Standard name
Description Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.05 0.05 0 0.05 0.05 0 0.05 0 0.06 0.08 0.14 0.12 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0.05 0.11 0.06 0.29 0.33 0.17 0.54 0.59 0.33 0.06 0 0 0 0 0.14 0 0 0 0 0 0
Nucleus 0.88 0.95 0.94 0.93 0.9 0.86 0.83 0.88 0.8 0.81 0.85 0.93 0.89 0.81 0.77 0.78 0.8 0.85 0.89 0.84 0.86 0.7 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.11 0 0.05 0.1 0.09 0.18 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud 1 1 0 0 0 1 5 5 4 9 15 0 0 0 0 0 0 0 1 0 0 6 9
Bud Neck 0 0 1 1 1 1 1 4 1 2 3 0 0 0 0 0 0 0 0 0 1 3 9
Bud Site 0 0 0 1 1 6 6 5 20 9 16 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 4 15 11 26 21 31 33 15 16 17 29 54 87 12 9 4 4 0 2 0 1 2
Endoplasmic Reticulum 2 0 0 0 0 0 1 0 0 0 0 0 0 3 2 4 0 0 0 0 0 3 2
Endosome 0 0 1 0 0 0 1 0 0 0 1 0 2 6 0 4 1 3 0 2 0 2 8
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1 0 0 1 0
Mitochondria 13 15 15 23 27 147 192 118 258 200 151 28 11 13 4 2 9 1 1 3 0 7 19
Nucleus 138 175 309 201 438 434 489 603 386 273 384 474 575 504 79 72 51 136 166 297 163 379 408
Nuclear Periphery 0 0 0 0 2 1 4 4 8 1 2 1 4 5 1 1 0 1 0 1 0 4 4
Nucleolus 2 2 0 1 0 1 3 1 1 7 4 4 6 8 0 0 0 5 2 5 1 23 50
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 6 6 3 10 10 17 2 2 10 0 2 0 1 0 0 1 1 1 1 5 14
Vac/Vac Membrane 3 4 3 0 14 6 5 10 9 1 3 3 20 23 11 7 7 2 8 34 16 96 139
Unique Cell Count 156 185 330 217 486 507 589 685 481 337 451 509 647 621 103 92 64 161 186 354 191 539 681
Labelled Cell Count 172 202 350 244 512 628 748 800 704 520 606 539 675 652 111 100 72 161 186 354 191 539 681


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.5 5.6 4.9 5.7 4.4 4.6 4.8 4.0 3.8 4.5 5.4 5.5 5.6 7.9 8.5 7.3 5.0 5.1 5.4
Std Deviation (1e-4) 0.9 0.9 1.2 1.2 1.3 1.1 1.0 1.0 0.8 1.1 0.9 1.2 1.3 1.4 1.8 2.1 2.3 1.0 1.1 1.2
Intensity Change (Log2) -0.17 0.04 -0.34 -0.28 -0.21 -0.49 -0.55 -0.29 -0.05 -0.02 0.01 0.5 0.61 0.4 -0.15 -0.14 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.5 -0.3 0.5 0.2 -1.1 0.1 -0.5 0.7 2.2 4.5 2.6 1.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 0.6 0 0 0 0 0 0 0.6 -2.6 -2.1 0 0 0
Nucleus -0.5 -1.8 -3.6 -4.6 -2.8 -5.3 -4.9 -3.7 -0.3 -2.4 -5.2 -4.9 -4.4 -3.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 0 0 0 0 0 0 0 2.1 2.5 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available