 0.002071 | 0.015094 | 0.003671 | 0.003538 | 0.004448 | 0.006068 |
Nucleolus | 0.012107 | 0.022637 | 0.052482 | 0.001983 | 0.020880 | 0.013776 | 0.013283 | 0.004064 | 0.017181 | 0.016568 | 0.024424 | 0.002507 |
Nucleus | 0.099991 | 0.087901 | 0.512591* | 0.196855* | 0.126411 | 0.043252 | 0.375889* | 0.204571* | 0.115271 | 0.057316 | 0.414741* | 0.198799* |
Peroxisomes | 0.027667 | 0.038881 | 0.003205 | 0.004340 | 0.022962 | 0.048207 | 0.001370 | 0.001652 | 0.024946 | 0.045270 | 0.001891 | 0.003663 |
Vacuole | 0.017066 | 0.020420 | 0.018418 | 0.083806 | 0.014159 | 0.014827 | 0.028024 | 0.133580 | 0.015385 | 0.016589 | 0.025294 | 0.096344 |
Vacuole Periphery | 0.000793 | 0.002079 | 0.000390 | 0.008365 | 0.000680 | 0.001536 | 0.000313 | 0.009424 | 0.000728 | 0.001707 | 0.000335 | 0.008632 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.35 | -12.06 | -9.14 | -3.81 | 3.09 | -3.62 | -14.84 | -11.27 | -4.99 | -3.71 | -4.24 | -17.48 | -10.92 | -6.30 | -1.93 |
Bud Neck | 0.36 | -3.27 | -5.09 | -6.20 | -2.29 | -9.38 | -6.92 | -2.05 | 5.38 | 3.52 | -8.17 | -7.38 | -6.58 | 0.58 | 0.17 |
Bud Site | -2.81 | -5.76 | -10.63 | -8.99 | -4.49 | -9.06 | -10.54 | -5.90 | -2.85 | 0.13 | -8.85 | -11.77 | -12.34 | -8.88 | -4.20 |
Cell Periphery | -0.66 | -4.60 | -4.37 | -4.27 | -0.27 | 4.06 | -3.48 | -3.80 | -6.44 | -1.47 | 2.41 | -5.43 | -5.57 | -7.67 | -1.68 |
Cytoplasm | 2.07 | 17.49 | -5.67 | -8.33 | -26.76 | 5.96 | -0.42 | -3.64 | -7.58 | -3.49 | 6.32 | 6.18 | -7.40 | -13.91 | -13.41 |
Cytoplasmic Foci | 12.34 | 52.31 | 49.61 | 39.99 | -7.25 | 3.39 | 49.57 | 47.45 | 57.71 | 1.84 | 9.85 | 69.92 | 69.81 | 75.20 | 1.07 |
Eisosomes | 1.68 | -4.94 | 3.86 | 2.27 | 7.26 | 6.50 | 3.66 | 1.12 | -4.14 | -1.67 | 6.59 | 0.12 | 4.91 | -1.93 | 4.25 |
Endoplasmic Reticulum | -2.72 | -18.24 | -18.18 | -17.76 | 4.87 | 1.86 | -18.79 | -12.42 | -12.72 | -7.03 | -0.47 | -25.11 | -22.47 | -22.72 | -4.94 |
Endosome | -4.75 | 8.11 | 10.10 | 14.65 | 2.30 | -7.85 | 7.96 | 7.41 | 13.85 | 1.35 | -8.61 | 11.18 | 13.92 | 22.06 | 3.84 |
Golgi | -3.75 | 7.02 | 3.51 | 6.32 | -0.90 | -9.56 | 8.77 | 1.38 | 5.77 | -0.93 | -10.07 | 11.16 | 3.70 | 10.54 | -1.57 |
Lipid Particles | 1.33 | 13.40 | 13.24 | 13.08 | -0.16 | 3.62 | 16.37 | 15.95 | 18.66 | -1.59 | 3.32 | 20.80 | 20.28 | 22.47 | -1.24 |
Mitochondria | -1.90 | 5.09 | 3.48 | 5.51 | -4.64 | -8.96 | 5.41 | 3.72 | 12.56 | -0.58 | -8.75 | 7.40 | 5.10 | 14.62 | -3.82 |
Mitotic Spindle | -11.41 | -18.61 | -10.12 | 1.29 | 10.90 | -16.39* | -19.33 | -4.45 | 4.51 | 7.96 | -20.23 | -26.46 | -11.38 | 3.85 | 12.06 |
None | -2.12 | 0.26 | 2.15 | 4.58 | 2.51 | 6.79 | -0.16 | 3.97 | -1.23 | 4.66 | 4.61 | -0.61 | 5.18 | 0.75 | 6.93 |
Nuclear Periphery | -2.04 | -13.51 | -16.51 | -14.41 | -9.22 | 1.79 | -14.80 | -7.12 | -7.59 | -2.45 | -0.06 | -19.81 | -17.49 | -17.54 | -8.62 |
Nuclear Periphery Foci | -0.92 | -8.83 | 0.92 | 1.99 | 9.64 | 6.59 | -2.48 | -0.81 | -4.55 | 0.85 | 4.33 | -7.79 | 0.72 | -2.97 | 7.95 |
Nucleolus | -2.63 | -12.20 | 9.17 | 12.20 | 18.01 | 9.00 | -4.52 | 13.59 | 7.78 | 18.43* | 6.18 | -10.89 | 16.22 | 13.51 | 25.30* |
Nucleus | 0.27 | -19.40 | -25.75 | -26.30 | 2.50 | 11.27 | -26.02 | -13.71 | -18.06 | 1.87 | 9.65 | -33.17 | -28.88 | -35.44 | 2.42 |
Peroxisomes | -3.20 | 16.57 | 17.72 | 17.96 | 2.32 | -8.82 | 19.61 | 21.64 | 30.31* | 5.29 | -9.00 | 25.69 | 26.95 | 34.24 | 4.02 |
Vacuole | -2.89 | -5.09 | -24.95 | -24.13 | -22.49 | -2.49 | -12.78 | -17.78 | -17.60 | -15.21 | -3.47 | -13.55 | -30.54 | -30.03 | -25.06 |
Vacuole Periphery | -3.77 | 1.46 | -2.52 | -0.97 | -2.75 | -6.15 | 3.79 | -1.65 | -0.80 | -1.86 | -6.97 | 3.87 | -3.06 | -1.44 | -3.42 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|