Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 – 0.18 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0.07 0.06 0.06 – 0.06 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.07 0 0.05 0.06 0 0 0 0 0 0.05 0 – 0.06 – 0 0 0 0 0.07 0.05
Golgi 0.05 0 0.16 0.19 0.13 0 0 0.06 0 0 0 0 0.21 0.22 0.28 – 0.35 – 0.05 0.1 0.05 0.09 0.17 0.08
Mitochondria 0.89 0.94 0.77 0.79 0.7 0.8 0.75 0.63 0.89 0.88 0.84 0.89 0.82 0.79 0.8 – 0.06 – 0.8 0.76 0.79 0.63 0.66 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0.06 0.07 0.05 0 0 0.08 0.08 0.06 0.07 0.08 0.08 0.1 0.07 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.05 0.07 0.05 0.06 0.07 0.07 0.08 0.07 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0.05 0.07 0 0 0 0 0 0 0 0 0.06 0 – 0 – 0.05 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.11 0.11 0.18 0.12 0.15 0.21 0.08 0.06 0.08 0.08 0.07 0.1 0.08 – 0.29 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 3 6 0 0 0 2 0 0 0 0 1 1 1 – 0 – 1 3 1 2 2 2
Bud 2 2 8 11 3 4 7 8 15 16 14 10 0 1 0 – 1 – 0 0 0 0 0 0
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 0 7 6 3 0 0 0 0 – 0 – – – – – – –
Cell Periphery 6 6 13 11 4 9 6 10 7 4 3 4 1 7 1 – 3 – 0 0 0 0 0 0
Cytoplasm 3 0 8 16 17 4 19 18 11 9 11 4 2 8 7 – 1 – 0 0 5 2 1 0
Endoplasmic Reticulum 2 4 2 2 0 3 2 2 0 0 0 0 0 0 3 – 0 – 0 0 1 1 0 0
Endosome 6 2 10 13 26 18 23 33 10 9 7 10 1 6 5 – 1 – 10 6 11 3 8 6
Golgi 15 6 40 53 46 15 16 30 0 0 3 4 6 28 32 – 6 – 15 16 14 7 20 10
Mitochondria 243 147 191 217 248 374 339 322 404 385 287 360 23 100 92 – 1 – 230 126 236 52 81 90
Nucleus 2 0 2 0 1 3 5 6 2 3 1 3 0 0 0 – 0 – 0 0 0 1 0 0
Nuclear Periphery 16 11 12 3 9 38 34 30 33 33 27 41 2 1 2 – 0 – 2 0 0 1 0 2
Nucleolus 18 4 3 7 17 34 24 29 31 30 27 27 1 2 1 – 0 – 2 0 0 2 1 0
Peroxisomes 1 2 30 15 25 11 4 14 10 14 5 4 0 8 3 – 0 – 15 6 20 3 2 4
SpindlePole 2 0 4 5 7 10 8 15 8 11 6 5 0 0 0 – 0 – 2 0 0 1 0 0
Vac/Vac Membrane 31 17 28 30 64 56 67 105 34 25 27 31 2 13 9 – 5 – 3 7 1 1 1 5
Unique Cell Count 273 157 249 275 355 468 451 508 452 439 341 406 28 126 115 17 287 167 299 83 125 130
Labelled Cell Count 350 205 354 389 467 579 556 624 572 545 421 503 39 175 156 18 287 167 299 83 125 130


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 7.4 9.2 9.5 9.8 8.5 8.3 9.1 7.4 7.4 7.5 7.3 8.6 9.6 8.5 – 7.8 – 10.5 11.8 10.4
Std Deviation (1e-4) 1.6 1.1 2.0 2.1 1.8 1.7 1.8 2.1 2.0 1.6 2.2 1.6 1.2 1.7 1.6 – 1.0 – 1.9 11.4 2.0
Intensity Change (Log2) – – – 0.05 0.09 -0.1 -0.15 -0.01 -0.32 -0.3 -0.28 -0.33 -0.09 0.06 -0.12 – -0.23 – 0.19 0.36 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud 0.5 0 0 -1.5 -1.5 0.1 0.3 0.6 -0.6 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery -0.7 -3.0 -2.4 -3.0 -2.4 -2.8 -3.5 -3.2 -3.3 0 0.1 0 – 0 –
Cytoplasm 1.4 1.0 0 0.7 0.2 -0.6 -0.9 0 0 0 1.4 0 – 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Endosome 0.4 1.7 -0.1 0.6 1.4 -1.4 -1.5 -1.4 -1.1 0 0.3 0 – 0 –
Golgi 1.0 -1.1 -6.2 -5.8 -4.5 -8.8 -8.7 -7.0 -7.5 0 1.5 2.6 – 0 –
Mitochondria 0.6 -1.9 1.0 -0.5 -3.7 4.5 3.8 2.3 4.1 0.7 0.6 0.7 – -6.3 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nuclear Periphery -2.6 -1.5 1.7 1.4 0.6 1.3 1.4 1.5 2.4 0 0 0 – 0 –
Nucleolus 0 2.4 3.5 2.7 2.9 3.3 3.3 3.7 3.2 0 0 0 – 0 –
Peroxisomes -2.7 -2.1 -5.3 -6.6 -5.1 -5.4 -4.6 -5.4 -6.2 0 -1.7 -2.9 – 0 –
SpindlePole 0 0 0 0 1.1 0 0.8 0 0 0 0 0 – 0 –
Vacuole -0.1 2.3 0.3 1.3 3.2 -1.7 -2.6 -1.4 -1.6 0 -0.3 -1.0 – 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7051 8.1826 8.2354 7.5897 7.4628 8.1576 10.2763 10.796 10.1238 10.6787 10.8906 10.5086 9.7652 9.6255 9.5403 9.1238 8.5045 9.3463
Actin 0.0073 0.004 0.0011 0.0011 0.0004 0.0005 0.0034 0.0011 0.018 0.0013 0.001 0.0015 0.0099 0.0011 0.0037 0.0007 0.0002 0.0005
Bud 0.0001 0.0001 0.0013 0.0001 0 0 0.0002 0.0001 0.0003 0.0062 0.0005 0 0.0003 0.0001 0.0001 0.0001 0 0
Bud Neck 0.0001 0 0 0 0 0 0.0002 0 0.0002 0.0001 0 0 0.0001 0.0001 0 0 0 0
Bud Periphery 0.0004 0.0001 0.0014 0.0002 0 0 0.0004 0.0001 0.0009 0.0321 0.0021 0.0001 0.0005 0.0002 0.0001 0.0002 0.0001 0
Bud Site 0.0002 0.0002 0.001 0.0001 0 0 0.0005 0.0002 0.0014 0.0006 0.0001 0 0.0018 0.0002 0.0001 0.0002 0 0
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0002 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0001 0 0 0 0 0 0.0008 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0023 0.0019 0.0003 0.0002 0.0006 0.0003 0.012 0.0006 0.0008 0.0004 0.0001 0.0002 0.0014 0.0008 0.0013 0.0066 0.0008 0.0003
Eisosomes 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0005 0 0 0 0 0 0.0005 0 0.0002 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endosome 0.0057 0.0065 0.0006 0.0005 0.0035 0.0027 0.0193 0.0016 0.005 0.0028 0.0004 0.002 0.0057 0.0026 0.0031 0.0118 0.0048 0.0009
Golgi 0.0117 0.0197 0.0024 0.001 0.0265 0.0091 0.0198 0.0026 0.004 0.0049 0.0008 0.0045 0.0082 0.0062 0.0143 0.0124 0.0078 0.0092
Lipid Particles 0.0227 0.0084 0.0013 0.0008 0.0112 0.0035 0.0299 0.0057 0.0047 0.0012 0.0002 0.0014 0.008 0.0078 0.0087 0.0028 0.0053 0.0058
Mitochondria 0.9269 0.9493 0.9841 0.9906 0.9528 0.977 0.8606 0.9764 0.9509 0.934 0.9931 0.9752 0.9435 0.9733 0.9508 0.9589 0.9713 0.9798
None 0.0004 0 0 0 0 0 0.0007 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0056 0 0 0 0 0.0001 0.0013 0 0.0012 0.0002 0 0.0003 0.0003 0 0.0001 0 0 0
Nucleolus 0.0003 0.0001 0.0001 0 0.0001 0 0.0006 0.0001 0.0001 0.0004 0 0 0.0018 0.0001 0.0001 0 0 0
Nucleus 0.0002 0 0 0 0 0 0.0017 0 0.0002 0.0003 0 0 0.0005 0 0 0 0 0
Peroxisomes 0.0108 0.0063 0.004 0.0039 0.0033 0.0012 0.024 0.0073 0.0081 0.002 0.001 0.0089 0.0089 0.004 0.0144 0.0043 0.0038 0.0013
Punctate Nuclear 0.0005 0 0 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0 0 0 0
Vacuole 0.0008 0.0008 0.0007 0.0003 0.0002 0.0006 0.0109 0.0008 0.0011 0.007 0.0002 0.0007 0.0028 0.0011 0.0006 0.0005 0.0012 0.0002
Vacuole Periphery 0.0031 0.0022 0.0014 0.0009 0.0011 0.0046 0.0124 0.0031 0.0017 0.0056 0.0003 0.005 0.0054 0.0021 0.0023 0.0009 0.0043 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.1114 18.6814 10.1657 17.4606 7.2253 13.9242 18.8887 26.3471 26.6187 18.295
Translational Efficiency 1.4245 1.6822 2.5644 1.2616 3.4922 2.7657 2.036 1.7478 1.6234 2.1954

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 759 761 2278 1516 2791 755 1658 1593 3550 1516 3936

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1026.05 1035.75 1247.38 1198.51 810.37 1121.18 1233.89 1254.56 820.80 1102.91 1240.66 1222.12
Standard Deviation 195.83 165.48 207.68 189.35 156.42 162.96 194.48 206.23 165.16 167.21 201.33 198.58
Intensity Change Log 2 — 0.013575 0.281800 0.224141 — 0.468365 0.606561 0.630529 — 0.232083 0.434183 0.417692

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.017043 0.006480 0.005923 0.002620 0.002593 0.001170 0.003192 0.008121 0.003292 0.002305 0.004563 0.004937
Bud Neck 0.002938 0.001749 0.006903 0.000862 0.003434 0.000187 0.005478 0.000841 0.003410 0.000521 0.006193 0.000854
Bud Site 0.032022 0.005729 0.011827 0.004959 0.007159 0.000264 0.006239 0.002470 0.008360 0.001433 0.009044 0.003911
Cell Periphery 0.001272 0.000233 0.000250 0.000560 0.000906 0.000059 0.000179 0.000326 0.000924 0.000096 0.000215 0.000461
Cytoplasm 0.005237 0.003544 0.001619 0.000625 0.001902 0.000126 0.000999 0.000363 0.002063 0.000856 0.001311 0.000515
Cytoplasmic Foci 0.031166 0.022882 0.041847 0.007530 0.014704 0.007325 0.046077 0.006780 0.015500 0.010651 0.043954 0.007214
Eisosomes 0.000408 0.000167 0.000147 0.000092 0.000092 0.000058 0.000096 0.000189 0.000107 0.000081 0.000121 0.000133
Endoplasmic Reticulum 0.002861 0.000205 0.000529 0.000274 0.000389 0.000104 0.000408 0.000224 0.000508 0.000126 0.000469 0.000253
Endosome 0.045748 0.020214 0.036557 0.009188 0.017123 0.006685 0.030543 0.006452 0.018506 0.009578 0.033562 0.008035
Golgi 0.057451 0.036076 0.050078 0.033205 0.020738 0.021697 0.050987 0.031198 0.022512 0.024772 0.050531 0.032359
Lipid Particles 0.002722 0.006495 0.008618 0.000821 0.010596 0.002032 0.006588 0.000752 0.010215 0.002986 0.007607 0.000792
Mitochondria 0.722242* 0.851132* 0.750679* 0.909587* 0.882928* 0.950884* 0.755921* 0.924449* 0.875161* 0.929557* 0.753289* 0.915847*
Mitotic Spindle 0.004955 0.002876 0.021113 0.003806 0.001608 0.000557 0.025286 0.001676 0.001770 0.001053 0.023191 0.002909
None 0.000579 0.000368 0.000865 0.000550 0.001224 0.000227 0.000536 0.000491 0.001193 0.000258 0.000701 0.000526
Nuclear Periphery 0.000424 0.000164 0.002375 0.000181 0.000139 0.000052 0.001201 0.000077 0.000152 0.000076 0.001791 0.000137
Nuclear Periphery Foci 0.001607 0.000195 0.001540 0.000164 0.000340 0.000063 0.001031 0.000091 0.000402 0.000091 0.001287 0.000133
Nucleolus 0.001294 0.000125 0.001166 0.000144 0.000798 0.000025 0.000610 0.000107 0.000822 0.000046 0.000889 0.000129
Nucleus 0.028384 0.000112 0.000761 0.000043 0.000780 0.000019 0.000425 0.000034 0.002114 0.000039 0.000594 0.000039
Peroxisomes 0.010297 0.036582 0.023068 0.005890 0.026486 0.004861 0.035687 0.007697 0.025704 0.011643 0.029352 0.006651
Vacuole 0.019959 0.002248 0.018531 0.007848 0.003384 0.001525 0.014255 0.002785 0.004186 0.001679 0.016401 0.005715
Vacuole Periphery 0.011391 0.002423 0.015602 0.011052 0.002678 0.002079 0.014263 0.004880 0.003099 0.002153 0.014935 0.008452

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.21 1.28 1.67 2.98 3.47 2.96 -0.96 -4.10 -5.42 -3.68 1.47 -1.65 -1.98 -4.04 -0.49
Bud Neck 1.17 -2.65 2.09 2.37 5.48 4.43 -1.95 3.48 -4.79 5.53 4.11 -2.91 3.62 -2.87 7.72
Bud Site 2.00 1.52 2.07 0.34 2.75 5.16 0.34 3.17 -3.50 3.19 4.71 -0.47 2.88 -3.97 3.73
Cell Periphery 1.53 1.50 0.95 -1.05 -0.99 3.62 3.09 2.42 -5.15 -2.59 3.68 3.14 1.60 -2.02 -1.36
Cytoplasm 0.66 1.58 1.98 2.13 2.20 3.54 1.69 3.06 -6.42 4.49 2.11 1.40 2.97 0.94 3.38
Cytoplasmic Foci 1.15 -1.38 3.45 6.06 10.32 5.57 -8.16 5.73 0.60 10.57 3.52 -10.46 6.46 4.06 14.74
Eisosomes 1.21 1.32 1.59 2.71 2.80 4.81 -0.43 -4.34 -6.01 -4.08 2.04 -1.11 -1.51 -3.66 -0.77
Endoplasmic Reticulum 2.06 1.81 2.01 -1.22 3.19 4.63 -0.24 2.68 -2.88 2.47 4.47 0.43 2.92 -3.27 3.86
Endosome 2.27 0.82 3.30 4.60 9.27 7.00 -4.99 6.79 0.25 9.90 5.68 -6.48 6.85 1.78 13.31
Golgi 1.73 0.57 2.02 0.68 3.84 -0.42 -7.03 -3.37 -3.17 4.18 -1.01 -8.68 -4.08 -3.43 5.65
Lipid Particles -2.01 -3.14 1.29 5.23 6.98 6.63 2.32 7.81 3.60 4.90 5.82 1.77 7.87 6.16 8.35
Mitochondria -3.21 -0.78 -4.69 -5.50 -12.70 -9.88 9.40 -5.36 5.11 -13.22 -7.72 11.58 -5.68 3.26 -18.18
Mitotic Spindle 1.05 -4.66 0.49 -1.23 5.68 3.53 -7.06 -0.17 -3.15 7.03 2.23 -9.54 -2.77 -4.74 8.93
None 2.39 -2.22 0.33 -5.60 3.16 3.46 2.39 2.55 -11.06 1.37 3.41 1.78 2.44 -14.10 3.32
Nuclear Periphery 0.89 -3.62 0.85 -0.35 4.98 2.04 -5.83 1.41 -1.54 6.32 1.76 -6.76 0.36 -3.37 6.91
Nuclear Periphery Foci 1.87 0.14 1.90 0.56 6.32 4.29 -3.64 3.79 -1.01 5.18 4.33 -5.65 3.60 -1.51 8.11
Nucleolus 2.06 0.32 2.01 -0.28 2.54 1.95 0.40 1.73 -1.88 3.39 2.07 -0.19 1.82 -1.62 3.53
Nucleus 1.80 1.76 1.80 1.77 2.09 2.20 0.90 2.16 -1.35 2.43 2.42 1.77 2.41 -0.51 2.92
Peroxisomes -4.27 -2.42 0.97 7.15 5.84 8.73 -2.02 7.17 -2.45 6.98 5.57 -1.11 7.91 4.07 9.13
Vacuole 2.89 0.30 1.97 -6.11 4.74 3.30 -6.88 0.91 -2.58 7.37 4.13 -8.75 -2.05 -7.22 7.70
Vacuole Periphery 3.27 -1.34 0.11 -8.77 2.46 1.73 -7.45 -3.17 -4.09 5.64 2.74 -10.32 -8.15 -10.06 5.14
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrp20

Mrp20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available